Abstract:
:We have constructed a recombinant baculovirus expressing high levels of the herpes simplex virus type 1 (HSV-1) glycoprotein E (gE) in Sf9 cells. The expressed gE migrated on gels as a double band with apparent molecular weights of 68 and 70 kDa. The recombinant gE was glycosylated based on its susceptibility to tunicamycin treatment and was transported to the membrane of Sf9 cells based on indirect immunofluorescence. Mice vaccinated with gE developed high serum titers of HSV-1-neutralizing antibodies based on plaque reduction assays. gE vaccination also induced a strong delayed type hypersensitivity (DTH) response to HSV-1. In addition, mice vaccinated with the recombinant gE were protected from both intraperitoneal and ocular lethal HSV-1 challenge. To our knowledge, this is the first report in which vaccination with gE was shown to induce high neutralizing antibody titers, a DTH response, or protection against lethal HSV-1 challenge.
journal_name
Virologyjournal_title
Virologyauthors
Ghiasi H,Kaiwar R,Nesburn AB,Slanina S,Wechsler SLdoi
10.1016/0042-6822(92)90500-osubject
Has Abstractpub_date
1992-06-01 00:00:00pages
469-76issue
2eissn
0042-6822issn
1096-0341pii
0042-6822(92)90500-Ojournal_volume
188pub_type
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