Abstract:
:The adenovirus E1A 243R oncoprotein encodes a potent transcription-repression function within the N-terminal 80 amino acids. Our proposed model of E1A repression predicts that E1A interacts with important cellular proteins on chromatin. Consistent with this idea, we report here that E1A proteins from in vivo formaldehyde cross-linked 293 cells are closely associated with chromatin even after several stringent purification steps including double isopycnic CsCl density gradient centrifugation and size exclusion chromatography. Likewise, E1A proteins expressed from virus during productive infection of HeLa cells are closely associated with chromatin starting at early times after infection. No other adenoviral proteins are necessary for E1A 243R protein to associate with chromatin. Analyses of chromatin from HeLa cells infected with adenovirus vectors expressing E1A 243R protein with deletions in different E1A functional domains indicate that sequences within the E1A N-terminal repression domain are needed for the majority of E1A's interactions with chromatin.
journal_name
Virologyjournal_title
Virologyauthors
Green M,Panesar NK,Loewenstein PMdoi
10.1016/j.virol.2007.10.021subject
Has Abstractpub_date
2008-02-05 00:00:00pages
1-7issue
1eissn
0042-6822issn
1096-0341pii
S0042-6822(07)00692-7journal_volume
371pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::Although herpesviruses have a wide host range and their genomes vary substantially in size, the nucleocapsid appears to be a conservative element of viral design. The capsid shell is icosahedrally symmetric (T = 16), and 125 nm in diameter and 15nm thick in the case of herpes simplex virus 1 (HSV-1). Channel catfish v...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0016
更新日期:1996-01-15 00:00:00
abstract::Viroids are small, nontranslatable pathogenic RNAs that replicate autonomously and traffic systemically in their host plants. We have used in situ hybridization to analyze the trafficking pattern of Potato spindle tuber viroid (PSTVd) in tomato and Nicotiana benthamiana. When PSTVd was inoculated onto the stem of a pl...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2000.0724
更新日期:2001-01-05 00:00:00
abstract::The technique of cDNA polymerase chain reaction was used to study the heterogeneity of adenovirus type 12 early region 1A mRNAs in infected and transformed cells. In addition to 13 S and 12 S mRNAs, three transcripts of 10 S, 9.5 S, and 9 S could be isolated from infected HeLa cells. A fourth transcript of 11 S was fo...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90125-b
更新日期:1990-12-01 00:00:00
abstract::Latent membrane protein 2A (LMP2A) is expressed in latent Epstein-Barr virus (EBV) infection. LMP2A functions to downregulate B-cell signal transduction and viral reactivation from latency in EBV-immortalized B cells in vitro, and acts to provide B cells with both a survival and developmental signal in vivo. Identific...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1999.0166
更新日期:2000-03-01 00:00:00
abstract::Most adenovirus transducing vectors have the cytomegalovirus major immediate-early (CMV) or the Rous sarcoma virus long terminal repeat (RSV) promoter driving expression of the transgene. Both of these promoters are highly active in transfection and transduction assays in 293 cells, in which transducing vectors are co...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2001.1211
更新日期:2001-12-05 00:00:00
abstract::The APOBEC3 enzyme family are host restriction factors that induce mutagenesis of HIV-1 proviral genomes through the deamination of cytosine to form uracil in nascent single-stranded (-)DNA. HIV-1 suppresses APOBEC3 activity through the HIV-1 protein Vif that induces APOBEC3 degradation. Here we compared two common po...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2018.11.004
更新日期:2019-01-15 00:00:00
abstract::The gene encoding the M1 protein of sonchus yellow net virus (SYNV), a plant rhabdovirus, has been sequenced and identified by Western blot analysis of SYNV proteins using antibodies directed against a fusion protein derived from a portion of the sequenced gene. The M1 gene is positioned between nucleotides 4039 and 5...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90289-4
更新日期:1990-11-01 00:00:00
abstract::Cytotoxic T-cell clones raised against X-31 (H3N2) influenza virus in C57BL/6 mice can be directed against an influenza A virus subtype specific determinant (1). A representative T-cell clone (A3.1) has been used in combination with a set of genetically typed recombinant viruses, to show that the A/PR/8/34 nucleoprote...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90413-6
更新日期:1984-03-01 00:00:00
abstract::The RNA dependent RNA polymerase of Sendai virus consists of a complex of the large (L) and phosphoprotein (P) subunits where L is thought to be responsible for all the catalytic activities necessary for viral RNA synthesis. We previously showed that the L protein forms an oligomer [Smallwood, S., Cevik, B., Moyer, S....
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2007.01.032
更新日期:2007-06-20 00:00:00
abstract::RNA copies, synthesized in vitro, of the virulent satellite (RNA C) of turnip crinkle virus (TCV) infect plants when coinoculated with helper virus RNA. RNA C is a small linear RNA of about 355 bases which intensifies TCV symptoms in infected plants. Full-length cDNA copies of the satellite were inserted in an express...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(87)90445-4
更新日期:1987-01-01 00:00:00
abstract::Bovine cell lines individually expressing two related herpesviral proteins, pseudorabies virus glycoprotein 50 and herpes simplex virus type 1 glycoprotein D, were examined for their susceptibility/resistance to infection with several alphaherpesviruses. Cell lines expressing gp50 or gD-1 resisted plaque formation by ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1269
更新日期:1993-05-01 00:00:00
abstract::To identify ranavirus virulence genes, we engineered Frog Virus 3 (FV3) knockout (KO) mutants defective for a putative viral caspase activation and recruitment domain-containing (CARD) protein (Δ64R-FV3) and a β-hydroxysteroid dehydrogenase homolog (Δ52L-FV3). Compared to wild type (WT) FV3, infection of Xenopus tadpo...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2015.07.011
更新日期:2015-11-01 00:00:00
abstract::West Nile virus (WNV) genome cyclization is mediated by two pairs of long-distance RNA/RNA interactions: the 5'CS/3'CSI (conserved sequence) and the 5'UAR/3'UAR (upstream AUG region) base pairings. Antisense peptide-conjugated phosphorodiamidate morpholino oligomers (PPMOs), designed to interfere with the 5'CS/3'CSI o...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.08.044
更新日期:2008-12-05 00:00:00
abstract::The location of nucleotide sequences important in determining the extent of cowpea mosaic virus M-RNA accumulation in cowpea protoplasts has been analyzed by deletion mutagenesis of full-length cDNA clones from which infectious transcripts can be produced in vitro. The results suggest that cis-acting sequences which d...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1175
更新日期:1993-04-01 00:00:00
abstract::Human cytomegalovirus (HCMV) glycoprotein B (gB) promotes virion entry into cells by fusing the virion envelope with the cellular membrane. We recently reported that UB cells (U373 glioblastoma cells constructed to produce HCMV gB constitutively) form multinucleate syncytia that are dependent on the density of gB in t...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.1290
更新日期:1995-06-01 00:00:00
abstract::Flock house virus (FHV) encodes a suppressor protein B2 to overcome antiviral RNA silencing during infection. Biochemical analyses have shown that a homodimer of B2 binds to double-stranded RNA to inhibit dicer-mediated cleavage of dsRNA and incorporation of small interfering RNAs into the RNA-induced silencing comple...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2012.05.023
更新日期:2012-10-25 00:00:00
abstract::We previously demonstrated a high permissiveness of HTLV-II-transformed T lymphocytes (C3) to human immunodeficiency virus (HIV) infection in vitro, and that this infection results in the lysis of cells (D.C. Montefiori and W.M. Mitchell (1986) Virology 155, 726-731). We now show that a small percentage of HIV-infecte...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(87)90008-0
更新日期:1987-10-01 00:00:00
abstract::The red clover necrotic mosaic dianthovirus (RCNMV) genome is split between two single-stranded RNA species termed RNA-1 and RNA-2. RNA-2 is required for infection of whole plants but is dispensable for infection and virion formation in protoplasts. We have used full-length cDNA clones of RNA-1 and -2 from which infec...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1004
更新日期:1993-01-01 00:00:00
abstract::Beet necrotic yellow vein virus (BNYVV) has a quadripartite plus-strand RNA genome in which the two smallest genome components, RNA 3 and 4, are not necessary for virus multiplication in leaves. Infectious transcripts of BNYVV RNA 3 and 4 have already been described (V. Ziegler-Graff, S. Bouzoubaa, I. Jupin, H. Guille...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90131-1
更新日期:1989-09-01 00:00:00
abstract::Murine leukemia virus (MuLV) genome encodes a protease (Y. Yoshinaka, I. Katoh, T.D. Copeland, and S. Oroszlan (1985), Proc. Natl. Acad. Sci. USA 82, 1618-1622), which has been shown to cause maturation, specified as morphological conversion from "immature" to "mature" form of virus cores. To examine whether "immature...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90161-8
更新日期:1985-09-01 00:00:00
abstract::In a previous study on the replication of Kunjin virus using immunoelectron microscopy (E. G. Westaway, J. M. Mackenzie, M. T. Kenney, M. K. Jones, and A. A. Khromykh, 1997, J. Virol. 71, 6650-6661), NS1 and NS3 were found associated with double-stranded RNA (dsRNA) within vesicle packets (VP) in infected Vero cells, ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9156
更新日期:1998-06-05 00:00:00
abstract::The subgroup II luteovirus barley yellow dwarf virus-RPV (BYDV-RPV) acts as a helper virus for a satellite RNA (satRPV RNA). The subgroup II luteovirus beet western yellows virus (BWYV) and the ST9-associated RNA (ST9a RNA), a BWYV-associated RNA that encodes a polymerase similar to those of subgroup I luteoviruses, w...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8532
更新日期:1997-05-12 00:00:00
abstract::During respiratory syncytial virus (RSV) infection there is a close physical interaction between the filamentous actin (F-actin) and the virus, involving both inclusion bodies and the virus filaments. This interaction appears to occur relatively early in the replication cycle, and can be detected from 8 h post-infecti...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2007.08.007
更新日期:2007-12-20 00:00:00
abstract::Rabies continues to poses serious threats to the public health in many countries. The development of novel inexpensive, safe and effective vaccines has become a high priority for rabies control worldwide. We previously generated a novel recombinant rabies vaccine by cloning rabies virus glycoprotein into a chimpanzee ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2019.07.022
更新日期:2019-10-01 00:00:00
abstract::Myeloid differential primary response protein (MyD88) is a critical component in the signaling cascade through Toll-like receptors (TLRs) and is induced by alpha interferon (IFN-alpha). To examine the role of MyD88 in the antiviral activity of IFN-alpha against hepatitis B virus (HBV), we established MyD88 stably expr...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2003.11.005
更新日期:2004-02-20 00:00:00
abstract::Herpes simplex virus mutant KG111 contains a nonsense mutation at codon 44 of the viral thymidine kinase (tk) gene and produces low amounts of a truncated tk polypeptide. We tested mutant KG111 and related viruses that specify varying amounts of similar truncated tk polypeptides for their sensitivities to antiviral nu...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90261-4
更新日期:1989-02-01 00:00:00
abstract::Transient expression studies using a Nicotiana benthamiana suspension cell-derived protoplast system have identified a minimal sequence that is necessary and sufficient for activation of the tomato golden mosaic virus coat protein (CP) promoter by the viral TrAP protein (also called AL2). The sequence has a bipartite ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2002.1757
更新日期:2003-01-20 00:00:00
abstract::A stem-loop structure, formed by a pair of inverted repeats during DNA replication, is a conserved feature at the origin of DNA replication among plant and animal viruses, bacteriophages and plasmids that replicate their genomes via the rolling-circle replication (RCR) mechanism. In this work, a head-to-tail tandem co...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2007.01.017
更新日期:2007-06-20 00:00:00
abstract::The Rice stripe virus (RSV) pc4 has been determined as the viral movement protein (MP). In this study, the pc4 gene was cloned into a movement-deficient Tobacco mosaic virus (TMV). The resulting hybrid TMV-pc4, in addition to spreading cell to cell in Nicotiana tabacum, moved systemically and induced foliar necrosis i...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2012.01.007
更新日期:2012-04-10 00:00:00
abstract::Porcine circovirus type 2 (PCV2) is an economically important cause of post-weaning multisystemic wasting syndrome (PMWS) in weanling piglets. Current commercial vaccines against PCV2 are highly effective. Yet, a recurring emergence of new genotypes in vaccinated herds necessitates a better understanding of protective...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2019.11.008
更新日期:2020-01-15 00:00:00