Resistance and susceptibility of bovine cells expressing herpesviral glycoprotein D homologs to herpesviral infections.


:Bovine cell lines individually expressing two related herpesviral proteins, pseudorabies virus glycoprotein 50 and herpes simplex virus type 1 glycoprotein D, were examined for their susceptibility/resistance to infection with several alphaherpesviruses. Cell lines expressing gp50 or gD-1 resisted plaque formation by the homologous virus more than by the heterologous viruses. Bovine cells expressing bovine herpesvirus 1 glycoprotein IV (gIV) were susceptible to infection with three other bovine herpesviruses: bovine herpesvirus 2, bovine herpesvirus 4 (BHV-4) and alcelaphine herpesvirus 1. One line of gIV-expressing cells was resistant to the formation of BHV-4 plaques, suggesting that a cell-associated factor may be responsible for inhibiting cell-to-cell spread.






Chase CC,Lohff C,Letchworth GJ 3rd




Has Abstract


1993-05-01 00:00:00
















  • Comparison of pseudorabies virus China reference strain with emerging variants reveals independent virus evolution within specific geographic regions.

    abstract::Pseudorabies virus (PRV) China reference strain Ea is genetically closely related to newly emerged variants; however, there is limited information about PRV Ea. Here, we compared PRV Ea with new variant strains by growth kinetics, genome sequencing, and protein expression analysis. Growth analysis showed that strain E...


    pub_type: 杂志文章


    authors: Wang X,Wu CX,Song XR,Chen HC,Liu ZF

    更新日期:2017-06-01 00:00:00

  • Sequence and analysis of the capsid protein of Nudaurelia capensis omega virus, an insect virus with T = 4 icosahedral symmetry.

    abstract::We have determined the nucleotide sequence of the RNA2 segment of the Nudaurelia capensis omega virus genome. It was found to consist of 2448 nucleotides and contained one long open reading frame (ORF) encoding the 644 residue capsid protein. The deduced amino acid sequence of this protein reveals a positively charged...


    pub_type: 杂志文章


    authors: Agrawal DK,Johnson JE

    更新日期:1992-10-01 00:00:00

  • Viral persistent infection affects both transcriptional and posttranscriptional regulation of neuron-specific molecule GAP43.

    abstract::Recently, we reported that in vitro and in vivo persistent infection of neurons by lymphocytic choriomeningitis virus (LCMV) downregulated GAP43 expression, a protein involved in neuronal plasticity associated with learning and memory. Here, we investigated the transcriptional and posttranscriptional events involved. ...


    pub_type: 杂志文章


    authors: Cao W,Oldstone MB,De La Torre JC

    更新日期:1997-04-14 00:00:00

  • Human papillomavirus types 16 E6 and E7 contribute differently to carcinogenesis.

    abstract::High-risk human papillomaviruses (HPVs) are etiologically implicated in human cervical cancer. Two viral genes, E6 and E7, are commonly found expressed in these cancer cells. We have previously shown that mice transgenic for the HPV-16 E6 gene or E7 gene, in which the E6 or E7 was expressed in the basal layer of epith...


    pub_type: 杂志文章


    authors: Song S,Liem A,Miller JA,Lambert PF

    更新日期:2000-02-15 00:00:00

  • Role of capsid sequence and immature nucleocapsid proteins p9 and p15 in Human Immunodeficiency Virus type 1 genomic RNA dimerization.

    abstract::HIV-1 genomic RNA (gRNA) dimerization is important for viral infectivity and is regulated by proteolytic processing of the Gag precursor protein (Pr55gag) under the direction of the viral protease. The processing occurs in successive steps and, to date, the step associated with formation of a wild-type (WT) level of g...


    pub_type: 杂志文章


    authors: Kafaie J,Dolatshahi M,Ajamian L,Song R,Mouland AJ,Rouiller I,Laughrea M

    更新日期:2009-03-01 00:00:00

  • The effect of inhibitors on the growth of the entomopoxvirus from Amsacta moorei in Lymantria dispar (gypsy moth) cells.

    abstract::Within the family of Poxviridae, the entomopoxviruses are the most distant relatives of the more well-known and intensively studied orthopoxviruses (vaccinia and variola). The growth of the entomopoxvirus from Amsacta moorei (AmEPV) has been characterized in cell culture and compared to that of vaccinia virus (VV), th...


    pub_type: 杂志文章


    authors: Winter J,Hall RL,Moyer RW

    更新日期:1995-08-20 00:00:00

  • Establishment and characterization of equine fibroblast cell lines transformed in vivo and in vitro by BPV-1: model systems for equine sarcoids.

    abstract::It is now widely recognized that BPV-1 and less commonly BPV-2 are the causative agents of equine sarcoids. Here we present the generation of equine cell lines harboring BPV-1 genomes and expressing viral genes. These lines have been either explanted from sarcoid biopsies or generated in vitro by transfection of prima...


    pub_type: 杂志文章


    authors: Yuan ZQ,Gault EA,Gobeil P,Nixon C,Campo MS,Nasir L

    更新日期:2008-04-10 00:00:00

  • Nuclear matrix protein SMAR1 represses HIV-1 LTR mediated transcription through chromatin remodeling.

    abstract::Nuclear Matrix and MARs have been implicated in the transcriptional regulation of host as well as viral genes but their precise role in HIV-1 transcription remains unclear. Here, we show that >98% of HIV sequences contain consensus MAR element in their promoter. We show that SMAR1 binds to the LTR MAR and reinforces t...


    pub_type: 杂志文章


    authors: Sreenath K,Pavithra L,Singh S,Sinha S,Dash PK,Siddappa NB,Ranga U,Mitra D,Chattopadhyay S

    更新日期:2010-04-25 00:00:00

  • Transforming potential of deletion mutants of the SV40 T antigen coding gene in Syrian hamster cells.

    abstract::Thymidine kinase-deficient syrian hamster cells were cotransfected with recombinant plasmids containing the thymidine kinase (TK) gene of Herpes Simplex Virus Type 1, and either intact or partially deleted SV40 T antigen-coding genes. The transformants were selected by their ability to grow in gHAT medium. After selec...


    pub_type: 杂志文章


    authors: Hirschhorn RR,Mercer WE,Liu HT,Baserga R

    更新日期:1984-04-15 00:00:00

  • Towards quality and order in human papillomavirus research.

    abstract::The International Human Papillomavirus (HPV) Reference Center supports quality and order in HPV research and diagnostics. Notably, the center assigns HPV type numbers to novel HPV types, maintains a reference clone repository, and issues international proficiency panels for HPV genotyping. The established HPV types, c...


    pub_type: 杂志文章


    authors: Mühr LSA,Eklund C,Dillner J

    更新日期:2018-06-01 00:00:00

  • Interferon-beta and interferon-gamma synergistically inhibit the replication of severe acute respiratory syndrome-associated coronavirus (SARS-CoV).

    abstract::Recent studies have shown that interferon-gamma (IFN-gamma) synergizes with IFN-alpha/beta to inhibit the replication of both RNA and DNA viruses. We investigated the effects of IFNs on the replication of two strains of severe acute respiratory syndrome-associated coronavirus (SARS-CoV). While treatment of Vero E6 cel...


    pub_type: 杂志文章


    authors: Sainz B Jr,Mossel EC,Peters CJ,Garry RF

    更新日期:2004-11-10 00:00:00

  • Visualization of the dynamic multimerization of human Cytomegalovirus pp65 in punctuate nuclear foci.

    abstract::The phosphorylated protein pp65 of human Cytomegalovirus (HCMV) is the predominant virion protein and the major tegument constituent. It plays important roles in HCMV infection and virion assembly. Live cell imaging and fluorescence recovery after photobleaching (FRAP) analysis showed that HCMV pp65 accumulated dynami...


    pub_type: 杂志文章


    authors: Cui Z,Zhang K,Zhang Z,Liu Y,Zhou Y,Wei H,Zhang XE

    更新日期:2009-09-30 00:00:00

  • Phenotypic and functional characterization of CD8(+) T cell clones specific for a mouse cytomegalovirus epitope.

    abstract::A series of CD8(+) T cell clones, specific for the IE1 epitope YPHFMPTNL, of the immediate-early protein 1 of the murine cytomegalovirus (MCMV) were generated in order to determine their protective activity against this infection and correlate their phenotypic markers with antiviral activity. We found that the adoptiv...


    pub_type: 杂志文章


    authors: Fernandez JA,Zavala F,Tsuji M

    更新日期:1999-03-01 00:00:00

  • Identification of a new neutralization epitope on VP7 of human serotype 2 rotavirus and evidence for electropherotype differences caused by single nucleotide substitutions.

    abstract::Neutralizing monoclonal antibodies 2F1 and 1C10, which are specific for VP7 of serotype 2 rotaviruses (G2), were used to select neutralization escape variants of the human serotype 2 rotavirus, DS-1. Neutralization survival patterns generated by monoclonal antibodies 2F1, 1C10, and RV5:3 indicated that 2F1 and 1C10 di...


    pub_type: 杂志文章


    authors: Dunn SJ,Ward RL,McNeal MM,Cross TL,Greenberg HB

    更新日期:1993-11-01 00:00:00

  • Identification of a new group of bovine viral diarrhea virus strains associated with severe outbreaks and high mortalities.

    abstract::New BVDV strains associated with very high mortalities, which killed about 25% of the veal calves in Quebec in 1993, have been isolated. In this study, characterization of the last two-thirds of the 5' untranslated region (5'UTR) of their genome and virus neutralization experiments with polyvalent antisera raised in d...


    pub_type: 杂志文章


    authors: Pellerin C,van den Hurk J,Lecomte J,Tijssen P

    更新日期:1994-09-01 00:00:00

  • MMTV accessory factor Naf affects cellular gene expression.

    abstract::Mouse mammary tumour virus (MMTV) encodes a viral superantigen (Sag) and a negative acting factor (Naf) which share parts of their coding sequence. Using 2-dimensional gel electrophoresis (2D-DIGE), we could show that at least 10 different cellular proteins were differentially expressed in Naf positive cells. Also, lu...


    pub_type: 杂志文章


    authors: Metzner C,Salmons B,Gunzburg WH,Gemeiner M,Miller I,Gesslbauer B,Kungl A,Dangerfield JA

    更新日期:2006-03-01 00:00:00

  • Pathogenic prion protein fragment (PrP106-126) promotes human immunodeficiency virus type-1 infection in peripheral blood monocyte-derived macrophages.

    abstract::Transfusion of blood and blood products contaminated with the pathogenic form of prion protein Prp(sc), thought to be the causative agent of variant a Creutzfeldt-Jakob disease (vCJD), may result in serious consequences in recipients with a compromised immune system, for example, as seen in HIV-1 infection. In the pre...


    pub_type: 杂志文章


    authors: Bacot SM,Feldman GM,Yamada KM,Dhawan S

    更新日期:2015-02-01 00:00:00

  • Phosphorylated proteins in cauliflower mosaic virus.

    abstract::Autoradiography of dodecyl sulfate-impregnated polyacrylamide gels after electrophoresis of the proteins from 32P-labeled cauliflower mosaic virus (CaMV) showed that two of the structural proteins were phosphorylated. These two proteins of 44 and 58 kilodaltons (kd) occur as minor components with some strains of the v...


    pub_type: 杂志文章


    authors: Hahn P,Shepherd RJ

    更新日期:1980-11-01 00:00:00

  • Quantitative comparison of the HSV-1 and HSV-2 transcriptomes using DNA microarray analysis.

    abstract::The genomes of human herpes virus type-1 and type-2 share a high degree of sequence identity; yet, they exhibit important differences in pathology in their natural human host as well as in animal host and cell cultures. Here, we report the comparative analysis of the time and relative abundance profiles of the transcr...


    pub_type: 杂志文章


    authors: Aguilar JS,Devi-Rao GV,Rice MK,Sunabe J,Ghazal P,Wagner EK

    更新日期:2006-04-25 00:00:00

  • The carboxy-terminal domain is essential for stability and not for virion incorporation of HIV-1 Vpr into virus particles.

    abstract::Vpr is one of the auxiliary gene products encoded by HIV-1 genome. Vpr is a 14-kDa protein and exhibits several interesting characteristics including incorporation into virus particles, oligomerization, localization in the nucleus, and positive regulation of virus replication in primary cells. In an effort to define t...


    pub_type: 杂志文章


    authors: Mahalingam S,Patel M,Collman RG,Srinivasan A

    更新日期:1995-12-20 00:00:00

  • High level expression of nonfused foreign genes with Autographa californica nuclear polyhedrosis virus expression vectors.

    abstract::High levels of nonfused chloramphenicol acetyltransferase, beta-galactosidase, and beta-glucuronidase expressed under the control of new vector constructs of the polyhedrin promoter in Spodoptera frugiperda cells infected with Autographa californica nuclear polyhedrosis virus were investigated by SDS-PAGE and RNA dot ...


    pub_type: 杂志文章


    authors: Luckow VA,Summers MD

    更新日期:1989-05-01 00:00:00

  • Non-structural proteins and RNAs of alfalfa mosaic virus synthesized in tobacco and cowpea protoplasts.

    abstract::Three proteins, reacting specifically with sera raised against synthetic peptides identical to C-terminal amino acid sequences in alfalfa mosaic virus (AIMV) proteins P1, P2, and P3 translated in vitro from the AIMV RNAs 1, 2, and 3, respectively, were for the first time observed in tobacco and cowpea protoplasts. Par...


    pub_type: 杂志文章


    authors: van Pelt-Heerschap H,Verbeek H,Huisman MJ,Sue Loesch-Fries L,Van Vloten-Doting L

    更新日期:1987-11-01 00:00:00

  • Association of viral load with serum biomakers among COVID-19 cases.

    abstract::Since SARS-CoV-2 spreads rapidly around the world, data have been needed on the natural fluctuation of viral load and clinical indicators associated with it. We measured and compared viral loads of SARS-CoV-2 from pharyngeal swab, IgM anti-SARS-CoV-2, CRP and SAA from serum of 114 COVID-19 patients on admission. Posit...


    pub_type: 杂志文章


    authors: Shi F,Wu T,Zhu X,Ge Y,Zeng X,Chi Y,Du X,Zhu L,Zhu F,Zhu B,Cui L,Wu B

    更新日期:2020-07-01 00:00:00

  • Activity of vesicular stomatitis virus M protein mutants in cell rounding is correlated with the ability to inhibit host gene expression and is not correlated with virus assembly function.

    abstract::In addition to its role in virus assembly, the matrix (M) protein of vesicular stomatitis virus (VSV) is involved in virus-induced cell rounding and inhibition of host-directed gene expression. Previous experiments have shown that two M protein mutants genetically dissociate the ability of M protein to inhibit host-di...


    pub_type: 杂志文章


    authors: Lyles DS,McKenzie MO

    更新日期:1997-03-03 00:00:00

  • The HSV-1 2-kb latency-associated transcript is found in the cytoplasm comigrating with ribosomal subunits during productive infection.

    abstract::We have examined the nuclear and cytoplasmic distribution of the latency-associated transcripts (LATs) of HSV-1. During latency these transcripts accumulate in the nuclei of neurons in the peripheral and central nervous system of infected animals. However, our Northern blot analyses demonstrate that the 2-kb LAT is fo...


    pub_type: 杂志文章


    authors: Nicosia M,Zabolotny JM,Lirette RP,Fraser NW

    更新日期:1994-11-01 00:00:00

  • A non-consensus branch point plays an important role in determining the stability of the 2-kb LAT intron during acute and latent infections of herpes simplex virus type-1.

    abstract::Herpes simplex virus type 1 (HSV-1) establishes lifelong latent infection in sensory neurons of the peripheral nervous system. During HSV latency, the latency-associated transcripts (LATs) are the only viral transcripts abundantly expressed. The most abundant form of LATs is a 2-kb stable intron spliced from a primary...


    pub_type: 杂志文章


    authors: Mukerjee R,Kang W,Suri V,Fraser NW

    更新日期:2004-07-01 00:00:00

  • Pathogenesis of novel reassortant avian influenza virus A (H5N8) Isolates in the ferret.

    abstract::Outbreaks of avian influenza virus H5N8 first occurred in 2014, and spread to poultry farms in Korea. Although there was no report of human infection by this subtype, it has the potential to threaten human public health. Therefore, we evaluated the pathogenesis of H5N8 viruses in ferrets. Two representative Korean H5N...


    pub_type: 杂志文章


    authors: Kim HM,Kim CK,Lee NJ,Chu H,Kang C,Kim K,Lee JY

    更新日期:2015-07-01 00:00:00

  • Enterovirus infections of the central nervous system.

    abstract::Enteroviruses (EV) frequently infect the central nervous system (CNS) and induce neurological diseases. Although the CNS is composed of many different cell types, the spectrum of tropism for each EV is considerable. These viruses have the ability to completely shut down host translational machinery and are considered ...


    pub_type: 杂志文章,评审


    authors: Rhoades RE,Tabor-Godwin JM,Tsueng G,Feuer R

    更新日期:2011-03-15 00:00:00

  • MVM(p) NS-2 protein expression is required with NS-1 for maximal cytotoxicity in human transformed cells.

    abstract::The parvovirus-encoded nonstructural (NS) proteins have been implicated in the cytopathogenicity of these agents. Although protein NS-1 of minute virus of mice (MVM) has been shown to be toxic, little is known about the role of NS-2 in this process. In order to determine the contribution of NS-1 and NS-2 to cytotoxici...


    pub_type: 杂志文章


    authors: Legrand C,Rommelaere J,Caillet-Fauquet P

    更新日期:1993-07-01 00:00:00

  • Identification of proteins encoded by avian reoviruses and evidence for post-translational modification.

    abstract::Twelve avian reovirus strain 176 proteins, 10 structural (lambda 1, lambda 2, lambda 3, mu 1, mu 2/mu 2C, sigma 1, sigma 2, sigma 3, and sigma 4), and 2 nonstructural (mu NS and sigma NS) were identified and characterized by electrophoretic analysis of purified virions and infected cell lysates. Three of the identifie...


    pub_type: 杂志文章


    authors: Ni Y,Ramig RF,Kemp MC

    更新日期:1993-03-01 00:00:00