Abstract:
:The promotion of membrane fusion by the paramyxovirus hemagglutinin-neuraminidase (HN) and fusion (F) proteins requires that they be derived from homologous viruses, suggesting the possibility that the promotion of fusion requires a virus-specific communication between the two glycoprotein spikes. We have evaluated the ability of chimeric HN proteins, composed of domains from the HN proteins of two heterologous members of the group, human parainfluenza virus 3 (hPIV3) and Newcastle disease virus (NDV), to complement the F protein of each virus in the promotion of fusion. Specificity for the F protein of hPIV3 segregates with a segment composed of the transmembrane anchor and the first 82 residues of the ectodomain of its HN protein. Specificity of NDV HN for its homologous F protein is determined by a similar domain. These findings suggest that determinants specific to this segment of the attachment protein spike may be involved in the triggering of the fusion process.
journal_name
Virologyjournal_title
Virologyauthors
Deng R,Wang Z,Mirza AM,Iorio RMdoi
10.1006/viro.1995.1278subject
Has Abstractpub_date
1995-06-01 00:00:00pages
457-69issue
2eissn
0042-6822issn
1096-0341pii
S0042-6822(85)71278-0journal_volume
209pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::The major outer capsid protein, VP5, of five US bluetongue viruses, was found to be glycosylated. Enzymatic removal of the carbohydrate moiety did not affect the electrophoretic mobility of VP5 in SDS-PAGE, indicating the presence of only short and possibly unbranched oligosaccharide chains. The surface accessibilitie...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(83)90000-4
更新日期:1993-05-01 00:00:00
abstract::Multiplication of frog virus 3 (FV 3) occurs in mammalian cells provided they are incubated at temperatures lower than 33 degrees. The expression of the viral genome at supraoptimal temperatures was followed by analyzing the polypeptides produced in CHO-infected cells and comparing with those obtained under restrictiv...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90138-8
更新日期:1986-07-30 00:00:00
abstract::It has been demonstrated in a number of systems that CD8+ T cells (T CD8+) can be induced by peptides encoded in alternative reading frames (ARFs) that do not appear to code for bona fide proteins. The biological relevance of ARF peptides remains to be firmly established, however. With this as a goal, we systematicall...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2002.1744
更新日期:2003-01-05 00:00:00
abstract::The recently described taxon Negevirus is comprised of a diverse group of insect-specific viruses isolated from mosquitoes and phlebotomine sandflies. In this study, a comprehensive genetic characterization, molecular, epidemiological and evolutionary analyses were conducted on nearly full-length sequences of 91 new n...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2017.01.022
更新日期:2017-04-01 00:00:00
abstract::The poxvirus virion contains an inner tubular nucleocapsid structure. The nucleocapsid is apparently labile to conventional electron microscopy fixation procedures and has therefore been largely ignored for decades. Advancements in electron microscopy sample preparation, notably high pressure freezing, better preserve...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2012.08.019
更新日期:2012-12-05 00:00:00
abstract::Kaposi's sarcoma-associated herpesvirus origin-dependent DNA replication requires the core replication proteins plus K-Rta and K-bZIP. To determine which K-bZIP protein domains contribute to oriLyt-dependent DNA replication and facilitate suppression of K-Rta-mediated transcriptional activation, we generated a series ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2007.08.019
更新日期:2007-12-20 00:00:00
abstract::Peste des petits ruminants (PPR) is a viral disease of goats and sheep characterized by erosive stomatitis, enteritis, and pneumonia. The virus is a member of the family Paramyxoviridae and the genus Morbillivirus. The disease has high morbidity and mortality rates and has a substantial economic impact in developing c...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.1210
更新日期:1995-04-20 00:00:00
abstract::La Crosse (LAC) virions exposed to different pHs (7.3, 6.2, and 5.4) and temperatures (37 degrees, 20 degrees, and 4 degrees) were preserved in thin layers of vitreous ice and observed by electron cryomicroscopy. Our results indicate that, at lower pH, virus particles interact with each other to form aggregates. In so...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90869-d
更新日期:1991-09-01 00:00:00
abstract::Extensive cell killing and cytopathology were observed within 24 hr after exposure of a clonal cell line of human T-4 lymphocytes (RH9) to culture supernatants containing human immunodeficiency virus (HIV). Ultraviolet-irradiated HIV-containing culture fluids were also capable of killing RH9 cells and of inducing spec...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90465-4
更新日期:1986-10-30 00:00:00
abstract::The virion-associated Vpr protein of human immunodeficiency virus type 1 (HIV-1) alters cell cycle progression from the G2 phase, influences the virus in vivo mutation rate, and participates in the nuclear translocation of viral DNA. While many Vpr-interacting proteins have been identified, the functional relevance of...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2000.0791
更新日期:2001-03-30 00:00:00
abstract::Like human adenovirus type 5 (HAV5), bovine adenovirus type 3 (BAV3) early region 1 (E1) consists of E1A and E1B transcriptional units. In order to characterize BAV3 E1 proteins and to isolate a cell line of bovine origin that expresses BAV3 E1, polyclonal antibodies specific to E1A, E1B-157R, and E1B-420R were raised...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2002.1389
更新日期:2002-03-30 00:00:00
abstract::The genome of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) contains four structural genes that are homologous to genes found in other coronaviruses, and also contains six subgroup-specific open reading frames (ORFs). Expression of one of these subgroup-specific genes, ORF7a, resulted in apoptosi...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.10.015
更新日期:2006-03-01 00:00:00
abstract::The enveloped virions of two strains of a granulosis virus, a synergistic Hawaiian (GVH) and a nonsynergistic Oregonian (GVO) strain which infect the armyworm, Pseudaletia unipuncta, were liberated from their capsules with 0.02 N NaOH and purified by filtration through membrane filters. The envelopes were solubilized ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(79)90439-2
更新日期:1979-04-15 00:00:00
abstract::The Gardner (GA) and Snyder-Theilen (ST) isolates of feline sarcoma virus (FeSV) represent genetic recombinants between feline leukemia virus (FeLV) and transformation-specific sequences (v-fes gene) of cat cellular origin. A related transforming gene (v-fps), common to the Fujinami, PRC II, and UR 1 strains of avian ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(83)90219-2
更新日期:1983-03-01 00:00:00
abstract::Defective interfering particles of New Jersey serotype VSV were found to contain very little or no virion transcriptase activity. Furthermore no VSV transcriptase activity was observed in cells treated with cycloheximide and infected with DI. Cells infected with DI together with standard infectious virus and cyclohexi...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(72)90356-x
更新日期:1972-10-01 00:00:00
abstract::Three prophage sequences were identified in the Lactobacillus johnsoni strain NCC533. Prophage Lj965 predicted a gene map very similar to those of pac-site Streptococcus thermophilus phages over its DNA packaging and head and tail morphogenesis modules. Sequence similarity linked the putative DNA packaging and head mo...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2000.0498
更新日期:2000-09-30 00:00:00
abstract::Reactivation of latent herpes simplex virus 2 (HSV-2) infections can be characterized by episodic recurrent genital lesions and/or viral shedding. We hypothesize that infected (HSV-2(pos)) asymptomatic individuals have acquired T cell responses to specific HSV-2 antigen(s) that may be an important factor in controllin...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2014.07.018
更新日期:2014-09-01 00:00:00
abstract::Infection with bovine papillomavirus type 1 (BPV1) or BPV2 induces fibropapillomas in cows and skin sarcoids in horses. Prophylactic vaccination targeting BPV1 and BPV2 may reduce the incidence of these economically important diseases. The L1 major capsid proteins of BPV1 and BPV2 were expressed in Sf-9 insect cells a...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.07.036
更新日期:2009-10-10 00:00:00
abstract::A combinatorial, phage-display library of human Fab antibody fragments was generated from IgG heavy chain (HC) and light chain (LC) genes cloned from the lymphocytes of a vaccinia virus (VACV)-immune donor. To ascertain the complexity of the library, nucleotide sequences of the variable regions of the HC and LC genes ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1999.9701
更新日期:1999-05-25 00:00:00
abstract::The CC chemokine receptor (CCR) 7 ligands CCL21 and CCL19 were recently described as essential elements for establishing the microenvironment needed to initiate optimal immune responses in secondary lymphoid tissues. In the present study we have kinetically investigated the primary responses of naive DO11.10 TCR-trans...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(03)00199-5
更新日期:2003-07-20 00:00:00
abstract::Section of the sciatic nerve during the period of herpes simplex virus (HSV) latent infection was performed to evaluate residual latency in mouse dorsal root ganglion. In control mice without sciatic neurectomy, latency was present in 90-100%, while in those which underwent a neurectomy procedure, latent infection was...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90085-2
更新日期:1988-11-01 00:00:00
abstract::The 64K surface protein of budded Autographa californica nuclear polyhedrosis virus (AcMNPV BV) is known to play a role in the functional entry of AcMNPV BV into Spodoptera frugiperda IPLB-SF-21 cells by adsorptive endocytosis. AcV1, a neutralizing monoclonal antibody, reacts with the 64K protein and in doing so preve...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90176-6
更新日期:1988-09-01 00:00:00
abstract::Using a defective interfering Sendai virus stock (DIH4) freed of nondefective helper virus, we found that the closely related parainfluenza viruses 1 and 3 could substitute for the Sendai virus helper in replicating DIH4, creating chimeric nucleocapsids. The morbillivirus measles and the rhabdovirus VSV could not subs...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90660-4
更新日期:1991-05-01 00:00:00
abstract::The infectivity in vivo, replication competence in vitro, and expression of viral genes of several molecularly cloned duck hepatitis B virus (DHBV) genomes were investigated. In addition, replication competence, core protein expression, and secretion of viral proteins were investigated for a grey heron hepatitis B vir...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90782-7
更新日期:1991-11-01 00:00:00
abstract::The human papillomavirus type 16 E1--E4 protein is expressed abundantly in cells supporting viral DNA amplification, but its expression is lost during malignant progression. In cell culture, 16E1--E4 causes G2 cell cycle arrest by associating with and preventing the nuclear entry of Cdk1/cyclin B1 complexes. Here, we ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.02.024
更新日期:2006-05-25 00:00:00
abstract::Rift Valley fever virus-induced protein synthesis was examined by polyacrylamide gel electrophoresis and fluorography. Five virus-induced polypeptides were detected, the nucleocapsid protein N, the nucleus-associated nonstructural protein NS1, the glycoproteins G1 and G2, and a protein of molecular weight 80K. The N, ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90277-0
更新日期:1984-04-15 00:00:00
abstract::We show that the product of the polarity suppression (psu) gene from bacteriophage P4 associates with P4 capsids. This association can occur when Psu is (i) provided in vivo from the P4 genome or from a plasmid or (ii) provided in vitro by mixing viable phage particles with Psu protein. Psu is unable to associate with...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90538-z
更新日期:1992-06-01 00:00:00
abstract::The 5'-terminal noncoding region sequences were determined for the genome RNAs of seven strains of St. Louis encephalitis virus (SLEV) and one strain of West Nile virus (WNV) using a single synthetic cDNA primer complementary to the 5'-terminus of the coding region of a strain of WNV RNA. The 5'-terminal sequences obt...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90468-0
更新日期:1988-02-01 00:00:00
abstract::Our recent results demonstrated that bile acids facilitate virus escape from the endosomes into the cytoplasm for successful replication of porcine enteric calicivirus (PEC). We report a novel finding that bile acids can be substituted by cold treatment for endosomal escape and virus replication. This endosomal escape...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2015.04.022
更新日期:2015-09-01 00:00:00
abstract::We previously described a single-cycle dengue vaccine (RepliVAX D2) engineered from a capsid (C) gene-deleted West Nile virus (WNV) expressing dengue virus serotype 2 (DENV2) prM/E genes in place of the corresponding WNV genes. That work demonstrated that adaptation of RepliVAX D2 to grow in WNV C-expressing cells res...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2011.09.007
更新日期:2011-12-20 00:00:00