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Characterization of infectious and defective cloned avian hepadnavirus genomes.

Abstract：

:The infectivity in vivo, replication competence in vitro, and expression of viral genes of several molecularly cloned duck hepatitis B virus (DHBV) genomes were investigated. In addition, replication competence, core protein expression, and secretion of viral proteins were investigated for a grey heron hepatitis B virus genome. Except two, all DHBV isolates tested induced a systemic infection in Pekin ducks when injected as cloned viral DNA into the liver. After transfection of chicken hepatoma cells, both defective DHBV genomes expressed intracellular nucleocapsid and pre-S envelope proteins and secreted DHBs/pre-S particles into the medium. One of the defective DHBV genomes and HHBV produced within the cells replicative intermediates encapsidated in core particles and secreted virions, whereas the other defective DHBV genome did not and was unable to efficiently encapsidate the RNA pregenome. Comparative sequence analysis was performed to identify potential amino acid changes in viral proteins of both defective DHBV genomes. The data obtained demonstrate that most cloned avian hepadnaviruses are infectious or replication competent and suggest defects in envelope, polymerase or encapsidation function, respectively, in two cloned DHBV genomes.

journal_name

Virology

journal_title

Virology

authors

Wildner G,Fernholz D,Sprengel R,Schneider R,Will H

doi

10.1016/0042-6822(91)90782-7

subject

Has Abstract

pub_date

1991-11-01 00:00:00

pages

345-53

issue

1

eissn

0042-6822

issn

1096-0341

pii

0042-6822(91)90782-7

journal_volume

185

pub_type

杂志文章

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