Abstract:
:The terminal sequences of both genomic dsRNA segments of different strains of infectious bursal disease virus were determined. Analysis of the linear sequences revealed in the 5'-noncoding region of both segments the presence of a 32-nucleotide sequence with motifs conserved between segments and serotypes. The 3'-noncoding regions showed a high degree of conservation within segment A or B, but differed between segments. Different inverted terminal repeats were detected in both segments. A sequence of 13 nucleotides found in the 5'-noncoding regions of both segments could function as a binding site for chicken 18 S rRNA. Secondary structure prediction of the different noncoding regions showed a high degree of similarity. Surprisingly, the predicted secondary structure of the 5'-noncoding region of segment A differed between serotypes. A possible influence of these different RNA structures on viral replication is discussed.
journal_name
Virologyjournal_title
Virologyauthors
Mundt E,Müller Hdoi
10.1006/viro.1995.1226subject
Has Abstractpub_date
1995-05-10 00:00:00pages
10-8issue
1eissn
0042-6822issn
1096-0341pii
S0042-6822(85)71226-3journal_volume
209pub_type
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