Abstract:
:It has been shown that the genomes of episomally maintained DNA viruses are tethered to host cell chromosomes during cell division, facilitating maintenance in dividing cells. The papillomavirus E2 protein serves this mechanism of viral genome persistence by simultaneously associating with chromatin and the viral genome during mitosis. Several host cell proteins are reported to be necessary for the association of E2 with chromatin including the cohesion establishment factor ChlR1. Here we use fluorescence resonance energy transfer (FRET) technology to confirm the interaction between BPV-1 E2 and ChlR1. Furthermore, we use synchronised live cells to study the temporal nature of this dynamic protein interaction and show that ChlR1 and E2 interact during specific phases of the cell cycle. These data provide evidence that the association of E2 with ChlR1 contributes to a loading mechanism during DNA replication rather than direct tethering during mitotic division.
journal_name
Virologyjournal_title
Virologyauthors
Feeney KM,Saade A,Okrasa K,Parish JLdoi
10.1016/j.virol.2011.03.015subject
Has Abstractpub_date
2011-05-25 00:00:00pages
1-9issue
1eissn
0042-6822issn
1096-0341pii
S0042-6822(11)00140-1journal_volume
414pub_type
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