Abstract:
:The HSV-1 genome is composed of two unique regions (UL and US) flanked by inverted repeats. During the course of DNA replication the two unique regions UL and US invert relative to one another. In this report we present evidence that cleavage is not necessary for genomic inversion to occur. We isolated and characterized a UL6::lacZ insertion mutant (hr74) that produces wild-type levels of replicating viral DNA but fails to cleave and package DNA. We demonstrate that this virus is still able to undergo genomic inversion. Furthermore we confirm that replicating DNA from cells infected with wild-type virus contains specific UL terminal but not US termini, whereas cells infected with the mutant hr74 do not contain either US or UL termini. This demonstrates that the specific UL ends found in replicating DNA are the result of the cleavage/packaging process.
journal_name
Virologyjournal_title
Virologyauthors
Lamberti C,Weller SKdoi
10.1006/viro.1996.0668subject
Has Abstractpub_date
1996-12-15 00:00:00pages
403-7issue
2eissn
0042-6822issn
1096-0341pii
S0042-6822(96)90668-6journal_volume
226pub_type
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