Identification and characterization of a murine cytomegalovirus gene with homology to the UL25 open reading frame of human cytomegalovirus.

abstract：:We used a mouse nasal model of herpes simplex virus 2 (HSV-2) infection to examine the biological properties of HSV-2 wild-type (wt), TK-negative, and replication-defective strains in vivo. Nasal septa tissue is the major site of wt viral replication post intranasal (i.n.) inoculation. The HSV-2 strain 186 syn(+)-1 wt...

journal_title：Virology

authors： Jones CA,Taylor TJ,Knipe DM

更新日期：2000-12-05 00:00:00

abstract：:Trans-acting regulatory components of Autographa californica nuclear polyhedrosis virus (AcMNPV) were studied in a transient assay system for their ability to activate the p143 gene promoter. A region including 502 nt upstream of the AUG of the p143 gene was linked to the bacterial chloramphenicol acetyltransferase (C...

journal_title：Virology

authors： Lu A,Carstens EB

更新日期：1993-08-01 00:00:00

abstract：:Porcine circovirus 2 (PCV2) has a major impact on the swine industry. Eight PCV2 genotypes (a-h) have been identified using capsid sequence analysis. PCV2d has been designated as the emerging genotype. The cryo-electron microscopy molecular envelope of PCV2d virus-like particles identifies differences between PCV2a, b...

journal_title：Virology

authors： Khayat R,Wen K,Alimova A,Gavrilov B,Katz A,Galarza JM,Gottlieb P

更新日期：2019-11-01 00:00:00

abstract：:The coding region of segment 4 of Thogoto (THO) virus, a tick-borne member of the Orthomyxoviridae, was expressed in a baculovirus system under the control of the polyhedrin promoter. This construct expressed authentic envelope glycoprotein as determined by size and antigenic reactivity with a panel of monoclonal anti...

journal_title：Virology

authors： Jones LD,Morse MA,Marriott AC,Nuttall PA

更新日期：1995-10-20 00:00:00

abstract：:Tobacco (Nicotiana edwardsonii and N. benthamiana) protoplasts infected with the plant rhabdovirus sonchus yellow net virus (SYNV) were found to be suitable for studies of replication. SYNV messenger RNAs could be detected within 2 hr postinoculation (PI), accumulated to a maximum within 24 hr, and subsequently declin...

journal_title：Virology

authors： Jones RW,Jackson AO

更新日期：1990-12-01 00:00:00

abstract：:Cytoplasmic tails of envelope (Env) glycoproteins of many retroviruses inhibit their membrane fusion activity. The cytoplasmic 16-amino acid peptide of ecotropic murine leukemia virus (E-MLV) Env protein, called the R-peptide, also inhibits the membrane fusion activity of the Env protein. However, the molecular mechan...

journal_title：Virology

authors： Kubo Y,Izumida M,Togawa K,Zhang F,Hayashi H

更新日期：2019-06-01 00:00:00

abstract：:Marek's disease virus (MDV) is an oncogenic alphaherpesvirus of Gallus gallus, the domesticated chicken. Control strategies rely upon vaccination with live attenuated viruses of antigenically similar avian herpesviruses or attenuated strains of MDV. Recent studies in other viruses have shown that recoding certain vira...

journal_title：Virology

authors： Conrad SJ,Silva RF,Hearn CJ,Climans M,Dunn JR

更新日期：2018-03-01 00:00:00

abstract：:Viruses are infectious particles whose viability is dependent on the cells of living organisms, such as bacteria, plants, and animals. It is of great interest to discover how viruses function inside host cells in order to develop therapies to treat virally infected organisms. The fruit fly Drosophila melanogaster is a...

journal_title：Virology

authors： Hughes TT,Allen AL,Bardin JE,Christian MN,Daimon K,Dozier KD,Hansen CL,Holcomb LM,Ahlander J

更新日期：2012-02-05 00:00:00

abstract：:The expression of type I murine interferon (MuIFN) genes and several other cellular genes was examined in poly rI.rC induced and Newcastle disease virus (NDV) infected mouse cells. Northern analysis of RNA from induced L cells revealed that the MuIFN-alpha s are expressed efficiently in NDV infected cells but only at ...

journal_title：Virology

authors： Kelley KA,Pitha PM

更新日期：1985-12-01 00:00:00

abstract：:The polycation DEAE-dextran increased the binding of VSV to BHK cells about fourfold over a wide range of VSV concentrations. The same proportion of bound virions was internalized by the cell in the presence or absence of DEAE-dextran. Viral primary RNA production was increased to the equivalent of a 4- to 4.5-fold in...

journal_title：Virology

authors： Bailey CA,Miller DK,Lenard J

更新日期：1984-02-01 00:00:00

abstract：:The extraordinarily high level of genetic variation of HIV-1 env genes poses a challenge to obtain antibodies that cross-react with multiple subtype Env glycoproteins. To determine if cross-reactive monoclonal antibodies (mAbs) to highly conserved epitopes in HIV-1 envelope glycoproteins can be induced, we immunized m...

journal_title：Virology

authors： Gao F,Scearce RM,Alam SM,Hora B,Xia S,Hohm JE,Parks RJ,Ogburn DF,Tomaras GD,Park E,Lomas WE,Maino VC,Fiscus SA,Cohen MS,Moody MA,Hahn BH,Korber BT,Liao HX,Haynes BF

更新日期：2009-11-10 00:00:00

abstract：:The DNA sequence of the gag and pol regions of a provirus cloned from a bovine tumor is presented. In order to confirm these results the sequence of portions of a second clone, derived from a virus-producing cell line, was also determined. The gag gene was found to consist of 1179 nucleotides, which probably encode on...

journal_title：Virology

authors： Rice NR,Stephens RM,Burny A,Gilden RV

更新日期：1985-04-30 00:00:00

abstract：:The nucleotide and deduced amino acid sequence of G serotype 3 equine rotavirus strain H-2 was determined. A predicted 776-amino-acid H-2 VP4 shows less than or equal to 85.3% identity to other rotavirus VP4 types sequenced to date and thus represents a new P serotype. A PCR-generated probe derived from a cDNA clone o...

journal_title：Virology

authors： Hardy ME,Gorziglia M,Woode GN

更新日期：1993-03-01 00:00:00

abstract：:Synthesis of hepadnaviral DNA is dependent upon both the viral DNA polymerase and the viral core protein, the subunit of the nucleocapsids in which viral DNA synthesis takes place. In a study of natural isolates of duck hepatitis B virus (DHBV), we cloned full-length viral genomes from a puna teal. One of the clones f...

journal_title：Virology

authors： Guo H,Aldrich CE,Saputelli J,Xu C,Mason WS

更新日期：2006-09-30 00:00:00

abstract：:The Hepatitis B virus Precore protein, present in the secretory pathway as the HBeAg precursor, can associate in the cytoplasm with the Core protein to form heterocapsids, likely to favor viral persistence. Core and Precore proteins share their primary sequence except for ten additional aminoacids at the N-terminus of...

journal_title：Virology

authors： Duriez M,Thouard A,Bressanelli S,Rossignol JM,Sitterlin D

更新日期：2014-08-01 00:00:00

abstract：:HIV-1 transmission is usually initiated by a single viral strain called transmitted/ founder (T/F) virus. In in vitro models, HIV-1 can efficiently spread via cell-free and virological synapse (VS)-mediated cell-to-cell infection. Both modes of infection require the viral glycoprotein Envelope (Env). The efficiency wi...

journal_title：Virology

authors： Li H,Chen BK

更新日期：2019-01-02 00:00:00

abstract：:Wild-type vaccinia virus WR strain forms non-fusogenic (F-) large plaques and is hemagglutinin positive (HA+) under normal conditions of virus infection. We have analyzed a collection of spontaneous, highly attenuated mutants of vaccinia virus isolated from persistently infected Friend erythroleukemia cells (E. Paez, ...

journal_title：Virology

authors： Ortiz MA,Paez E

更新日期：1994-01-01 00:00:00

abstract：:The influenza A virus nucleoprotein previously expressed in Escherichia coli after fusion to 32 heterologous amino acids has now been purified and tested for its ability to form complexes with RNA in vitro. By using a simple filter binding assay, we show that ribonucleoprotein (RNP) complexes form readily with single-...

journal_title：Virology

authors： Kingsbury DW,Jones IM,Murti KG

更新日期：1987-02-01 00:00:00

abstract：:While the C-terminal domain of HIV-1 Vpu is critical for CD4 degradation, the transmembrane domain (TM) mediates ion channel activity, enhances virus release and is essential for counteracting CD317/Bst-2/Tetherin. Here we analyzed whether the ion channel activity of Vpu is required to antagonize CD317-mediated restri...

journal_title：Virology

authors： Bolduan S,Votteler J,Lodermeyer V,Greiner T,Koppensteiner H,Schindler M,Thiel G,Schubert U

更新日期：2011-07-20 00:00:00

abstract：:The human papillomavirus type 16 E1--E4 protein is expressed abundantly in cells supporting viral DNA amplification, but its expression is lost during malignant progression. In cell culture, 16E1--E4 causes G2 cell cycle arrest by associating with and preventing the nuclear entry of Cdk1/cyclin B1 complexes. Here, we ...

journal_title：Virology

authors： Davy CE,Ayub M,Jackson DJ,Das P,McIntosh P,Doorbar J

更新日期：2006-05-25 00:00:00

abstract：:The 121,752-bp genome sequence of bacteriophage T5 was determined; the linear, double-stranded DNA is nicked in one of the strands and has large direct terminal repeats of 10,139 bp (8.3%) at both ends. The genome structure is consistently arranged according to its lytic life cycle. Of the 168 potential open reading f...

journal_title：Virology

authors： Wang J,Jiang Y,Vincent M,Sun Y,Yu H,Wang J,Bao Q,Kong H,Hu S

更新日期：2005-02-05 00:00:00

abstract：:Enteroviruses (EV) frequently infect the central nervous system (CNS) and induce neurological diseases. Although the CNS is composed of many different cell types, the spectrum of tropism for each EV is considerable. These viruses have the ability to completely shut down host translational machinery and are considered ...

journal_title：Virology

authors： Rhoades RE,Tabor-Godwin JM,Tsueng G,Feuer R

更新日期：2011-03-15 00:00:00

abstract：:Missing contact footprinting with formic acid as a modifying reagent was used to examine specific IE-1 binding contacts to double-stranded oligonucleotides that contained either a consensus hr repeat sequence or a sequence from the pe38 promoter, which is down regulated by IE-1. The hr repeat sequences contain two con...

journal_title：Virology

authors： Leisy DJ,Rohrmann GF

更新日期：2000-08-15 00:00:00

abstract：:The mechanism of intracellular retention for the large surface protein (L) of duck hepatitis B virus (DHBV) was analyzed by examination of the transmembrane topologies and secretory properties of a collection of DHBV L mutants and compared with that of human hepatitis B virus (HBV) L. Our results demonstrate that, in ...

journal_title：Virology

authors： Gazina EV,Lin B,Gallina A,Milanesi G,Anderson DA

更新日期：1998-03-15 00:00:00

abstract：:We previously demonstrated in trials of a variety of experimental vaccines to equine infectious anemia virus (EIAV) a remarkable spectrum of efficacy ranging from sterilizing protection to severe enhancement of virus replication and disease, depending on the immunization strategy used. This range of vaccine efficacy o...

journal_title：Virology

authors： Hammond SA,Raabe ML,Issel CJ,Montelaro RC

更新日期：1999-09-30 00:00:00

abstract：:Cauliflower mosaic virus gene VI product (P6) is an essential protein that forms cytoplasmic, inclusion bodies (IBs). P6 contains four regions involved in self-association, termed D1-D4. D3 binds to D1, along with D4 and contains a spacer region (termed D3b) between two RNA-binding domains. Here we show D3b binds full...

journal_title：Virology

authors： Lutz L,Okenka G,Schoelz J,Leisner S

更新日期：2015-02-01 00:00:00

abstract：:We previously demonstrated that human parainfluenza virus type 2 (hPIV-2) induces RhoA activation, which promotes its growth. RhoA controls the equilibrium between globular and filamentous actin (F-actin). We found that F-actin formation is induced by wild type (wt) hPIV-2 infection, and that inhibition of F-actin for...

journal_title：Virology

authors： Ohta K,Matsumoto Y,Yumine N,Nishio M

更新日期：2018-11-01 00:00:00

abstract：:The virulence of herpes simplex virus-1 (HSV-1) strains by the intraperitoneal (ip) route of injection in mice depends on the presence of an intact sequence in the HpaI DNA fragment P within coordinates 0.762 to 0.787. Deletion of the HpaI-P region (e.g., strain HFEM) abrogates the ability of the virus to infect mice ...

journal_title：Virology

authors： Becker Y,Hadar J,Tabor E,Ben-Hur T,Raibstein I,Rösen A,Darai G

更新日期：1986-03-01 00:00:00

abstract：:This report presents a procedure for evaluating the relative affinities of simple sialoside receptor determinants for their interaction with the receptor binding pocket of the influenza virus hemagglutinin. The virus examined was A/Memphis/102/72, previously shown to exhibit preferential binding specificity for enzyma...

journal_title：Virology

authors： Pritchett TJ,Brossmer R,Rose U,Paulson JC

更新日期：1987-10-01 00:00:00

abstract：:Monoclonal antibodies were used to compare the antigenicities of the haemagglutinins of two receptor binding mutants of X-31 (H3N2) influenza virus. The mutants which differed from each other in recognizing sialic acid in either alpha 2-6 linkage or alpha 2-3 linkage to galactose also differed exclusively at residue 2...

journal_title：Virology

authors： Daniels RS,Douglas AR,Skehel JJ,Wiley DC,Naeve CW,Webster RG,Rogers GN,Paulson JC

更新日期：1984-10-15 00:00:00