Abstract:
:Tobacco (Nicotiana edwardsonii and N. benthamiana) protoplasts infected with the plant rhabdovirus sonchus yellow net virus (SYNV) were found to be suitable for studies of replication. SYNV messenger RNAs could be detected within 2 hr postinoculation (PI), accumulated to a maximum within 24 hr, and subsequently declined to undetectable levels by 60 hr. Plus- and minus-sense genomic RNAs appeared later and were most abundant by 36 hr PI, but the levels decreased by 60 hr. The four major SYNV structural proteins could be detected by Western blot serological analyses by 24 hr PI and were present in highest concentrations between 43 and 60 hr PI. Among various glycosylation inhibitors, only tunicamycin treatment of protoplasts altered viral protein patterns and resulted in synthesis of a glycoprotein with an apparent molecular weight (Mr) 10% smaller than that found in untreated protoplasts. Two specific cleavage products of the nucleocapsid protein estimated to be 21,000 and 37,000 Mr appeared in N. benthamiana-infected protoplasts by 60 hr PI, but in the presence of tunicamycin, the cleavage products were evident by 38 hr. This result suggests that specific cleavage of the N protein is correlated with stress of infected cells due to viral accumulation and/or tunicamycin treatment.
journal_name
Virologyjournal_title
Virologyauthors
Jones RW,Jackson AOdoi
10.1016/0042-6822(90)90149-lsubject
Has Abstractpub_date
1990-12-01 00:00:00pages
815-20issue
2eissn
0042-6822issn
1096-0341journal_volume
179pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::Granulin and polyhedrin proteins were purified by preparative sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis from the baculoviruses Autographa californica, Rachiplusia ou, Heliothis zea, Heliothis armigera, Trichoplusia ni, and Spodoptera frugiperda. Antisera were raised against Autographa californica...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(80)90128-2
更新日期:1980-05-01 00:00:00
abstract::In genetically susceptible C57BL/6 mice the LP-BM5 isolate of murine retroviruses causes profound splenomegaly, lymphadenopathy, hypergammaglobulinemia, and an immunodeficiency syndrome bearing many similarities to the pathologies seen in AIDS. Because of these similarities, which also include terminal B cell lymphoma...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8970
更新日期:1998-02-15 00:00:00
abstract::A combination of linker scanning mutagenesis and deletional analyses has been used to determine the role of individual DNA-protein binding sites on expression from the hepatitis B virus (HBV) enhancer I-X promoter (map position (mp) 1042-1354, HBV adw2). Linker scanning mutation of the EF-C site caused a 67.5% drop in...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1173
更新日期:1993-04-01 00:00:00
abstract::Open reading frame 89 (ORF89) is one of the three genes that are believed to be involved in the latent infection of white spot syndrome virus (WSSV). Here, we report the structure and functional characterization of ORF89. cDNA sequencing, 5' RLM-RACE, and 3' RLM-RACE showed that ORF89 gene is transcribed into an unspl...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2004.05.005
更新日期:2004-07-20 00:00:00
abstract::DNA immunization in conjunction with antiretroviral therapy was evaluated in SIV-infected rhesus macaques treated with [R]-9-[2-phosphonylmethoxypropyl]adenine (PMPA). Macaques were immunized monthly with DNA vaccines expressing either SIV gag/tat or SIV gag/tat and 19 CD8+ T cell epitopes during 7 months of therapy. ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.12.008
更新日期:2006-04-25 00:00:00
abstract::We recently developed an in vitro transcapsidation system in which infectivity of single-shelled (ss) rotavirus particles was successfully rescued (Chen and Ramig, Virology [1993]). Here, we report the rescue of infectivity of rotavirus core particles using virus strain B223 (G serotype 10) as the core donor and strai...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1315
更新日期:1993-06-01 00:00:00
abstract::Sulfolobus turreted icosahedral virus (STIV) infects Sulfolobus species found in the hot springs of Yellowstone National Park. Its 37 open reading frames (ORFs) generally lack sequence similarity to other genes. One exception, however, is ORF B116. While its function is unknown, orthologs are found in three additional...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2007.01.027
更新日期:2007-07-05 00:00:00
abstract::Herpes simplex virus 1 (HSV-1) glycoprotein B (gB) is a multifunctional glycoprotein required for infectivity; it is thought to promote fusion of the viral envelope with the cell membrane and entry of virions into cells. To map the antigenic and functional domains on gB, we constructed amino terminal derivatives lacki...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90102-5
更新日期:1989-09-01 00:00:00
abstract::We have examined the synthesis of the T protein of the human polyomavirus, JCV, during productive infection in primary cultures of human fetal glial and kidney cells. Immunoprecipitation of protein extracts from virus infected cells revealed that the JCV large T protein from both the prototype Mad and HEK adapted stra...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90418-6
更新日期:1986-01-15 00:00:00
abstract::Neutralizing monoclonal antibodies 2F1 and 1C10, which are specific for VP7 of serotype 2 rotaviruses (G2), were used to select neutralization escape variants of the human serotype 2 rotavirus, DS-1. Neutralization survival patterns generated by monoclonal antibodies 2F1, 1C10, and RV5:3 indicated that 2F1 and 1C10 di...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1601
更新日期:1993-11-01 00:00:00
abstract::A novel human papillomavirus type (HPV) was cloned from an invasive cervical carcinoma. The viral clone showed no homology with other known prototypes of HPV (HPV-1 through HPV-57), except HPV-33 by Southern blot analysis under stringent conditions. It showed less than 20% homology to HPV-33 by reassociation kinetic a...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90560-e
更新日期:1990-08-01 00:00:00
abstract::The SARS-coronavirus (SARS-CoV) is the etiological agent of the severe acute respiratory syndrome (SARS). The SARS-CoV spike (S) glycoprotein mediates membrane fusion events during virus entry and virus-induced cell-to-cell fusion. The cytoplasmic portion of the S glycoprotein contains four cysteine-rich amino acid cl...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.10.034
更新日期:2007-04-10 00:00:00
abstract::A chloroplast-localized papaya methionine sulfoxide reductase B1 (PaMsrB1) interacting with Papaya ringspot virus (PRSV) NIa-Pro was identified using a Sos recruitment two-hybrid system (SRS). SRS analysis of several deletion mutants of PRSV NIa-Pro and PaMsrB1 demonstrated that the C-terminal (residues 133-239) fragm...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2012.09.007
更新日期:2012-12-05 00:00:00
abstract::The complete genome sequence of the Liaoning isolate of the Antheraea pernyi multinucleocapsid nucleopolyhedrovirus (AnpeMNPV) was determined. The viral genome size is 126,246 bp, relatively GC-rich (53.5% G+C), with 145 predicted open reading frames (ORFs) of more than 50 amino acids, accounting for more than 97% of ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2007.04.027
更新日期:2007-09-30 00:00:00
abstract::LP-BM5, a retroviral isolate, induces a disease featuring an acquired immunodeficiency syndrome termed murine AIDS (MAIDS). Many of the features of the LP-BM5-initiated disease are shared with HIV/AIDS. Our lab has shown that the interaction of B and CD4 T cells that is central to MAIDS pathogenesis requires ligation ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.10.002
更新日期:2007-03-30 00:00:00
abstract::Expansion of CHIKV outbreaks with appearance of novel mutations are reported from many parts of the world. Two novel mutations viz. E1:K211E and E2:V264A in background of E1:226A are recently identified from Aedes aegypti dominated areas of India. In this study, the role of these mutations in modulation of infectivity...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2016.06.025
更新日期:2016-10-01 00:00:00
abstract::Although human polyomavirus JC (JCV) is known to cause progressive multifocal leukoencephalopathy (PML) in immunocompromised individuals, the mechanism by which JCV crosses the blood-brain barrier (BBB) remains unclear. To test our hypothesis that cell-free JCV gains entry into the brain by infecting endothelial cells...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2007.02.018
更新日期:2007-07-20 00:00:00
abstract::The recently described taxon Negevirus is comprised of a diverse group of insect-specific viruses isolated from mosquitoes and phlebotomine sandflies. In this study, a comprehensive genetic characterization, molecular, epidemiological and evolutionary analyses were conducted on nearly full-length sequences of 91 new n...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2017.01.022
更新日期:2017-04-01 00:00:00
abstract::cDNAs of beet necrotic yellow vein virus RNAs 3 and 4 could be rendered biologically active when they were placed under the control of the cauliflower mosaic virus 35S promoter and polyadenylation signal. Although the 35S in vivo transcripts should have contained up to forty 5' and several hundred 3' nonviral nucleoti...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90806-m
更新日期:1991-11-01 00:00:00
abstract::We described a simple method to introduce site-specific mutations into region E3 of adenovirus (Ad). Mutations are made in cloned Ad2 EcoRI-D (map position 76-83), then ligated between Ad5 EcoRI-A (map position 0-76) and EcoRI-B (map position 83-100) to complete the viral genome. We have used this method to isolate a ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90412-5
更新日期:1986-01-15 00:00:00
abstract::Human gammaherpesvirus 8 (HHV-8) is the etiologic agent of Kaposi's sarcoma, multicentric Castleman's disease and primary effusion lymphoma. Like other herpesviruses, the HHV-8 may exhibit latent or lytic cycle, both regulated by viral and host factors. Regarding host factors, we analysed the association of polymorphi...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2019.07.011
更新日期:2019-09-01 00:00:00
abstract::Trichomonas vaginalis viruses (TVV), which may regulate P270 gene expression in the protozoan pathogen T. vaginalis, are a group of divergent double-stranded (ds) RNA viruses. In the present study, the complete 4674-bp cDNA sequence of a 4.6-kb ds RNA from a newly identified TVV2-1 isolate was determined. The sequence...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1999.0129
更新日期:2000-02-15 00:00:00
abstract::Viruses are infectious particles whose viability is dependent on the cells of living organisms, such as bacteria, plants, and animals. It is of great interest to discover how viruses function inside host cells in order to develop therapies to treat virally infected organisms. The fruit fly Drosophila melanogaster is a...
journal_title:Virology
pub_type: 杂志文章,评审
doi:10.1016/j.virol.2011.11.016
更新日期:2012-02-05 00:00:00
abstract::Genome-linked protein VPg of Potato virus A (PVA; genus Potyvirus) has essential functions in all critical steps of PVA infection, i.e. replication, movement, and virulence. Structural features of the recombinant PVA VPg were investigated with the aim to create an outline for structure-function relationships. Circular...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.04.025
更新日期:2008-08-01 00:00:00
abstract::There is growing evidence that the receptor-binding characteristics of influenza viruses are affected by the host-dependent glycosylation of viral hemagglutinin (HA). To better understand these effects, we propagated two variants of the human influenza virus USSR/90/77 (which differed by the mutation Asn131 reversible...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9224
更新日期:1998-08-01 00:00:00
abstract::The role of binding kinetics in determining neutralizing potency for antiviral antibodies is poorly understood. While it is believed that increased steady-state affinity correlates positively with increased virus-neutralizing activity, the relationship between association or dissociation rate and neutralization potenc...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2014.02.010
更新日期:2014-04-01 00:00:00
abstract::The genes required for replication of the temperate bacteriophage P4, which are coded by the phage left operon, are expressed from a constitutive promoter (PLE). In the lysogenic state, repression of the P4 replication genes is achieved by premature transcription termination. The leader region of the left operon encod...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0455
更新日期:1996-09-01 00:00:00
abstract::We previously demonstrated that cellular mRNAs are degraded in CD4 positive lymphocytes infected by the human immunodeficiency virus, HIV-1, but not in cells infected by the simian lentivirus, SIV. To begin to define the molecular mechanisms underlying this RNA degradation, we have established an in vitro RNA degradat...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0103
更新日期:1996-03-01 00:00:00
abstract::The location of the cistron encoding the genome-linked protein (VPg) in the potyvirus tobacco vein mottling virus (TVMV) was investigated. Precipitation of 125I-labeled VPg with anti-tobacco etch virus 49K nuclear inclusion protein antiserum (which reacts with the NIa nuclear inclusion protein of TVMV) indicated that ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90307-8
更新日期:1988-04-01 00:00:00
abstract::DNA obtained from New Zealand white rabbit oral papillomas was analyzed for the presence of papillomavirus DNA. The viral genome was cloned as three separate subclones, which were each mapped and oriented with respect to one another. Comparisons with other papillomavirus DNAs by Southern blot hybridization under vario...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90411-4
更新日期:1988-01-01 00:00:00