Abstract:
:Although human polyomavirus JC (JCV) is known to cause progressive multifocal leukoencephalopathy (PML) in immunocompromised individuals, the mechanism by which JCV crosses the blood-brain barrier (BBB) remains unclear. To test our hypothesis that cell-free JCV gains entry into the brain by infecting endothelial cells, we inoculated human brain microvascular endothelial (HBMVE) cells with 50 HAU (1.33+/-0.27 x 10(7) genome copies) of JCV(Mad1) and analyzed the expression of early and late viral genes and proteins by immunocytochemistry, quantitative real-time PCR (qPCR), quantitative real-time reverse transcriptase PCR (qRT-PCR) and immunoprecipitation followed by Western blotting. JCV infected and replicated efficiently in HBMVE cells and produced infectious virions several hundred fold higher than the infecting inoculum. HBMVE cells in vitro did not express serotonin receptor 2A (5HT(2A)R), and 5HT(2A)R blockers did not prevent JCV infection of HBMVE cells. Collectively, our data indicate that the productive in vitro infection of HBMVE cells by JCV is independent of 5HT(2A)R.
journal_name
Virologyjournal_title
Virologyauthors
Chapagain ML,Verma S,Mercier F,Yanagihara R,Nerurkar VRdoi
10.1016/j.virol.2007.02.018subject
Has Abstractpub_date
2007-07-20 00:00:00pages
55-63issue
1eissn
0042-6822issn
1096-0341pii
S0042-6822(07)00113-4journal_volume
364pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::The genetic diversity of group M HIV-1 is highest in west central Africa. Blood samples from four locations in Cameroon were collected to determine the molecular epidemiology of HIV-1. The C2-V5 region of envelope was sequenced from 39 of the 40 samples collected, and 7 samples were sequenced across the genome. All st...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2001.0976
更新日期:2001-07-20 00:00:00
abstract::Studies of the cellular autophagy pathway have exploded over the past twenty years. Now appreciated as a constitutive degradative mechanism that promotes cellular homeostasis, autophagy is also required for a variety of developmental processes, cellular stress responses, and immune pathways. Autophagy certainly acts a...
journal_title:Virology
pub_type: 杂志文章,评审
doi:10.1016/j.virol.2015.03.042
更新日期:2015-05-01 00:00:00
abstract::Human enteric adenovirus Ad41 is associated with children gastroenteritis. To infect gastrointestinal cells, the invading virus must be acid-stable and resistant to inactivation by bile salts and proteases. In addition, it has to cross the mucus barrier before it infects mucosa cells. We show that Ad41 infectivity is ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2004.01.020
更新日期:2004-04-25 00:00:00
abstract::Rotavirus (RV) is the leading cause of infantile gastroenteritis worldwide. RV nonstructural protein 4 (NSP4), the first characterized viral enterotoxin, is a 28-kDa glycoprotein that has pleiotropic functions in RV infection and pathogenesis. NSP4 has multiple forms enabling it to perform its different functions. Dis...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2007.11.021
更新日期:2008-03-30 00:00:00
abstract::Varicella Zoster virus (VZV) productively infects humans causing varicella upon primary infection and herpes zoster upon reactivation from latency in neurons. In vitro studies using cell-associated VZV infection have demonstrated productive VZV-infection, while a few recent studies of human neurons derived from stem c...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2013.05.021
更新日期:2013-09-01 00:00:00
abstract::The requirement for the herpes simplex virus type 1 (HSV-1) protein Vmw65 (VP16) for activation of immediate early (IE) gene expression was examined in synchronized HeLa cells. Analyses of IE RNA levels were conducted during infection with a viral Vmw65 mutant, in1814. The results revealed an increased requirement for...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90695-l
更新日期:1992-07-01 00:00:00
abstract::The mature flavivirus capsid protein (virion C) is commonly thought to be free in the cytoplasm of infected cells and to form a nucleocapsid-like complex with genomic RNA in mature virus particles. There is little sequence conservation among flavivirus virion C proteins, but they are similar in size (e.g., 99 amino ac...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8608
更新日期:1997-06-23 00:00:00
abstract::West Nile virus (WNV) has become firmly established in northeastern US, reemerging every summer since its introduction into North America in 1999. To determine whether WNV overwinters locally or is reseeded annually, we examined the patterns of viral lineage persistence and replacement in Connecticut over 10 consecuti...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2011.06.006
更新日期:2011-08-15 00:00:00
abstract::It has previously been shown that a far-upstream region of the herpes simplex virus immediate early (IE) gene 3 increases the expression of a linked thymidine kinase (TK) gene and also contains sequences which respond to stimulation of transcription by a virion component. The effects of altering the orientation and di...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90238-1
更新日期:1984-09-01 00:00:00
abstract::In human immunodeficiency virus type 1 (HIV-1) and simian immunodeficiency virus (SIV) the gag gene encodes the precursor polyprotein Pr55Gag, which is cleaved by the viral protease to produce the major structural proteins. Recently, it has been shown that HIV and SIV gag RNAs contain internal ribosome entry sites (IR...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.10.003
更新日期:2007-03-30 00:00:00
abstract::In genetically susceptible C57BL/6 mice the LP-BM5 isolate of murine retroviruses causes profound splenomegaly, lymphadenopathy, hypergammaglobulinemia, and an immunodeficiency syndrome bearing many similarities to the pathologies seen in AIDS. Because of these similarities, which also include terminal B cell lymphoma...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8970
更新日期:1998-02-15 00:00:00
abstract::Human Adenovirus Type 4 (HAdV-4) is responsible for epidemic outbreaks of Acute Respiratory Disease (especially in military recruits), and is known to cause significant morbidity with several reported cases of mortality. However, we do not understand why this serotype causes such high morbidity, and have little insigh...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.01.017
更新日期:2008-05-10 00:00:00
abstract::Binding of a specific peptide(s) from a viral protein to major histocompatibility complex (MHC) class I molecules is a critical step in the activation of CD8(+) cytotoxic T lymphocytes (CTLs). Once activated, CTLs can cause lethal disease in an infected host, for example, by killing virus-containing ependymal and vent...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9593
更新日期:1999-04-10 00:00:00
abstract::The replicative form (RF) of spiroplasma virus 4 (SpV4) has been purified from infected cells of Spiroplasma melliferum strain G1 by alkaline lysis followed by low melting point agarose gel electrophoresis. A partial restriction map has been established. The circular RF was linearized by cutting at the unique ClaI res...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90060-7
更新日期:1986-06-01 00:00:00
abstract::An emv-14-derived, replication-competent ecotropic murine leukemia virus [MuLV], designated AK7, was previously cloned from the AKXL-5 recombinant inbred mouse strain and partially characterized. While genetically encoding for an envelope-derived immunodominant CTL epitope [KSPWFTTL] located in the transmembrane regio...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8747
更新日期:1997-09-29 00:00:00
abstract::In order to establish whether the poor growth of the two fastidious adenoviruses types 40 and 41 (Ad40 and Ad41) in HeLa cells is due to a reduced trans-activation by the early region 1A (E1A), we have determined the trans-activating effect of this region on the expression of the chloramphenicol acetyltransferase (CAT...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(87)90080-8
更新日期:1987-09-01 00:00:00
abstract::Studies were performed to induce cross-clade neutralizing antibodies (Abs) by testing various combinations of prime and boost constructs that focus the immune response on structurally-conserved epitopes in the V3 loop of HIV-1 gp120. Rabbits were immunized with gp120 DNA containing a V3 loop characterized by the GPGR ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.05.039
更新日期:2009-09-15 00:00:00
abstract::Human papillomavirus (HPV) E6 oncoproteins target numerous cellular proteins for ubiquitin-mediated degradation. In the case of p53 this is mediated by the E6AP ubiquitin ligase. However, there are conflicting reports concerning how central E6AP is to the global function of the HPV-16 and HPV-18 E6 oncoproteins. To in...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.07.029
更新日期:2009-10-10 00:00:00
abstract::Transfusion of blood and blood products contaminated with the pathogenic form of prion protein Prp(sc), thought to be the causative agent of variant a Creutzfeldt-Jakob disease (vCJD), may result in serious consequences in recipients with a compromised immune system, for example, as seen in HIV-1 infection. In the pre...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2014.11.032
更新日期:2015-02-01 00:00:00
abstract::We analyzed various mutants of tomato leaf curl virus-India to investigate the role of ORFs AV3, AV2, and coat protein (CP) in viral replication, movement, and symptom development. The results of these studies indicate that ORF AV3 does not encode a protein. Plants inoculated with infectious DNA which contained deleti...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0546
更新日期:1996-10-15 00:00:00
abstract::Felis catus papillomavirus type 2 (FcaPV2) DNA is found in feline cutaneous squamous cell carcinomas (SCCs); however, its biological properties are still uncharacterized. In this study, we successfully expressed FcaPV2 E6 and E7 putative oncogenes in feline epithelial cells and demonstrated that FcaPV2 E6 binds to p53...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2016.05.017
更新日期:2016-09-01 00:00:00
abstract::The open reading frame EP153R, located within the EcoRI E' fragment of the African swine fever (ASF) virus genome, is predicted to encode a membrane protein of 153 amino acids that presents significant homology to the N-terminal region of several CD44 molecules. EP153R contains multiple putative sites for N-glycosylat...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1999.0080
更新日期:2000-01-20 00:00:00
abstract::Immunodeficiency caused by HIV infection probably results from profound dysregulation of normal T lymphocyte properties by the virus. Despite description of the virus cytopathicity and numerous modifications in T cell functions, such as perturbation of antigen receptor signaling, CD4 downregulation, and induction of a...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1124
更新日期:1994-03-01 00:00:00
abstract::Antiviral cytotoxic T-cells are critical for control of lymphocytic choriomeningitis virus (LCMV) infection in mice. In H-2b mice, the antiviral response is directed against three Db-restricted epitopes in the viral nucleoprotein (NP396-404) and glycoprotein (GP276-286 and GP33-41). Our present data revealed a clear h...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8934
更新日期:1998-01-05 00:00:00
abstract::A procedure was developed for the purification of the pea enation mosaic virus (PEMV) using differential and rate zonal centrifugation in sucrose density gradients. Infectivity and serological data indicated that the highest virus concentration occurs in infected peas (Pisum sativum) 10-12 days after mechanical inocul...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(66)90060-2
更新日期:1966-03-01 00:00:00
abstract::Glycoprotein C (gC) of pseudorabies virus, a swine herpesvirus, initiates virus attachment by binding to heparan sulfate (HS) linked to proteoglycans (HSPGs) on the cell surface. This interaction facilitates a required step in virus entry, the binding to a non-HS coreceptor, likely by another viral glycoprotein, gD. W...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(02)00024-7
更新日期:2003-03-01 00:00:00
abstract::The genome of the lytic Pseudomonas putida bacteriophage gh-1 is linear double-stranded DNA containing 37,359 bp with 216-bp direct terminal repeats. Like other members of the T7 group, the gh-1 genome contains regions of high homology to T7 interspersed with nonhomologous regions that contain small open reading frame...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(03)00124-7
更新日期:2003-07-05 00:00:00
abstract::The regions of the AcMNPV genome represented as cytoplasmic transcripts early after infection of Spodoptera frugiperda cells prior to and encompassing the initiation of DNA replication were mapped. In vivo(32)P pulse-labeled cytoplasmic RNA from infected cells at various times postinfection was used to probe Southern ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(83)90491-9
更新日期:1983-04-15 00:00:00
abstract::The N-terminal portion of the reovirus cell attachment protein sigma 1 has recently been shown to possess intrinsic oligomerization and virion-anchoring functions. Sequence analysis of the sigma 1 proteins of the three reovirus serotypes has revealed the presence of distinct structural domains within this region: a te...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90678-5
更新日期:1991-05-01 00:00:00
abstract::By the use of reverse transcription followed by polymerase chain reaction (RT-PCR), we have identified one shorter than full-length, pregenomic viral RNA species in liver samples of woodchucks chronically infected with the woodchuck hepatitis virus (WHV). The spliced WHV RNA of about 2.4 kb in length was cloned and pa...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)91205-9
更新日期:1992-09-01 00:00:00