Abstract:
:Granulin and polyhedrin proteins were purified by preparative sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis from the baculoviruses Autographa californica, Rachiplusia ou, Heliothis zea, Heliothis armigera, Trichoplusia ni, and Spodoptera frugiperda. Antisera were raised against Autographa californica (Ac) polyhedrin and Trichoplusia ni (Tn) granulin and analyzed for homologous and heterologous immunoreactivity by immunodiffusion and radioimmunoassay (RIA). Ac polyhedrin and Tn granulin antisera recognized antigenic determinants on several baculovirus polyhedrin and granulin proteins even though the heterologous proteins had different immunoreactivities when compared by competition radioimmunoassay. Antigenic differences among granulin and polyhedrin proteins were also detected by altered slopes of the competition reaction curves. Antiserum raised against Ac polyhedrin which was purified in the presence of SDS was tested by competition RIA for its ability to detect and react with native polyhedrin produced in the infected TN-368 cells. Ac polyhedrin antiserum had similar if not identical ability to bind to native polyhedrin and to polyhedrin purified in the presence of SDS.
journal_name
Virologyjournal_title
Virologyauthors
Summers MD,Hoops Pdoi
10.1016/0042-6822(80)90128-2subject
Has Abstractpub_date
1980-05-01 00:00:00pages
89-98issue
1eissn
0042-6822issn
1096-0341pii
0042-6822(80)90128-2journal_volume
103pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::The aim of this study was to establish a method that could differentiate antibodies against live and inactivated vaccines of porcine reproductive and respiratory syndrome virus (PRRSV). A blocking ELISA (b-ELISA) was established using the PRRSV non-structural protein, Nsp9, as the antigen and a monoclonal antibody, 2D...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2013.06.027
更新日期:2013-09-01 00:00:00
abstract::The reactivates of human sera with uninduced and phorbol ester (TPA)-induced human herpesvirus-8 (HHV-8)-infected BCBL-1 cells were examined by immunofluorescence assay (IFA) and by radioimmunoprecipitation reactions (RIP). The seroprevalence of HHV-8 infections is low in the United States general population and only ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9055
更新日期:1998-03-30 00:00:00
abstract::The Hepatitis B virus Precore protein, present in the secretory pathway as the HBeAg precursor, can associate in the cytoplasm with the Core protein to form heterocapsids, likely to favor viral persistence. Core and Precore proteins share their primary sequence except for ten additional aminoacids at the N-terminus of...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2014.06.013
更新日期:2014-08-01 00:00:00
abstract::Previously our laboratory constructed an HIV-1 which stably maintained a primer binding site (PBS) complementary to tRNA(His) by mutating the region of the provirus within U5 postulated to interact with the anticodon of tRNA(His) (J. Wakefield, S-M Kang, and C. D. Morrow, 1996, J. Virol, 70, 966-975). From the analysi...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8837
更新日期:1997-11-24 00:00:00
abstract::Maturation of the arenavirus GP precursor (GPC) involves proteolytic processing by cellular signal peptidase and the proprotein convertase subtilisin kexin isozyme 1 (SKI-1)/site 1 protease (S1P), yielding a tripartite complex comprised of a stable signal peptide (SSP), the receptor-binding GP1, and the fusion-active ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2012.10.038
更新日期:2013-02-05 00:00:00
abstract::The regions of the AcMNPV genome represented as cytoplasmic transcripts early after infection of Spodoptera frugiperda cells prior to and encompassing the initiation of DNA replication were mapped. In vivo(32)P pulse-labeled cytoplasmic RNA from infected cells at various times postinfection was used to probe Southern ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(83)90491-9
更新日期:1983-04-15 00:00:00
abstract::During respiratory syncytial virus (RSV) infection there is a close physical interaction between the filamentous actin (F-actin) and the virus, involving both inclusion bodies and the virus filaments. This interaction appears to occur relatively early in the replication cycle, and can be detected from 8 h post-infecti...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2007.08.007
更新日期:2007-12-20 00:00:00
abstract::Herpesvirus glycoprotein C (gC) functions as a major virus attachment protein. The gC sequence of the neurovirulent bovine herpesvirus type 5 (BHV-5) virus was determined and compared with the gC sequence of the nonneurovirulent BHV-1. Alignment of the predicted amino acid sequences of BHV-1 and BHV-5 gC ORFs showed t...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(95)80137-5
更新日期:1995-11-10 00:00:00
abstract::Sixteen overlapping fragments of the dengue-2 virus envelope (E) protein, expressed as trpE-E fusion products in Escherichia coli, were used to map the epitopes defined by a panel of 20 monoclonal antibodies (MAbs) by immunoblotting. Using this technique, the amino acid sequence of six antigenic domains on the E prote...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90450-4
更新日期:1992-04-01 00:00:00
abstract::A retroviral element (MSRV) defining a family of genetically inherited endogenous retroviruses (HERV-W) has recently been characterized in cell cultures from patients with multiple sclerosis (MS). To address the possible relationship with MS, direct detection of circulating virion RNA was proposed but revealed technic...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2001.1045
更新日期:2001-09-01 00:00:00
abstract::Cells transformed with specific regions of the foot-and-mouth disease virus (FMDV) genome have been constructed and analyzed with respect to viability and susceptibility to FMDV infection. Constitutive expression of an active protease 3C under the control of the tk promoter has been documented by the ability of transf...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.1459
更新日期:1995-09-10 00:00:00
abstract::When effector CD4+ T cells carrying integrated HIV-1 proviruses revert back to a resting memory state, the virus can remain silent in those cells for years. Following re-exposure to the nominal antigen or in response to other stimuli (e.g. pro-inflammatory cytokines), these cells can begin to produce virus. Here we de...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.04.019
更新日期:2009-06-20 00:00:00
abstract::Adenovirus inundates the productively infected cell with linear, double-stranded DNA and an abundance of single-stranded DNA. The cellular response to this stimulus is antagonized by the adenoviral E1B and E4 early genes. A mutant group C adenovirus that fails to express the E1B-55K and E4orf3 genes is unable to suppr...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2014.03.010
更新日期:2014-05-01 00:00:00
abstract::In 2014-2015, the U.S. experienced an unprecedented outbreak of Eurasian clade 2.3.4.4 H5 highly pathogenic avian influenza (HPAI) virus, initially affecting mainly wild birds and few backyard and commercial poultry premises. To better model the outbreak, the pathogenesis and transmission dynamics of representative Eu...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2016.04.019
更新日期:2016-07-01 00:00:00
abstract::Enveloped virus entry occurs when viral and cellular membranes fuse releasing particle contents into the target cell. Human immunodeficiency virus (HIV) entry occurs by cell-free virus or virus transferred between infected and uninfected cells through structures called virological synapses. We developed a high-through...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2015.10.013
更新日期:2016-03-01 00:00:00
abstract::Torque Teno Virus (TTV) has been assigned to the floating genus Anellovirus. TTV ssDNA genomes have a size of 3.6 to 3.8 kb and display up to 30% nucleotide diversity. The pathogenic potential of TTV is under investigation. To address a putative link of pathogenicity with the observed sequence variations, the transcri...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.08.017
更新日期:2008-11-10 00:00:00
abstract::To study the possible role of immune selection in the in vivo generation of pathogenic recombinant murine leukemia viruses (MuLV), we have constructed recombinant vaccinia viruses (rVV) expressing the envelope genes of three MuLV: AKR623, MCF247, and MCF13. rVV expressing either AKR623 or MCF247 env could prime H-2b m...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1370
更新日期:1994-07-01 00:00:00
abstract::The open reading frame of the large subunit (R1) of herpes simplex virus type 1 (HSV-1) ribonucleotide reductase has been positioned downstream of the phage T7 gene 10 promoter in the expression vector, pET. Transformation of this recombinant plasmid into Escherichia coli BL21 DE3 cells containing the T7 RNA polymeras...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90627-n
更新日期:1991-06-01 00:00:00
abstract::A combination of linker scanning mutagenesis and deletional analyses has been used to determine the role of individual DNA-protein binding sites on expression from the hepatitis B virus (HBV) enhancer I-X promoter (map position (mp) 1042-1354, HBV adw2). Linker scanning mutation of the EF-C site caused a 67.5% drop in...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1173
更新日期:1993-04-01 00:00:00
abstract::EBNA-1 is the sole virally specified protein required for replication of the Epstein-Barr virus latency origin and also modulates its own expression. Both of these functions are mediated through specific DNA binding. We created a series of amino acid substitutions across the previously identified DNA-binding domain of...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1669
更新日期:1994-12-01 00:00:00
abstract::We investigated the localization of host mRNA during Rift Valley fever virus (RVFV) infection. Fluorescence in situ hybridization revealed that infection with RVFV altered the localization of host mRNA. mRNA accumulated in the nuclei of RVFV-infected but not mock-infected cells. Further, overexpression of the NSS gene...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2015.09.003
更新日期:2015-12-01 00:00:00
abstract::Coinfection of tobacco plants with potato virus X (PVX) and any of several members of the potyvirus group causes a synergistic disease characterized by a dramatic increase in symptom severity correlated with a 3- to 10-fold increase in the accumulation of PVX in the first systemically infected leaves. We have recently...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8488
更新日期:1997-04-28 00:00:00
abstract::Using the complete genome sequences of 19 coronavirus genomes, we analyzed the codon usage bias, dinucleotide relative abundance and cytosine deamination in coronavirus genomes. Of the eight codons that contain CpG, six were markedly suppressed. The mean NNU/NNC ratio of the six amino acids using either NNC or NNU as ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2007.08.010
更新日期:2007-12-20 00:00:00
abstract::Coronavirus small envelope protein E has two known biological functions: it plays a pivotal role in virus envelope formation, and the murine coronavirus E protein induces apoptosis in E protein-expressing cultured cells. The E protein is an integral membrane protein. Its C-terminal region extends cytoplasmically in th...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2001.0818
更新日期:2001-03-15 00:00:00
abstract::Human papillomaviruses (HPVs) infect epithelial cells and are associated with genital carcinoma. Most epithelial cell lines express cell-surface glycosaminoglycans (GAGs) usually found attached to the protein core of proteoglycans. Our aim was to study how GAGs influenced HPV entry. Using a human keratinocyte cell lin...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(03)00114-4
更新日期:2003-05-25 00:00:00
abstract::The adenovirus (Ad) single-stranded DNA binding protein (DBP) is a multifunctional protein. It is thought to consist of two domains, the amino-terminal domain involved in host-range determination and the carboxyl-terminal domain functioning in DNA replication and DNA binding. We have determined the nucleotide sequence...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90227-9
更新日期:1988-03-01 00:00:00
abstract::We have previously reported that a virus, MH2-PA200, lacking the ability to transform quail embryo cells, could be isolated from wild type (wt) MH2 stocks passaged on chicken neuroretina cells. We report here the molecular cloning and extensive characterization of this MH2-PA200 provirus. Molecularly cloned MH2-PA200 ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90030-9
更新日期:1986-09-01 00:00:00
abstract::The 130-kDa bovine herpesvirus-1 (BHV-1) glycoprotein GVP 6 was found to cross-react immunologically with the herpes simplex glycoprotein gB. Antibodies in polyclonal serum against gB immunoprecipitated GVP 6 and its cleavage products from a lysate of BHV-1-infected cells. Conversely, polyclonal serum against GVP 6 pr...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90525-9
更新日期:1988-10-01 00:00:00
abstract::The sequences of 189 residues from the 3' terminus of three RNAs of one strain and two RNAs of another strain of tomato aspermy virus have been determined; there was almost complete sequence homology between the RNAs. A base-paired transfer RNA-like structure is proposed for tomato aspermy virus RNAs which is similar ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(81)90639-5
更新日期:1981-07-15 00:00:00
abstract::The Autographa californica multiple nucleopolyhedrovirus (AcMNPV) occlusion-derived virus (ODV) envelope protein ODV-E56 is essential for oral infection of larvae of Heliothis virescens. Bioassays with recombinant clones of AcMNPV lacking a functional odv-e56 gene showed that ODV-E56 was required for infectivity of bo...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.09.027
更新日期:2011-01-05 00:00:00