Development and application of a blocking enzyme-linked immunosorbent assay (ELISA) to differentiate antibodies against live and inactivated porcine reproductive and respiratory syndrome virus.

abstract：:Cellular activation plays an important role in retroviral replication. Previously, we have shown that CD4(+)CD25(+) T cells by the virtue of their partially activated phenotype represent ideal candidates for a productive feline immunodeficiency virus (FIV) infection. In the present study, we extended our previous obse...

journal_title：Virology

authors： Joshi A,Garg H,Tompkins MB,Tompkins WA

更新日期：2005-05-10 00:00:00

abstract：:Hepatitis C virus (HCV) core protein is a multifunctional protein interacting with cellular and viral proteins and promoters. A tetracycline-regulated system was used to generate a HepG2 Tet-Off cell line allowing regulated expression of a full-length (191 aa) and an N(c)-truncated core protein (160 aa). In this syste...

journal_title：Virology

authors： Erhardt A,Hassan M,Heintges T,Häussinger D

更新日期：2002-01-20 00:00:00

abstract：:Enveloped virus entry occurs when viral and cellular membranes fuse releasing particle contents into the target cell. Human immunodeficiency virus (HIV) entry occurs by cell-free virus or virus transferred between infected and uninfected cells through structures called virological synapses. We developed a high-through...

journal_title：Virology

authors： Esposito AM,Cheung P,Swartz TH,Li H,Tsibane T,Durham ND,Basler CF,Felsenfeld DP,Chen BK

更新日期：2016-03-01 00:00:00

abstract：:The dependence of retroviral replication on cell proliferation was described as early as 1958, although different classes of retroviruses are able to infect non-dividing cells with different efficiencies. For example, the human immunodeficiency virus (HIV) and other lentiviruses infect most non-dividing cells nearly a...

journal_title：Virology

authors： Yamashita M,Emerman M

更新日期：2006-01-05 00:00:00

abstract：:Viral functions essential for the establishment of latent infection in murine sensory neurons in vivo were investigated by employing a herpes simplex virus type 1 (HSV-1) variant (KD6/B11) deleted for expression of the ICP4 gene and therefore unable to replicate. Since the viral DNA persisted in these cells, the laten...

journal_title：Virology

authors： Sedarati F,Margolis TP,Stevens JG

更新日期：1993-02-01 00:00:00

abstract：:The interferon (IFN) activity found in human leukocyte IFN alpha preparations, autoimmune and AIDS sera, and others was reported to have distinct antigenic and deviating biological properties. This led to its vague designation as acid-labile and thermolabile IFN alpha. However, using specific monoclonal antibodies, th...

journal_title：Virology

authors： Kontsek P,Borecký L,Novák M

更新日期：1991-03-01 00:00:00

abstract：:Within the family of Poxviridae, the entomopoxviruses are the most distant relatives of the more well-known and intensively studied orthopoxviruses (vaccinia and variola). The growth of the entomopoxvirus from Amsacta moorei (AmEPV) has been characterized in cell culture and compared to that of vaccinia virus (VV), th...

journal_title：Virology

authors： Winter J,Hall RL,Moyer RW

更新日期：1995-08-20 00:00:00

abstract：:The mouse mammary tumor virus (MMTV) is a type B retrovirus that is unique from other retroviruses in having multiple "tissue specific" and "hormone inducible" promoters. This unique feature has lead to the increasing interest in studying the biology of MMTV replication with the ultimate goal of developing MMTV based ...

journal_title：Virology

authors： Rizvi TA,Ali J,Phillip PS,Ghazawi A,Jayanth P,Mustafa F

更新日期：2009-03-15 00:00:00

abstract：:The influenza RNA polymerase is known to catalyse three distinct copying activities: (i) transcription of minus-sense virion RNA (vRNA) into mRNA, (ii) transcription of vRNA into full-length complementary RNA (cRNA), and (iii) transcription of cRNA to vRNA. Ever since the discovery of the conserved 13 and 12 long sequ...

journal_title：Virology

authors： Seong BL,Brownlee GG

更新日期：1992-01-01 00:00:00

abstract：:The genus Badnavirus is characterized by members that are genetically and serologically heterogeneous which presents challenges for their detection and characterization. The presence of integrated badnavirus-like sequences in some host species further complicates detection using PCR-based protocols. To address these c...

journal_title：Virology

authors： Sukal AC,Kidanemariam DB,Dale JL,Harding RM,James AP

更新日期：2019-03-01 00:00:00

abstract：:Infection of human helper T lymphocytes with the human immunodeficiency virus (HIV) results in a rapid induction of cytopathic effects and cell lysis. We isolated a variant of the human T-lymphoblastoid cell line, CEM, that is fully susceptible to HIV infection but resistant to virally induced cytopathic effects. Expo...

journal_title：Virology

authors： Casareale D,Stevenson M,Sakai K,Volsky DJ

更新日期：1987-01-01 00:00:00

abstract：:Proviruses were cloned directly from a cat that developed neurological disorders approximately 28 months after inoculation with a molecularly cloned, minimally pathogenic subgroup A feline leukemia virus (FeLV-A). In addition to FeLV-A proviruses that were nearly identical to the inoculated virus, we detected a subgro...

journal_title：Virology

authors： Boomer S,Gasper P,Whalen LR,Overbaugh J

更新日期：1994-11-01 00:00:00

abstract：:The polycation DEAE-dextran increased the binding of VSV to BHK cells about fourfold over a wide range of VSV concentrations. The same proportion of bound virions was internalized by the cell in the presence or absence of DEAE-dextran. Viral primary RNA production was increased to the equivalent of a 4- to 4.5-fold in...

journal_title：Virology

authors： Bailey CA,Miller DK,Lenard J

更新日期：1984-02-01 00:00:00

abstract：:The display of foreign proteins on the surface of baculovirus virions has provided a tool for the analysis of protein-protein interactions and for cell-specific targeting in gene transfer applications. To evaluate the baculovirus display system as a vaccine vehicle, we have generated a recombinant baculovirus (AcNPV-C...

journal_title：Virology

authors： Yoshida S,Kondoh D,Arai E,Matsuoka H,Seki C,Tanaka T,Okada M,Ishii A

更新日期：2003-11-10 00:00:00

abstract：:As porcine endogenous retroviruses (PERV) productively infect human cells in vitro, they pose a serious risk in xenotransplantation and xenogeneic cell therapies. We have analyzed the prevalence of six well-characterized full-length PERV, five of them being replication-competent and four of them being chromosomally as...

journal_title：Virology

authors： Niebert M,Tönjes RR

更新日期：2003-09-01 00:00:00

abstract：:High-risk human papillomaviruses (HPVs) are etiologically implicated in human cervical cancer. Two viral genes, E6 and E7, are commonly found expressed in these cancer cells. We have previously shown that mice transgenic for the HPV-16 E6 gene or E7 gene, in which the E6 or E7 was expressed in the basal layer of epith...

journal_title：Virology

authors： Song S,Liem A,Miller JA,Lambert PF

更新日期：2000-02-15 00:00:00

abstract：:Little is known concerning the function of the C-terminal nonconserved region of Sindbis virus nsP3. In this report, we created a number of in-frame deletions and duplications (from 12 to 159 residues) in this region and examined their effects on Sindbis virus replication. Sindbis RNA transcripts containing these muta...

journal_title：Virology

authors： Lastarza MW,Grakoui A,Rice CM

更新日期：1994-07-01 00:00:00

abstract：:Pandemic H1N1 influenza A (H1N1pdm) elicits stronger pulmonary inflammation than previously circulating seasonal H1N1 influenza A (sH1N1), yet mechanisms of inflammatory activation in respiratory epithelial cells during H1N1pdm infection are unclear. We investigated host responses to H1N1pdm/sH1N1 infection and virus ...

journal_title：Virology

authors： Paquette SG,Banner D,Chi le TB,Leόn AJ,Xu L,Ran L,Huang SS,Farooqui A,Kelvin DJ,Kelvin AA

更新日期：2014-01-05 00:00:00

abstract：:Therapeutic immunization of HIV-1-infected individuals may induce and/or enhance HIV-1-specific immune responses and decrease the dependency on antiretroviral drug treatment. However, repeated immunizations with live-recombinant vectors may induce vector-specific immune responses that interfere with the elicitation of...

journal_title：Virology

authors： Hel Z,Nacsa J,Tsai WP,Thornton A,Giuliani L,Tartaglia J,Franchini G

更新日期：2002-12-05 00:00:00

abstract：:The simian virus 40 (SV40) large T antigen was used as an immunogenic vector to express a herpes simplex virus type 1 (HSV-1) glycoprotein B (gB), H-2Kb-restricted cytotoxic T lymphocyte (CTL) recognition epitope corresponding to amino acid residues 498-505. Immunization of naive, C57BL/6 mice with a cell line, B6/350...

journal_title：Virology

authors： Bonneau RH,Fu TM,Tevethia SS

更新日期：1993-12-01 00:00:00

abstract：:In order to gain a better perspective on the true variability of varicella-zoster virus (VZV) and to catalogue the location and number of differences, 11 new complete genome sequences were compared with those previously in the public domain (18 complete genomes in total). Three of the newly sequenced genomes were deri...

journal_title：Virology

authors： Tyler SD,Peters GA,Grose C,Severini A,Gray MJ,Upton C,Tipples GA

更新日期：2007-03-15 00:00:00

abstract：:The interferon sensitivity of the expression of an influenza-virus hemagglutinin (HA) gene cloned into the thymidine kinase (TK) gene of vaccinia virus was studied in chick embryo fibroblasts (CEF) and Madin-Darby bovine kidney (MDBK) cells. In CEF, the expression of the HA gene is inhibited by pretreatment of cells w...

journal_title：Virology

authors： Degen HJ,Blum D,Grün J,Jungwirth C

更新日期：1992-05-01 00:00:00

abstract：:The alphaherpesviruses establish latent infections in sensory neuronal cells, during which a number of latency-associated RNA transcripts (LATs) are produced. The reasons for the production of the LATs, however, and the mechanism by which the LAT region of the genome remains active during latency are unknown. Here we ...

journal_title：Virology

authors： Coffin RS,Howard MK,Latchman DS

更新日期：1995-06-01 00:00:00

abstract：:Mycophenolic acid (MPA), an inhibitor of inosine monophosphate dehydrogenase, shows strong anti-HIV activity in vitro in both human peripheral blood CD4+ lymphocytes and macrophages, as well as established human cell lines. MPA shows its greatest antiviral effects during the early stages of HIV infection. By limiting ...

journal_title：Virology

authors： Ichimura H,Levy JA

更新日期：1995-08-20 00:00:00

abstract：:The DNA polymerase (DNApol) and major capsid protein (MCP) genes were used as models to study promoter activity in Chilo iridescent virus (CIV). Infection of Bombyx mori SPC-BM-36 cells in the presence of inhibitors of DNA or protein synthesis showed that DNApol, as well as helicase, is an immediate-early gene and con...

journal_title：Virology

authors： Nalçacioğlu R,Marks H,Vlak JM,Demirbaĝ Z,van Oers MM

更新日期：2003-12-20 00:00:00

abstract：:The human parvovirus B19 causes aplastic crises in sickle cell anemia patients and the disease erythema infectiosum. So far, it has not been possible to grow B19 virus in cultured cells. Here we report the use of in vitro transcription in HeLa cell extracts and transient expression of cloned DNA transfected into HeLa ...

journal_title：Virology

authors： Doerig C,Beard P,Hirt B

更新日期：1987-04-01 00:00:00

abstract：:Mouse mammary tumor virus (MMTV) is a pH-dependent virus that uses mouse transferrin receptor 1 (TfR1) for entry into cells. Previous studies demonstrated that MMTV could induce pH 5-dependent fusion-from-with of mouse cells. Here we show that the MMTV envelope-mediated cell-cell fusion requires both the entry recepto...

journal_title：Virology

authors： Wang E,Obeng-Adjei N,Ying Q,Meertens L,Dragic T,Davey RA,Ross SR

更新日期：2008-11-25 00:00:00

abstract：:Infection with a broad diversity of viruses often activates host cell signaling pathways including the mitogen-activated protein kinase pathway. The present study established that dengue virus infection of human macrophages activates Jun NH(2)-terminal kinase (JNK) and the p38 MAPKs pathways. The activation was observ...

journal_title：Virology

authors： Ceballos-Olvera I,Chávez-Salinas S,Medina F,Ludert JE,del Angel RM

更新日期：2010-01-05 00:00:00

abstract：:Tumor-inducing viruses like simian virus 40 or the human adenovirus produce oncoproteins which interfere with the cellular retinoblastoma (Rb) tumor-suppressor protein to create an appropriate molecular environment in the nucleus for viral transcription and replication. Such a strategy has been considered to be restri...

journal_title：Virology

authors： Collin S,Fernández-Lobato M,Gooding PS,Mullineaux PM,Fenoll C

更新日期：1996-05-01 00:00:00

abstract：:The Rice stripe virus (RSV) pc4 has been determined as the viral movement protein (MP). In this study, the pc4 gene was cloned into a movement-deficient Tobacco mosaic virus (TMV). The resulting hybrid TMV-pc4, in addition to spreading cell to cell in Nicotiana tabacum, moved systemically and induced foliar necrosis i...

journal_title：Virology

authors： Zhang C,Pei X,Wang Z,Jia S,Guo S,Zhang Y,Li W

更新日期：2012-04-10 00:00:00