Abstract:
:The display of foreign proteins on the surface of baculovirus virions has provided a tool for the analysis of protein-protein interactions and for cell-specific targeting in gene transfer applications. To evaluate the baculovirus display system as a vaccine vehicle, we have generated a recombinant baculovirus (AcNPV-CSPsurf) that displays rodent malaria Plasmodium berghei circumsporozoite protein (PbCSP) on the virion surface as a fusion protein with the major baculovirus envelope glycoprotein gp64. The PbCSP-gp64 fusion protein was incorporated and oligomerized on the virion surface and led to a 12-fold increase in the binding activity of AcNPV-CSPsurf virions to HepG2 cells. Immunization with adjuvant-free AcNPV-CSPsurf virions induced high levels of antibodies and gamma interferon-secreting cells against PbCSP and protected 60% of mice against sporozoite challenge. These data demonstrate that AcNPV-CSPsurf displays sporozoite-like PbCSP on the virion surface and possesses dual potentials as a malaria vaccine candidate and a liver-directed gene delivery vehicle.
journal_name
Virologyjournal_title
Virologyauthors
Yoshida S,Kondoh D,Arai E,Matsuoka H,Seki C,Tanaka T,Okada M,Ishii Adoi
10.1016/j.virol.2003.08.003subject
Has Abstractpub_date
2003-11-10 00:00:00pages
161-70issue
1eissn
0042-6822issn
1096-0341pii
S0042682203006007journal_volume
316pub_type
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