当前位置： SCI文献检索 > VIROLOGY期刊下所有文献 > Effect of C-terminal mutations of alfalfa mosaic virus coat protein on dimer formation and assembly in vitro.

Effect of C-terminal mutations of alfalfa mosaic virus coat protein on dimer formation and assembly in vitro.

Abstract：

:The coat protein (CP) of alfalfa mosaic virus (AMV) strain 425 assembles to bacilliform or rod-shaped particles in the presence of nucleic acids or to T = 1 empty icosahedral particles in the absence of nucleic acids. To study the determinants of CP assembly, recombinant CPs (rCPs) that contained a (His)(6) region were expressed in Escherichia coli. Wt rCP and a mutant rCP, which lacked the last nine amino acids of the C terminus (amino acids 213-221), assembled to particles that were identical in electron micrographs. However, a mutant rCP, which lacked the last 18 amino acids of the C terminus (amino acids 204-221), did not assemble. Likewise, a mutant with alanine substitutions at W(191), F(197), and P(198) did not assemble. Furthermore rCP with a single alanine substitution at W(191) did not assemble, whereas the rCP, which had an arginine and an alanine substitution at A(196) and F(197), respectively, formed rod-shaped particles. The mutations that prevented assembly prevented dimer formation, which indicates that dimers are the minimal building blocks of particles. Our results indicate that two separate regions in the C terminus of AMV CP are critical for dimer formation and assembly and that changes in key amino acids in one of the regions affect both assembly and particle morphology.

journal_name

Virology

journal_title

Virology

authors

Choi J,Loesch-Fries LS

doi

10.1006/viro.1999.9805

subject

Has Abstract

pub_date

1999-07-20 00:00:00

pages

182-9

issue

1

eissn

0042-6822

issn

1096-0341

pii

S0042-6822(99)99805-7

journal_volume

260

pub_type

杂志文章

相关文献

VIROLOGY文献大全
  • Fiber genes of adenoviruses with tropism for the eye and the genital tract.

    abstract：:We have characterized the fibergenes of adenovirus type 19p (Ad19p), Ad19a, and Ad37 by sequencing. The fiber genes of Ad19a and Ad37 are identical and only five amino acids differ comparing Ad19a/Ad37 with Ad19p. Based on the translated sequences we calculated the isoelectrical points (Ips) and found that the fiber k...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1996.8269

    authors： Arnberg N,Mei Yf,Wadell G

    更新日期：1997-01-06 00:00:00

  • Identification of a new neutralization epitope on VP7 of human serotype 2 rotavirus and evidence for electropherotype differences caused by single nucleotide substitutions.

    abstract：:Neutralizing monoclonal antibodies 2F1 and 1C10, which are specific for VP7 of serotype 2 rotaviruses (G2), were used to select neutralization escape variants of the human serotype 2 rotavirus, DS-1. Neutralization survival patterns generated by monoclonal antibodies 2F1, 1C10, and RV5:3 indicated that 2F1 and 1C10 di...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1993.1601

    authors： Dunn SJ,Ward RL,McNeal MM,Cross TL,Greenberg HB

    更新日期：1993-11-01 00:00:00

  • Endocytosis-mediated HIV-1 entry and its significance in the elusive behavior of the virus in astrocytes.

    abstract：:Astrocytes protect neurons but also evoke a proinflammatory response to injury and viral infections including HIV. We investigated the mechanism of HIV-1 infection in primary astrocytes, which showed minimal but productive viral infection independent of CXCR4. As with ectopic-CD4-expressing astrocytes, lysosomotropic ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2014.03.002

    authors： Chauhan A,Mehla R,Vijayakumar TS,Handy I

    更新日期：2014-05-01 00:00:00

  • HIV pre-exposure prophylaxis: mucosal tissue drug distribution of RT inhibitor Tenofovir and entry inhibitor Maraviroc in a humanized mouse model.

    abstract：:Pre-exposure prophylaxis (PrEP) strategies utilizing anti-retroviral drugs show considerable promise for HIV prevention. However there is insufficient pharmacokinetic (PK) data on drug concentrations required for protection at the relevant mucosal tissues where the infection is initiated. Here we evaluated the utility...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2014.07.008

    authors： Veselinovic M,Yang KH,LeCureux J,Sykes C,Remling-Mulder L,Kashuba ADM,Akkina R

    更新日期：2014-09-01 00:00:00

  • The minute virus of mice exploits different endocytic pathways for cellular uptake.

    abstract：:The minute virus of mice, prototype strain (MVMp), is a non-enveloped, single-stranded DNA virus of the family Parvoviridae. Unlike other parvoviruses, the mechanism of cellular uptake of MVMp has not been studied in detail. We analyzed MVMp endocytosis in mouse LA9 fibroblasts and a tumor cell line derived from epith...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2015.02.054

    authors： Garcin PO,Panté N

    更新日期：2015-08-01 00:00:00

  • Functional expression of influenza A viral nucleoprotein in cells transformed with cloned DNA.

    abstract：:Simian cells permissive for influenza A virus infection were stably transformed with a full-length cloned influenza A nucleoprotein gene under the control of an inducible metallothionein promoter and linked to a dihydrofolate reductase gene to facilitate cell selection. Transformed cells synthesized a virus-specific n...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(86)90437-x

    authors： Ryan KW,Mackow ER,Chanock RM,Lai CJ

    更新日期：1986-10-15 00:00:00

  • Porcine endogenous retroviruses inhibit human immune cell function: risk for xenotransplantation?

    abstract：:Transgenic pigs are currently the most favored potential source of organs for xenotransplantation. Like all mammalian species they all harbor endogenous retroviruses in their genome. These porcine endogenous retroviruses (PERVs) are produced from several primary cells and cell lines and are able to infect human cells....

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1999.0149

    authors： Tacke SJ,Kurth R,Denner J

    更新日期：2000-03-01 00:00:00

  • A reassortant between influenza A viruses (H7N2) synthesizing an enzymatically inactive neuraminidase at 40 degrees which is not incorporated into infectious particles.

    abstract：:Cells infected with a reassortant (113/Ho, H7N2) between A/fowl plague/Rostock/34 (FPV, H7N1) and A/Hong Kong/1/68 (H3N2) carrying RNA segments 1 and 6 of the Hong Kong virus and the residual genes of FPV, synthesized at 40 degrees a neuraminidase (NA) which is enzymatically not active and which is not incorporated in...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(86)90266-7

    authors： Breuning A,Scholtissek C

    更新日期：1986-04-15 00:00:00

  • A chimpanzee adenoviral vector-based rabies vaccine protects beagle dogs from lethal rabies virus challenge.

    abstract：:Rabies continues to poses serious threats to the public health in many countries. The development of novel inexpensive, safe and effective vaccines has become a high priority for rabies control worldwide. We previously generated a novel recombinant rabies vaccine by cloning rabies virus glycoprotein into a chimpanzee ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2019.07.022

    authors： Wang X,Fang Z,Xiong J,Yang K,Chi Y,Tang X,Ma L,Zhang R,Deng F,Lan K,Zhou D

    更新日期：2019-10-01 00:00:00

  • Characterization of the interactions among vaccinia virus transcription factors G2R, A18R, and H5R.

    abstract：:Prior genetic analysis suggests that there may exist an interaction between the products of the vaccinia virus genes A18R, a putative negative transcription elongation factor, and G2R, a putative positive transcription elongation factor. In addition, affinity purification of polyhistidine-tagged G2R protein overexpres...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1998.9166

    authors： Black EP,Moussatche N,Condit RC

    更新日期：1998-06-05 00:00:00

  • Analysis of proviruses integrated in Fli-1 and Evi-1 regions in Cas-Br-E MuLV-induced non-T-, non-B-cell leukemias.

    abstract：:The DNAs of the Cas-Br-E MuLV-induced leukemias always contain somatically acquired mink cell focus-forming (MCF) recombinant proviruses. MCF recombinants could be involved during leukemogenesis at both preleukemic times and in late-stage tumors. Among the Cas-Br-E-induced non-T-, non-B-cell leukemias, viral integrati...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(92)90241-g

    authors： Bergeron D,Poliquin L,Houde J,Barbeau B,Rassart E

    更新日期：1992-12-01 00:00:00

  • Turnip crinkle virus infection from RNA synthesized in vitro.

    abstract：:Genome-length cDNA clones of turnip crinkle virus (TCV) were constructed with SmaI and XbaI restriction sites engineered at the 5' and 3' termini, respectively. The genome-length cDNAs were positioned downstream of modified lambda and T7 phage promoters such that in vitro transcription resulted in RNAs with 5 extra nu...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(89)90368-1

    authors： Heaton LA,Carrington JC,Morris TJ

    更新日期：1989-05-01 00:00:00

  • Transforming properties of Felis catus papillomavirus type 2 E6 and E7 putative oncogenes in vitro and their transcriptional activity in feline squamous cell carcinoma in vivo.

    abstract：:Felis catus papillomavirus type 2 (FcaPV2) DNA is found in feline cutaneous squamous cell carcinomas (SCCs); however, its biological properties are still uncharacterized. In this study, we successfully expressed FcaPV2 E6 and E7 putative oncogenes in feline epithelial cells and demonstrated that FcaPV2 E6 binds to p53...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2016.05.017

    authors： Altamura G,Corteggio A,Pacini L,Conte A,Pierantoni GM,Tommasino M,Accardi R,Borzacchiello G

    更新日期：2016-09-01 00:00:00

  • Antigenic, sequence, and crystal variation in influenza B neuraminidase.

    abstract：:The neuraminidase (NA) genes of influenza B viruses B/Maryland/59, B/Hong Kong/8/73, B/Singapore/222/79, B/Oregon/5/80, B/USSR/100/83, B/Victoria/3/85, B/Leningrad/179/86, B/Memphis/6/86, and B/Memphis/3/89 have been sequenced. The deduced amino acid sequences show high variability in the stalk domain of the NA, but a...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(90)90523-t

    authors： Air GM,Laver WG,Luo M,Stray SJ,Legrone G,Webster RG

    更新日期：1990-08-01 00:00:00

  • Microevolution of tick-borne encephalitis virus in course of host alternation.

    abstract：:Two tick-borne encephalitis (TBE) virus variants were studied: mouse brain-adapted strain EK-328 and its derivate adapted to Hyalomma marginatum ticks. The tick-adapted virus exhibited small-plaque phenotype and slower replication in PEK cells, higher yield in ticks, decreased neuroinvasiveness in mice, increased bind...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2006.12.013

    authors： Romanova LIu,Gmyl AP,Dzhivanian TI,Bakhmutov DV,Lukashev AN,Gmyl LV,Rumyantsev AA,Burenkova LA,Lashkevich VA,Karganova GG

    更新日期：2007-05-25 00:00:00

  • Characterization of the immediate-early functions of pseudorabies virus.

    abstract：:The immediate-early transcripts of pseudorabies virus have been located in a region of the genome situated internally within the inverted repeat between map positions 0.99 and 0.95. A single immediate-early transcript (approximately 6 kb) can be detected both in the cytoplasmic and nuclear fractions of infected, cyclo...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(83)90510-x

    authors： Ihara S,Feldman L,Watanabe S,Ben-Porat T

    更新日期：1983-12-01 00:00:00

  • The Potyviridae P1a leader protease contributes to host range specificity.

    abstract：:The P1a protein of the ipomovirus Cucumber vein yellowing virus is one of the self-cleavage serine proteases present in Potyviridae family members. P1a is located at the N-terminal end of the viral polyprotein, and is closely related to potyviral P1 protease. For its proteolytic activity, P1a requires a still unknown ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2014.12.013

    authors： Shan H,Pasin F,Valli A,Castillo C,Rajulu C,Carbonell A,Simón-Mateo C,García JA,Rodamilans B

    更新日期：2015-02-01 00:00:00

  • Polymorphisms of the cytidine deaminase APOBEC3F have different HIV-1 restriction efficiencies.

    abstract：:The APOBEC3 enzyme family are host restriction factors that induce mutagenesis of HIV-1 proviral genomes through the deamination of cytosine to form uracil in nascent single-stranded (-)DNA. HIV-1 suppresses APOBEC3 activity through the HIV-1 protein Vif that induces APOBEC3 degradation. Here we compared two common po...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2018.11.004

    authors： Mohammadzadeh N,Follack TB,Love RP,Stewart K,Sanche S,Chelico L

    更新日期：2019-01-15 00:00:00

  • Identification of viral membrane proteins required for cell fusion and viral dissemination that are modified during vaccinia virus persistence.

    abstract：:Wild-type vaccinia virus WR strain forms non-fusogenic (F-) large plaques and is hemagglutinin positive (HA+) under normal conditions of virus infection. We have analyzed a collection of spontaneous, highly attenuated mutants of vaccinia virus isolated from persistently infected Friend erythroleukemia cells (E. Paez, ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1994.1018

    authors： Ortiz MA,Paez E

    更新日期：1994-01-01 00:00:00

  • Induction of secreted human immunodeficiency virus type 1 (HIV-1) resistance factors in CD4-positive T lymphocytes by attenuated HIV-1 infection.

    abstract：:This report describes induction of HIV-1 resistance and synthesis of resistance factors in immortal CD4-positive T lymphocytes. SupT1 cells were infected by NL4-3 attenuated by a defect in the vif gene through coculture with infected primary lymphocytes. Cell lines from this infection, termed R1, expressed CD4 and CXC...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.2001.1300

    authors： Simm M,Miller LS,Durkin HG,Allen M,Chao W,Lesner A,Potash MJ,Volsky DJ

    更新日期：2002-03-01 00:00:00

  • Molecular cloning and characterization of MSRV-related sequences associated with retrovirus-like particles.

    abstract：:New sequences have been obtained by successive overlapping RT-PCR extensions from the pol region of a retroviral RNA (multiple sclerosis-associated retroviral element, MSRV) amplified in retrovirus-like particles from patients with multiple sclerosis. gag and pol sequences are related to type C oncoviruses, whereas th...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1999.9792

    authors： Komurian-Pradel F,Paranhos-Baccala G,Bedin F,Ounanian-Paraz A,Sodoyer M,Ott C,Rajoharison A,Garcia E,Mallet F,Mandrand B,Perron H

    更新日期：1999-07-20 00:00:00

  • Membranes of herpes simplex virus type-1-infected human corneal epithelial cells are not permeabilized to macromolecules and therefore do not release IL-1alpha.

    abstract：:Nanogram amounts of the proinflammatory cytokine interleukin-1alpha (IL-1alpha) were detected in uninfected cultures of human corneal epithelial cells (HCEC). Although HSV-1 replicated >10(4)-fold in these cells and caused extensive cytopathic effects, virus infection was not accompanied by significant extracellular r...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1998.9084

    authors： Tran MT,Dean DA,Lausch RN,Oakes JE

    更新日期：1998-04-25 00:00:00

  • GM-CSF DNA: an adjuvant for higher avidity IgG, rectal IgA, and increased protection against the acute phase of a SHIV-89.6P challenge by a DNA/MVA immunodeficiency virus vaccine.

    abstract：:Single intradermal or intramuscular inoculations of GM-CSF DNA with the DNA prime for a simian-human immunodeficiency virus (SHIV)-89.6 vaccine, which consists of DNA priming followed by modified vaccinia Ankara (MVA) boosting, increased protection of both the blood and intestines against the acute phase of an intrare...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2007.07.017

    authors： Lai L,Vödrös D,Kozlowski PA,Montefiori DC,Wilson RL,Akerstrom VL,Chennareddi L,Yu T,Kannanganat S,Ofielu L,Villinger F,Wyatt LS,Moss B,Amara RR,Robinson HL

    更新日期：2007-12-05 00:00:00

  • A nucleotide sequence rearrangement distinguishes two isolates of satellite tobacco ringspot virus RNA.

    abstract：:Several strains of tobacco ringspot virus (TobRV) support the replication and encapsidation of satellite tobacco ringspot virus RNA (STobRV RNA). We have compared the nucleotide sequences of four STobRV RNAs, each initially associated with a different isolate of TobRV. A STobRV RNA from a geranium isolate of TobRV and...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(87)90049-3

    authors： Buzayan JM,McNinch JS,Schneider IR,Bruening G

    更新日期：1987-09-01 00:00:00

  • Latent murine cytomegalovirus infection in macrophages.

    abstract：:In this study we show that macrophages (Mphi) are latently infected with murine cytomegalovirus (MCMV). After clearance of acute MCMV infection, the predominant form of chronic infection in Balb mice is latency rather than persistence. Peritoneal exudate cells (PECs) from latently infected Balb mice (3-9 months postin...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1996.8303

    authors： Pollock JL,Presti RM,Paetzold S,Virgin HW 4th

    更新日期：1997-01-06 00:00:00

  • Autographa californica multiple nucleopolyhedrovirus ac142, a core gene that is essential for BV production and ODV envelopment.

    abstract：:Autographa californica multiple nucleopolyhedrovirus (AcMNPV) ac142 is a baculovirus core gene and encodes a protein previously shown to associate with occlusion-derived virus (ODV). To determine its role in the baculovirus life cycle, we used the AcMNPV bacmid system to generate an ac142 deletion virus (AcBAC(ac142KO...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2007.10.019

    authors： McCarthy CB,Dai X,Donly C,Theilmann DA

    更新日期：2008-03-15 00:00:00

  • H4N8 subtype avian influenza virus isolated from shorebirds contains a unique PB1 gene and causes severe respiratory disease in mice.

    abstract：:H4N8 subtype avian influenza viruses were isolated from shorebirds in eastern Hokkaido. All the isolates shared >99.7% nucleotide homology, and all the viral genes except for PB1 were highly related to those of A/red-necked stint/Australia/1/04. Thus, the isolates were regarded as PB1 reassortants. The most similar PB...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2011.11.019

    authors： Bui VN,Ogawa H,Xininigen,Karibe K,Matsuo K,Awad SS,Minoungou GL,Yoden S,Haneda H,Ngo LH,Tamaki S,Yamamoto Y,Nakamura K,Saito K,Watanabe Y,Runstadler J,Huettmann F,Happ GM,Imai K

    更新日期：2012-02-05 00:00:00

  • Primary human cervical carcinoma cells require human papillomavirus E6 and E7 expression for ongoing proliferation.

    abstract：:Repression of human papillomavirus (HPV) E6 and E7 oncogenes in established cervical carcinoma cell lines causes senescence due to reactivation of cellular tumor suppressor pathways. Here, we determined whether ongoing expression of HPV16 or HPV18 oncogenes is required for the proliferation of primary human cervical c...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2011.10.012

    authors： Magaldi TG,Almstead LL,Bellone S,Prevatt EG,Santin AD,DiMaio D

    更新日期：2012-01-05 00:00:00

  • Inhibition of vaccinia virus replication by N-(phosphonoacetyl)-L-aspartate: differential effects on viral gene expression result from a reduced pyrimidine nucleotide pool.

    abstract：:The replication of vaccinia virus was reduced by 3 logs in cells that had been treated before and during infection with a concentration of N-(phosphonoacetyl)-L-aspartate (PALA) which lowered the UTP and CTP to 5 and 20% of controls, respectively, without affecting cell viability. The antiviral activity of PALA was re...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1997.8735

    authors： Katsafanas GC,Grem JL,Blough HA,Moss B

    更新日期：1997-09-15 00:00:00

  • Geographic classification of dengue-2 virus strains by antigen signature analysis.

    abstract：:Dengue-2 virus strains from different locations were compared by T1-RNAse-resistant oligonucleotide fingerprinting and antigen signature analysis. The latter technique involved construction of radioimmunoassays using monoclonal antibodies that recognize nine distinct dengue-2 type-specific and flavivirus cross-reactiv...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(86)90457-5

    authors： Monath TP,Wands JR,Hill LJ,Brown NV,Marciniak RA,Wong MA,Gentry MK,Burke DS,Grant JA,Trent DW

    更新日期：1986-10-30 00:00:00