Abstract:
:Genome-length cDNA clones of turnip crinkle virus (TCV) were constructed with SmaI and XbaI restriction sites engineered at the 5' and 3' termini, respectively. The genome-length cDNAs were positioned downstream of modified lambda and T7 phage promoters such that in vitro transcription resulted in RNAs with 5 extra nucleotides at the 3' end, and 1, 2, or 14 extra nucleotides at the 5' end depending on the construction. Transcripts with 14 extraviral 5' nucleotides were not infectious, while transcripts with 1 or 2 additional 5' nucleotides, with or without 5'-cap analog included in transcription reactions, were biologically active. These were approximately an order of magnitude less infectious than RNA extracted from TCV virions. The additional 5' nucleotides were not maintained in progeny viral RNAs isolated from plants.
journal_name
Virologyjournal_title
Virologyauthors
Heaton LA,Carrington JC,Morris TJdoi
10.1016/0042-6822(89)90368-1subject
Has Abstractpub_date
1989-05-01 00:00:00pages
214-8issue
1eissn
0042-6822issn
1096-0341journal_volume
170pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::Without a virus culture system, genetic analysis becomes the principal method to classify norovirus (NoV) strains. Currently, classification of NoV strains beneath the species level has been based on sequences from different regions of the viral genome. As a result, the phylogenetic insights of some virus were not app...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.11.015
更新日期:2006-03-15 00:00:00
abstract::The adenine DNA methyltransferase M.CviRI (TGCmA) gene from chlorella virus XZ-6E was cloned into the Autographa californica nuclear polyhedrosis virus (AcMNPV) genome and expressed in Spodoptera frugiperda insect cells under the control of two tandemly arranged viral promoters, the early ETL promoter and the late pol...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1539
更新日期:1993-10-01 00:00:00
abstract::We have shown that polyamide nucleic acids (PNAs) targeted to the PBS (PNA(PBS)) and A-loop (PNA(A-loop)) sequences, when transfected into cells, inhibit HIV-1 replication by blocking the initiation of reverse transcription via destabilizing tRNA(3)(Lys) primer from the viral genome. Here we demonstrate that both PNA(...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2007.01.016
更新日期:2007-06-20 00:00:00
abstract::The HIV-2 viral accessory protein Vpx is related to, but distinct from the Vpr protein of HIV-1. Vpx is packaged into virions and as a component of the viral preintegration complex (PIC) is required for efficient virus replication in non-dividing cells. We have previously reported that the minimal transferable region ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.10.036
更新日期:2006-03-01 00:00:00
abstract::To better understand the role of specific residues within the duck hepatitis B virus (DHBV) pre-S protein in neutralization and infectivity, we have selected and identified pre-S variants which escape neutralization. A highly neutralizing monoclonal antibody (Mab 900) which recognizes an epitope 83IPQPQWTP90 localized...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8665
更新日期:1997-08-04 00:00:00
abstract::Nipah virus (NV) and Hendra virus (HV) are recently emergent, related viruses that can cause severe disease in humans and animals. The goal of this study was to investigate the immunogenic and functional properties of the fusion (F) and attachment (G) glycoproteins of NV. Vaccination of mice with recombinant vaccinia ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2002.1418
更新日期:2002-04-25 00:00:00
abstract::The genes of the RF strain of bovine rotavirus have been cloned into pBR 322 following the synthesis and hybridization of cDNA transcribed from both strands of in vitro polyadenylated genomic RNA. Cloned rotavirus DNAs were assigned to most of the 11 genomic RNA segments by Northern blot hybridization. The complete se...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90159-4
更新日期:1984-10-15 00:00:00
abstract::Recently, we reported that PrP(Sc), a surrogate marker for prion disease, is associated with the cellular fraction of blood from scrapie-infected sheep using a ligand-based immunoassay. In the study reported here, we found that a subset of peripheral blood mononuclear cells is most likely to sequester PrP(Sc) during b...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.05.023
更新日期:2010-09-15 00:00:00
abstract::Studies based on low-stringency hybridizations of radiolabeled swinepox virus (SPV) DNA to Southern blots containing DNA of representative members of the Orthopoxvirus, Leporipoxvirus, and Avipoxvirus genera and the Entomopoxvirus subfamily have revealed no DNA homology at this level of resolution. Antigenic relatedne...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90040-i
更新日期:1991-01-01 00:00:00
abstract::In genetically susceptible C57BL/6 mice the LP-BM5 isolate of murine retroviruses causes profound splenomegaly, lymphadenopathy, hypergammaglobulinemia, and an immunodeficiency syndrome bearing many similarities to the pathologies seen in AIDS. Because of these similarities, which also include terminal B cell lymphoma...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8970
更新日期:1998-02-15 00:00:00
abstract::Antigenic sites on the G1 glycoprotein of La Crosse bunyavirus were defined by constructing a panel of neutralizing and nonneutralizing monoclonal antibodies (F. Gonzalez-Scarano, R. E. Shope, C. H. Calisher, and N. Nathanson (1982), Virology 120, 42-53). To analyze the relationship between the individual epitopes del...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90283-1
更新日期:1985-07-30 00:00:00
abstract::Lipid binding properties which are dependent on exposure to acid pH are an important mechanism for the cellular entry pathway for some enveloped viruses and for other macromolecules. Cell-to-cell fusion can be used to demonstrate this function. La Crosse virus, a member of the family Bunyaviridae, fused BHK-21 cells f...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90107-7
更新日期:1984-01-15 00:00:00
abstract::The HIV-1 accessory gene product Vpr can inhibit cell proliferation via cell cycle arrest at the G(2) phase, and it can induce apoptosis after G(2) arrest. We found recently that C81, a carboxy-terminally truncated form of Vpr, induced apoptosis via G(1) arrest but did not induce G(2) arrest of the cell cycle. Thus, i...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2000.0534
更新日期:2000-10-10 00:00:00
abstract::Friend murine leukemia virus (Fr-MLV) produces unspliced mRNAs, as well as singly-spliced mRNAs by which the Env protein is generated. We investigated the role of the intron within the Fr-MLV gene in Env expression using vectors with serially truncated introns. The up-regulatory regions, the 361-878 and the 5135-5399 ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.11.040
更新日期:2009-03-01 00:00:00
abstract::From two human brain tumors SV40 genomes were isolated by recombinant DNA techniques. The SV40 genome cloned from a human meningioma DNA was shown to be indistinguishable from wild-type SV40. In contrast, the SV40 genome cloned from a human astrocytoma proved to be a nonviable deletion mutant with a truncated early re...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90357-x
更新日期:1984-10-30 00:00:00
abstract::The induction of autoimmunity by viruses has been hypothesized to occur by a number of mechanisms. Coxsackievirus B4 (CB4) induces hyperglycemia in SJL mice resembling diabetes in humans. While virus is effectively cleared within 2 weeks, hyperglycemia does not appear until about 8-12 weeks postinfection at a time whe...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(03)00462-8
更新日期:2003-09-30 00:00:00
abstract::A natural variation is observed at position 4 of the 3'-end of influenza A virus genomes, where U (U4) or C (C4) is present. The replicon activity of C4 was 28% of U4. We compared the transcription and replication activity of U4 [v84(U4)], C4 [v84(C4)] and the complimentary RNA (c84) using the purified influenza virus...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.09.022
更新日期:2010-12-20 00:00:00
abstract::In the absence of an effective vaccine against HIV, it is urgent to develop an effective alternative such as a microbicide. Single and repeated applications of MC1220 microbicide were evaluated in macaques. First, animals were given a single application of 0.5% or 1.5% MC1220-containing liposomal gel. A second group w...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.06.008
更新日期:2010-09-15 00:00:00
abstract::We have characterized capsids made by seven temperature-sensitive (ts) mutants of HSV-1 previously shown to be defective in viral DNA processing and packaging at the nonpermissive temperature (NPT). The empty capsids isolated from mutant-infected cells at the NPT were devoid of DNA, cosedimented in sucrose with wt B c...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90288-7
更新日期:1988-04-01 00:00:00
abstract::Human cytomegalovirus (HCMV) glycoprotein B (gB) promotes virion entry into cells by fusing the virion envelope with the cellular membrane. We recently reported that UB cells (U373 glioblastoma cells constructed to produce HCMV gB constitutively) form multinucleate syncytia that are dependent on the density of gB in t...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.1290
更新日期:1995-06-01 00:00:00
abstract::Mammalian type C retroviruses can be detected by their induction of foci of cell transformation in S+I - cells. We have noted that certain subtypes of the mouse xenotropic type C retrovirus (MuLV) inhibit this cell alteration. This inhibition, associated with intact virions, is irreversible and gives the infected cell...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(87)90044-4
更新日期:1987-09-01 00:00:00
abstract::Adenovirus vectors are known to induce certain genes and impact innate response networks, but a broad understanding of this process and its mechanisms is currently lacking. For this reason, we chose to investigate and characterize Ad innate immunity using homogeneous, primary MEF cells replete with all the elements of...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.08.041
更新日期:2007-02-20 00:00:00
abstract::Herpes simplex virus 1 (HSV-1) infection induces changes to the host cell nucleus including relocalization of the cellular protein Upstream Binding Factor (UBF) from the nucleolus to viral replication compartments (VRCs). Herein, we tested the hypothesis that UBF is recruited to VRCs to promote viral DNA replication. ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2015.04.003
更新日期:2015-09-01 00:00:00
abstract::Avarol is a sesquiterpenoid hydroquinone, which displays no inhibitory potencies on mammalian DNA polymerases alpha, beta, and gamma, on mammalian RNA polymerases I, II, and III, or on reverse transcriptases from Moloney murine leukemia virus (Mo-MuLV) and from HIV. For a further elucidation of the antiviral effect of...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90596-x
更新日期:1988-08-01 00:00:00
abstract::The differential symptom determinants of the Holmes' masked (M) and U1 strains of tobacco mosaic virus previously were mapped to the 5'-coterminal open reading frame (ORF) encoding the 126-kDa protein and the N-terminal two-thirds of the 183-kDa protein. Both proteins influence viral RNA accumulation, but the function...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0368
更新日期:1996-07-01 00:00:00
abstract::In order to investigate the genome organization of the porcine epidemic diarrhea virus (PEDV) further, cDNA clones covering the region between the nucleocapsid and the spike (S) protein genes were independently constructed and sequenced for the two virulent isolates Br1/87 and CV777. Of the three major ORFs identified...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1058
更新日期:1994-02-01 00:00:00
abstract::Kaposi's sarcoma (KS)-associated herpesvirus (KSHV) vFLIP, a latent gene of KSHV, was first identified as a FLICE-inhibitory protein (FLIP) protecting cells from apoptosis. The vFLIP protein has been shown to activate the NF-κB signaling involved in spindle morphology formation both in HUVECs infected with KSHV and Ka...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2018.07.007
更新日期:2018-09-01 00:00:00
abstract::Whole genome sequences for three Epstein-Barr virus strains (B95-8, GD1 and AG876) were aligned and compared. In addition to known variable loci (including type-specific alleles for the EBNA2, EBNA3A, EBNA3B and EBNA3C genes, plus the EBNA1 and LMP1 genes), seven large-scale regions of lower-level diversity were ident...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.10.009
更新日期:2007-03-01 00:00:00
abstract::Few human endogenous retroviruses (HERVs) have been extensively studied in non-human primates. Such investigations have demonstrated that several element classes are primate unique, contain members with important biological function, are conserved in specific primate lineages, and have in some cases expanded in copy n...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.01.045
更新日期:2005-04-10 00:00:00
abstract::There are more than 100 known adenovirus serotypes, including 50 human serotypes. They can infect all 5 major vertebrate classes but only Aviadenovirus infecting birds and Mastadenovirus infecting mammals have been well studied. CELO (chicken embryo lethal orphan) adenovirus is responsible for mild respiratory patholo...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.05.011
更新日期:2008-08-15 00:00:00