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Influenza virus genome C4 promoter/origin attenuates its transcription and replication activity by the low polymerase recognition activity.

Abstract：

:A natural variation is observed at position 4 of the 3'-end of influenza A virus genomes, where U (U4) or C (C4) is present. The replicon activity of C4 was 28% of U4. We compared the transcription and replication activity of U4 [v84(U4)], C4 [v84(C4)] and the complimentary RNA (c84) using the purified influenza virus RNA polymerase in vitro. ApG-primed replication activities of v84(C4) and c84 were 23.8% and 7.8% of v84(U4). Globin mRNA-primed transcription activities of v84(C4) and c84 were 36.9% and 6.81% of v84(U4). De novo replication activities of v84(C4) and c84 were 21.3 and 10.2% of v84(U4). This difference came from their polymerase binding activity. When all the eight genome segments of WSN strain were changed to U4, the virus titer was 760 times higher than the wild type. However, its pathogenicity in mice was lower than the wild type.

journal_name

Virology

journal_title

Virology

authors

Jiang H,Zhang S,Wang Q,Wang J,Geng L,Toyoda T

doi

10.1016/j.virol.2010.09.022

subject

Has Abstract

pub_date

2010-12-20 00:00:00

pages

190-6

issue

2

eissn

0042-6822

issn

1096-0341

pii

S0042-6822(10)00610-0

journal_volume

408

pub_type

杂志文章

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