Abstract:
:Proviruses were cloned directly from a cat that developed neurological disorders approximately 28 months after inoculation with a molecularly cloned, minimally pathogenic subgroup A feline leukemia virus (FeLV-A). In addition to FeLV-A proviruses that were nearly identical to the inoculated virus, we detected a subgroup B-like variant in brain, bone marrow, and lymph node that apparently had acquired the major portion of its extracellular envelope gene (gp70) from endogenous FeLV-related sequences. A similar recombinant was also detected, by PCR, at low levels in bone marrow from an early time postinfection (2.5 months). A full-length proviral variant with this recombinant structure cloned from brain tissue encoded a replication-defective virus. A chimera encoding the 5' gag-pol portion of FeLV-A and the 3' env-LTR portion of the defective brain-derived clone was replication-competent and had the extended host range properties of FeLV-B.
journal_name
Virologyjournal_title
Virologyauthors
Boomer S,Gasper P,Whalen LR,Overbaugh Jdoi
10.1006/viro.1994.1597subject
Has Abstractpub_date
1994-11-01 00:00:00pages
805-10issue
2eissn
0042-6822issn
1096-0341pii
S0042-6822(84)71597-2journal_volume
204pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::RNA-binding proteins of coronavirus MHV-A59 were identified using an RNA overlay-protein blot assay (ROPBA). The major viral RNA-binding protein in virions and infected cells was the phosphorylated nucleocapsid protein N (50K). A new 140K virus structural protein was identified as a minor RNA-binding protein both in v...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90305-3
更新日期:1986-04-30 00:00:00
abstract::Previous in vitro work demonstrated the incorporation of the major inducible 70k heat shock protein (i.e., 72k HSP) into the biologically active light nucleocapsid (L-NC) variant of canine distemper virus (CDV). Here, in vitro induction of the cellular stress response, characterized by elevated cytoplasmic and intranu...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1072
更新日期:1993-02-01 00:00:00
abstract::Like their animal-infecting counterparts, plant bunyaviruses use capped RNA leaders cleaved from host cellular mRNAs to prime viral genome transcription in a process called cap-snatching, but in vivo systems to investigate the details of this process are lacking for them. Here, we report that Rice stripe tenuivirus (R...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2019.10.017
更新日期:2020-01-02 00:00:00
abstract::Rotaviruses are the leading cause of severe diarrhea in infants and young children worldwide. Thus, the development of an effective rotavirus vaccine is a major public health goal. This study was performed to identify the gene or genes responsible for rotavirus virulence or host range restriction and attenuation in a ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.1255
更新日期:1995-05-10 00:00:00
abstract::Human papillomaviruses (HPVs) are epitheliotropic viruses, and their life cycle is intimately linked to the stratification and differentiation state of the host epithelial tissues. Defining a role for the E5 gene product in the differentiation-dependent viral life cycle has been difficult due to the lack of a suitable...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9262
更新日期:1998-09-01 00:00:00
abstract::Putative replication complexes of cauliflower mosaic virus (CaMV) which resemble hepatitis B virus replication complexes in several respects have been identified. The CaMV replication complexes contain a heterogeneous population of minus-strand DNA ranging in size from less than 600 nucleotides to full genome length o...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90109-6
更新日期:1985-05-01 00:00:00
abstract::Our study reports the occurrence of HIV-1 insertional activation events in vivo. Using a previously described PCR assay, small aliquots of broncho-alveolar lavage (BAL) cells obtained from AIDS patients were analyzed. Nine percent (5/54) of the aliquots contained proviral-host sequence transcripts indicating HIV-1 pro...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.1161
更新日期:1995-04-01 00:00:00
abstract::Infection of adherent cell monolayers using a liquid inoculum represents an established method to reliably and quantitatively study virus infection, but poorly recapitulates the exposure and infection of cells in the respiratory tract that occurs during infection with aerosolized pathogens. To better simulate natural ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2016.10.007
更新日期:2017-01-01 00:00:00
abstract::Sulfation is a common post-translational modification of secreted and membrane proteins, with the sulfate attached to tyrosine residues or to glycan side-chains. I have shown that varicella-zoster virus (VZV) envelope glycoproteins gpI, gpII, and gpIII can be labeled with [35S]sulfate. The predominant VZV glycoprotein...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1576
更新日期:1993-11-01 00:00:00
abstract::It is now well recognized that mutations in the hepatitis B virus (HBV) genome occur during the natural course of chronic viral infection. Regions of the viral genome that are frequently affected by such mutations, rearrangements, and/or deletions generally involve the precore promoter, precore, and core as well as th...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8594
更新日期:1997-07-07 00:00:00
abstract::Whole genome sequences for three Epstein-Barr virus strains (B95-8, GD1 and AG876) were aligned and compared. In addition to known variable loci (including type-specific alleles for the EBNA2, EBNA3A, EBNA3B and EBNA3C genes, plus the EBNA1 and LMP1 genes), seven large-scale regions of lower-level diversity were ident...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.10.009
更新日期:2007-03-01 00:00:00
abstract::Hantaan virus, the prototype virus of hemorrhagic fever with renal syndrome, was examined for nucleic acid characteristics which would support its previously proposed inclusion in the virus family Bunyaviridae. Nucleocapsid RNA from Hantaan virions and a control bunyavirus were examined for ribonuclease A (RNase A) se...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(83)90514-7
更新日期:1983-12-01 00:00:00
abstract::Immunoelectron microscopy using an anti-VP2 monoclonal antibody complexed to colloidal gold has been used to study the mechanism of bluetongue virus (BTV) release from infected cells. Examination of the BTV-infected cell surface revealed that viruses are released both as enveloped particles, by budding through the pla...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90218-3
更新日期:1989-11-01 00:00:00
abstract::Equine infectious anemia virus (EIAV) is responsible of acute disease episodes characterized by fever, anemia, thrombocytopenia and anorexia in equids. The high mutation rate in EIAV genome limited the number of full genome sequences availability. In the present study, we used the SureSelect target enrichment system w...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2019.08.016
更新日期:2019-11-01 00:00:00
abstract::Ten mutations were introduced into the JC virus (JCV) T antigen within a region corresponding to the SV40 T-antigen DNA binding domain (SV40 amino acids 131 to 220); nine of these increased homology between the two proteins in sequences critical for SV40 T antigen DNA binding. All mutant JCV T antigens bound to JCV an...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90136-y
更新日期:1991-07-01 00:00:00
abstract::Tomato golden mosaic virus (TGMV) is a bipartite member of the subgroup III Geminiviridae. Like all geminiviruses, TGMV replicates in the nucleus of susceptible cells by rolling circle replication (RCR). Double-stranded replicative form DNA generated during RCR serves as template for the transcription of viral genes b...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8549
更新日期:1997-06-09 00:00:00
abstract::Infection of Salmonella enterica with lytic mutants of either P22 or SE1 bacteriophages triggers the expression of its DNA damage-inducible SOS response through a lexA-dependent pathway. This induction of the SOS system strictly requires the presence of the bacteriophage kil gene. Accordingly, plasmid overexpression o...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.04.001
更新日期:2006-08-01 00:00:00
abstract::The biological phenotype of HIV-1 isolates from 21 subjects with primary HIV-1 infection was determined in primary lymphocytes and monocyte-derived macrophages as well as in three T-cell lines (MT-2, HUT78, and C8166). Seven isolates (33%) replicated on at least one cell line and were classified as rapid/high (n = 6) ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1534
更新日期:1994-10-01 00:00:00
abstract::Analyses of reassortant and parental strains of BTV serotypes 3 and 10, in serum neutralization tests, confirmed the major role of outer capsid protein VP2 in determination of virus serotype and its involvement in serum neutralization. However, a reassortant BTV strain (R70), containing protein VP5 derived from BTV 3 ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90447-9
更新日期:1989-06-01 00:00:00
abstract::Currently, little is known about the ankyrin/F-box protein B4. Here, we report that B4R-null viruses exhibited reduced plaque size in tissue culture, and decreased ability to spread, as assessed by multiple-step growth analysis. Electron microscopy indicated that B4R-null viruses still formed mature and extracellular ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2014.03.019
更新日期:2014-05-01 00:00:00
abstract::Expression of adenosine deaminase acting on RNA1 (ADAR1) is driven by alternative promoters. Promoter PA, activated by interferon (IFN), produces transcripts that encode the inducible p150 ADAR1 protein, whereas PB specifies the constitutively expressed p110 protein. We show using Stat1(-/-), Stat2(-/-) and IRF9(-/-) ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2015.08.001
更新日期:2015-11-01 00:00:00
abstract::Human immunodeficiency virus type 1 (HIV-1) infection can be inhibited by small molecules that target the CCR5 coreceptor. Here, we describe some properties of clonal viruses resistant to one such inhibitor, SCH-D, using both chimeric, infectious molecular clones and Env-pseudotypes. Studies using combinations of CCR5...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.11.004
更新日期:2007-04-25 00:00:00
abstract::The parapoxvirus Orf virus (ORFV) encodes several immunomodulatory proteins (IMPs) that modulate host-innate and pro-inflammatory responses and has been proposed as a vaccine delivery vector for use in animal species. Here we describe the construction and characterization of two recombinant ORFV vectors expressing the...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2017.08.027
更新日期:2017-11-01 00:00:00
abstract::Two revertants of ts110 Moloney murine sarcoma virus (MuSV) with wild-type MuSV phenotype were examined for the presence of mos gene products, ts110 MuSV has a temperature-sensitive defect in a function required to maintain the transformed phenotype. The nonproducer 6m2 cell clone transformed by ts110 produces an 85,0...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(83)90483-x
更新日期:1983-04-15 00:00:00
abstract::Section of the sciatic nerve during the period of herpes simplex virus (HSV) latent infection was performed to evaluate residual latency in mouse dorsal root ganglion. In control mice without sciatic neurectomy, latency was present in 90-100%, while in those which underwent a neurectomy procedure, latent infection was...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90085-2
更新日期:1988-11-01 00:00:00
abstract::The influenza RNA polymerase is known to be important in pathogenicity and adaptation of avian influenza viruses to mammalian hosts. However, the molecular mechanisms responsible are only partly understood. Here we investigated the role of the polymerase in two different, closely related, H5N1 influenza viruses - a hi...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.01.036
更新日期:2010-05-25 00:00:00
abstract::The avian hematopoietic system has long been an invaluable model to study the mechanisms of cell growth and differentiation. We have developed six MAbs against either chicken embryonic hematopoietic precursor cells or retrovirus-transformed cells. MAbs Mo1, Mo2, and Mo3 recognized transformation-associated markers exp...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90581-9
更新日期:1992-08-01 00:00:00
abstract::Encephalomyocarditis virus (EMCV) is the prototype member of the cardiovirus genus of picornaviruses. For cardioviruses and the related aphthoviruses, the first protein segment translated from the plus-strand RNA genome is the Leader protein. The aphthovirus Leader (173-201 amino acids) is an autocatalytic papain-like...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2001.1193
更新日期:2001-11-25 00:00:00
abstract::Recently, we identified TT virus (TTV) isolates from nonhuman primates and named them simian TTV (s-TTV). To characterize the genomic structure of these isolates in more detail, the full-length nucleotide sequence of the s-TTV isolate (designated s-TTV CH65-1), recovered from a chimpanzee born in West Africa, was ampl...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2000.0621
更新日期:2000-11-25 00:00:00
abstract::The complete RNA sequence of Sabin 3 (LED3) used in vaccine in the United States has been determined. The LED3 Sabin 3 sequence contains the attenuating mutations at bases 472 and 2034 but differs from that published by Stanway et al. (Nucleic Acids Res., 11, 5629-5643, 1983) at two other base positions, 2493 and 6061...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90576-w
更新日期:1991-12-01 00:00:00