Molecular characterization of Equine Infectious Anemia Viruses using targeted sequence enrichment and next generation sequencing.

abstract：:Trans-acting regulatory components of Autographa californica nuclear polyhedrosis virus (AcMNPV) were studied in a transient assay system for their ability to activate the p143 gene promoter. A region including 502 nt upstream of the AUG of the p143 gene was linked to the bacterial chloramphenicol acetyltransferase (C...

journal_title：Virology

authors： Lu A,Carstens EB

更新日期：1993-08-01 00:00:00

abstract：:Commelina yellow mottle virus (CoYMV) is the type member of the badnaviruses, a genus of plant pararetroviruses. The N-terminus of the polyprotein encoded by ORF III has limited similarity to known cell-to-cell movement proteins. To test the hypothesis that the N-terminus is required for viral movement, the phenotypes...

journal_title：Virology

authors： Tzafrir I,Ayala-Navarrete L,Lockhart BE,Olszewski NE

更新日期：1997-06-09 00:00:00

abstract：:Phase I human clinical studies involving therapeutics for emerging and biodefense pathogens with low incidence, such as the severe acute respiratory syndrome coronavirus (SARS-CoV), requires at a minimum preclinical evaluation of efficacy in two well-characterized and robust animal models. Thus, a ferret SARS-CoV mode...

journal_title：Virology

authors： Chu YK,Ali GD,Jia F,Li Q,Kelvin D,Couch RC,Harrod KS,Hutt JA,Cameron C,Weiss SR,Jonsson CB

更新日期：2008-04-25 00:00:00

abstract：:The complete RNA sequence of Sabin 3 (LED3) used in vaccine in the United States has been determined. The LED3 Sabin 3 sequence contains the attenuating mutations at bases 472 and 2034 but differs from that published by Stanway et al. (Nucleic Acids Res., 11, 5629-5643, 1983) at two other base positions, 2493 and 6061...

journal_title：Virology

authors： Weeks-Levy C,Tatem JM,DiMichele SJ,Waterfield W,Georgiu AF,Mento SJ

更新日期：1991-12-01 00:00:00

abstract：:Mutants of the influenza virus A/seal/Mass/1/80 (H7N7) are described which contain an insertion of 60 nucleotides in the hemagglutinin (HA) gene, derived most probably by recombination between the HA gene and the nucleoprotein gene of the same virus. The nonhomologous RNA recombination resulted in an enhanced hemagglu...

journal_title：Virology

authors： Orlich M,Gottwald H,Rott R

更新日期：1994-10-01 00:00:00

abstract：:An open reading frame which encodes at least 90% of the adenovirus type 2 DNA polymerase gene was cloned behind the SP6 promoter and transcribed in vitro using the SP6 RNA polymerase. The resultant RNA was translated in a rabbit reticulocyte cell free system. In addition to the translation of a 120-kDa protein corresp...

journal_title：Virology

authors： Hassin D,Korn R,Horwitz MS

更新日期：1986-11-01 00:00:00

abstract：:The technique of cDNA polymerase chain reaction was used to study the heterogeneity of adenovirus type 12 early region 1A mRNAs in infected and transformed cells. In addition to 13 S and 12 S mRNAs, three transcripts of 10 S, 9.5 S, and 9 S could be isolated from infected HeLa cells. A fourth transcript of 11 S was fo...

journal_title：Virology

authors： Brockmann D,Tries B,Esche H

更新日期：1990-12-01 00:00:00

abstract：:The group B noncultivatable rotavirus, ADRV, was purified from infected stool specimens. Double-stranded RNA was extracted, polyadenylated, reverse transcribed into cDNA, and cloned into plasmid vector pAT153. Each cDNA clone hybridized to a single ADRV RNA segment and cDNA clones of each genomic RNA segment were iden...

journal_title：Virology

authors： Chen GM,Hung T,Mackow ER

更新日期：1990-04-01 00:00:00

abstract：:Insertion of nucleotide sequences encoding "tags" that can be expressed in specific viral proteins during an infection is a useful strategy for purifying viral proteins and their functional complexes from infected cells and/or for visualizing the dynamics of their subcellular location over time. To identify regions in...

journal_title：Virology

authors： Teterina NL,Lauber C,Jensen KS,Levenson EA,Gorbalenya AE,Ehrenfeld E

更新日期：2011-01-05 00:00:00

abstract：:We examined the native plant host Solanum nigrum as reservoir of genetic diversity of begomoviruses that cause the tomato yellow leaf curl disease (TYLCD) emerging in southern Spain. Presence of isolates of all the species and strains found associated with TYLCD in this area was demonstrated. Mixed infections were com...

journal_title：Virology

authors： García-Andrés S,Monci F,Navas-Castillo J,Moriones E

更新日期：2006-07-05 00:00:00

abstract：:Like human adenovirus type 5 (HAV5), bovine adenovirus type 3 (BAV3) early region 1 (E1) consists of E1A and E1B transcriptional units. In order to characterize BAV3 E1 proteins and to isolate a cell line of bovine origin that expresses BAV3 E1, polyclonal antibodies specific to E1A, E1B-157R, and E1B-420R were raised...

journal_title：Virology

authors： van Olphen AL,Tikoo SK,Mittal SK

更新日期：2002-03-30 00:00:00

abstract：:The genome of the Bacillus subtilis phage SP01 contains a 12.6-kilobase-pair terminal redundancy. The transcription units in this region, which are utilized in vitro by B. subtilis RNA polymerase holoenzme, have been mapped. In vitro transcripts were separated and characterized by gel electrophoresis. Since there is s...

journal_title：Virology

authors： Brennan SM,Chelm BK,Romeo JM,Geiduschek EP

更新日期：1981-06-01 00:00:00

abstract：:Epstein-Barr virus (EBV) is a herpesvirus that transforms B-cells (B-LCL) and has undergone intense scrutiny owing to its association with Burkitt's lymphoma, nasopharyngeal carcinoma, and immunoblastic lymphomas. B-LCL have also proven useful in the study of human immunology. We describe a novel system for inducing e...

journal_title：Virology

authors： Curiel TJ,Cook DR,Bogedain C,Jilg W,Harrison GS,Cotten M,Curiel DT,Wagner E

更新日期：1994-02-01 00:00:00

abstract：:Tetherin (ST2/CD317) is a cellular protein that restricts the release from cells of some enveloped viruses including HIV-1. To examine if influenza virus is affected by tetherin, MDCK cells constitutively expressing human tetherin and control MDCK cells were infected with influenza virus. No difference was observed in...

journal_title：Virology

authors： Watanabe R,Leser GP,Lamb RA

更新日期：2011-08-15 00:00:00

abstract：:The biological significance of protective CD8 T-cell-mediated responses against non-traditional alternative reading frame epitopes remains relatively unknown. Cytolytic CD8 T cells (CTL) specific for a non-traditional cryptic MHC class I epitope, SYNTGRFPPL, are critically involved in the protection of mice during inf...

journal_title：Virology

authors： Rutkowski MR,Ho O,Green WR

更新日期：2009-08-01 00:00:00

abstract：:Six mutants of lambda having reduced dependence on the htpR function of Escherichia coli were isolated from lambda cIts857. Burst sizes in htpRts cells at 40.5 degrees were in the range of 10 to 20 particles per cell. Mapping and complementation analysis of one of the mutants suggested that the mutation in this isolat...

journal_title：Virology

authors： Fuerst CR

更新日期：1986-02-01 00:00:00

abstract：:Trichomonas vaginalis viruses (TVV), which may regulate P270 gene expression in the protozoan pathogen T. vaginalis, are a group of divergent double-stranded (ds) RNA viruses. In the present study, the complete 4674-bp cDNA sequence of a 4.6-kb ds RNA from a newly identified TVV2-1 isolate was determined. The sequence...

journal_title：Virology

authors： Bessarab IN,Liu HW,Ip CF,Tai JH

更新日期：2000-02-15 00:00:00

abstract：:Infectious bronchitis has remained one of the most difficult to control diseases in poultry since it was first described in 1931. Previous studies demonstrated that primary CD8(+) T lymphocytes collected at 10 days post-infection (p.i.) are important in controlling acute infection. To further investigate the role of m...

journal_title：Virology

authors： Pei J,Briles WE,Collisson EW

更新日期：2003-02-15 00:00:00

abstract：:Seven structural component proteins of a paramyxovirus simian virus 41 (SV41) were identified with the aid of monoclonal antibodies prepared against SV41 and human parainfluenza type 2 virus (PIV2). The nucleoprotein is antigenically very close to that of PIV2, while it is comparatively far from that of simian virus 5...

journal_title：Virology

authors： Tsurudome M,Bando H,Nishio M,Iwamoto Y,Kawano M,Kondo K,Komada H,Ito Y

更新日期：1990-12-01 00:00:00

abstract：:Viruses in certain genera in family Caulimoviridae were shown to integrate their genomic sequences into their host genomes and exist as endogenous pararetroviral sequences (EPRV). However, members of the genus Caulimovirus remained to be the exception and are known to exist only as episomal elements in the infected ce...

journal_title：Virology

authors： Pahalawatta V,Druffel K,Pappu H

更新日期：2008-07-05 00:00:00

abstract：:Open reading frame 89 (ORF89) is one of the three genes that are believed to be involved in the latent infection of white spot syndrome virus (WSSV). Here, we report the structure and functional characterization of ORF89. cDNA sequencing, 5' RLM-RACE, and 3' RLM-RACE showed that ORF89 gene is transcribed into an unspl...

journal_title：Virology

authors： Hossain MS,Khadijah S,Kwang J

更新日期：2004-07-20 00:00:00

abstract：:The DNA polymerase (DNApol) and major capsid protein (MCP) genes were used as models to study promoter activity in Chilo iridescent virus (CIV). Infection of Bombyx mori SPC-BM-36 cells in the presence of inhibitors of DNA or protein synthesis showed that DNApol, as well as helicase, is an immediate-early gene and con...

journal_title：Virology

authors： Nalçacioğlu R,Marks H,Vlak JM,Demirbaĝ Z,van Oers MM

更新日期：2003-12-20 00:00:00

abstract：:The DNA extracted from bacteriophage 643, which infects group N strains of Streptococci, could be separated into four components by electrophoresis on agarose gels. Electron microscopy established the predominant form of the DNA to be a relaxed, circular molecule of molecular weight 14.9 X 10(6). The four species obse...

journal_title：Virology

authors： Lyttle DJ,Petersen GB

更新日期：1984-03-01 00:00:00

abstract：:Monoclonal antibodies specific for eight antigenic sites on the G1 glycoprotein of La Crosse virus were studied in a kinetics of neutralization assay. When examined at the same protein concentration, there were differences in the rates and degree of neutralization induced by these antibodies. The maximum amount of neu...

journal_title：Virology

authors： Kingsford L

更新日期：1984-07-30 00:00:00

abstract：:Herpes simplex virus type 1 (HSV-1) glycoproteins K and B (gK and gB) are intimately involved in virus-induced fusion of cells. Certain mutations in the UL27 (gB) and UL53 (gK) genes confer a syncytial (syn) phenotype characterized by extensive fusion of infected cells and giving rise to multinucleated cells. We have ...

journal_title：Virology

authors： Hutchinson L,Graham FL,Cai W,Debroy C,Person S,Johnson DC

更新日期：1993-10-01 00:00:00

abstract：:Gibbon ape leukemia virus (GaLV) is a highly oncogenic C-type retrovirus capable of inducing myeloid leukemia in juvenile gibbons. GaLV is antigenically most closely related to a new world monkey virus, simian sarcoma associated virus (SSAV), and less to the murine and feline C-type leukemia viruses. To begin to under...

journal_title：Virology

authors： Delassus S,Sonigo P,Wain-Hobson S

更新日期：1989-11-01 00:00:00

abstract：:Six deletion mutations and an insertion were generated in the env gene of cloned copies of Moloney murine leukemia virus DNA. All seven mutants were replication-defective as tested by transformation of NIH/3T3 cells. The mutant DNAs were introduced into NIH/3T3 cells to generate stable producer lines; all released vir...

journal_title：Virology

authors： Granowitz C,Colicelli J,Goff SP

更新日期：1991-08-01 00:00:00

abstract：:The proteins encoded by open reading frame 3 (ORF3) of the umbraviruses pea enation mosaic virus-2 and tobacco mottle virus, like that of groundnut rosette virus, mediated the movement of viral RNA through the phloem of infected Nicotiana benthamiana or N. clevelandii plants when they were expressed from chimeric toba...

journal_title：Virology

authors： Ryabov EV,Robinson DJ,Taliansky M

更新日期：2001-09-30 00:00:00

abstract：:Retroviral nucleocapsid (NC) proteins are small Gag-derived products containing one or two zinc finger motifs that mediate genomic RNA packaging into virions. In this study, we addressed the role of the feline immunodeficiency virus (FIV) NC protein in the late stages of virus replication by analyzing the assembly phe...

journal_title：Virology

authors： Manrique ML,Rauddi ML,González SA,Affranchino JL

更新日期：2004-09-15 00:00:00

abstract：:NS35, a basic protein encoded by gene 8 of SA11 rotavirus, possesses RNA-binding activity and is essential for genome replication. To identify conserved regions in the NS35 gene and its protein product, we determined the nucleotide sequences of the NS35 gene for the mammalian and avian rotaviruses Wa, DS1, SA11 (Patto...

journal_title：Virology

authors： Patton JT,Salter-Cid L,Kalbach A,Mansell EA,Kattoura M

更新日期：1993-02-01 00:00:00