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Temporal and spatial expression of the E5a protein during the differentiation-dependent life cycle of human papillomavirus type 31b.

Abstract：

:Human papillomaviruses (HPVs) are epitheliotropic viruses, and their life cycle is intimately linked to the stratification and differentiation state of the host epithelial tissues. Defining a role for the E5 gene product in the differentiation-dependent viral life cycle has been difficult due to the lack of a suitable culture system. We used the organotypic (raft) culture system to investigate the spatial and temporal expression pattern of the E5 protein during the differentiation-dependent life cycle of HPV-31b. We report the generation of antisera specific to the HPV-31b E5a protein. The HPV-31b E5a protein was detected throughout the viral life cycle in raft cultures as determined by immunostaining analyses, and the protein was localized predominantly to the basal and granular layers. Expression of epidermal growth factor receptor or platelet-derived growth factor receptors, two proteins with which E5 has been shown to interact in cell culture, did not specifically colocalize with E5a expression. However, HPV-31b E5a expression did colocalize with the epithelial differentiation-specific marker filaggrin. The kinetics of E5a protein expression during the complete viral life cycle was analyzed by immunoblotting, and the highest level was found to be coincidental with the onset of virion morphogenesis.

journal_name

Virology

journal_title

Virology

authors

Mayer TJ,Meyers C

doi

10.1006/viro.1998.9262

subject

Has Abstract

pub_date

1998-09-01 00:00:00

pages

208-17

issue

2

eissn

0042-6822

issn

1096-0341

pii

S0042-6822(98)99262-5

journal_volume

248

pub_type

杂志文章

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