VP1u phospholipase activity is critical for infectivity of full-length parvovirus B19 genomic clones.

abstract：:Infection of eukaryotic cells in culture with herpes simplex virus type-1 (HSV-1) or HSV-2 increased the mutation frequency of the supF gene carried on the shuttle vector pZ189 by around sixfold. The increase was apparent 2 hr postinfection and reached a peak after 8 hr. To investigate this mutagenesis, plasmids pCKRR...

journal_title：Virology

authors： Clarke P,Clements JB

更新日期：1991-06-01 00:00:00

abstract：:In this paper we present a technique developed to physically locate the HSV-1 gene(s) which restore neurovirulence to a non-neurovirulent HSV intertypic recombinant described in the preceding report. In brief, tissue culture cells are co-transfected with unit length RE6 DNA and restriction endonuclease fragmented HSV-...

journal_title：Virology

authors： Thompson RL,Wagner EK,Stevens JG

更新日期：1983-11-01 00:00:00

abstract：:Virus-host interactions essential for alphavirus pathogenesis are poorly understood. To address this shortcoming, we coupled retrovirus insertional mutagenesis and a cell survival selection strategy to generate clonal cell lines broadly resistant to Sindbis virus (SINV) and other alphaviruses. Resistant cells had sign...

journal_title：Virology

authors： Kolokoltsova OA,Domina AM,Kolokoltsov AA,Davey RA,Weaver SC,Watowich SJ

更新日期：2008-07-20 00:00:00

abstract：:Sindbis virus (SINV) induces inflammatory and vasoactive responses that are associated with rash and arthritis in human infections. The mechanisms underlying infection-associated microvasculopathy are still unknown. We investigated whether endothelial cells infected by SINV are differentially responsive to bradykinin ...

journal_title：Virology

authors： Rust NM,Papa MP,Scovino AM,da Silva MM,Calzavara-Silva CE,Marques ET Jr,Peçanha LM,Scharfstein J,Arruda LB

更新日期：2012-01-05 00:00:00

abstract：:Mouse hepatitis virus (MHV) JHMV and its soluble receptor-resistant (srr) mutants, srr7, srr11, and srr18, grew and induced syncytia equally well in BHK-R1 cells expressing the MHVR1 receptor derived from MHV-susceptible BALB/c mice. In contrast, srr growth and syncytia formations were drastically reduced relative to ...

journal_title：Virology

authors： Matsuyama S,Taguchi F

更新日期：2000-07-20 00:00:00

abstract：:A cytopathic infectious agent was isolated from the kidneys of an apparently healthy tree shrew (Tupaia belangeri) that had been captured in the area around Bangkok. The infectivity was propagated in Tupaia fibroblast and kidney cell cultures. Paramyxovirus-like pleomorphic enveloped particles and helical nucleocapsid...

journal_title：Virology

authors： Tidona CA,Kurz HW,Gelderblom HR,Darai G

更新日期：1999-06-05 00:00:00

abstract：:The bovine papilloma virus (BPV) E1 protein essential to viral DNA replication has recently been shown to associate via direct protein-DNA interactions with the viral origin of replication and to be an ATP-dependent helicase. We show here that in accordance with the latter function, the E1 gene product has intrinsic A...

journal_title：Virology

authors： MacPherson P,Thorner L,Parker LM,Botchan M

更新日期：1994-10-01 00:00:00

abstract：:High levels of nonfused chloramphenicol acetyltransferase, beta-galactosidase, and beta-glucuronidase expressed under the control of new vector constructs of the polyhedrin promoter in Spodoptera frugiperda cells infected with Autographa californica nuclear polyhedrosis virus were investigated by SDS-PAGE and RNA dot ...

journal_title：Virology

authors： Luckow VA,Summers MD

更新日期：1989-05-01 00:00:00

abstract：:Expression of adenosine deaminase acting on RNA1 (ADAR1) is driven by alternative promoters. Promoter PA, activated by interferon (IFN), produces transcripts that encode the inducible p150 ADAR1 protein, whereas PB specifies the constitutively expressed p110 protein. We show using Stat1(-/-), Stat2(-/-) and IRF9(-/-) ...

journal_title：Virology

authors： George CX,Samuel CE

更新日期：2015-11-01 00:00:00

abstract：:Assembly of the viral genome into virions is a critical process of the virus life cycle often defining the ability of the virus to move within the plant and to be transmitted horizontally to other plants. Closteroviridae virions are polar helical rods assembled primarily by a major coat protein, but with a related min...

journal_title：Virology

authors： Satyanarayana T,Gowda S,Mawassi M,Albiach-Martí MR,Ayllón MA,Robertson C,Garnsey SM,Dawson WO

更新日期：2000-12-05 00:00:00

abstract：:Morphological and molecular changes produced by Autographa californica nuclear polyhedrosis virus (AcMNPV) infection in a permissive cell line, IPLB-SF-21AE (SF-21), of Spodoptera frugiperda and a nonpermissive cell line, FPMI-CF-203 (CF-203), of Choristoneura fumiferana are described. CF-203 cells inoculated with AcM...

journal_title：Virology

authors： Palli SR,Caputo GF,Sohi SS,Brownwright AJ,Ladd TR,Cook BJ,Primavera M,Arif BM,Retnakaran A

更新日期：1996-08-01 00:00:00

abstract：:Hepatitis delta virus (HDV) encodes only one protein, the hepatitis delta antigen (HDAg). Two forms of HDAg, a large (27 kDa) and a small (24 kDa) one, participate in the various steps of HDV replication. To further understand the properties of HDAg, we have constructed recombinant baculoviruses and expressed both for...

journal_title：Virology

authors： Hwang SB,Lee CZ,Lai MM

更新日期：1992-09-01 00:00:00

abstract：:Following challenge with COPV (canine oral papillomavirus), DNA plasmids encoding COPV L1, E1 or E2 protein were delivered into oral mucosal and cutaneous sites in beagles using particle-mediated immunotherapeutic delivery (PMID). Two weeks post-challenge, a priming dose of 8 microg DNA was delivered followed by a boo...

journal_title：Virology

authors： Moore RA,Walcott S,White KL,Anderson DM,Jain S,Lloyd A,Topley P,Thomsen L,Gough GW,Stanley MA

更新日期：2003-09-30 00:00:00

abstract：:The nucleotide sequences of the long terminal repeat (LTR) of simian retroviruses (STLV), which are closely related to human T-cell leukemia virus type I (HTLV-I) were found to be of at least two subgroups: an Asian subtype in macaques and an African subtype in African green monkeys and chimpanzee. The nucleotide sequ...

journal_title：Virology

authors： Watanabe T,Seiki M,Hirayama Y,Yoshida M

更新日期：1986-01-30 00:00:00

abstract：:Little is known about the neutralization properties of HIV-1 in India to optimally design and test vaccines. For this reason, a functional Env clone was obtained from each of ten newly acquired, heterosexually transmitted HIV-1 infections in Pune, Maharashtra. These clones formed a phylogenetically distinct genetic li...

journal_title：Virology

authors： Kulkarni SS,Lapedes A,Tang H,Gnanakaran S,Daniels MG,Zhang M,Bhattacharya T,Li M,Polonis VR,McCutchan FE,Morris L,Ellenberger D,Butera ST,Bollinger RC,Korber BT,Paranjape RS,Montefiori DC

更新日期：2009-03-15 00:00:00

abstract：:It has previously been shown that the genome of RF virus, a variant of the human papovavirus, BK, consists of two DNA species, one (R1a) with a deletion corresponding to the early and the other (R2) with a deletion corresponding to the late region of BKV (A. Pater, M.M. Pater, and G. di Mayorca (1980). J. Virol. 36, 4...

journal_title：Virology

authors： Pater A,Pater MM,Chang LS,Slawin K,Di Mayorca G

更新日期：1983-12-01 00:00:00

abstract：:Why some viruses establish chronic infections while others do not is poorly understood. One possibility is that the host's immune response is impaired during chronic infections and is unable to clear the virus from the host. In this report, we use a recently proposed framework to estimate the per capita killing effica...

journal_title：Virology

authors： Ganusov VV,Lukacher AE,Byers AM

更新日期：2010-09-15 00:00:00

abstract：:The physical stability of virions of Cucumber mosaic virus (CMV) mutants was investigated to determine if relative stability correlated with efficiency of aphid transmission. Virion stability was evaluated by a urea disruption assay and by testing the infectivity of virus following purifications. All viruses were infe...

journal_title：Virology

authors： Ng JC,Josefsson C,Clark AJ,Franz AW,Perry KL

更新日期：2005-02-05 00:00:00

abstract：:Soluble RNA-dependent RNA polymerases from healthy and alfalfa mosaic virus (AMV)-infected leaves were purified more than 400-fold from the 100,000g supernatant of leaf homogenates, using ammonium sulfate precipitation, Sephadex G-100 filtration, and chromatography on DEAE Sephadex A25 and P11-phosphocellulose. The DE...

journal_title：Virology

authors： Chifflot S,Sommer P,Hartmann D,Stussi-Garaud C,Hirth L

更新日期：1980-01-15 00:00:00

abstract：:The immediate-early transcripts of pseudorabies virus have been located in a region of the genome situated internally within the inverted repeat between map positions 0.99 and 0.95. A single immediate-early transcript (approximately 6 kb) can be detected both in the cytoplasmic and nuclear fractions of infected, cyclo...

journal_title：Virology

authors： Ihara S,Feldman L,Watanabe S,Ben-Porat T

更新日期：1983-12-01 00:00:00

abstract：:The HIV-1 accessory gene product Vpr can inhibit cell proliferation via cell cycle arrest at the G(2) phase, and it can induce apoptosis after G(2) arrest. We found recently that C81, a carboxy-terminally truncated form of Vpr, induced apoptosis via G(1) arrest but did not induce G(2) arrest of the cell cycle. Thus, i...

journal_title：Virology

authors： Nishizawa M,Kamata M,Mojin T,Nakai Y,Aida Y

更新日期：2000-10-10 00:00:00

abstract：:The helix-destabilizing properties of a single-stranded DNA-binding protein, LEF-3, of Bombyx mori nucleopolyhedrovirus (BmNPV) were studied. Partial duplexes of DNA containing single-stranded (ss) tails of different sizes and orientations were used as substrates for assay of the unwinding ability of LEF-3. Upon nonco...

journal_title：Virology

authors： Mikhailov VS

更新日期：2000-04-25 00:00:00

abstract：:The Epstein-Barr virus nuclear antigen 2A (EBNA-2A) has been strongly implicated in the EBV-mediated B-cell transformation process. Since EBNA-2A might exert this function through interaction with proteins of the infected cell, we studied the association of EBNA-2A with cellular proteins. Immunoprecipitation of EBNA-2...

journal_title：Virology

authors： Grässer FA,Sauder C,Haiss P,Hille A,König S,Göttel S,Kremmer E,Leinenbach HP,Zeppezauer M,Mueller-Lantzsch N

更新日期：1993-08-01 00:00:00

abstract：:The prevalence of hepatitis B virus vaccine escape mutants has increased as a consequence of the introduction of global vaccination programs. Furthermore and as a consequence of the organization of the genome of hepatitis B virus (HBV) into overlapping reading frames, the selection of polymerase mutants during long-te...

journal_title：Virology

authors： Torresi J,Earnest-Silveira L,Deliyannis G,Edgtton K,Zhuang H,Locarnini SA,Fyfe J,Sozzi T,Jackson DC

更新日期：2002-02-15 00:00:00

abstract：:Segment 7 (M) of the cold-adapted live influenza A virus vaccine plays a primary role in the ability of this virus to interfere with the replication of wild-type influenza A viruses. This conclusion is based on several lines of evidence. Single gene reassortant viruses derived by crossing influenza A/Ann Arbor/6/60 (H...

journal_title：Virology

authors： Whitaker-Dowling P,Zvolenski R,Youngner JS

更新日期：1991-01-01 00:00:00

abstract：:We previously demonstrated that cellular mRNAs are degraded in CD4 positive lymphocytes infected by the human immunodeficiency virus, HIV-1, but not in cells infected by the simian lentivirus, SIV. To begin to define the molecular mechanisms underlying this RNA degradation, we have established an in vitro RNA degradat...

journal_title：Virology

authors： Agy MB,Sherbert CH,Katze MG

更新日期：1996-03-01 00:00:00

abstract：:NF-kappaB comprises a family of transcription factors that regulate key immune processes. In this study, the effects of orthopoxvirus infection upon the activation of NF-kappaB were examined. During the early phase of infection, cowpox virus can inhibit the induction of NF-kappaB-regulated gene expression by interferi...

journal_title：Virology

authors： Oie KL,Pickup DJ

更新日期：2001-09-15 00:00:00

abstract：:The requirement for the herpes simplex virus type 1 (HSV-1) protein Vmw65 (VP16) for activation of immediate early (IE) gene expression was examined in synchronized HeLa cells. Analyses of IE RNA levels were conducted during infection with a viral Vmw65 mutant, in1814. The results revealed an increased requirement for...

journal_title：Virology

authors： Daksis JI,Preston CM

更新日期：1992-07-01 00:00:00

abstract：:The hemagglutinin of the 2009 pandemic H1N1 influenza virus is a derivative of and is antigenically related to classical swine but not to seasonal human H1N1 viruses. We compared the A/California/7/2009 (CA/7/09) virus recommended by the WHO as the reference virus for vaccine development, with two classical swine infl...

journal_title：Virology

authors： Min JY,Chen GL,Santos C,Lamirande EW,Matsuoka Y,Subbarao K

更新日期：2010-12-05 00:00:00

abstract：:The 64K surface protein of budded Autographa californica nuclear polyhedrosis virus (AcMNPV BV) is known to play a role in the functional entry of AcMNPV BV into Spodoptera frugiperda IPLB-SF-21 cells by adsorptive endocytosis. AcV1, a neutralizing monoclonal antibody, reacts with the 64K protein and in doing so preve...

journal_title：Virology

authors： Volkman LE,Goldsmith PA

更新日期：1988-09-01 00:00:00