Abstract:
:Sindbis virus (SINV) induces inflammatory and vasoactive responses that are associated with rash and arthritis in human infections. The mechanisms underlying infection-associated microvasculopathy are still unknown. We investigated whether endothelial cells infected by SINV are differentially responsive to bradykinin (BK), a potent inducer of inflammatory edema in a broad range of infectious diseases. Human endothelial cells (HBMECs) infected with SINV presented an upregulation of bradykinin B2 receptors (BK2R) expression. Also, BK reduced SINV-induced apoptosis and enhanced virus replication in HBMECs in a way dependent on BK2R, PI3 kinase and ERK signaling. Strikingly, intracerebral infection of mice in the presence of a BK2R antagonist reduced the local viral load. Our data suggest that SINV infection renders human endothelial cells hypersensitive to BK, which increases host cell survival and viral replication. Ongoing studies may clarify if the deregulation of the kinin pathway contributes to infection-associated vasculopathies in life-threatening arbovirus infections.
journal_name
Virologyjournal_title
Virologyauthors
Rust NM,Papa MP,Scovino AM,da Silva MM,Calzavara-Silva CE,Marques ET Jr,Peçanha LM,Scharfstein J,Arruda LBdoi
10.1016/j.virol.2011.10.003subject
Has Abstractpub_date
2012-01-05 00:00:00pages
81-91issue
1eissn
0042-6822issn
1096-0341pii
S0042-6822(11)00471-5journal_volume
422pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::The transmembrane envelope (TM) proteins of retroviruses are used as antigen in diagnostic immunoassays and they represent a conserved target for neutralizing antibodies. To analyze the situation in infections with the feline foamy virus (FFV), its recombinant TM protein was produced and used for ELISA and Western blo...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2011.01.023
更新日期:2011-04-10 00:00:00
abstract::Attempts to define the genetic determinants required for efficient growth of human immunodeficiency virus type 1 (HIV-1) in monocyte-macrophages were made by constructing chimeras between two infectious clones of HIV-1 (HXB2 and LW/C), which despite only minor differences in their DNA sequence have striking difference...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(95)80082-4
更新日期:1995-01-10 00:00:00
abstract::While DNA vaccines have been shown to prime cellular immune responses, levels are often low in nonhuman primates or humans. Hence, efforts have been directed toward boosting responses by combining DNA with different vaccination modalities. To this end, a polyvalent DNA prime/protein boost vaccine, consisting of codon ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.10.038
更新日期:2006-03-01 00:00:00
abstract::Using reverse genetics methods, we constructed three different transfectant influenza A viruses encoding an Ld-restricted, nine amino-acid-long fragment, corresponding to amino-acid residues 876-884, of beta-galactosidase (beta-gal). Sequences encoding this epitope were nested within the hemagglutinin (HA) or neuramin...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9330
更新日期:1998-09-15 00:00:00
abstract::Sequence variants of the Epstein-Barr virus (EBV)-encoded latent membrane protein-1 (LMP1) have been reported in association with EBV-linked malignancies but little is known about their effects on signalling pathways and phenotype. We have examined the ability of the nasopharyngeal carcinoma (NPC)-derived variant, CAO...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2001.0828
更新日期:2001-04-10 00:00:00
abstract::The v-erb B protein of avian erythroblastosis virus (AEV) possesses an associated protein kinase activity in vitro. Analysis of temperature-sensitive mutants, and nonconditional host range mutants of AEV demonstrated that there was no simple correlation between this autophosphorylation activity and the transformation ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90287-4
更新日期:1986-04-15 00:00:00
abstract::The surface protein of Marburg virus (GP) is modified by acylation, as shown by labeling with [3H]myristic and [3H]palmitic acid. Acylation of GP also occurred when it was expressed in insect cells with the baculovirus expression system. Gas chromatographic analyses of the bound fatty acids indicated that exogenously ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.1151
更新日期:1995-04-01 00:00:00
abstract::Hepatitis delta virus (HDV) encodes only one protein, the hepatitis delta antigen (HDAg). Two forms of HDAg, a large (27 kDa) and a small (24 kDa) one, participate in the various steps of HDV replication. To further understand the properties of HDAg, we have constructed recombinant baculoviruses and expressed both for...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)91227-l
更新日期:1992-09-01 00:00:00
abstract::Sequences critical for the activity of the measles virus (MV) RNA polymerase in transcription and replication were analyzed using a MV genomic cDNA library containing overlapping clones encompassing the entire MV genome. Clones corresponding to the 3' and 5' ends of the MV genome were identified and sequenced, and the...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90564-8
更新日期:1988-06-01 00:00:00
abstract::At high concentrations, several monoclonal antibodies to the G1 glycoprotein of La Crosse (LAC) virus aggregated the virus. To determine whether this accounted for the neutralization, the monoclonal antibodies were digested to make Fab fragments. With one exception, each monovalent antibody neutralized LAC virus to th...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90073-k
更新日期:1991-02-01 00:00:00
abstract::Myeloid differential primary response protein (MyD88) is a critical component in the signaling cascade through Toll-like receptors (TLRs) and is induced by alpha interferon (IFN-alpha). To examine the role of MyD88 in the antiviral activity of IFN-alpha against hepatitis B virus (HBV), we established MyD88 stably expr...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2003.11.005
更新日期:2004-02-20 00:00:00
abstract::The genomes of human herpes virus type-1 and type-2 share a high degree of sequence identity; yet, they exhibit important differences in pathology in their natural human host as well as in animal host and cell cultures. Here, we report the comparative analysis of the time and relative abundance profiles of the transcr...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.12.036
更新日期:2006-04-25 00:00:00
abstract::The uncoating of tobacco mosaic virus (TMV) particles in protoplasts isolated from leaves of transgenic tobacco plants that express the TMV coat protein gene was investigated. Extracts of these protoplasts collected up to 1 hr after inoculation with TMV contained fewer of the complexes ("striposomes") thought to be in...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90163-l
更新日期:1990-12-01 00:00:00
abstract::Synthesis of unintegrated retroviral DNA via reverse transcription is thought to involve two separate template switches. Minus-strand strong-stop DNA, a major product of in vitro reactions using detergent-treated virions, is synthesized prior to the first template switch. Plus-strand strong-stop DNA, which is found ba...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1237
更新日期:1993-05-01 00:00:00
abstract::The red clover necrotic mosaic dianthovirus (RCNMV) genome is split between two single-stranded RNA species. The polycistronic RNA-1 encodes the viral RNA polymerase and capsid protein (CP) and the monocistronic RNA-2 encodes the 35 kDa cell-to-cell movement protein (MP). Nicotiana benthamiana plants transformed with ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.1518
更新日期:1995-10-01 00:00:00
abstract::Severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) caused a severe outbreak in several regions of the world in 2003. The SARS-CoV genome is predicted to contain 14 functional open reading frames (ORFs). The first ORF (1a and 1b) encodes a large polyprotein that is cleaved into nonstructural proteins (nsp)...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2007.04.029
更新日期:2007-09-30 00:00:00
abstract::Progressive Multifocal Leukoencephalopathy (PML) is caused by lytic replication of JC virus (JCV) in specific cells of the central nervous system. Like other polyomaviruses, JCV encodes a large T-antigen helicase needed for replication of the viral DNA. Here, we report the development of a luciferase-based, quantitati...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2014.07.042
更新日期:2014-11-01 00:00:00
abstract::Immunoelectron microscopy using an anti-VP2 monoclonal antibody complexed to colloidal gold has been used to study the mechanism of bluetongue virus (BTV) release from infected cells. Examination of the BTV-infected cell surface revealed that viruses are released both as enveloped particles, by budding through the pla...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90218-3
更新日期:1989-11-01 00:00:00
abstract::Dengue viruses (DENV) are the most important arboviral pathogens in tropical and subtropical regions throughout the world. DENV transmission includes both a sylvatic, enzootic cycle between nonhuman primates and arboreal mosquitoes of the genus Aedes, and an urban, endemic/epidemic cycle between Aedes aegypti, a mosqu...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.08.049
更新日期:2007-02-20 00:00:00
abstract::The DNA extracted from bacteriophage 643, which infects group N strains of Streptococci, could be separated into four components by electrophoresis on agarose gels. Electron microscopy established the predominant form of the DNA to be a relaxed, circular molecule of molecular weight 14.9 X 10(6). The four species obse...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90406-9
更新日期:1984-03-01 00:00:00
abstract::Gp110 of Epstein-Barr virus (EBV) mainly localizes on nuclear/ER membranes and plays a role in the assembly of EBV nucleocapsid. The C-terminal tail domain (gp110 CTD) is essential for the function of gp110 and the nuclear/ER membranes localization of gp110 is ruled by its C-terminal unique nuclear localization signal...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.06.031
更新日期:2008-09-30 00:00:00
abstract::Porcine epidemic diarrhea virus (PEDV) is a causative agent of porcine epidemic diarrhea (PED). PED, characterized by acute diarrhea, vomiting, dehydration, has caused serious economic losses in pig industry worldwide. Here, we demonstrate that activation of a disintergrin and metalloprotease 17 (ADAM17) induced the d...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2018.02.001
更新日期:2018-04-01 00:00:00
abstract::The Psu (Polarity suppression) protein of satellite bacteriophage P4 was first characterized as an anti-terminator of transcription termination in Escherichia coli. Psu is also a structural component of mature P4 capsids, where it is present as a decoration protein. Psu is located externally on the capsid surface, and...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1307
更新日期:1993-06-01 00:00:00
abstract::Begomoviruses (family Geminiviridae) are responsible for many economically important crop diseases worldwide. The majority of these diseases are caused by bipartite begomovirus infections, although a rapidly growing number of diseases of the Old World are associated with monopartite begomoviruses. With the exception o...
journal_title:Virology
pub_type: 杂志文章,随机对照试验,评审
doi:10.1016/j.virol.2005.09.042
更新日期:2006-01-05 00:00:00
abstract::Cells that express the human papillomavirus (HPV) type 16 E7 oncoprotein are predisposed to undergo apoptosis. Transgenic mice that have E7 expression targeted to either the retinal photoreceptor cells or the lens cells exhibit signs of apoptosis in cells attempting to undergo differentiation. We established a cell cu...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8851
更新日期:1997-12-08 00:00:00
abstract::Replication of Tomato bushy stunt virus (TBSV) RNA takes place on the cytosolic membrane surface of peroxisomes in plants and in yeast, a model host. To identify the host proteins involved in assisting the peroxisomal localization of the tombusvirus p33 replication protein, we tested if p33 could bind directly to yeas...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.06.044
更新日期:2008-09-30 00:00:00
abstract::Economic loss due to viral endothelial cell necrosis of eel (VECNE) of Anguilla japonica is a serious problem for the cultured Japanese eel market. However, the viral genome responsible for VECNE is unknown. We recently developed a rapid determination system for viral nucleic acid sequences (RDV) to determine viral ge...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.12.057
更新日期:2011-03-30 00:00:00
abstract::A full-length cDNA of cucurbit aphid-borne yellows virus (CABYV) has been constructed and expressed either as an in vitro transcript, under control of a bacteriophage T7 RNA polymerase promoter, or in vivo, under control of the cauliflower mosaic virus 35S promoter in an agroinfection vector. The biological activity o...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.9945
更新日期:1995-12-01 00:00:00
abstract::Ts1, a temperature-sensitive mutant of Moloney murine leukemia virus-TB (MoMuLV-TB), causes a progressive hindlimb paralytic disease in susceptible strains of mice. Previously, it has been shown that a single amino acid substitution, Val-25----Ile in gPr80env, is responsible for the temperature sensitivity, inefficien...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90438-h
更新日期:1991-10-01 00:00:00
abstract:BACKGROUND:When antiretroviral therapy does not fully suppress HIV replication, suboptimal levels of antiretrovirals can select for antiretroviral resistant variants of HIV. These variants may exhibit reduced replication capacity and result in lower viral loads in blood. Our study evaluated whether antiretroviral resis...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.08.007
更新日期:2009-11-25 00:00:00