Abstract:
:Severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) caused a severe outbreak in several regions of the world in 2003. The SARS-CoV genome is predicted to contain 14 functional open reading frames (ORFs). The first ORF (1a and 1b) encodes a large polyprotein that is cleaved into nonstructural proteins (nsp). The other ORFs encode for four structural proteins (spike, membrane, nucleocapsid and envelope) as well as eight SARS-CoV-specific accessory proteins (3a, 3b, 6, 7a, 7b, 8a, 8b and 9b). In this report we have cloned the predicted nsp8 gene and the ORF6 gene of the SARS-CoV and studied their abilities to interact with each other. We expressed the two proteins as fusion proteins in the yeast two-hybrid system to demonstrate protein-protein interactions and tested the same using a yeast genetic cross. Further the strength of the interaction was measured by challenging growth of the positive interaction clones on increasing gradients of 2-amino trizole. The interaction was then verified by expressing both proteins separately in-vitro in a coupled-transcription translation system and by coimmunoprecipitation in mammalian cells. Finally, colocalization experiments were performed in SARS-CoV infected Vero E6 mammalian cells to confirm the nsp8-ORF6 interaction. To the best of our knowledge, this is the first report of the interaction between a SARS-CoV accessory protein and nsp8 and our findings suggest that ORF6 protein may play a role in virus replication.
journal_name
Virologyjournal_title
Virologyauthors
Kumar P,Gunalan V,Liu B,Chow VT,Druce J,Birch C,Catton M,Fielding BC,Tan YJ,Lal SKdoi
10.1016/j.virol.2007.04.029subject
Has Abstractpub_date
2007-09-30 00:00:00pages
293-303issue
2eissn
0042-6822issn
1096-0341pii
S0042-6822(07)00314-5journal_volume
366pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::Although dengue (DEN) virus is the etiologic agent of dengue fever, the most prevalent vector-borne viral disease in the world, precise information on the antigenic structure of the dengue virion is limited. We have prepared a set of murine monoclonal antibodies (MAbs) specific for the envelope (E) glycoprotein of DEN...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9200
更新日期:1998-07-05 00:00:00
abstract::A series of nucleotide substitutions (G46----C; C47----A; C315----U; U317----C) were introduced into the virulence modulating region of the intermediate strain of potato spindle tuber viroid (PSTVd) in order to examine their effect upon viroid infectivity and pathogenicity with the presence of all four mutations resul...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90468-5
更新日期:1992-04-01 00:00:00
abstract::Previous serological studies of experimental infection with duck hepatitis B virus (DHBV) have shown that the outcome of infection depends largely on the age of the duck at the time of inoculation. To examine the hypothesis that decreased susceptibility with increased age might be due to the loss of the virus receptor...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90017-j
更新日期:1992-02-01 00:00:00
abstract::Latent membrane protein 2A (LMP2A) is expressed in latent Epstein-Barr virus (EBV) infection. LMP2A functions to downregulate B-cell signal transduction and viral reactivation from latency in EBV-immortalized B cells in vitro, and acts to provide B cells with both a survival and developmental signal in vivo. Identific...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1999.0166
更新日期:2000-03-01 00:00:00
abstract::Multiplication of frog virus 3 (FV 3) occurs in mammalian cells provided they are incubated at temperatures lower than 33 degrees. The expression of the viral genome at supraoptimal temperatures was followed by analyzing the polypeptides produced in CHO-infected cells and comparing with those obtained under restrictiv...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90138-8
更新日期:1986-07-30 00:00:00
abstract::Retroviruses can be grouped by viral interference measurements into classes which use common cell surface receptors. We previously tested a large number of isolates of mink cell focus-inducing (MCF) murine leukemia viruses (MuLVs), and reported that all of them share a distinct receptor on NIH/3T3 cells (A. Rein, Viro...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90255-1
更新日期:1984-07-15 00:00:00
abstract::The retroviral cyclin protein (rv-cyclin) of walleye dermal sarcoma virus contains two known functional domains, a cyclin box motif and a carboxy terminal transcription activation domain (AD). The AD contacts TATA-binding protein-associated factor 9 (TAF9), and this action is necessary for both positive and negative r...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.12.026
更新日期:2009-03-30 00:00:00
abstract::Using a defective interfering Sendai virus stock (DIH4) freed of nondefective helper virus, we found that the closely related parainfluenza viruses 1 and 3 could substitute for the Sendai virus helper in replicating DIH4, creating chimeric nucleocapsids. The morbillivirus measles and the rhabdovirus VSV could not subs...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90660-4
更新日期:1991-05-01 00:00:00
abstract::The Autographa californica nuclear polyhedrosis virus da26 gene codes for an envelope protein of both budded virus (BV) and occlusion derived virus (ODV). Western blot and temporal analysis of infected cell extracts detected a protein of 26 kDa by 4 h postinfection (p.i.). The amount of protein increased by 16 h p.i. ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8903
更新日期:1998-01-05 00:00:00
abstract::The nucleotide and deduced amino acid sequence of the gene 4 of murine rotavirus strain Eb were determined. The gene is 2359 nucleotides in length and encodes for a protein of 775 amino acids. Comparison of the VP4 amino acid sequence of the Eb strain with several human and animal rotavirus strains which represent all...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1153
更新日期:1994-03-01 00:00:00
abstract::Condylomata acuminata is caused by human papillomavirus (HPV). PCR with consensus primers will typically detect HPV in >96% of condylomata. Metagenomic sequencing has found that some "HPV-negative" condylomata do indeed contain HPV. We wished to perform a renewed evaluation of the "HPV-negative" condylomata using deep...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2015.07.023
更新日期:2015-11-01 00:00:00
abstract::A range of deletion and other mutants in the coding region of the E1A gene of Ad5 has been assayed for transformation of baby rat kidney (BRK) cells in cooperation with ras, repression of the SV40 enhancer, and induction of proliferating cell nuclear antigen (PCNA). Transformation efficiency was drastically reduced by...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90518-7
更新日期:1989-07-01 00:00:00
abstract::Nineteen independent temperature-sensitive (ts) mutants were isolated from SA11 following mutagenesis with proflavin or 5-azacytidine. Fourteen of the ts mutants fell into one or another of five mutant groups previously defined by recombination. Five of the ts mutants defined five recombination groups (F, G, H, I, and...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(83)90100-9
更新日期:1983-10-30 00:00:00
abstract::To determine the role of piscine anti-viral cytotoxic cells, we analyzed the response of channel catfish to Ictalurid herpesvirus 1, commonly designated channel catfish virus (CCV). Peripheral blood leukocytes (PBL) from catfish immunized with MHC-matched, CCV-infected G14D cells (G14D-CCV) showed marked lysis of G14D...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2019.11.014
更新日期:2020-01-15 00:00:00
abstract::We have mapped the genome of lettuce necrotic yellows virus (LNYV), the type member of the genus cytorhabdovirus of the family Rhabdoviridae. We have cloned and sequenced all intergenic regions and the 3' leader and 5' trailer of the negative-sense, single-stranded RNA genome of LNYV. The LNYV genome appears to contai...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1204
更新日期:1994-05-01 00:00:00
abstract::Outbreaks of avian influenza virus H5N8 first occurred in 2014, and spread to poultry farms in Korea. Although there was no report of human infection by this subtype, it has the potential to threaten human public health. Therefore, we evaluated the pathogenesis of H5N8 viruses in ferrets. Two representative Korean H5N...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2015.02.042
更新日期:2015-07-01 00:00:00
abstract::Few human endogenous retroviruses (HERVs) have been extensively studied in non-human primates. Such investigations have demonstrated that several element classes are primate unique, contain members with important biological function, are conserved in specific primate lineages, and have in some cases expanded in copy n...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.01.045
更新日期:2005-04-10 00:00:00
abstract::Two HLA-A*02-restricted epitopes have been identified within the VP1 polypeptide of a human polyomavirus, BK virus, which is associated with polyomavirus-associated nephropathy in kidney transplant patients. Immunization of transgenic mice with recombinant modified vaccinia Ankara expressing BKV VP1 (rMVA-BKV VP1) eli...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.02.040
更新日期:2006-06-20 00:00:00
abstract::Human infections with highly pathogenic H5N1 avian influenza viruses continue to occur in many parts of the world and pose a considerable public health threat. With the use of animal models, the identification of virulence determinants has been instrumental in improving our understanding of how these viruses cause sev...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2011.02.005
更新日期:2011-05-10 00:00:00
abstract::Hepatitis delta virus (HDV) encodes two proteins, the small hepatitis delta antigen (SHDAg) and large hepatitis delta antigen (LHDAg). Both proteins are identical except for the presence of additional 19 amino acids at the C terminus of LHDAg. While SHDAg is required for HDV RNA replication, LHDAg inhibits replication...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1109
更新日期:1994-02-15 00:00:00
abstract::The physical structure of the genome of feline herpesvirus-1, a major upper respiratory tract pathogen of cats, was studied. Purified FHV-1 DNA was analyzed by restriction endonuclease and gamma 5' exonuclease digestion, blot hybridization, and electron microscopy. To facilitate further studies, nine bacteriophage clo...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90439-3
更新日期:1986-10-15 00:00:00
abstract::Human immunodeficiency virus type 1 (HIV-1) transmembrane glycoprotein gp41 is targeted by broadly-reactive neutralizing antibodies 2F5 and 4E10, making it an attractive target for vaccine development. To better assess immunogenic properties of gp41, we generated five soluble glutathione S-transferase fusion proteins ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2007.11.009
更新日期:2008-03-15 00:00:00
abstract::Human cytomegalovirus (HCMV) lytic DNA replication is initiated at the cis-acting oriLyt region and requires six core replication proteins along with UL84 and IE2. Although UL84 is thought to be the replication initiator protein, little is known about its interaction with oriLyt. We have now performed chromatin immuno...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.06.035
更新日期:2009-09-15 00:00:00
abstract::The Crinivirus, Lettuce infectious yellows virus (LIYV) has a bipartite, positive-sense ssRNA genome. LIYV RNA 1 encodes replication-associated proteins while RNA 2 encodes proteins needed for other aspects of the LIYV life cycle. LIYV RNA 1 ORF 2 encodes P34, a trans enhancer for RNA 2 accumulation. Here we show that...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.04.006
更新日期:2010-07-20 00:00:00
abstract::Tandemers 2MG, 2MG3, 3MG and 4MG are derivatives of the potent anti-HIV-1 microbicide candidate griffithsin (GRFT). We compared these compounds anti-HIV-1 activity to GRFT using the viruses CAP206.08 and CAAN5342.A2 that have decreased sensitivity to this lectin. The 2MG and 2MG3 tandemers had similar activity to GRFT...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2020.01.017
更新日期:2020-05-01 00:00:00
abstract::Adeno-associated virus (AAV) replication proteins Rep78 and Rep68 play major roles in the life cycle of AAV. We have recently provided in vivo evidence for the existence of a covalent association between Rep78 and virion single-stranded (ss) AAV DNA (K. M. R. Prasad and J.P. Trempe(1995) Virology 214, 360-370). In thi...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.8431
更新日期:1997-03-03 00:00:00
abstract::RNA interference (RNAi) is the process by which double-stranded RNA (dsRNA) directs sequence-specific degradation of messenger RNA in animal and plant cells. In mammalian cells, RNAi can be triggered by 21-23 nucleotide duplexes of small interfering RNA (siRNA). Strategies to inhibit RNA virus multiplication based on ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.01.051
更新日期:2005-05-25 00:00:00
abstract::The role of the nucleocapsid protein of HIV-1 Gag in virus assembly was investigated using Gag truncation mutants, a nucleocapsid deletion mutant, and point mutations in the nucleocapsid region of Gag, in transfected COS cells, and in stable T-cell lines. Consistent with previous investigations, a truncation containin...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9374
更新日期:1998-11-10 00:00:00
abstract::Feline morbillivirus (FmoPV) has recently been identified in Hong Kong and Japan. FmoPV is considered to belong to the genus Morbillivirus, in the family Paramyxoviridae. In this study, the complete nucleotide sequences of three strains of FmoPV detected in cats in Japan were determined. Among the six genes in FmoPV; ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2014.09.003
更新日期:2014-11-01 00:00:00
abstract::Selective inactivation of critical cysteine residues in human immunodeficiency virus type one (HIV-1) was observed after treatment with 4-vinylpyridine (4-VP), with and without the membrane-permeable metal chelator N,N,N',N'-tetrakis(2-pyridylmethyl)-ethylenediamine (TPEN). Chromatographic analysis showed that cystein...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.01.045
更新日期:2008-05-25 00:00:00