Abstract:
:Porcine epidemic diarrhea virus (PEDV) is a causative agent of porcine epidemic diarrhea (PED). PED, characterized by acute diarrhea, vomiting, dehydration, has caused serious economic losses in pig industry worldwide. Here, we demonstrate that activation of a disintergrin and metalloprotease 17 (ADAM17) induced the decrease of PEDV infection in HEK293 and IPEC-J2 cells and the downregulation of cell surface aminopeptidase N (APN) expression, an important entry factor for PEDV infection. Furthermore, overexpression of ADAM17 suppressed PEDV infection in HEK293 and IPEC-J2 cells, whereas ablation of ADAM17 expression using ADAM17 specific siRNA resulted in a corresponding increase of PEDV infection and an upregulation of cell surface APN expression. Taken together, these data demonstrate that modulation of APN expression by metalloprotease ADAM17 regulates PEDV infection. Hence, the reduction in APN expression represents another component of the anti-PEDV infection response initiated by ADAM17.
journal_name
Virologyjournal_title
Virologyauthors
Zhang J,Guo L,Yang L,Xu J,Zhang L,Feng L,Chen H,Wang Ydoi
10.1016/j.virol.2018.02.001subject
Has Abstractpub_date
2018-04-01 00:00:00pages
24-29eissn
0042-6822issn
1096-0341pii
S0042-6822(18)30038-2journal_volume
517pub_type
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