Abstract:
:The protein content of vaccinia virus mature virions, purified by rate zonal and isopycnic centrifugations and solubilized by SDS or a solution of urea and thiourea, was determined by the accurate mass and time tag technology which uses both tandem mass spectrometry and Fourier transform-ion cyclotron resonance mass spectrometry to detect tryptic peptides separated by high-resolution liquid chromatography. Eighty vaccinia virus-encoded proteins representing 37% of the 218 genes annotated in the complete genome sequence were detected in at least three analyses. Ten proteins accounted for approximately 80% of the virion mass. Thirteen identified proteins were not previously reported as components of virions. On the other hand, 8 previously described virion proteins were not detected here, presumably due to technical reasons including small size and hydrophobicity. In addition to vaccinia virus-encoded proteins, 24 host proteins omitting isoforms were detected. The most abundant of these were cytoskeletal proteins, heat shock proteins and proteins involved in translation.
journal_name
Virologyjournal_title
Virologyauthors
Resch W,Hixson KK,Moore RJ,Lipton MS,Moss Bdoi
10.1016/j.virol.2006.08.025subject
Has Abstractpub_date
2007-02-05 00:00:00pages
233-47issue
1eissn
0042-6822issn
1096-0341pii
S0042-6822(06)00585-Xjournal_volume
358pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::Feline morbillivirus (FmoPV) has recently been identified in Hong Kong and Japan. FmoPV is considered to belong to the genus Morbillivirus, in the family Paramyxoviridae. In this study, the complete nucleotide sequences of three strains of FmoPV detected in cats in Japan were determined. Among the six genes in FmoPV; ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2014.09.003
更新日期:2014-11-01 00:00:00
abstract::Recent studies have shown that interferon-gamma (IFN-gamma) synergizes with IFN-alpha/beta to inhibit the replication of both RNA and DNA viruses. We investigated the effects of IFNs on the replication of two strains of severe acute respiratory syndrome-associated coronavirus (SARS-CoV). While treatment of Vero E6 cel...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2004.08.011
更新日期:2004-11-10 00:00:00
abstract::Autographa californica multiple nucleopolyhedrovirus (AcMNPV) encodes two proteins that possess properties typical of single-stranded DNA-binding proteins (SSBs), late expression factor-3 (LEF-3), and a protein referred to as DNA-binding protein (DBP). Whereas LEF-3 is a multi-functional protein essential for viral DN...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2007.03.024
更新日期:2007-08-01 00:00:00
abstract::The mouse mammary tumor virus (MMTV) is a type B retrovirus that is unique from other retroviruses in having multiple "tissue specific" and "hormone inducible" promoters. This unique feature has lead to the increasing interest in studying the biology of MMTV replication with the ultimate goal of developing MMTV based ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.12.027
更新日期:2009-03-15 00:00:00
abstract::Respiratory syncytial virus (RSV) induces the production of a number of cytokines and chemokines by activation of nuclear factor kappa B (NF-kappaB). The activation of NF-kappaB has been shown to depend on viral replication in the infected cells. In this study, we demonstrate that expression of RSV M2-1 protein, a tra...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2004.10.031
更新日期:2005-01-20 00:00:00
abstract::HK97 is a temperate dsDNA bacteriophage of Escherichia coli that is structurally similar to phage lambda, with an icosahedral head of triangulation (7) number 7. Although the capsids of several large dsDNA phages have been studied extensively using a variety of biophysical approaches, no high-resolution structure is a...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9040
更新日期:1998-03-30 00:00:00
abstract::Neutralizing monoclonal antibodies 2F1 and 1C10, which are specific for VP7 of serotype 2 rotaviruses (G2), were used to select neutralization escape variants of the human serotype 2 rotavirus, DS-1. Neutralization survival patterns generated by monoclonal antibodies 2F1, 1C10, and RV5:3 indicated that 2F1 and 1C10 di...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1601
更新日期:1993-11-01 00:00:00
abstract::Here we describe the complete genome sequences of two strains of Usutu virus (USUV), a mosquito-borne member of the genus Flavivirus in the Japanese encephalitis virus (JEV) serogroup. USUV was detected in Austria in 2001 causing a high mortality rate in blackbirds; the reference strain (SAAR-1776) was isolated in 195...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2004.08.005
更新日期:2004-10-25 00:00:00
abstract::We used a series of deletion mutations in the 5' untranslated region of the prototype D type retrovirus, Mason-Pfizer Monkey Virus (MPMV), to analyse RNA encapsidation. A region was identified upstream of the major splice donor which reduced particle production but had a proportionally greater effect on RNA packaging....
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2001.1061
更新日期:2001-09-15 00:00:00
abstract::To investigate differential evolutionary rates and selective forces of WNV in hosts and vectors, we measured the genetic diversity that arose during alternating passage in mosquitoes and birds. Within-host genetic diversity was monitored in each of three experimentally passed replicates, and the complete genome sequen...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.02.032
更新日期:2008-05-10 00:00:00
abstract::Our recent results demonstrated that bile acids facilitate virus escape from the endosomes into the cytoplasm for successful replication of porcine enteric calicivirus (PEC). We report a novel finding that bile acids can be substituted by cold treatment for endosomal escape and virus replication. This endosomal escape...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2015.04.022
更新日期:2015-09-01 00:00:00
abstract::Demyelination of the human peripheral nervous system (PNS) can be caused by diverse mechanisms including viral infection. Despite association of several viruses with the development of peripheral demyelination, animal models of the condition have been limited to disease that is either autoimmune or genetic in origin. ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.09.009
更新日期:2007-03-01 00:00:00
abstract::Varicella Zoster virus (VZV) productively infects humans causing varicella upon primary infection and herpes zoster upon reactivation from latency in neurons. In vitro studies using cell-associated VZV infection have demonstrated productive VZV-infection, while a few recent studies of human neurons derived from stem c...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2013.05.021
更新日期:2013-09-01 00:00:00
abstract::We recently showed that E6 protein of human papillomavirus (HPV) 16, a mucosal high-risk α-PV type, can potentiate Wnt/β-catenin/TCF signaling. Here we investigated the transcriptional activities of E6 proteins of cutaneous HPV types from the β and α genera. Results from reporter-gene assays showed that similar to HPV...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2017.05.020
更新日期:2017-09-01 00:00:00
abstract::Middle East respiratory syndrome coronavirus (MERS-CoV) infects host cells through binding the receptor binding domain (RBD) on its spike glycoprotein to human receptor dipeptidyl peptidase 4 (hDPP4). Here, we report identification of critical residues on hDPP4 for RBD binding and virus entry through analysis of a pan...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2014.10.006
更新日期:2014-12-01 00:00:00
abstract::To gain new insights regarding the role of Ku, the DNA-PK DNA-binding component, during lentiviral DNA integration, we have investigated the HIV-1 replication in Ku80-depleted human cells. CEM4fx cells underexpressing the Ku80 factor were selected after transduction by a retroviral vector expressing the Ku80 full-leng...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2002.1515
更新日期:2002-08-15 00:00:00
abstract::In this study, we present enzymatic differences found between recombinant RTs derived from feline leukemia virus and feline immunodeficiency virus. Firstly, FIV RT showed low steady state K(m) values for dNTPs compared to FeLV RT. Consistent with this, FIV RT synthesized DNA more efficiently than FeLV RT at low dNTP c...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.02.010
更新日期:2005-04-25 00:00:00
abstract::Virus genomes are condensed and packaged inside stable proteinaceous capsids that serve to protect them during transit from one cell or host organism, to the next. During virus entry, capsid shells are primed and disassembled in a complex, tightly-regulated, multi-step process termed uncoating. Here we compare the unc...
journal_title:Virology
pub_type: 杂志文章,评审
doi:10.1016/j.virol.2015.01.024
更新日期:2015-05-01 00:00:00
abstract::Sera from 215 non-drug-injecting Toba and Mataco-Mataguayo pure Indians belonging to four communities in northern Argentina were examined using assays that allow differentiation between reactivities due to type-specific antigens of the human T-cell leukemia/lymphoma virus (HTLV). Three of these populations have very l...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1631
更新日期:1993-12-01 00:00:00
abstract::The eukaryotic translation initiation factor eIF4E, the cap-binding protein, seems to play an essential role in the establishment of the host shut-off after viral infection. Infection with adenovirus and influenza virus caused dephosphorylation of eIF4E and an involvement of a viral protein was suggested. In this repo...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0143
更新日期:1996-03-15 00:00:00
abstract::Recent findings suggested that the SUMO-interacting motifs (SIMs) present in the human TRIM5α (TRIM5α(hu)) protein play an important role in the ability of TRIM5α(hu) to restrict N-MLV. Here we explored the role of SIMs in the ability of rhesus TRIM5α (TRIM5α(rh)) to restrict HIV-1, and found that TRIM5α(rh) SIM mutan...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2012.09.042
更新日期:2013-01-20 00:00:00
abstract::Gene transfer into human hematopoietic stem cells using Ad5 is inefficient due to lack of the primary receptor CAR and the secondary receptors alphavbeta3 integrin and alphavbeta5 integrin, and due to the high seroprevalence of Ad5 antibodies in most adults, resulting in diminished gene transduction. In the present st...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2004.07.018
更新日期:2004-10-25 00:00:00
abstract::The propensity of retroviruses to rapidly establish persistent infections poses a formidable problem in vaccination strategies. In the current study, we use a live attenuated vaccine to study protection against acute and persistent Friend virus infections in mice. Adoptive transfers of immune CD8(+) T cells combined w...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2000.0356
更新日期:2000-06-20 00:00:00
abstract::Marek's disease (MD) infection has been controlled effectively by vaccination using nononcogenic and/or attenuated oncogenic Marek's disease virus (MDV) vaccines. Thus far, there is little knowledge on the role of cell-mediated immune (CMI) responses during MDV infection or vaccination. To elucidate the importance of ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0400
更新日期:1996-08-01 00:00:00
abstract::Recently, the product of equine herpesvirus type 1 (EHV-1) ORF1, a homolog to HSV-1 pUL56, was shown to modulate MHC-I expression and innate immunity. Here, we investigated modulation of respiratory epithelial immunity by EHV-1 pUL56 and compared responses to those of PBMCs, which are important target cells that allow...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2014.05.023
更新日期:2014-09-01 00:00:00
abstract::The causal agent of rhizomania disease, Beet necrotic yellow vein virus (BNYVV), typically produces asymptomatic root-limited infections in sugar beets (Beta vulgaris) carrying the Rz1-allele. Unfortunately, this dominant resistance has been recently overcome. Multiple cDNA clones of the viral pathogenic determinant p...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.03.008
更新日期:2008-06-20 00:00:00
abstract::Phase I human clinical studies involving therapeutics for emerging and biodefense pathogens with low incidence, such as the severe acute respiratory syndrome coronavirus (SARS-CoV), requires at a minimum preclinical evaluation of efficacy in two well-characterized and robust animal models. Thus, a ferret SARS-CoV mode...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2007.12.032
更新日期:2008-04-25 00:00:00
abstract::Differing and sometimes conflicting data have been reported regarding several aspects of vaccinia virus (VV) entry. To address this, we used a beta-galactosidase reporter virus to monitor virus entry into multiple cell types under varying conditions. Entry into HeLa, B78H1 and L cells was strongly inhibited by heparin...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.12.029
更新日期:2009-03-15 00:00:00
abstract::The elucidation of the antigenic structure of the S glycoprotein of murine coronaviruses will provide further understanding of the complex pathogenicity of these viruses. In order to identify linear antigenic determinants, the primary structure of the S glycoprotein of murine hepatitis virus strain A59 was analyzed wi...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1376
更新日期:1994-08-01 00:00:00
abstract::We measured the effects of non-nucleoside reverse transcriptase (RT) inhibitor-resistant mutations K101E+G190S, on replication fitness and EFV-resistance of HIV(NL4-3). K101E+G190S reduced fitness in the absence of EFV and increased EFV resistance, compared to either single mutant. Unexpectedly, K101E+G190S also repli...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.03.018
更新日期:2010-07-05 00:00:00