Sequences in the 5' leader of Mason-Pfizer monkey virus which affect viral particle production and genomic RNA packaging: development of MPMV packaging cell lines.

abstract：:The yellow fever 17D virus is attenuated and used for human vaccination. Two of its substrains, 17D-204 and 17DD, are used for vaccine production. One of the remarkable properties of this vaccine is limited viral replication in the host but with significant dissemination of the viral mass, yielding a robust and long-l...

journal_title：Virology

authors： Marchevsky RS,Freire MS,Coutinho ES,Galler R

更新日期：2003-11-10 00:00:00

abstract：:The location of nucleotide sequences important in determining the extent of cowpea mosaic virus M-RNA accumulation in cowpea protoplasts has been analyzed by deletion mutagenesis of full-length cDNA clones from which infectious transcripts can be produced in vitro. The results suggest that cis-acting sequences which d...

journal_title：Virology

authors： Rohll JB,Holness CL,Lomonossoff GP,Maule AJ

更新日期：1993-04-01 00:00:00

abstract：:The RTVL-H family of human endogenous retrovirus-like sequences consists of approximately 1000 "full-length" elements and at least as many solitary RTVL-H related long terminal repeats (LTRs). We have characterized cDNA clones from two human cell libraries (Hep-2 and normal peripheral blood) and have found three clone...

journal_title：Virology

authors： Mager DL

更新日期：1989-12-01 00:00:00

abstract：:The DNA of a rat cytomegalovirus (RCMV) isolate from England is cleaved by HindIII into 25 fragments, 20 of which have been cloned into recombinant plasmids. Twenty-two of thirty KpnI fragments were similarly cloned which, together with the HindIII clones, resulted in cloning of 93% of the viral genome. Terminal fragm...

journal_title：Virology

authors： Burns WH,Barbour GM,Sandford GR

更新日期：1988-09-01 00:00:00

abstract：:A plasmid-based transfection system capable of generating infectious porcine circovirus type 2 (PCV2) was established. This system was then used in mutagenesis studies to investigate the involvement of a "conserved" nonanucleotide (which constitutes a portion of the loop sequence) located at the origin of DNA replicat...

journal_title：Virology

authors： Cheung AK

更新日期：2004-06-20 00:00:00

abstract：:Previously we have reported that the essential 3a movement gene of icosahedral cowpea chlorotic mottle virus (CCMV) can be functionally replaced by the 30-kDa movement gene of rod-shaped sunn-hemp mosaic virus (SHMV). Because plant RNA viruses differ in requiring or not requiring coat protein for systemic infection, w...

journal_title：Virology

authors： De Jong W,Mise K,Chu A,Ahlquist P

更新日期：1997-05-26 00:00:00

abstract：:Infection of lymphocytes by the Epstein-Barr virus (EBV) is initiated by attachment of the major virus glycoprotein gp350/220 to a cell surface glycoprotein, known as CR2 (CD21). In a productive infection the virus envelope fuses with host cell membranes either at the cell surface or within endocytic vesicles. To inve...

journal_title：Virology

authors： Patel RA,Hutt-Fletcher LM,Crews FT

更新日期：1993-07-01 00:00:00

abstract：:The envelope glycoprotein (Env) of Moloney murine leukemia virus (MoMuLV) is proteolytically processed and transported to the cell surface where it can be incorporated into budding virions. Cell surface Env is frequently detected using an indirect immunofluorescence assay and fluorescence-activated cell sorting (FACS)...

journal_title：Virology

authors： Yu H,Empig C,Xia J,Anderson WF

更新日期：1998-04-10 00:00:00

abstract：:In human immunodeficiency virus type 1 (HIV-1) and simian immunodeficiency virus (SIV) the gag gene encodes the precursor polyprotein Pr55Gag, which is cleaved by the viral protease to produce the major structural proteins. Recently, it has been shown that HIV and SIV gag RNAs contain internal ribosome entry sites (IR...

journal_title：Virology

authors： Nicholson MG,Barber SA,Clements JE

更新日期：2007-03-30 00:00:00

abstract：:The equine herpesvirus 1 (EHV-1) immediate-early (IE) phosphoprotein is essential for the activation of transcription from viral early and late promoters and regulates transcription from its own promoter. The IE protein of 1487 amino acids contains a serine-rich tract (SRT) between residues 181 and 220. Deletion of th...

journal_title：Virology

authors： Kim SK,Buczynski KA,Caughman GB,O'Callaghan DJ

更新日期：2001-01-05 00:00:00

abstract：:We report the discovery of Nylanderia fulva virus 1 (NfV-1), the first virus identified and characterized from the ant, Nylanderia fulva. The NfV-1 genome (GenBank accession KX024775) is 10,881 nucleotides in length, encoding one large open reading frame (ORF). Helicase, protease, RNA-dependent RNA polymerase, and jel...

journal_title：Virology

authors： Valles SM,Oi DH,Becnel JJ,Wetterer JK,LaPolla JS,Firth AE

更新日期：2016-09-01 00:00:00

abstract：:We have cloned an infectious genome of the Junonia coenia densonucleosis virus (JcDNV) into the bacterial plasmid pBR322. The viral genome could be rescued from the recombinant plasmid pBRJ by transfection of pBRJ DNA to sensitive Spodoptera littoralis larvae. pBRJ DNA produced a typical viral infection and a comparab...

journal_title：Virology

authors： Jourdan M,Jousset FX,Gervais M,Skory S,Bergoin M,Dumas B

更新日期：1990-11-01 00:00:00

abstract：:Because phosphorylation of the infectious bronchitis virus (IBV) nucleocapsid protein (N) may regulate its multiple roles in viral replication, the dynamics of N phosphorylation were examined. 32P-orthophosphate labeling and Western blot analyses confirmed that N was the only viral protein that was phosphorylated. Pul...

journal_title：Virology

authors： Jayaram J,Youn S,Collisson EW

更新日期：2005-08-15 00:00:00

abstract：:The recent identification of a novel human coronavirus responsible of a SARS-like illness in the Middle-East a decade after the SARS pandemic, demonstrates that reemergence of a SARS-like coronavirus from an animal reservoir remains a credible threat. Because SARS is contracted by aerosolized contamination of the resp...

journal_title：Virology

authors： Escriou N,Callendret B,Lorin V,Combredet C,Marianneau P,Février M,Tangy F

更新日期：2014-03-01 00:00:00

abstract：:Central African tropical forests are home to several species of non-human primates (NHPs), infected by Simian Immunodeficiency Virus (SIV). It is well-known that HIV-1 epidemic is due to cross-transmission and adaptation of SIV to humans. The main goal of this work was to investigate if a NHP bite is a risk factor for...

journal_title：Virology

authors： Filippone C,de Oliveira F,Betsem E,Schaeffer L,Fontanet A,Lemée V,Gessain A,Plantier JC

更新日期：2017-03-01 00:00:00

abstract：:The terminal sequences of both genomic dsRNA segments of different strains of infectious bursal disease virus were determined. Analysis of the linear sequences revealed in the 5'-noncoding region of both segments the presence of a 32-nucleotide sequence with motifs conserved between segments and serotypes. The 3'-nonc...

journal_title：Virology

authors： Mundt E,Müller H

更新日期：1995-05-10 00:00:00

abstract：:Chimeric primate lentiviruses composed of SIV and HIV genes may allow the analysis of the role of these discrete HIV genes in viral pathogenesis in macaque monkeys. We have constructed a chimeric virus in which the env, rev, tat, and vpu genes of HIV-1 Lai replace the env, rev, and tat genes of the SIVmac239 genome. T...

journal_title：Virology

authors： Dunn CS,Beyer C,Kieny MP,Gloeckler L,Schmitt D,Gut JP,Kirn A,Aubertin AM

更新日期：1996-09-15 00:00:00

abstract：:The epithelium of the cervix uteri has been reported to be frequently coinfected with both human papillomaviruses (HPV) and helper virus-dependent adenoassociated viruses (AAV). Seroepidemiological data suggest that AAV infection could inhibit cervical cancer that is caused by specific ("high-risk") types of papilloma...

journal_title：Virology

authors： Walz CM,Correa-Ochoa MM,Müller M,Schlehofer JR

更新日期：2002-02-01 00:00:00

abstract：:The large (L) proteins of non-segmented negative stranded (NNS) RNA viruses contain the core RNA dependent RNA polymerase activity for RNA replication and transcription as well as the activities for polyadenylating and capping the mRNA transcripts and for methylating the cap structures. There is currently no structura...

journal_title：Virology

authors： Galloway SE,Richardson PE,Wertz GW

更新日期：2008-12-05 00:00:00

abstract：:The complete nucleotide sequence of apple stem grooving virus (ASGV) genome has been determined. The genome is 6496 nucleotides in length excluding a 3'-terminal poly(A) tail and contains two overlapping open reading frames (ORFs). ORF1 begins at nucleotide position 37 and is terminated at position 6341, encoding a pr...

journal_title：Virology

authors： Yoshikawa N,Sasaki E,Kato M,Takahashi T

更新日期：1992-11-01 00:00:00

abstract：:The extraordinarily high level of genetic variation of HIV-1 env genes poses a challenge to obtain antibodies that cross-react with multiple subtype Env glycoproteins. To determine if cross-reactive monoclonal antibodies (mAbs) to highly conserved epitopes in HIV-1 envelope glycoproteins can be induced, we immunized m...

journal_title：Virology

authors： Gao F,Scearce RM,Alam SM,Hora B,Xia S,Hohm JE,Parks RJ,Ogburn DF,Tomaras GD,Park E,Lomas WE,Maino VC,Fiscus SA,Cohen MS,Moody MA,Hahn BH,Korber BT,Liao HX,Haynes BF

更新日期：2009-11-10 00:00:00

abstract：:HIV-1 genomic RNA (gRNA) dimerization is important for viral infectivity and is regulated by proteolytic processing of the Gag precursor protein (Pr55gag) under the direction of the viral protease. The processing occurs in successive steps and, to date, the step associated with formation of a wild-type (WT) level of g...

journal_title：Virology

authors： Kafaie J,Dolatshahi M,Ajamian L,Song R,Mouland AJ,Rouiller I,Laughrea M

更新日期：2009-03-01 00:00:00

abstract：:The red clover necrotic mosaic dianthovirus (RCNMV) genome is split between two single-stranded RNA species. The polycistronic RNA-1 encodes the viral RNA polymerase and capsid protein (CP) and the monocistronic RNA-2 encodes the 35 kDa cell-to-cell movement protein (MP). Nicotiana benthamiana plants transformed with ...

journal_title：Virology

authors： Vaewhongs AA,Lommel SA

更新日期：1995-10-01 00:00:00

abstract：:The prototype strain of Flanders virus (61-7484) has been adapted to grow in Vero cell cultures and a plaque assay has been developed. Electron micrographs of infected cells revealed rod-shaped virions budding in clusters from the plasma membrane, consistent with the tentative classification of Flanders virus as a mem...

journal_title：Virology

authors： Boyd KR,Whitaker-Dowling P

更新日期：1988-04-01 00:00:00

abstract：:While the C-terminal domain of HIV-1 Vpu is critical for CD4 degradation, the transmembrane domain (TM) mediates ion channel activity, enhances virus release and is essential for counteracting CD317/Bst-2/Tetherin. Here we analyzed whether the ion channel activity of Vpu is required to antagonize CD317-mediated restri...

journal_title：Virology

authors： Bolduan S,Votteler J,Lodermeyer V,Greiner T,Koppensteiner H,Schindler M,Thiel G,Schubert U

更新日期：2011-07-20 00:00:00

abstract：:Human papillomavirus type 16 E7 protein, expressed in Escherichia coli as fusion protein lac alpha peptide-E7 (lac-E7), was purified by electrophoresis and used to immunize rabbits to raise antisera. The anti-lac-E7 sera recognized another bacterially expressed fusion protein trpE-E7 by the Western blot method. The an...

journal_title：Virology

authors： Sato H,Watanabe S,Furuno A,Yoshiike K

更新日期：1989-05-01 00:00:00

abstract：:The genomic S RNA segment of Prospect Hill virus (PHV), a member of the Hantavirus genus, was molecularly cloned and the nucleotide sequence of the cDNA determined. The PHV S RNA segment is 1675 nucleotides long. A long open reading frame was identified in the viral complementary-sense RNA that could encode a 433 amin...

journal_title：Virology

authors： Parrington MA,Kang CY

更新日期：1990-03-01 00:00:00

abstract：:Many viruses utilize molecular chaperone heat shock protein 90 (Hsp90) for protein folding and stabilization, however, the role of Hsp90 in herpesvirus lifecycle is obscure. Here, we provide evidence that Hsp90 participates in pseudorabies virus (PRV) replication. Viral growth kinetics assays show that Hsp90 inhibitor...

journal_title：Virology

authors： Zhang WJ,Wang RQ,Li LT,Fu W,Chen HC,Liu ZF

更新日期：2021-01-15 00:00:00

abstract：:A full-length cDNA of cucurbit aphid-borne yellows virus (CABYV) has been constructed and expressed either as an in vitro transcript, under control of a bacteriophage T7 RNA polymerase promoter, or in vivo, under control of the cauliflower mosaic virus 35S promoter in an agroinfection vector. The biological activity o...

journal_title：Virology

authors： Prüfer D,Wipf-Scheibel C,Richards K,Guilley H,Lecoq H,Jonard G

更新日期：1995-12-01 00:00:00

abstract：:The response to influenza virus (IAV) infection and severity of disease is highly variable in humans. We hypothesized that one factor contributing to this variability is the presence of specific respiratory tract (RT) microbes. One such microbe is Streptococcus pneumoniae (Sp) that is carried asymptomatically in the R...

journal_title：Virology

authors： Wolf AI,Strauman MC,Mozdzanowska K,Williams KL,Osborne LC,Shen H,Liu Q,Garlick D,Artis D,Hensley SE,Caton AJ,Weiser JN,Erikson J

更新日期：2014-08-01 00:00:00