Abstract:
:We have cloned an infectious genome of the Junonia coenia densonucleosis virus (JcDNV) into the bacterial plasmid pBR322. The viral genome could be rescued from the recombinant plasmid pBRJ by transfection of pBRJ DNA to sensitive Spodoptera littoralis larvae. pBRJ DNA produced a typical viral infection and a comparable percentage of larvae became infected following inoculation of equivalent amounts of purified virion DNA or cloned viral DNA. Virions extracted from transfected larvae were indistinguishable from wild-type (wt) virions with regard to their biophysical and biological properties. In particular, rescued virions were as infectious as wt virions and showed identical restriction profiles of their genome. In contrast, subcloning of JcDNV DNA deleted at both extremities of a sequence of ca 250 or ca 100 bp resulted in the inability of the recombinant plasmids to initiate a viral infection. These data suggest that, as for vertebrate parvoviruses, the inverted terminal repeats display essential functions in the rescue process and replicative cycle of densoviruses. This is the first report of the molecular cloning of the infectious genome from an insect parvovirus, and more generally from an invertebrate virus. pBRJ should provide an efficient tool to further define the organization of the JcDNV genome and compare it to other parvoviruses.
journal_name
Virologyjournal_title
Virologyauthors
Jourdan M,Jousset FX,Gervais M,Skory S,Bergoin M,Dumas Bdoi
10.1016/0042-6822(90)90308-esubject
Has Abstractpub_date
1990-11-01 00:00:00pages
403-9issue
1eissn
0042-6822issn
1096-0341journal_volume
179pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::Three cDNA clones that express viral gene products in Escherichia coli JM83 were derived from a watermelon mosaic virus-1 strain of papaya ringspot virus (PRSV-W). DNAs complementary to portions of the viral RNA were inserted into the pUC8 and pUC9 plasmids, and the expressed polypeptides were fusion products with the...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90383-6
更新日期:1985-06-01 00:00:00
abstract::Characterization of the regions of human papillomaviruses (HPVs) that elicit neutralizing immune responses supports studies on viral infectivity and provides insight for the development and evaluation of prophylactic vaccines. HPV11 is a major etiologic agent of genital warts and a likely vaccine candidate. A conforma...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1999.0083
更新日期:2000-01-20 00:00:00
abstract::J-chain-deficient (Jch(-/-)) mice were used to study the role of polymeric IgA (pIgA) in primary disease and protective immunity following genital herpes simplex type 2 (HSV-2) infection. Vaginal IgA in the Jch(-/-) mice was composed primarily of monomeric IgA and was not associated with secretory component (SC). In c...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2000.0303
更新日期:2000-05-25 00:00:00
abstract::African Swine fever virus (ASFV) genome is a large (170-190 kb) double-stranded DNA molecule with structural features similar to those of Poxviruses. Prominent among those features are the presence of a hairpin loop structure at the end of the DNA molecule and terminal-inverted repeats (TIR). The TIRs have been previo...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1277
更新日期:1994-05-15 00:00:00
abstract::Infectious bronchitis virus (IBV) infection and associated illness may be dramatically modified by passive transfer of immune T lymphocytes. Lymphocytes collected 10 days postinfection were transferred to naive chicks before challenge with virus. As determined by respiratory illness and viral load, transfer of syngene...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2000.0211
更新日期:2000-03-30 00:00:00
abstract::Expression of adenosine deaminase acting on RNA1 (ADAR1) is driven by alternative promoters. Promoter PA, activated by interferon (IFN), produces transcripts that encode the inducible p150 ADAR1 protein, whereas PB specifies the constitutively expressed p110 protein. We show using Stat1(-/-), Stat2(-/-) and IRF9(-/-) ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2015.08.001
更新日期:2015-11-01 00:00:00
abstract::Nucleotide sequence determination of the coat protein cistron of the alfalfa mosaic virus (AIMV) temperature-sensitive mutant, Tbts 7 (uv) revealed a small number of point mutations of which only one results in the replacement of an amino acid: the asparagine residue at position 126 is replaced by an aspartate residue...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90609-0
更新日期:1989-08-01 00:00:00
abstract::B-HIV1, an oligoclone of immortalized cells derived from human peripheral B lymphocytes infected in vitro with the TIIIB isolate of HIV-1, produces low levels of replication-competent HIV when propagated in 1% serum, but increases production > or = 5-fold after phorbol myristate acetate (PMA) exposure. Electron micros...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1499
更新日期:1993-10-01 00:00:00
abstract::While the 2009 pandemic H1N1 virus has become established in the human population as a seasonal influenza virus, continued adaptation may alter viral virulence. Here, we passaged a 2009 pandemic H1N1 virus (A/Changchun/01/2009) in mice. Serial passage in mice generated viral variants with increased virulence. Adapted ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2015.09.014
更新日期:2015-12-01 00:00:00
abstract::Using high resolution electron microscopic autoradiography and immunocytochemistry with monoclonal antibodies against poliovirus proteins of the P2 genomic region, the location of these proteins in respect to the virus-induced vesicle formation and the viral RNA synthesis was followed during the viral replication cycl...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(87)90063-8
更新日期:1987-09-01 00:00:00
abstract::It has been suggested that, in addition to viral proteins, host proteins are involved in RNA virus replication. In this study the RNA helicase domain of the Tobacco mosaic virus (TMV) replicase proteins was used as bait in the yeast two-hybrid system to identify tobacco proteins with a putative role in TMV replication...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2002.1332
更新日期:2002-04-10 00:00:00
abstract::Alphaherpesviruses comprise closely related viruses of man and animal, including herpes simplex virus, varicella-zoster virus and pseudorabies virus (PRV). Here, using methyl-beta-cyclodextrin and fluorescently tagged PRV, we directly show that depletion of cholesterol from the plasma membrane of host cells significan...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.03.039
更新日期:2008-07-05 00:00:00
abstract::The N-terminal domains of the NS1 protein of influenza B virus (NS1B protein) and the NS1 protein of influenza A virus (NS1A protein) share one function: binding double-stranded RNA (dsRNA). Here we show that the N-terminal domain of the NS1B protein possesses an additional function that is not shared by its NS1A coun...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2002.1663
更新日期:2002-12-20 00:00:00
abstract::When hemagglutinin (HA) and neuraminidase (NA) are presented together on an intact influenza virus particle, the antigens are competitive, with HA dominant over NA in T- and B-cell priming. Immunization with mixtures of purified HA and NA eliminates antigenic competition between HA and NA, as well as between N1-N2 NA ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0581
更新日期:1996-11-01 00:00:00
abstract::We evaluated the immunogenicity and protective activity of plasmid DNA vaccines encoding the influenza virus NP gene (pNP) alone or in combination with the herpes simplex virus type 1 protein 22 gene (pVP22). Optimal immune responses were observed in BALB/c mice immunized with the combination of pVP22 plus pNP, as ass...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.04.015
更新日期:2006-10-10 00:00:00
abstract::The NIa protein of potyviruses provides VPg and proteolytic functions during virus replication. It has also been shown to confer host genotype-specific movement functions in plants. Specifically, NIa from tobacco etch virus (TEV)-Oxnard, but not from most other strains, confers the ability to move long distances in Ni...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2000.0416
更新日期:2000-08-01 00:00:00
abstract::We have compared the deduced envelope (E) protein sequences of two biologically well-characterized yellow fever (YF) virus vaccine strains. The 17DD strain has been produced in Brazil for more than 50 years and used to successfully vaccinate millions of people worldwide. The 17D-213 is a candidate vaccine strain produ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90745-b
更新日期:1992-05-01 00:00:00
abstract::The conserved nature of the epitopes of the four broadly neutralizing antibodies (BNAbs), b12, 2G12, 2F5, and 4E10, may imply that the sensitivity of HIV-1 for these BNAbs remains fairly constant over the course of infection. Here, we demonstrate that viruses isolated early during the course of infection were mostly s...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.05.028
更新日期:2009-08-01 00:00:00
abstract::RSV is a major cause of severe lower respiratory infection in infants and young children. With no vaccine yet available, it is important to clarify mechanisms of disease pathogenesis. Since indoleamine-2,3-dioxygenase (IDO) is an immunomodulatory enzyme and is upregulated with RSV infection, we studied it in vivo duri...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2017.09.007
更新日期:2017-12-01 00:00:00
abstract::Adenoviruses (Ads) infect hosts from all vertebrate species and have been investigated as vaccine vectors. We report here near-atomic structures of two bovine Ad type 3 (BAd3) intermediates obtained by cryo-electron microscopy. A comparison between the two intermediate structures reveals that the differences are local...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2013.12.012
更新日期:2014-02-01 00:00:00
abstract::Previous studies have shown that a 558-bp fragment of human cytomegalovirus (CMV) DNA contained within pCM4127 and designated CMV mtr can morphologically transform rodents cells in vitro. By cotransformation with pCM4127 and a plasmid conferring G418 resistance, pSV2neo, morphologically transformed NIH3T3 cell lines w...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(87)90437-5
更新日期:1987-01-01 00:00:00
abstract::A series of clones has been derived from an interferon-resistant murine cell line, Ltk- aprt-, and their antiviral properties have been characterized. In the parental Ltk- aprt- line interferon is unable to establish antiviral properties or to increase the levels of 2,5-oligo(A) synthetase, the 2,5-oligo(A)-activated ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90400-x
更新日期:1988-01-01 00:00:00
abstract::A nonaphid transmissible (NAT) variant of tobacco vein mottling virus (TVMV) was used to test the hypothesis that the viral coat protein (CP) plays a role in determining aphid transmissibility. Comparison of the nucleotide sequences in the coat protein cistron of an aphid transmissible isolate (TVMV-AT) with that of T...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90389-9
更新日期:1990-09-01 00:00:00
abstract::Highly pathogenic alphaviruses display complex glycans on their surface. These glycans play a crucial role in viral pathogenesis by facilitating glycan-host interaction during viral entry which can be targeted. Various studies have reported antiviral activity of lectins that bind to the glycans present on the surface ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2018.10.009
更新日期:2019-01-02 00:00:00
abstract::Virus-host interactions essential for alphavirus pathogenesis are poorly understood. To address this shortcoming, we coupled retrovirus insertional mutagenesis and a cell survival selection strategy to generate clonal cell lines broadly resistant to Sindbis virus (SINV) and other alphaviruses. Resistant cells had sign...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.03.025
更新日期:2008-07-20 00:00:00
abstract::Neutralizing sites on bluetongue virus serotype 10 (BTV-10) were investigated with a panel of seven neutralizing monoclonal antibodies (MAbs). Each MAb was coupled to agarose beads and tested against the other MAbs and a nonneutralizing control MAb in a competitive immueprecipitation assay. In addition, neutralization...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90041-o
更新日期:1990-06-01 00:00:00
abstract::Major efforts have been put forth for the development of effective rotavirus vaccines including transgenic plant vaccines. Previous studies have reported that rotavirus VP7 maintains its neutralizing immunity when it is transformed into the potato genome. The present study was aimed at investigating the hereditary sta...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.07.045
更新日期:2006-12-05 00:00:00
abstract::Attachment of human adenoviruses (Ads) to host cells is mediated by the interaction of the fiber protein of the capsid with specific cell-surface molecules. For one of the species B adenoviruses, Ad3, the mechanism of binding to cells remains to be defined. Several previous reports have proposed CD46, CD80 or CD86 as ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.07.010
更新日期:2009-09-30 00:00:00
abstract::Human herpesvirus 6 (HHV-6) is a ubiquitous betaherpesvirus that predominantly infects and replicates in CD4(+) T lymphocytes. However, the mechanism of HHV-6 transmission to T cells from the peripheral mucosa is unknown. Here we found that dendritic cells (DCs) can transmit HHV-6 to T cells, resulting in productive i...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.11.049
更新日期:2009-03-15 00:00:00
abstract::This study evaluates the role of antibody as an indicator of immunity to ocular challenge with herpes simplex virus (HSV). Two genotypes of mice, BALB/c or BALB/c with mu-chain knockout (muK/O; which lack functional B cells), were immunized systemically either with nonvirulent infectious virus or with a eukaryotic exp...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1999.9613
更新日期:1999-04-25 00:00:00