Abstract:
:Major efforts have been put forth for the development of effective rotavirus vaccines including transgenic plant vaccines. Previous studies have reported that rotavirus VP7 maintains its neutralizing immunity when it is transformed into the potato genome. The present study was aimed at investigating the hereditary stability of VP7-transformed potatoes over fifty generations. The VP7 gene was stably transcribed and expressed in potato cells as detected by RT-PCR and Western blotting. Humeral and mucosal responses were successfully induced in BALB/c mice fed with the fiftieth generation transformed potato tubers. There were no significant differences in serum IgG and fecal IgA between the mice fed with the first and fiftieth generation potatoes (P>0.05). Profiles of cytokines such as IFN-gamma, IL-2, IL-4, IL-5 and TGF-beta in immunized mice showed a naive T-cells bias to Th1 and Th3 polarization. Moreover, specific CTL responses were also detected in C57BL/6 mice fed with transformed potatoes. This research represents a significant step towards the development of rotavirus vaccines derived from a transgenic plant that can be obtained by long-term and large-scale vegetative reproduction. To our knowledge, this is the first finding regarding vaccines derived from plants that can be propagated for many generations.
journal_name
Virologyjournal_title
Virologyauthors
Li JT,Fei L,Mou ZR,Wei J,Tang Y,He HY,Wang L,Wu YZdoi
10.1016/j.virol.2006.07.045subject
Has Abstractpub_date
2006-12-05 00:00:00pages
171-8issue
1-2eissn
0042-6822issn
1096-0341pii
S0042-6822(06)00524-1journal_volume
356pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::To identify cellular factors that interact with hepatitis C virus RNA, cellular extracts were subjected to UV cross-linking to radiolabeled RNAs corresponding to the hepatitis C virus 5' and 3' untranslated regions of positive and negative polarities. Our results demonstrate that the U-rich region of the hepatitis C v...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2000.0461
更新日期:2000-09-01 00:00:00
abstract::Hantaan virus, the prototype virus of hemorrhagic fever with renal syndrome, was examined for nucleic acid characteristics which would support its previously proposed inclusion in the virus family Bunyaviridae. Nucleocapsid RNA from Hantaan virions and a control bunyavirus were examined for ribonuclease A (RNase A) se...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(83)90514-7
更新日期:1983-12-01 00:00:00
abstract::Serial passage of Ross River virus in chick embryo fibroblasts selected for virus variants altered in their reactions to neutralizing monoclonal antibodies. The E1 and E2 genes of each antigenic variant were sequenced; single nucleotide changes were found in E2 leading to amino acid substitutions at either residue 4 (...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1143
更新日期:1993-03-01 00:00:00
abstract::A map of cleavage sites for the restriction endonucleases EcoRI, HindIII, BamHI, HpaI, and KpnI for a New Zealand strain of orf virus (NZ2) DNA has been deduced. Also, the entire genome, apart from approximately 0.1 kbp at each end, has been cloned into various vectors. The genome is 139 kbp in length and, in common w...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(87)90307-2
更新日期:1987-03-01 00:00:00
abstract::The retroviral cyclin protein (rv-cyclin) of walleye dermal sarcoma virus contains two known functional domains, a cyclin box motif and a carboxy terminal transcription activation domain (AD). The AD contacts TATA-binding protein-associated factor 9 (TAF9), and this action is necessary for both positive and negative r...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.12.026
更新日期:2009-03-30 00:00:00
abstract::To celebrate the sixtieth anniversary of Virology a survey is made of the plant viruses, virologists and their institutions, and tools and technology described in the first decade of plant virus publications in Virology. This was a period when plant viruses increasingly became tools of discovery as epistemic objects a...
journal_title:Virology
pub_type: 历史文章,杂志文章,评审
doi:10.1016/j.virol.2015.03.019
更新日期:2015-05-01 00:00:00
abstract::In 2014-2015, the U.S. experienced an unprecedented outbreak of Eurasian clade 2.3.4.4 H5 highly pathogenic avian influenza (HPAI) virus, initially affecting mainly wild birds and few backyard and commercial poultry premises. To better model the outbreak, the pathogenesis and transmission dynamics of representative Eu...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2016.04.019
更新日期:2016-07-01 00:00:00
abstract::Circoviruses are relatively novel pathogens with increased importance in canids. In this study, we first screened the presence of dog circovirus (DogCV) by molecular methods from a total number of 389 internal organ samples originating from 277 individuals of domestic dogs and wild animals including wolves, foxes and ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2016.01.007
更新日期:2016-03-01 00:00:00
abstract::The circular single-stranded viral DNA (ssDNA) of filamentous phage is oriented in the virus with a hairpin forming sequence called mos at the leading end of the virion-the end that emerges from the cell first. In the experiments that originally defined mos it was also found to enhance virion production 100-fold, thou...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90066-9
更新日期:1988-11-01 00:00:00
abstract::The adenine DNA methyltransferase M.CviRI (TGCmA) gene from chlorella virus XZ-6E was cloned into the Autographa californica nuclear polyhedrosis virus (AcMNPV) genome and expressed in Spodoptera frugiperda insect cells under the control of two tandemly arranged viral promoters, the early ETL promoter and the late pol...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1539
更新日期:1993-10-01 00:00:00
abstract::The induction of autoimmunity by viruses has been hypothesized to occur by a number of mechanisms. Coxsackievirus B4 (CB4) induces hyperglycemia in SJL mice resembling diabetes in humans. While virus is effectively cleared within 2 weeks, hyperglycemia does not appear until about 8-12 weeks postinfection at a time whe...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(03)00462-8
更新日期:2003-09-30 00:00:00
abstract::An SH3-binding domain within the Nef protein of primate lentiviruses has been reported to be important to viral replication and infectivity and dispensable for CD4 downregulation, but its precise role remains unclear. This study investigates the effects of mutations in both the polyproline helix and in the hydrophobic...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1999.9897
更新日期:1999-09-15 00:00:00
abstract::We recently developed an in vitro transcapsidation system in which infectivity of single-shelled (ss) rotavirus particles was successfully rescued (Chen and Ramig, Virology [1993]). Here, we report the rescue of infectivity of rotavirus core particles using virus strain B223 (G serotype 10) as the core donor and strai...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1315
更新日期:1993-06-01 00:00:00
abstract::The pathogenesis of two Californian strains of myxoma virus (MSW and MSD) was examined in European rabbits (Oryctolagus cuniculus) that were either susceptible to myxomatosis (laboratory rabbits) or had undergone natural selection for genetic resistance to myxomatosis (Australian wild rabbits). MSW was highly lethal f...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.12.007
更新日期:2006-04-25 00:00:00
abstract::HIV-1 virus entry into target cells requires the envelope glycoprotein (Env) to first bind the primary receptor, CD4 and subsequently the co-receptor. Antibody access to the co-receptor binding site (CoRbs) in the pre-receptor-engaged state, prior to cell attachment, remains poorly understood. Here, we have demonstrat...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2018.04.002
更新日期:2018-06-01 00:00:00
abstract::Following CD4 receptor binding to the HIV-1 envelope spike (Env), the conserved N-heptad repeat (NHR) region of gp41 forms a coiled-coil that is a precursor to the fusion reaction. Although it has been a target of drug and vaccine design, there are few monoclonal antibody (mAb) tools with which to probe the antigenici...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.04.005
更新日期:2008-07-20 00:00:00
abstract::The molecular host response to hepatitis C virus (HCV) infection was examined by isolation of HCV-induced genes from a cDNA library constructed from chimpanzee liver during the acute phase of hepatitis C. Two cDNA clones, 130-7 and 130-51, were obtained by differential hybridization with cDNA probes prepared from poly...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90925-f
更新日期:1992-10-01 00:00:00
abstract::Soluble RNA-dependent RNA polymerases from healthy and alfalfa mosaic virus (AMV)-infected leaves were purified more than 400-fold from the 100,000g supernatant of leaf homogenates, using ammonium sulfate precipitation, Sephadex G-100 filtration, and chromatography on DEAE Sephadex A25 and P11-phosphocellulose. The DE...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(80)90554-1
更新日期:1980-01-15 00:00:00
abstract::HIV-1 variants resistant to small molecule CCR5 inhibitors such as vicriviroc (VVC) have modified Env complexes that can use both the inhibitor-bound and -free forms of the CCR5 co-receptor to enter target cells. However, entry via the inhibitor-CCR5 complex is inefficient in some, but not all, cell types, particularl...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.02.044
更新日期:2009-05-10 00:00:00
abstract::Many viruses utilize molecular chaperone heat shock protein 90 (Hsp90) for protein folding and stabilization, however, the role of Hsp90 in herpesvirus lifecycle is obscure. Here, we provide evidence that Hsp90 participates in pseudorabies virus (PRV) replication. Viral growth kinetics assays show that Hsp90 inhibitor...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2020.10.013
更新日期:2021-01-15 00:00:00
abstract::At least four hantavirus species cause disease with prominent renal involvement-hemorrhagic fever with renal syndrome (HFRS); and several hantavirus strains cause disease with significant pulmonary involvement-hantavirus pulmonary syndrome (HPS). The most prevalent and lethal hantaviruses associated with HFRS and HPS ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.11.035
更新日期:2006-03-30 00:00:00
abstract::Unlike cell lines transformed with Polyomaviruses, transformants derived by focus formation or colony formation in agarose medium after transfer into rat fibroblast cells (FR3T3 line) of Bovine Papilloma Type 1 (BPV1) DNA were consistently observed to grow poorly in suspension and to remain highly serum dependent for ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90196-x
更新日期:1984-06-01 00:00:00
abstract::Antisera with Fiji disease virus (FDV) protein-specific and double-stranded (ds) RNA-specific antibodies were prepared by injecting rabbits with highly purified preparations of FDV cores. Electron microscopic examination of negatively stained core preparations disclosed the presence of icosahedral particles, about 54 ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(79)90495-1
更新日期:1979-06-01 00:00:00
abstract::Here we describe the complete genome sequences of two strains of Usutu virus (USUV), a mosquito-borne member of the genus Flavivirus in the Japanese encephalitis virus (JEV) serogroup. USUV was detected in Austria in 2001 causing a high mortality rate in blackbirds; the reference strain (SAAR-1776) was isolated in 195...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2004.08.005
更新日期:2004-10-25 00:00:00
abstract::Despite significant contributions of monocytes to HIV persistence, the genomic basis of HIV-infection of monocytes and its association with plasma viremia remain elusive. To understand HIV interactions with monocytes during disease progression, monocytic transcriptomes from long-term non-progressors (LTNP), HIV+ patie...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2012.10.026
更新日期:2013-01-20 00:00:00
abstract::Genetic exchanges constitute a significant means by which bacteriophages acquire novel characteristics. Phages of Lactococcus lactis occupy a particular niche, the dairy factory environment, where their populations are subjected to constant changes. Little is known about the mechanisms of evolution that lead to the ge...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2000.0226
更新日期:2000-04-25 00:00:00
abstract::African Swine fever virus (ASFV) genome is a large (170-190 kb) double-stranded DNA molecule with structural features similar to those of Poxviruses. Prominent among those features are the presence of a hairpin loop structure at the end of the DNA molecule and terminal-inverted repeats (TIR). The TIRs have been previo...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1277
更新日期:1994-05-15 00:00:00
abstract::We have constructed a recombinant baculovirus expressing high levels of the herpes simplex virus type 1 (HSV-1) glycoprotein E (gE) in Sf9 cells. The expressed gE migrated on gels as a double band with apparent molecular weights of 68 and 70 kDa. The recombinant gE was glycosylated based on its susceptibility to tunic...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90500-o
更新日期:1992-06-01 00:00:00
abstract::In retrovirus-induced tumors, proviral DNA is commonly found next to or within a cellular proto-oncogene such that transcription of the gene is influenced by the viral promoter or enhancer. Extensive surveys of naturally occurring tumors reveal that proviruses integrated on the 3' side of the gene are usually in the s...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1245
更新日期:1993-05-01 00:00:00
abstract::The nucleoprotein (NP) of the influenza A virus encapsidates the viral RNA and participates in the infectious life cycle of the virus. The aims of this study were to find the degree of conservation of NP among all virus subtypes and hosts and to identify conserved binding sites, which may be utilised as potential drug...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2014.01.023
更新日期:2014-04-01 00:00:00