Abstract:
:HIV-1 virus entry into target cells requires the envelope glycoprotein (Env) to first bind the primary receptor, CD4 and subsequently the co-receptor. Antibody access to the co-receptor binding site (CoRbs) in the pre-receptor-engaged state, prior to cell attachment, remains poorly understood. Here, we have demonstrated that for tier-1 Envs, the CoRbs is directly accessible to full-length CD4-induced (CD4i) antibodies even before primary receptor engagement, indicating that on these Envs the CoRbs site is either preformed or can conformationally sample post-CD4-bound state. Tier-2 and tier-3 Envs, which are resistant to full-length CD4i antibody, are neutralized by m36.4, a lower molecular mass of CD4i-directed domain antibody. In some tier-2 and tier-3 Envs, CoRbs is accessible to m36.4 even prior to cellular attachment in an Env-specific manner independent of their tier category. These data suggest differential structural arrangements of CoRbs and varied masking of ligand access to the CoRbs in different Env isolates.
journal_name
Virologyjournal_title
Virologyauthors
Boliar S,Patil S,Shukla BN,Ghobbeh A,Deshpande S,Chen W,Guenaga J,Dimitrov DS,Wyatt RT,Chakrabarti BKdoi
10.1016/j.virol.2018.04.002subject
Has Abstractpub_date
2018-06-01 00:00:00pages
99-105eissn
0042-6822issn
1096-0341pii
S0042-6822(18)30110-7journal_volume
519pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::The complete nucleotide sequence of the middle component RNA (M RNA) of the comovirus bean pod mottle virus (BPMV) has been determined. The sequence consists of 3662 nucleotides and contains a single long open reading frame sufficient to code for a protein of 113,353 Da. The proteolytic processing sites within this pr...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90155-5
更新日期:1991-07-01 00:00:00
abstract::Economic loss due to viral endothelial cell necrosis of eel (VECNE) of Anguilla japonica is a serious problem for the cultured Japanese eel market. However, the viral genome responsible for VECNE is unknown. We recently developed a rapid determination system for viral nucleic acid sequences (RDV) to determine viral ge...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.12.057
更新日期:2011-03-30 00:00:00
abstract::The nucleotide and deduced amino acid sequence of the gene 4 of murine rotavirus strain Eb were determined. The gene is 2359 nucleotides in length and encodes for a protein of 775 amino acids. Comparison of the VP4 amino acid sequence of the Eb strain with several human and animal rotavirus strains which represent all...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1153
更新日期:1994-03-01 00:00:00
abstract::The promotion of membrane fusion by the paramyxovirus hemagglutinin-neuraminidase (HN) and fusion (F) proteins requires that they be derived from homologous viruses, suggesting the possibility that the promotion of fusion requires a virus-specific communication between the two glycoprotein spikes. We have evaluated th...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.1278
更新日期:1995-06-01 00:00:00
abstract::To investigate the mode of gene expression of red clover mottle virus (RCMV) middle component (M) RNA, we have synthesized an oligopeptide corresponding to the predicted carboxy-terminus of the RCMV counterparts of the cowpea mosaic virus (CPMV) 48K and 58K proteins. Using an antiserum raised against this synthetic ol...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90552-7
更新日期:1989-12-01 00:00:00
abstract::HIV-1 infection leads to impaired antigen-specific T cell proliferation, increased susceptibility of T cells to apoptosis, progressive impairment of T-helper 1 (Th1) responses, and altered maturation of HIV-1-specific memory cells. We have identified similar impairments in HIV-1 transgenic (Tg) rats. Tg rats developed...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2003.12.010
更新日期:2004-03-30 00:00:00
abstract::The amino acid sequence of the coat protein of wild-type cowpea chlorotic mottle virus has been nearly completely determined by direct methods. The sites of amino acid replacements were identified in the coat proteins of an oxidation-sensitive mutant, a temperature-sensitive mutant, and a salt-stable mutant. The repla...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(82)90108-8
更新日期:1982-06-01 00:00:00
abstract::Transcription of the paramyxovirus RNA genome is thought to involve a sequential stop-start mechanism whereby monocistronic mRNAs are produced by polyadenylation and termination of 3' upstream gene followed by reinitiation at the downstream start site. For a number of paramyxoviruses, transcription across the M-F gene...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9266
更新日期:1998-08-01 00:00:00
abstract::To determine the direction of the entry of DNA during in vitro bacteriophage T7 DNA packaging, incompletely packaged DNA (ipDNA) was fractionated by agarose gel electrophoresis after degradation of DNA outside of capsids and then release of packaged DNA from capsids. After fractionation, quantitative in-gel probing wi...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1476
更新日期:1993-09-01 00:00:00
abstract::An ideal system to investigate individual determinants of the replication process of (+)-strand RNA viruses is a cell-free extract that supports viral protein and RNA synthesis in a synchronized manner. Here, we applied a translation/replication system based on cytoplasmic extracts of Nicotiana tabacum cells to Tomato...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.05.009
更新日期:2009-08-01 00:00:00
abstract::While DNA vaccines have been shown to prime cellular immune responses, levels are often low in nonhuman primates or humans. Hence, efforts have been directed toward boosting responses by combining DNA with different vaccination modalities. To this end, a polyvalent DNA prime/protein boost vaccine, consisting of codon ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.10.038
更新日期:2006-03-01 00:00:00
abstract::Here we completed the whole genome sequence of Cotesia vestalis bracovirus (CvBV) by deep sequencing and compared the genome features of CvBV to those of other polydnaviruses (PDVs). The genome is 540,215 base pairs divided into 35 genomic segments that range from 2.6 to 39.2kb. Comparison of CvBV with other PDVs show...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2011.03.009
更新日期:2011-05-25 00:00:00
abstract::We have shown that polyamide nucleic acids (PNAs) targeted to the PBS (PNA(PBS)) and A-loop (PNA(A-loop)) sequences, when transfected into cells, inhibit HIV-1 replication by blocking the initiation of reverse transcription via destabilizing tRNA(3)(Lys) primer from the viral genome. Here we demonstrate that both PNA(...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2007.01.016
更新日期:2007-06-20 00:00:00
abstract::Human immunodeficiency virus type 1 subtype C (HIV-1C) accounts for about 50% of all HIV infections in the pandemic and is the predominant subtype in the heavily burdened region of southern Africa. HIV-1C possesses unique genetic and phenotypic features that might be associated with biological differences compared to ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.11.047
更新日期:2006-04-10 00:00:00
abstract::The monoclonal antibody 2D5 neutralized vaccinia virus by preventing penetration of the virus and reacting with VP23-29K. The conformation of the VP23-29K was maintained by a disulfide bond(s), and the 2D5mAb reacted stronger with the nonreduced 23-kDa form than with the reduced 29-kDa form. We selected several escape...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0337
更新日期:1996-06-15 00:00:00
abstract::The VP6 gene of human group C rotavirus was cloned and sequenced. Hybridization to the human group C and the porcine group C/Cowden dsRNA genomes assigned this coding sequence to segment 5. The complete human VP6 sequence contained an open reading frame of 1185 nucleotides (395 amino acids; deduced Mr 44,669 Da). The ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90452-h
更新日期:1991-10-01 00:00:00
abstract::Tombusviruses, of which tomato bushy stunt virus (TBSV) is the type member, spontaneously generate defective interfering RNAs (DIs) that are known to interfere with viral accumulation and symptom development. We show that co-infection with TBSV and DIs causes a dramatic reduction in accumulation of TBSV subgenomic RNA...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.1410
更新日期:1995-08-01 00:00:00
abstract::Although v-src, the oncogene of Rous sarcoma virus, has been shown to be capable of inducing lethal tumors at visceral sites distal to the primary tumor mass, the mechanisms opposing visceral tumor formation remain to be elucidated. In the present study, we show that visceral tumors, many of which represent a metastas...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1617
更新日期:1993-11-01 00:00:00
abstract::We have previously reported that a novel herpes simplex virus RNA transcript partially overlapping the gene encoding ICPO and expressed from the opposite DNA strand is abundant in sensory neurons of mice harboring a latent infection [J.G. Stevens, E.K. Wagner, G.B. Devi-Rao, M.L. Cook, and L.T. Feldman, Science 235, 1...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90169-9
更新日期:1988-09-01 00:00:00
abstract::The cowpea strain of Southern bean mosaic virus (SBMV-C), a T = 3 icosahedral RNA virus, was dissociated to yield a ribonucleoprotein complex (RNPC) composed of the viral RNA and coat protein subunits. To determine if the coat protein subunits were bound to a specific site on the viral RNA, the RNPC was treated with r...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.1117
更新日期:1995-03-10 00:00:00
abstract::Human noroviruses bind histo-blood group antigens (HBGAs) and this interaction is thought to be important for an infection. We identified two additional fucose-binding pockets (termed fucose-3/4 sites) on a genogroup II human (GII.10) norovirus-protruding (P) dimer using X-ray crystallography. Fucose-3/4 sites were lo...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2015.04.006
更新日期:2015-09-01 00:00:00
abstract::We examined nine dengue virus-specific human CD4+ CD8- cytotoxic T lymphocyte (CTL) clones for protein recognition, using recombinant vaccinia viruses which contain genes coding for dengue virus proteins. These clones were established from peripheral blood mononuclear cells of a donor previously immunized with a live-...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8648
更新日期:1997-08-04 00:00:00
abstract::The splice acceptor site of pX gene of HTLV-I has been determined to be at base position 7301 using S1 nuclease protection analysis. This splice acceptor site is used in all HTLV-I immortalized T-cell clones studied despite variation in the abundance levels of pX mRNA. Our results confirmed the proposal by Haseltine e...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90404-0
更新日期:1985-06-01 00:00:00
abstract::Marek's disease virus (MDV) is an oncogenic alphaherpesvirus of Gallus gallus, the domesticated chicken. Control strategies rely upon vaccination with live attenuated viruses of antigenically similar avian herpesviruses or attenuated strains of MDV. Recent studies in other viruses have shown that recoding certain vira...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2018.01.020
更新日期:2018-03-01 00:00:00
abstract::The sequence of Broadhaven (BRD) virus segment 5, the major genetic determinant of serotype, is 1658 nucleotides in length and contains a single open reading frame (ORF) having the coding capacity for a protein of Mr 52.5K. Comparison of the ORF of segment 5 of BRD virus with published sequences of bluetongue virus (B...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90320-q
更新日期:1990-11-01 00:00:00
abstract::Adeno-associated virus (AAV-2) replicates to high titers when host cells are coinfected with a helper virus. Here we analyzed the coinfection of AAV-2 and mouse adenovirus (MAV-1) in murine fibroblasts. We observed that AAV-2/MAV-1 coinfected NIH 3T3 cells produced approximately 10-40-fold less AAV-2 DNAse resistant p...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.04.020
更新日期:2009-07-20 00:00:00
abstract::We have proposed that chronic infection of keratinocytes by HPV modifies the expression of potentially important cytokines by interfering with the NF-kappaB signal pathway. We evaluated the constitutive and IL-1beta-induced expression of GM-CSF and TNF-alpha and the expression/activity of NF-kappaB in HPV+ and HPV- ce...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2002.1468
更新日期:2002-07-05 00:00:00
abstract::The 2009 influenza pandemic originated from a swine-origin H1N1 virus, which, although less pathogenic than anticipated, may acquire additional virulence-associated mutations in the future. To estimate the potential risk, we sequentially passaged the isolate A/Hamburg/04/2009 in A549 human lung epithelial cells. After...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2016.02.002
更新日期:2016-05-01 00:00:00
abstract::We previously showed that reduced infectivity of HIV with incompletely processed capsid-spacer protein 1 (CA-SP1) is rescued by cellular activation or increased expression of HSP90AB1, a member of the cytosolic heat shock protein 90 family. Here we show that HSP90AB1 is present in HIV virions and that HSP90AB1, but no...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2012.11.005
更新日期:2013-02-05 00:00:00
abstract::It is now widely recognized that BPV-1 and less commonly BPV-2 are the causative agents of equine sarcoids. Here we present the generation of equine cell lines harboring BPV-1 genomes and expressing viral genes. These lines have been either explanted from sarcoid biopsies or generated in vitro by transfection of prima...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2007.11.037
更新日期:2008-04-10 00:00:00