Molecular cloning, sequence analysis and coding assignment of the major inner capsid protein gene of human group C rotavirus.


:The VP6 gene of human group C rotavirus was cloned and sequenced. Hybridization to the human group C and the porcine group C/Cowden dsRNA genomes assigned this coding sequence to segment 5. The complete human VP6 sequence contained an open reading frame of 1185 nucleotides (395 amino acids; deduced Mr 44,669 Da). The protein sequence demonstrated low homology with the group A VP6 sequences (41.7 to 42.7%) and high homology (88.9%) with the porcine group C VP6 sequence. However, the protein sequence alignments revealed a region of 10 amino acids that were significantly different between the human and the porcine group C viruses.






Cooke SJ,Lambden PR,Caul EO,Clarke IN




Has Abstract


1991-10-01 00:00:00














  • Quasispecies variation of porcine reproductive and respiratory syndrome virus during natural infection.

    abstract::Porcine reproductive and respiratory syndrome virus (PRRSV) displays notorious genetic, antigenic, and clinical variability. Little is known, however, about the nature and extent of viral variation present within naturally infected animals. By amplifying and cloning the open reading frame 5 gene from tonsils of natura...


    pub_type: 杂志文章


    authors: Goldberg TL,Lowe JF,Milburn SM,Firkins LD

    更新日期:2003-12-20 00:00:00

  • Influence of CCR7 ligand DNA preexposure on the magnitude and duration of immunity.

    abstract::The CC chemokine receptor (CCR) 7 ligands CCL21 and CCL19 were recently described as essential elements for establishing the microenvironment needed to initiate optimal immune responses in secondary lymphoid tissues. In the present study we have kinetically investigated the primary responses of naive DO11.10 TCR-trans...


    pub_type: 杂志文章


    authors: Lee Y,Eo SK,Rouse RJ,Rouse BT

    更新日期:2003-07-20 00:00:00

  • Association of toll-like receptor polymorphisms with susceptibility to chikungunya virus infection.

    abstract::Chikungunya virus (CHIKV) infection leads to activation of innate immune response by triggering Toll-like receptor (TLR) pathways resulting in elevated cytokines and type-I interferon levels. Genetic variations of these genes may influence human CHIKV-susceptibility and disease progression. Present study aimed to iden...


    pub_type: 杂志文章


    authors: Dutta SK,Tripathi A

    更新日期:2017-11-01 00:00:00

  • Construction, characterization, and utilization of cell lines which inducibly express the adenovirus DNA-binding protein.

    abstract::To further our understanding of structure-function relationships within the multifunctional adenovirus DNA binding protein (DBP) a more diverse collection of mutants is necessary. DBP-expressing cell lines (gmDBP) were previously constructed that complemented DBP-negative mutants for viral growth. However, they did no...


    pub_type: 杂志文章


    authors: Brough DE,Cleghon V,Klessig DF

    更新日期:1992-10-01 00:00:00

  • Attenuating mutations in the E2 glycoprotein gene of Venezuelan equine encephalitis virus: construction of single and multiple mutants in a full-length cDNA clone.

    abstract::Attenuated mutants of Venezuelan equine encephalitis virus (VEE) were isolated by selection for rapid penetration of cultured cells (R. E. Johnston and J. F. Smith, 1988, Virology 162, 437-443). Sequence analysis of these mutants identified candidate attenuating mutations at four loci in the VEE E2 glycoprotein gene: ...


    pub_type: 杂志文章


    authors: Davis NL,Powell N,Greenwald GF,Willis LV,Johnson BJ,Smith JF,Johnston RE

    更新日期:1991-07-01 00:00:00

  • Recombinant human parainfluenza virus type 2 with mutations in V that permit cellular interferon signaling are not attenuated in non-human primates.

    abstract::The HPIV2 V protein inhibits type I interferon (IFN) induction and signaling. To manipulate the V protein, whose coding sequence overlaps that of the polymerase-associated phosphoprotein (P), without altering the P protein, we generated an HPIV2 virus in which P and V are expressed from separate genes (rHPIV2-P+V). rH...


    pub_type: 杂志文章


    authors: Schaap-Nutt A,D'Angelo C,Amaro-Carambot E,Nolan SM,Davis S,Wise SM,Higgins C,Bradley K,Kim O,Mayor R,Skiadopoulos MH,Collins PL,Murphy BR,Schmidt AC

    更新日期:2010-10-10 00:00:00

  • Injection of the sciatic nerve with TMEV: a new model for peripheral nerve demyelination.

    abstract::Demyelination of the human peripheral nervous system (PNS) can be caused by diverse mechanisms including viral infection. Despite association of several viruses with the development of peripheral demyelination, animal models of the condition have been limited to disease that is either autoimmune or genetic in origin. ...


    pub_type: 杂志文章


    authors: Drescher KM,Tracy SM

    更新日期:2007-03-01 00:00:00

  • The Potyviridae P1a leader protease contributes to host range specificity.

    abstract::The P1a protein of the ipomovirus Cucumber vein yellowing virus is one of the self-cleavage serine proteases present in Potyviridae family members. P1a is located at the N-terminal end of the viral polyprotein, and is closely related to potyviral P1 protease. For its proteolytic activity, P1a requires a still unknown ...


    pub_type: 杂志文章


    authors: Shan H,Pasin F,Valli A,Castillo C,Rajulu C,Carbonell A,Simón-Mateo C,García JA,Rodamilans B

    更新日期:2015-02-01 00:00:00

  • Analysis of the phosphorylation status of Epstein-Barr virus LMP2A in epithelial cells.

    abstract::LMP2A deletion and point mutants, with mutations in phosphotyrosine-containing protein-protein interaction motifs, were transiently expressed in 293 cells and their phosphorylation was examined in immune complex kinase assays as well as in vivo. In vitro LMP2A phosphorylation depended on tyrosine 112. In vivo, mutatio...


    pub_type: 杂志文章


    authors: Scholle F,Longnecker R,Raab-Traub N

    更新日期:2001-12-20 00:00:00

  • Receptor determinants of human and animal influenza virus isolates: differences in receptor specificity of the H3 hemagglutinin based on species of origin.

    abstract::The binding of influenza virus to erythrocytes and host cells is mediated by the interaction of the viral hemagglutinin (H) with cell surface receptors containing sialic acid (SA). The specificity of this interaction for 19 human and animal influenza isolates was examined using human erythrocytes enzymatically modifie...


    pub_type: 杂志文章


    authors: Rogers GN,Paulson JC

    更新日期:1983-06-01 00:00:00

  • The p.Ser267Phe variant of sodium taurocholate cotransporting polypeptide (NTCP) supports HBV infection with a low efficiency.

    abstract::Sodium taurocholate cotransporting polypeptide (NTCP) is a functional receptor for human hepatitis B virus (HBV) and its satellite virus Hepatitis D virus (HDV). Physiologically, NTCP is responsible for the majority of sodium-dependent bile acids uptake by hepatocytes. The p.Ser267Phe (S267F) variant of NTCP is a sing...


    pub_type: 杂志文章


    authors: Liu C,Xu G,Gao Z,Zhou Z,Guo G,Li D,Jing Z,Sui J,Li W

    更新日期:2018-09-01 00:00:00

  • Genetic variation among dengue 2 viruses of different geographic origin.

    abstract::Genetic variation in dengue 2 isolates from various geographic areas was examined by oligonucleotide fingerprinting of the 40 S genome RNA. Oligonucleotide maps of geographically isolated and epidemiologically unrelated viruses were very distinct. Direct comparison of the oligonucleotide map of the dengue 2 prototype ...


    pub_type: 杂志文章


    authors: Trent DW,Grant JA,Rosen L,Monath TP

    更新日期:1983-07-30 00:00:00

  • Homotypic interaction and multimerization of hepatitis C virus core protein.

    abstract::Hepatitis C virus (HCV) core protein constitutes a viral nucleocapsid and may possess multiple functions. In this study, we demonstrated the homotypic interaction and multimerization of HCV core protein in vitro and in vivo. By using a yeast two-hybrid system, we showed that the amino-terminal hydrophilic portion (ami...


    pub_type: 杂志文章


    authors: Matsumoto M,Hwang SB,Jeng KS,Zhu N,Lai MM

    更新日期:1996-04-01 00:00:00

  • Differential growth of human enteric adenovirus 41 (TAK) in continuous cell lines.

    abstract::Differing reports exist about the replication of human enteric adenoviruses (EnAds) in various cell lines. There was a suggestion that EnAds are defective, do not grow on primary human diploid cells, and behave like Ad host-range mutants, i.e., they require early gene products from other Ad types for efficient growth....


    pub_type: 杂志文章


    authors: Pieniazek D,Pieniazek NJ,Macejak D,Coward J,Rayfield M,Luftig RB

    更新日期:1990-01-01 00:00:00

  • Enhanced expression of murine beta-defensins (MBD-1, -2,- 3, and -4) in upper and lower airway mucosa of influenza virus infected mice.

    abstract::Although defensins are known to inhibit the replication of human influenza A virus (IAV) in vitro, their in vivo expression during IAV infection is not known. Here we investigated mRNA and protein expression of several beta-defensins in the airways of IAV infected mice. Expression of murine beta-defensin (MBD)-3 and -...


    pub_type: 杂志文章


    authors: Chong KT,Thangavel RR,Tang X

    更新日期:2008-10-10 00:00:00

  • Host cell p53 associates with the feline calicivirus major viral capsid protein VP1, the protease-polymerase NS6/7, and the double-stranded RNA playing a role in virus replication.

    abstract::p53 is implicated in several cellular pathways such as induction of cell-cycle arrest, differentiation, senescence, and apoptosis. p53 is activated by a broad range of stress signals, including viral infections. While some viruses activate p53, others induce its inactivation, and occasionally p53 is differentially mod...


    pub_type: 杂志文章


    authors: Trujillo-Uscanga A,Gutiérrez-Escolano AL

    更新日期:2020-11-01 00:00:00

  • Do hemagglutinin genes of highly pathogenic avian influenza viruses constitute unique phylogenetic lineages?

    abstract::Avian influenza A viruses of the H5 and H7 subtypes periodically cause severe outbreaks of disease in poultry. The question we wished to address in this study is whether these highly pathogenic strains constitute unique lineages or whether they and related nonpathogenic viruses are derived from common ancestors in the...


    pub_type: 杂志文章


    authors: Röhm C,Horimoto T,Kawaoka Y,Süss J,Webster RG

    更新日期:1995-06-01 00:00:00

  • Nucleic acid species related to the satellite RNA of turnip crinkle virus in turnip plants and virus particles.

    abstract::Multiple RNA species which hybridize to a cloned cDNA probe of the turnip crinkle virus satellite RNA (S-TCV) are found both in virions and in plants infected with virus particles containing S-TCV. At least six of these RNAs appear to be multimeric forms of the satellite RNA based on their migration in denaturing gels...


    pub_type: 杂志文章


    authors: Altenbach SB,Howell SH

    更新日期:1984-04-15 00:00:00

  • The genome sequence of the multinucleocapsid nucleopolyhedrovirus of the Chinese oak silkworm Antheraea pernyi.

    abstract::The complete genome sequence of the Liaoning isolate of the Antheraea pernyi multinucleocapsid nucleopolyhedrovirus (AnpeMNPV) was determined. The viral genome size is 126,246 bp, relatively GC-rich (53.5% G+C), with 145 predicted open reading frames (ORFs) of more than 50 amino acids, accounting for more than 97% of ...


    pub_type: 杂志文章


    authors: Fan Q,Li S,Wang L,Zhang B,Ye B,Zhao Z,Cui L

    更新日期:2007-09-30 00:00:00

  • The equine herpesvirus 1 immediate-early protein interacts with EAP, a nucleolar-ribosomal protein.

    abstract::The equine herpesvirus 1 (EHV-1) immediate-early (IE) phosphoprotein is essential for the activation of transcription from viral early and late promoters and regulates transcription from its own promoter. The IE protein of 1487 amino acids contains a serine-rich tract (SRT) between residues 181 and 220. Deletion of th...


    pub_type: 杂志文章


    authors: Kim SK,Buczynski KA,Caughman GB,O'Callaghan DJ

    更新日期:2001-01-05 00:00:00

  • Inhibition of vaccinia virus replication by N-(phosphonoacetyl)-L-aspartate: differential effects on viral gene expression result from a reduced pyrimidine nucleotide pool.

    abstract::The replication of vaccinia virus was reduced by 3 logs in cells that had been treated before and during infection with a concentration of N-(phosphonoacetyl)-L-aspartate (PALA) which lowered the UTP and CTP to 5 and 20% of controls, respectively, without affecting cell viability. The antiviral activity of PALA was re...


    pub_type: 杂志文章


    authors: Katsafanas GC,Grem JL,Blough HA,Moss B

    更新日期:1997-09-15 00:00:00

  • Transcriptional repression of K-Rta by Kaposi's sarcoma-associated herpesvirus K-bZIP is not required for oriLyt-dependent DNA replication.

    abstract::Kaposi's sarcoma-associated herpesvirus origin-dependent DNA replication requires the core replication proteins plus K-Rta and K-bZIP. To determine which K-bZIP protein domains contribute to oriLyt-dependent DNA replication and facilitate suppression of K-Rta-mediated transcriptional activation, we generated a series ...


    pub_type: 杂志文章


    authors: Rossetto C,Gao Y,Yamboliev I,Papousková I,Pari G

    更新日期:2007-12-20 00:00:00

  • Functional similarities between HIV-1 Tat and DNA sequence-specific transcriptional activators.

    abstract::The Tat regulatory protein encoded by human immunodeficiency virus type 1 (HIV-1) induces high levels of transcription from the viral long terminal repeat (LTR) promoter element after interacting with a promoter proximal RNA target sequence. In the wild-type HIV-1 LTR, this activation is facilitated by the synergistic...


    pub_type: 杂志文章


    authors: Madore SJ,Cullen BR

    更新日期:1995-02-01 00:00:00

  • Identification of an initiator-like element within the HTLV-I promoter.

    abstract::Using the 5' long terminal repeat (LTR) as its only promoter, the HTLV-1 provirus generates a single RNA transcript that undergoes differential splicing to express the various viral proteins. Examination of sequence near the transcription start site revealed an element resembling a transcriptional initiator (Inr) at p...


    pub_type: 杂志文章


    authors: Wycuff DR,Goff MD,Marriott SJ

    更新日期:2001-02-01 00:00:00

  • The spiroplasma virus 4 replicative form cloned in Escherichia coli transfects spiroplasmas.

    abstract::The replicative form (RF) of spiroplasma virus 4 (SpV4) has been purified from infected cells of Spiroplasma melliferum strain G1 by alkaline lysis followed by low melting point agarose gel electrophoresis. A partial restriction map has been established. The circular RF was linearized by cutting at the unique ClaI res...


    pub_type: 杂志文章


    authors: Pascarel-Devilder MC,Renaudin J,Bove JM

    更新日期:1986-06-01 00:00:00

  • The tegument protein UL94 of human cytomegalovirus as a binding partner for tegument protein pp28 identified by intracellular imaging.

    abstract::The tegument protein pp28 of human cytomegalovirus (HCMV) is essential for the assembly of infectious HCMV virions, but how it functions during the process of HCMV tegumentation and envelopment remains unclear. By using live cell fluorescence resonance energy transfer (FRET) microscopy and yeast two-hybrid assays, we ...


    pub_type: 杂志文章


    authors: Liu Y,Cui Z,Zhang Z,Wei H,Zhou Y,Wang M,Zhang XE

    更新日期:2009-05-25 00:00:00

  • Lack of chicken adaptation of newly emergent Eurasian H5N8 and reassortant H5N2 high pathogenicity avian influenza viruses in the U.S. is consistent with restricted poultry outbreaks in the Pacific flyway during 2014-2015.

    abstract::In 2014-2015, the U.S. experienced an unprecedented outbreak of Eurasian clade H5 highly pathogenic avian influenza (HPAI) virus, initially affecting mainly wild birds and few backyard and commercial poultry premises. To better model the outbreak, the pathogenesis and transmission dynamics of representative Eu...


    pub_type: 杂志文章


    authors: Bertran K,Swayne DE,Pantin-Jackwood MJ,Kapczynski DR,Spackman E,Suarez DL

    更新日期:2016-07-01 00:00:00

  • CfMNPV blocks AcMNPV-induced apoptosis in a continuous midgut cell line.

    abstract::Morphological and molecular changes produced by Autographa californica nuclear polyhedrosis virus (AcMNPV) infection in a permissive cell line, IPLB-SF-21AE (SF-21), of Spodoptera frugiperda and a nonpermissive cell line, FPMI-CF-203 (CF-203), of Choristoneura fumiferana are described. CF-203 cells inoculated with AcM...


    pub_type: 杂志文章


    authors: Palli SR,Caputo GF,Sohi SS,Brownwright AJ,Ladd TR,Cook BJ,Primavera M,Arif BM,Retnakaran A

    更新日期:1996-08-01 00:00:00

  • Reduction of soluble dipeptidyl peptidase 4 levels in plasma of patients infected with Middle East respiratory syndrome coronavirus.

    abstract::Dipeptidyl peptidase 4 (DPP4) is a receptor for MERS-CoV. The soluble form of DPP4 (sDPP4) circulates systematically and can competitively inhibit MERS-CoV entry into host cells. Here, we measured the concentration of sDPP4 in the plasma and sputa of 14 MERS-CoV-infected patients of various degrees of disease severity...


    pub_type: 杂志文章


    authors: Inn KS,Kim Y,Aigerim A,Park U,Hwang ES,Choi MS,Kim YS,Cho NH

    更新日期:2018-05-01 00:00:00

  • Correlation between polymerase activity and pathogenicity in two duck H5N1 influenza viruses suggests that the polymerase contributes to pathogenicity.

    abstract::The influenza RNA polymerase is known to be important in pathogenicity and adaptation of avian influenza viruses to mammalian hosts. However, the molecular mechanisms responsible are only partly understood. Here we investigated the role of the polymerase in two different, closely related, H5N1 influenza viruses - a hi...


    pub_type: 杂志文章


    authors: Leung BW,Chen H,Brownlee GG

    更新日期:2010-05-25 00:00:00