Abstract:
:Serial passage of Ross River virus in chick embryo fibroblasts selected for virus variants altered in their reactions to neutralizing monoclonal antibodies. The E1 and E2 genes of each antigenic variant were sequenced; single nucleotide changes were found in E2 leading to amino acid substitutions at either residue 4 (Glu-->Lys) or 218 (Asn-->Lys); no changes were found in the E1 gene. Variants with the substitution at E2 residue 218 replicated less efficiently in 1-day-old mice than did the parental strain. The variant changed at E2 residue 4 showed little alteration in replication efficiency in mice. Similar genotypic or phenotypic changes were not found in virus passaged serially in human or mosquito cell lines.
journal_name
Virologyjournal_title
Virologyauthors
Kerr PJ,Weir RC,Dalgarno Ldoi
10.1006/viro.1993.1143subject
Has Abstractpub_date
1993-03-01 00:00:00pages
446-9issue
1eissn
0042-6822issn
1096-0341pii
S0042-6822(83)71143-8journal_volume
193pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::The guinea pig cytomegalovirus (GPCMV) co-linear gene and potential functional homolog of HCMV UL84 (GP84) was investigated. The GP84 gene had delayed early transcription kinetics and transient expression studies of GP84 protein (pGP84) demonstrated that it targeted the nucleus and co-localized with the viral DNA poly...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.10.028
更新日期:2011-02-05 00:00:00
abstract::The MC80R gene of molluscum contagiosum virus (MCV) type 1 encodes a major histocompatibility complex (MHC) class I homolog that lacks several amino-acid residues critical for peptide binding by MHC molecules, contains an unusually long N-terminal hydrophobic domain possibly derived by triplication of a signal peptide...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9390
更新日期:1998-10-25 00:00:00
abstract::The Chlorella virus PBCV-1 genome is a linear nonpermuted 333-kbp dsDNA molecule with covalently closed hairpin termini. The termini (minus the hairpin) are identical inverted repeats of at least 2185 bases after which the sequence diverges. The inverted repeats contain two small potential open reading frames and seve...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90089-t
更新日期:1991-02-01 00:00:00
abstract::Endogenous retroviruses (ERVs) arise from the infection and integration of past retroviruses into animal hosts. We performed large-scale genomic mining of 101 avian genomes for discovery of ERVs having none-avian origin and investigated the cross-species transmission events. Phylogenetic analysis of the reverse transc...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2019.02.005
更新日期:2019-04-01 00:00:00
abstract::Enveloped virus entry occurs when viral and cellular membranes fuse releasing particle contents into the target cell. Human immunodeficiency virus (HIV) entry occurs by cell-free virus or virus transferred between infected and uninfected cells through structures called virological synapses. We developed a high-through...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2015.10.013
更新日期:2016-03-01 00:00:00
abstract::The subgroup C human adenovirus L4 33-kDa protein is a nuclear phosphoprotein that plays a direct, but dispensable, role in virion assembly. The r-strand open reading frame (ORF) for this protein lies opposite to the 5' end of the l-strand E2 early (E2E) transcription units. To facilitate studies of regulation of E2E ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2001.1130
更新日期:2001-10-25 00:00:00
abstract::The phosphorylated protein pp65 of human Cytomegalovirus (HCMV) is the predominant virion protein and the major tegument constituent. It plays important roles in HCMV infection and virion assembly. Live cell imaging and fluorescence recovery after photobleaching (FRAP) analysis showed that HCMV pp65 accumulated dynami...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.06.021
更新日期:2009-09-30 00:00:00
abstract::The amino acid sequence of BMV (brome mosaic virus) coat protein is presented and compared with the sequence derived from RNA structure. Apart from removal of the N-terminal methionine residue and acetylation of the following serine residue, no post-translational modifications occur. ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(83)90193-9
更新日期:1983-09-01 00:00:00
abstract::Segmented genomes of RNA viruses are thought to evolve and be maintained in analogy to sexual recombination and reassortment in eukaryotic systems. If reassortment among genomes is an important event in cucumoviral evolution, then such events should be detectable among extant viruses. In this study, phylogenetic analy...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.1088
更新日期:1995-02-20 00:00:00
abstract::Polyomavirus (PyV) infection induces protective T-cell-independent (TI) IgM and IgG responses in T-cell-deficient (TCR beta x delta-/-) mice. In this study, we show that PyV is a TI -2 antigen: B cells with a mutated Bruton's tyrosine kinase (Xid mutants) do not respond to PyV with antibody secretion in the absence of...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2000.0766
更新日期:2001-02-15 00:00:00
abstract::In this study, we analyzed the replication and budding sites of severe acute respiratory syndrome coronavirus (SARS-CoV) at early time points of infection. We detected cytoplasmic accumulations containing the viral nucleocapsid protein, viral RNA and the non-structural protein nsp3. Using EM techniques, we found that ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.11.027
更新日期:2007-05-10 00:00:00
abstract::The protein content of vaccinia virus mature virions, purified by rate zonal and isopycnic centrifugations and solubilized by SDS or a solution of urea and thiourea, was determined by the accurate mass and time tag technology which uses both tandem mass spectrometry and Fourier transform-ion cyclotron resonance mass s...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.08.025
更新日期:2007-02-05 00:00:00
abstract::The measles virus (MV) phosphoprotein (P) gene encodes three proteins, P, C, and V. The V protein is synthesized by pseudo-templated transcription, also designated as RNA editing: during P gene transcription one G residue is inserted at a defined position in about 50% of the mRNAs. To study the importance of sequence ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.8339
更新日期:1997-01-20 00:00:00
abstract::During viral entry, enveloped viruses require the fusion of their lipid envelope with host cell membranes. For coronaviruses, this critical step is governed by the virally-encoded spike (S) protein, a class I viral fusion protein that has several unique features. Coronavirus entry is unusual in that it is often biphas...
journal_title:Virology
pub_type: 杂志文章,评审
doi:10.1016/j.virol.2017.12.015
更新日期:2018-04-01 00:00:00
abstract::VP5, a 5'-terminal, small open reading frame in segment A of the aquatic birnavirus (infectious pancreatic necrosis virus, IPNV) genome, encodes a 17-kDa nonstructural protein. We previously reported apoptosis induced by IPNV in a fish cell line. In the present study, we cloned and identified VP5 and tested its functi...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2001.1336
更新日期:2002-04-10 00:00:00
abstract::Vesicular stomatitis virus (VSV) isolated from two independently established lines of persistently infected mouse L cells expressed an altered phenotype of RNA synthesis at 37 degrees, the temperature at which the persistently infected cultures were maintained (T.K. Frey and J.S. Youngner, 1982, J. Virol. 44, 167-174)...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90260-5
更新日期:1984-07-15 00:00:00
abstract::We have examined the host range of AIDS-associated retroviruses (ARV) that are known to infect human T cells of the helper subset. We have observed that the virus cannot infect fibroblast and epithelial cell lines of many different animal species. It is infectious and replicates efficiently in peripheral mononuclear c...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90146-1
更新日期:1985-12-01 00:00:00
abstract::Mannose binding lectin (MBL) generally plays a protective role during viral infection, yet MBL-mediated complement activation promotes Ross River virus (RRV)-induced inflammatory tissue destruction, contributing to arthritis and myositis. As MBL binds to carbohydrates, we hypothesized that N-linked glycans on the RRV ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2017.12.022
更新日期:2018-02-01 00:00:00
abstract::The RTVL-H family of human endogenous retrovirus-like sequences consists of approximately 1000 "full-length" elements and at least as many solitary RTVL-H related long terminal repeats (LTRs). We have characterized cDNA clones from two human cell libraries (Hep-2 and normal peripheral blood) and have found three clone...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90570-9
更新日期:1989-12-01 00:00:00
abstract::Many antiviral drugs must be metabolized to their active form by cellular enzymes. Their antiviral activity may therefore be limited by an inefficient metabolism, leading to low intracellular concentration of the active form or to the accumulation of toxic intermediate metabolites. Gene transfer might be used to overc...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8706
更新日期:1997-09-01 00:00:00
abstract::To clarify the causal relationship between human papillomavirus (HPV) and cervical intraepithelial neoplasia (CIN), we analyzed 386 unfixed biopsy specimens by blot hybridization at Tm -40 degrees C, targeting 38 skin and 42 genital HPVs. By the recognition of PstI, BanI, and MspI cleavage patterns, single genital, bu...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2001.0865
更新日期:2001-04-25 00:00:00
abstract::The flavivirus nonstructural protein NS1 has been implicated in viral RNA replication, although its precise role has not been identified. In its native state NS1 exists as a heat labile homodimer that is thought to be required for NS1 function and secretion. However, we have recently identified a cDNA clone of KUN vir...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1999.9956
更新日期:1999-11-10 00:00:00
abstract::During head assembly of phage T3, DNA is packaged into the cavity of a preformed protein shell, called the prohead, with the aid of noncapsid, packaging proteins, the products of genes 18 and 19 (gp18 and gp19). gp18 and gp19 separately form complexes with DNA and proheads, respectively. These complexes associate to f...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90550-u
更新日期:1991-12-01 00:00:00
abstract::The superinfection exclusion of VSV has been studied and found to be caused by a combination of three distinct effects on endocytosis by VSV-infected cells: first, a decreased rate of formation of endocytic vesicles as judged by an inhibition of fluid-phase uptake at 2 hr postinfection; second, a decreased rate of int...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90494-c
更新日期:1990-07-01 00:00:00
abstract::Missing contact footprinting with formic acid as a modifying reagent was used to examine specific IE-1 binding contacts to double-stranded oligonucleotides that contained either a consensus hr repeat sequence or a sequence from the pe38 promoter, which is down regulated by IE-1. The hr repeat sequences contain two con...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2000.0452
更新日期:2000-08-15 00:00:00
abstract::A number of factors that affect genetic interaction during mixed infection with temperature-sensitive mutants of simian rotavirus SA11 have been examined. (1) Statistical analyses of recombination frequency (RF) indicated that (a) the variability noted in RF was not related to variations in experimental conditions and...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(83)90374-4
更新日期:1983-05-01 00:00:00
abstract::We assess the genetic relationships between 49 HIV-1 group O strains from 24 and 25 patients living in Cameroon and France, respectively. Strains were sequenced in four genomic regions: gag (p24) and three env regions (C2-V3, gp41, and for 22 C2-gp41). In each of the genomic regions analyzed, the genetic diversity amo...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2002.1430
更新日期:2002-10-25 00:00:00
abstract::Sindbis virus (SINV) induces inflammatory and vasoactive responses that are associated with rash and arthritis in human infections. The mechanisms underlying infection-associated microvasculopathy are still unknown. We investigated whether endothelial cells infected by SINV are differentially responsive to bradykinin ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2011.10.003
更新日期:2012-01-05 00:00:00
abstract::High-risk human papillomaviruses (HPVs) are etiologically implicated in human cervical cancer. Two viral genes, E6 and E7, are commonly found expressed in these cancer cells. We have previously shown that mice transgenic for the HPV-16 E6 gene or E7 gene, in which the E6 or E7 was expressed in the basal layer of epith...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1999.0106
更新日期:2000-02-15 00:00:00
abstract::To study the role of human papillomavirus (HPV) infection in the development of genetic instability, we transduced normal human airway and anogenital epithelial cells with various combinations of HPV-16 E6, E7, and the reverse transcriptase component of telomerase (hTERT). Cell lines generated by co-expression of E7 w...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.06.027
更新日期:2005-09-30 00:00:00