Abstract:
:To investigate the mode of gene expression of red clover mottle virus (RCMV) middle component (M) RNA, we have synthesized an oligopeptide corresponding to the predicted carboxy-terminus of the RCMV counterparts of the cowpea mosaic virus (CPMV) 48K and 58K proteins. Using an antiserum raised against this synthetic oligopeptide, we have detected a 43-kDa protein in the 30,000 g pellet from extracts of RCMV-infected cowpea protoplasts. Immunogold cytochemistry further localized this protein to the plasmodesmata of RCMV-infected pea tissue. This subcellular location, taken together with other evidence, suggests that this 43-kDa protein has a role in the cell-to-cell spread of RCMV.
journal_name
Virologyjournal_title
Virologyauthors
Shanks M,Tomenius K,Clapham D,Huskisson NS,Barker PJ,Wilson IG,Maule AJ,Lomonossoff GPdoi
10.1016/0042-6822(89)90552-7subject
Has Abstractpub_date
1989-12-01 00:00:00pages
400-7issue
2eissn
0042-6822issn
1096-0341pii
0042-6822(89)90552-7journal_volume
173pub_type
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