当前位置： SCI文献检索 > VIROLOGY期刊下所有文献 > Early and late functions of alfalfa mosaic virus coat protein can be mutated separately.

Early and late functions of alfalfa mosaic virus coat protein can be mutated separately.

Abstract：

:To investigate the role of alfalfa mosaic virus coat protein (CP) in genome activation, asymmetric plus-strand RNA accumulation, and cell-to-cell spread of the virus, mutations were made in the CP gene and putative CP binding sites in the 3'-untranslated region (UTR) of RNA 3. Mutants that produced no CP-related peptide or CP with an N-terminal deletion of 20 amino acids were defective in all three functions. Insertion of several nonviral amino acids at position 85 of CP had little effect on genome activation and plus-strand RNA accumulation but abolished cell-to-cell spread. A mutant encoding CP with a C-terminal deletion of 21 amino acids was defective in plus-strand RNA accumulation but showed substantial levels of genome activation and cell-to-cell spread. Mutations in the 3'-UTR that interfered with CP binding affected plus-strand RNA accumulation and cell-to-cell spread. Neither CP nor CP binding sites at the 3'-end of RNA 3 were required for minus-strand RNA accumulation. The results demonstrate that early and late functions of CP can be mutated separately, indicating that different domains of CP are involved in the three functions investigated.

journal_name

Virology

journal_title

Virology

authors

van der Vossen EA,Neeleman L,Bol JF

doi

10.1006/viro.1994.1411

subject

Has Abstract

pub_date

1994-08-01 00:00:00

pages

891-903

issue

2

eissn

0042-6822

issn

1096-0341

pii

S0042-6822(84)71411-5

journal_volume

202

pub_type

杂志文章

相关文献

VIROLOGY文献大全
  • Structure of the C-terminal head domain of the fowl adenovirus type 1 short fibre.

    abstract：:There are more than 100 known adenovirus serotypes, including 50 human serotypes. They can infect all 5 major vertebrate classes but only Aviadenovirus infecting birds and Mastadenovirus infecting mammals have been well studied. CELO (chicken embryo lethal orphan) adenovirus is responsible for mild respiratory patholo...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2008.05.011

    authors： El Bakkouri M,Seiradake E,Cusack S,Ruigrok RW,Schoehn G

    更新日期：2008-08-15 00:00:00

  • Comparison of the rotavirus gene 6 from different species by sequence analysis and localization of subgroup-specific epitopes using site-directed mutagenesis.

    abstract：:The nucleotide sequence of gene 6 encoding the rotavirus major capsid protein VP6 of EDIM strain (EW) was determined and compared to that of 20 previously reported strains with known subgroup specificities. Multiple alignments of amino acid sequences exhibited a high level of sequence conservation (87 to 99.2%). Site-...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1997.8762

    authors： Tang B,Gilbert JM,Matsui SM,Greenberg HB

    更新日期：1997-10-13 00:00:00

  • Glycosylation within an antigenic site on the HN glycoprotein of Newcastle disease virus interferes with its role in the promotion of membrane fusion.

    abstract：:The binding of monoclonal antibodies to antigenic site 3 on the hemagglutinin-neuraminidase (HN) glycoprotein of Newcastle disease virus neutralizes viral infectivity and prevents syncytium formation by a mechanism other than the prevention of viral attachment. The virus can escape neutralization by these antibodies b...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1994.1506

    authors： Deng R,Wang Z,Glickman RL,Iorio RM

    更新日期：1994-10-01 00:00:00

  • Mapping of HIV-1 determinants of apoptosis in infected T cells.

    abstract：:HIV-1 infection leads to death of CD4(+) T cells in vivo and in vitro, although the mechanisms of this cell death are not well defined. We used flow cytometry to concurrently analyze infection and apoptosis of the CD4(+) CEM T cell line and human peripheral blood mononuclear cells (PBMC). Surprisingly, T cells product...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1998.9459

    authors： Rapaport E,Casella CR,Ikl,Mustafa F,Isaak D,Finkel TH

    更新日期：1998-12-20 00:00:00

  • Dengue virus NS1 protein interacts with the ribosomal protein RPL18: this interaction is required for viral translation and replication in Huh-7 cells.

    abstract：:Given dengue virus (DENV) genome austerity, it uses cellular molecules and structures for virion entry, translation and replication of the genome. NS1 is a multifunctional protein key to viral replication and pathogenesis. Identification of cellular proteins that interact with NS1 may help in further understanding the...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2015.05.017

    authors： Cervantes-Salazar M,Angel-Ambrocio AH,Soto-Acosta R,Bautista-Carbajal P,Hurtado-Monzon AM,Alcaraz-Estrada SL,Ludert JE,Del Angel RM

    更新日期：2015-10-01 00:00:00

  • Nonstructural NS1 proteins of several mosquito-borne Flavivirus do not inhibit TLR3 signaling.

    abstract：:Flaviviruses are single-stranded positive RNA viruses that replicate through double stranded RNA (dsRNA) intermediates. These dsRNA may be recognized as pathogen-associated molecular patterns by cellular receptors including membrane-bound Toll-like receptor 3 (TLR3) and cytosolic helicases RIG-I and MDA5. dsRNA stimul...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2010.05.020

    authors： Baronti C,Sire J,de Lamballerie X,Quérat G

    更新日期：2010-09-01 00:00:00

  • A major internal initiation site for the in vitro translation of the adenovirus DNA polymerase.

    abstract：:An open reading frame which encodes at least 90% of the adenovirus type 2 DNA polymerase gene was cloned behind the SP6 promoter and transcribed in vitro using the SP6 RNA polymerase. The resultant RNA was translated in a rabbit reticulocyte cell free system. In addition to the translation of a 120-kDa protein corresp...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(86)90181-9

    authors： Hassin D,Korn R,Horwitz MS

    更新日期：1986-11-01 00:00:00

  • Bacteriophage P22 capsid size determination: roles for the coat protein telokin-like domain and the scaffolding protein amino-terminus.

    abstract：:Assembly of icosahedral capsids of proper size and symmetry is not understood. Residue F170 in bacteriophage P22 coat protein is critical for conformational switching during assembly. Substitutions at this site cause assembly of tubes of hexamerically arranged coat protein. Intragenic suppressors of the ts phenotype o...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2011.06.025

    authors： Suhanovsky MM,Teschke CM

    更新日期：2011-09-01 00:00:00

  • Molecular basis of antigenic variation between the glycoproteins C of respiratory bovine herpesvirus 1 (BHV-1) and neurovirulent BHV-5.

    abstract：:Herpesvirus glycoprotein C (gC) functions as a major virus attachment protein. The gC sequence of the neurovirulent bovine herpesvirus type 5 (BHV-5) virus was determined and compared with the gC sequence of the nonneurovirulent BHV-1. Alignment of the predicted amino acid sequences of BHV-1 and BHV-5 gC ORFs showed t...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/s0042-6822(95)80137-5

    authors： Chowdhury SI

    更新日期：1995-11-10 00:00:00

  • Epitope exposure on functional, oligomeric HIV-1 gp41 molecules.

    abstract：:We have used cells infected with the HIV-1 molecular clone HX10 to study the binding of monoclonal antibodies (mAbs) to different epitopes within the extracellular domain of the HIV-1 transmembrane glycoprotein gp41. Gp41 mAb binding to the infected cells at 4 degrees was variable but weaker than the binding of an ant...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/s0042-6822(95)80094-8

    authors： Sattentau QJ,Zolla-Pazner S,Poignard P

    更新日期：1995-01-10 00:00:00

  • Live attenuated measles vaccine expressing HIV-1 Gag virus like particles covered with gp160DeltaV1V2 is strongly immunogenic.

    abstract：:Although a live attenuated HIV vaccine is not currently considered for safety reasons, a strategy inducing both T cells and neutralizing antibodies to native assembled HIV-1 particles expressed by a replicating virus might mimic the advantageous characteristics of live attenuated vaccine. To this aim, we generated a l...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2009.02.047

    authors： Guerbois M,Moris A,Combredet C,Najburg V,Ruffié C,Février M,Cayet N,Brandler S,Schwartz O,Tangy F

    更新日期：2009-05-25 00:00:00

  • Structure of the M2 protein gene of sonchus yellow net virus.

    abstract：:The nucleotide sequence of the gene immediately following the nucleocapsid protein gene of sonchus yellow net virus (SYNV), a plant rhabdovirus, is presented. Serological reactions of SYNV proteins with antibodies elicited by a fusion protein constructed from the sequenced gene indicate that this gene encodes an SYNV ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(87)90190-5

    authors： Heaton LA,Zuidema D,Jackson AO

    更新日期：1987-11-01 00:00:00

  • Maize chlorotic mottle machlomovirus and wheat streak mosaic rymovirus concentrations increase in the synergistic disease corn lethal necrosis.

    abstract：:Corn lethal necrosis (CLN) is caused by the synergistic interaction between maize chlorotic mottle machlomovirus (MCMV) and any potyvirus which infects cereals. Interactions between MCMV and wheat streak mosaic rymovirus (WSMV) in N28Ht corn produced MCMV concentrations that averaged 3.3- to 11.2-fold higher in doubly...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1997.8989

    authors： Scheets K

    更新日期：1998-03-01 00:00:00

  • Towards quality and order in human papillomavirus research.

    abstract：:The International Human Papillomavirus (HPV) Reference Center supports quality and order in HPV research and diagnostics. Notably, the center assigns HPV type numbers to novel HPV types, maintains a reference clone repository, and issues international proficiency panels for HPV genotyping. The established HPV types, c...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2018.04.003

    authors： Mühr LSA,Eklund C,Dillner J

    更新日期：2018-06-01 00:00:00

  • Sequences in the 5' leader of Mason-Pfizer monkey virus which affect viral particle production and genomic RNA packaging: development of MPMV packaging cell lines.

    abstract：:We used a series of deletion mutations in the 5' untranslated region of the prototype D type retrovirus, Mason-Pfizer Monkey Virus (MPMV), to analyse RNA encapsidation. A region was identified upstream of the major splice donor which reduced particle production but had a proportionally greater effect on RNA packaging....

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.2001.1061

    authors： Guesdon FM,Greatorex J,Rhee SR,Fisher R,Hunter E,Lever AM

    更新日期：2001-09-15 00:00:00

  • Rift Valley fever virus NSS gene expression correlates with a defect in nuclear mRNA export.

    abstract：:We investigated the localization of host mRNA during Rift Valley fever virus (RVFV) infection. Fluorescence in situ hybridization revealed that infection with RVFV altered the localization of host mRNA. mRNA accumulated in the nuclei of RVFV-infected but not mock-infected cells. Further, overexpression of the NSS gene...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2015.09.003

    authors： Copeland AM,Van Deusen NM,Schmaljohn CS

    更新日期：2015-12-01 00:00:00

  • Assessment and optimization of rolling circle amplification protocols for the detection and characterization of badnaviruses.

    abstract：:The genus Badnavirus is characterized by members that are genetically and serologically heterogeneous which presents challenges for their detection and characterization. The presence of integrated badnavirus-like sequences in some host species further complicates detection using PCR-based protocols. To address these c...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2019.01.013

    authors： Sukal AC,Kidanemariam DB,Dale JL,Harding RM,James AP

    更新日期：2019-03-01 00:00:00

  • Mutational analysis of the Sendai virus V protein: importance of the conserved residues for Zn binding, virus pathogenesis, and efficient RNA editing.

    abstract：:The V protein of Sendai virus (SeV) is nonessential for virus replication in cell culture but indispensable for viral pathogenicity in mice. At the C terminus of the V protein, there are amino acid residues conserved among the members of the Paramyxovinae subfamily that are clustered in three regions: region I, just d...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.2002.1516

    authors： Fukuhara N,Huang C,Kiyotani K,Yoshida T,Sakaguchi T

    更新日期：2002-08-01 00:00:00

  • Circular dimers of a lambda DNA in infected, nonlysogenic Escherichia coli.

    abstract：:Covalently closed circular dimers of phage lambda DNA have been found in Escherichia coli infected with lambda. These dimers can be formed by either the lambda Red or Int systems, by a nonrecombinational replicative mechanism requiring the activity of the lambda O and P genes or by joining of the cohesive ends. Dimers...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(77)90136-2

    authors： Freifelder D,Baran N,Folkmanis A,Freifelder DL

    更新日期：1977-09-01 00:00:00

  • The structure and cloning of orf virus DNA.

    abstract：:A map of cleavage sites for the restriction endonucleases EcoRI, HindIII, BamHI, HpaI, and KpnI for a New Zealand strain of orf virus (NZ2) DNA has been deduced. Also, the entire genome, apart from approximately 0.1 kbp at each end, has been cloned into various vectors. The genome is 139 kbp in length and, in common w...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(87)90307-2

    authors： Mercer AA,Fraser K,Barns G,Robinson AJ

    更新日期：1987-03-01 00:00:00

  • The high levels of p53 present in adenovirus early region 1-transformed human cells do not cause up-regulation of MDM2 expression.

    abstract：:The cellular protein MDM2 can bind to the tumor suppressor gene product p53 and abrogate its transcriptional activity. In addition, p53 can regulate expression of the mdm2 gene. We and others have previously shown that p53 is present at high levels in adenovirus-transformed cells which express the larger E1B protein. ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1995.1349

    authors： Grand RJ,Lecane PS,Owen D,Grant ML,Roberts S,Levine AJ,Gallimore PH

    更新日期：1995-07-10 00:00:00

  • Deletion analysis of a replication origin of human cytomegalovirus by a novel assay system with a combination of microinjection and polymerase chain reaction.

    abstract：:We have developed a sensitive assay for DNA replication in mammalian cells that enables us to detect replicated DNA fragments of human cytomegalovirus. A 1/100 portion of DNA extracted from 1000 microinjected cells was subjected to polymerase chain reaction after digestion with appropriate restriction enzymes to diffe...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1993.1038

    authors： Watanabe S,Yamaguchi N

    更新日期：1993-01-01 00:00:00

  • Efficient inoculation with CaMV 35 S promoter-driven DNA clones of the tobravirus PEBV.

    abstract：:Clones have been constructed containing full-length cDNA copies of PEBV RNA1 and RNA2, flanked by the CaMV 35 S RNA promoter and the nopaline synthase terminator. The clones are infectious when inoculated onto Nicotiana benthamiana plants. Both the viral RNAs and the virus particles were identified in infected plants....

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(92)90488-b

    authors： MacFarlane SA,Gilmer D,Davies JW

    更新日期：1992-04-01 00:00:00

  • Coexpression of the adenovirus 12 E1B 55 kDa oncoprotein and cellular tumor suppressor p53 is sufficient to induce metaphase fragility of the human RNU2 locus.

    abstract：:Adenovirus 12 (Ad12), but not adenovirus 2 or 5, induces metaphase chromosome fragility at four specific loci in humans: RNU1, RNU2, PSU1, and RN5S. As each of these sites corresponds to a tandemly repeated multigene family encoding a small, abundant structural RNA, we proposed that Ad12 hinders metaphase chromatin co...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1998.9512

    authors： Liao D,Yu A,Weiner AM

    更新日期：1999-02-01 00:00:00

  • Genetic variation and microevolution of dengue 2 virus in Southeast Asia.

    abstract：:Dengue 2 (DEN 2) virus strains collected from dengue hemorrhagic fever (DHF) patients and Aedes aegypti mosquitoes in Thailand, Burma, and Vietnam over a 25-year period have been analyzed by computer assisted T1-RNase-resistant oligonucleotide fingerprinting. Fifty-seven DEN 2 virus strains of the Thailand topotype we...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(89)90195-5

    authors： Trent DW,Grant JA,Monath TP,Manske CL,Corina M,Fox GE

    更新日期：1989-10-01 00:00:00

  • HIV infection of the BS-1 human stroma cell line: effect on murine hematopoiesis.

    abstract：:BS-1, a stroma cell line derived from normal human bone marrow, can support the growth of murine erythroid (BFU-E), granulocyte-macrophage (CFU-GM), and megakaryocyte (CFU-Meg) progenitor cells in a short-term in vitro coculture system. Exposure of BS-1 cell to the human immunodeficiency virus (HIV) prior to coculture...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1993.1159

    authors： Steinberg HN,Anderson J,Crumpacker CS,Chatis PA

    更新日期：1993-03-01 00:00:00

  • A highly pathogenic porcine reproductive and respiratory syndrome virus generated from an infectious cDNA clone retains the in vivo virulence and transmissibility properties of the parental virus.

    abstract：:The nucleotide sequence of a highly pathogenic porcine reproductive and respiratory syndrome virus (PRRSV) was determined. Transfection of MARC-145 cells with capped in vitro transcripts derived from a full-length cDNA clone of the viral genome resulted in infectious PRRSV with growth characteristics similar to that o...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2004.04.046

    authors： Truong HM,Lu Z,Kutish GF,Galeota J,Osorio FA,Pattnaik AK

    更新日期：2004-08-01 00:00:00

  • Limitation of Tat-associated transcriptional processivity in HIV-infected PBMC.

    abstract：:The ability of HIV to match levels of viral mRNA to the activation state of the host cell may play a role in its ability to persist as well as to replicate. This linkage depends on the function of the viral transcriptional regulatory protein, Tat, which increases the efficiency of RNA elongation (transcriptional proce...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1999.9647

    authors： Adams M,Wong C,Wang D,Romeo J

    更新日期：1999-05-10 00:00:00

  • Antibody response in the female rabbit reproductive tract to influenza haemagglutinin encoded by a recombinant myxoma virus.

    abstract：:The antibody response in serum and the reproductive tract of female rabbits to a model antigen, influenza virus haemagglutinin (HA), encoded by a recombinant myxoma virus was investigated. Strong and lasting IgG antibody responses to HA were induced in serum following intradermal, intranasal, and intravaginal immunisa...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/s0042-6822(03)00324-6

    authors： Gu W,Holland M,Janssens P,Kerr P

    更新日期：2003-08-15 00:00:00

  • Enteric adenovirus type 40:E1B transcription map and identification of novel E1A-E1B cotranscripts in lytically infected cells.

    abstract：:Adenovirus 40 (Ad40) is defective for growth in tissue culture but is complemented when the Ad2/5 or Ad12 E1B 55K protein is supplied in trans. Ad40 E1B mRNA has not been detected in E1-transformed cells, or at early times in lytically infected cells. In cells constitutively expressing the E1B region of Ad2, Ad40 E1B ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(91)90478-t

    authors： Steinthorsdottir V,Mautner V

    更新日期：1991-03-01 00:00:00