Abstract：
:In HSV types 1 and 2, gH forms a noncovalent heterodimer with gL. Previous studies demonstrated that the first 323 amino acids of gH1 and the first 161 amino acids of gL1 are sufficient for gH/gL binding. For gL1, substitution of any of its four cysteine (C) residues (all located within the gH/gL binding region) destroyed gH binding and function. Although gH1 contains 8 cysteines in its ectodomain, gH 2 contains 7 (C3 of gH1 is replaced by arginine in gH2). We found that mutation of any of the four C-terminal cysteines led to a reduction or loss of gH/gL function. Mutation of C5 or C6 in gH1 or gH2 rendered the proteins non-functional. However, substitution of C7 and/or C8 in gH1 has a definite negative impact on cell-cell fusion, although these mutations had less effect on complementation. Remarkably, all four gH1 N-terminal cysteines could be mutated simultaneously with little effect on fusion or complementation. As gH2 already lacks C3, we constructed a triple mutant (gH2-C1/2/4) which exhibited a similar phenotype. Since gH1 is known to bind gL2 and vice versa, we wondered whether binding of gH2 to the heterologous gL1 would enhance the fusion defect seen with the gH2-C2 mutant. The combination of mutant gH2-C2 with wild-type gL1 was nonfunctional in a cell-cell fusion assay. Interestingly, the reciprocal was not true, as gH1-C2 could utilize both gL1 and gL2. These findings suggest that there is a structural difference in the gH2 N-terminus as compared to gH1. We also present genetic evidence for at least one disulfide bond within gH2, between cysteines 2 and 4.
journal_name
Virologyjournal_title
Virologyauthors
Cairns TM,Landsburg DJ,Whitbeck JC,Eisenberg RJ,Cohen GHdoi
10.1016/j.virol.2004.12.006subject
Has Abstractpub_date
2005-02-20 00:00:00pages
550-62issue
2eissn
0042-6822issn
1096-0341pii
S0042-6822(04)00820-7journal_volume
332pub_type
杂志文章相关文献
VIROLOGY文献大全abstract：:Human papilllomaviruses (HPVs) are common human pathogens that infect cutaneous or mucosal epithelia in which they cause warts, self-contained benign lesions that commonly regress. The HPV life cycle is intricately tied to the differentiation of the host epithelium it infects. Mucosotropic HPVs are the most common sex...
journal_title：Virology
pub_type： 杂志文章
doi：10.1016/j.virol.2013.07.016
更新日期：2013-10-01 00:00:00
abstract：:Infections of nonpermissive ( sup0 ) Escherichia coli by T1 phage with amber mutations in either gene 3.5 or gene 4 exhibit a variety of defective phenotypes, including premature arrest of T1 DNA synthesis, failure to make concatemeric DNA, formation of an abnormal DNA replication intermediate, failure to package phag...
journal_title：Virology
pub_type： 杂志文章
doi：10.1016/0042-6822(84)90130-2
更新日期：1984-05-01 00:00:00
abstract：:Hantaviruses are enveloped RNA viruses that belong to the family Bunyaviridae. They are the causative agents of hemorrhagic fever with renal syndrome (HFRS) and hantavirus pulmonary syndrome (HPS). Hantaviruses show a worldwide distribution with specific rodent species as natural hosts. It is known that rodents can tr...
journal_title：Virology
pub_type： 杂志文章
doi：10.1016/j.virol.2003.09.006
更新日期：2004-01-05 00:00:00
abstract：:A new strain of wound tumor virus (WTV) has been isolated from a periwinkle plant (Catharanthus roseus) that was among several used as bait plants in a blueberry field. The 12 segments of double-stranded RNA of the viral genome were isolated directly from infected tissue and found to have mobilities through agarose ge...
journal_title：Virology
pub_type： 杂志文章
doi：10.1016/0042-6822(91)90568-v
更新日期：1991-12-01 00:00:00
abstract：:The most recent introduction of an avian influenza A virus without reassortment into mammals occurred in 1979 when H1N1 strains could be isolated from diseased pigs in northern Europe. This newly introduced avian virus formed a stable lineage in pigs and, in the meantime, spread all over Europe. In 1991 highly pathoge...
journal_title：Virology
pub_type： 杂志文章
doi：10.1006/viro.1994.1344
更新日期：1994-07-01 00:00:00
abstract：:Ribavirin is the first synthetic, broad-spectrum antiviral nucleoside. Despite its more than 30 year history, the mechanism of action of this compound remains unclear and somewhat controversial. Recent data suggest the possibility that the activity of ribavirin against RNA viruses is a reflection of incorporation of r...
journal_title：Virology
pub_type： 杂志文章,评审
doi：10.1006/viro.2002.1487
更新日期：2002-07-05 00:00:00
abstract：:The 30K protein of tobacco mosaic virus was recently confirmed to be involved in cell-to-cell movement of the virus. To characterize common structural features of the 30K protein, the nucleotide sequence of the 30K protein gene of cucumber green mottle mosaic virus (CGMMV, a member of the tobamoviruses) RNA has been d...
journal_title：Virology
pub_type： 杂志文章
doi：
更新日期：1988-12-01 00:00:00
abstract：:Since SARS-CoV-2 spreads rapidly around the world, data have been needed on the natural fluctuation of viral load and clinical indicators associated with it. We measured and compared viral loads of SARS-CoV-2 from pharyngeal swab, IgM anti-SARS-CoV-2, CRP and SAA from serum of 114 COVID-19 patients on admission. Posit...
journal_title：Virology
pub_type： 杂志文章
doi：10.1016/j.virol.2020.04.011
更新日期：2020-07-01 00:00:00
abstract：:We have previously shown that peroxisome proliferator-activated receptor-gamma (PPARgamma) agonists inhibited the inflammatory response of RSV-infected human lung epithelial cells. In this study, we supply evidence that specific PPARgamma agonists (15d-PGJ2, ciglitazone, troglitazone, Fmoc-Leu) efficiently blocked the...
journal_title：Virology
pub_type： 杂志文章
doi：10.1016/j.virol.2006.03.008
更新日期：2006-07-05 00:00:00
abstract：:The 85-kDa polypeptide previously shown to be associated with the restricted (R) component of the EBV-induced early antigen (EA) complex was subjected to amino acid sequencing analysis. This was accomplished by cyanogen bromide cleavage and by separation of individual peptides by high-pressure liquid chromatography em...
journal_title：Virology
pub_type： 杂志文章
doi：10.1016/0042-6822(87)90331-x
更新日期：1987-03-01 00:00:00
abstract：:A procedure was developed for the purification of the pea enation mosaic virus (PEMV) using differential and rate zonal centrifugation in sucrose density gradients. Infectivity and serological data indicated that the highest virus concentration occurs in infected peas (Pisum sativum) 10-12 days after mechanical inocul...
journal_title：Virology
pub_type： 杂志文章
doi：10.1016/0042-6822(66)90060-2
更新日期：1966-03-01 00:00:00
abstract：:Baculoviruses encode fibroblast growth factor (vfgf) homologs whose function during virus infection is unknown. We constructed a recombinant bacmid of Autographa californica M nucleopolyhedrovirus (AcMNPV) lacking a functional vfgf and characterized it in two insect cell lines. The kinetics of budded virus production ...
journal_title：Virology
pub_type： 杂志文章
doi：10.1016/j.virol.2006.01.016
更新日期：2006-03-15 00:00:00
abstract：:To illuminate the molecular basis for host range restriction of parainfluenza virus replication, we have examined the types of virus macromolecules produced during abortive infection of nonpermissive MDBK cells with human parainfluenza virus type 1 (hPIV1). While these cells do not support production of hPIV1 virus, t...
journal_title：Virology
pub_type： 杂志文章
doi：10.1006/viro.1996.0287
更新日期：1996-06-01 00:00:00
abstract：:Induction with ultraviolet light of K12 lysogens carrying defective prophages leads to death of the host cell. In order to determine whether the killing accompanying induction was due to diffusible phage products, or was a result of prophage excision itself, the survival of lysogens carrying DNA defective prophages on...
journal_title：Virology
pub_type： 杂志文章
doi：10.1016/0042-6822(68)90012-3
更新日期：1968-01-01 00:00:00
abstract：:The influenza A virus nucleoprotein previously expressed in Escherichia coli after fusion to 32 heterologous amino acids has now been purified and tested for its ability to form complexes with RNA in vitro. By using a simple filter binding assay, we show that ribonucleoprotein (RNP) complexes form readily with single-...
journal_title：Virology
pub_type： 杂志文章
doi：10.1016/0042-6822(87)90419-3
更新日期：1987-02-01 00:00:00
abstract：:The nucleotide and deduced amino acid sequence of G serotype 3 equine rotavirus strain H-2 was determined. A predicted 776-amino-acid H-2 VP4 shows less than or equal to 85.3% identity to other rotavirus VP4 types sequenced to date and thus represents a new P serotype. A PCR-generated probe derived from a cDNA clone o...
journal_title：Virology
pub_type： 杂志文章
doi：10.1006/viro.1993.1152
更新日期：1993-03-01 00:00:00
abstract：:Envelope viruses maturate by macromolecule assembly and budding. To investigate these steps, we generated virus-like particles (VLPs) by co-expression of structural proteins of Sendai virus (SeV), a prototype of the family Paramyxoviridae. Simultaneous expression of matrix (M), nucleo- (N), fusion (F), and hemagglutin...
journal_title：Virology
pub_type： 杂志文章
doi：10.1016/j.virol.2004.04.019
更新日期：2004-07-20 00:00:00
abstract：:The utilization of transgenic plants expressing recombinant antigens to be used in the formulation of experimental immunogens has been recently communicated. We report here the development of transgenic plants of alfalfa expressing the structural protein VP1 of foot and mouth disease virus (FMDV). The presence of the ...
journal_title：Virology
pub_type： 杂志文章
doi：10.1006/viro.1998.9590
更新日期：1999-03-15 00:00:00
abstract：:Among human papillomavirus (HPV) types, clinical association with benign vs malignant lesions correlates with the ability of the corresponding oncogenes to transform cells in vitro. However, even though HPV-11 is considered a low-risk type, we have reported previously that the E5a oncogene of HPV-11gt is capable of tr...
journal_title：Virology
pub_type： 杂志文章
doi：10.1006/viro.1996.0279
更新日期：1996-06-01 00:00:00
abstract：:cDNA clones encoding the envelope glycoprotein E1 of rubella virus (RV) were altered by site-directed mutagenesis at consensus sites for addition of N-linked glycans. The resulting plasmids were introduced into COS cells and the mutant E1 proteins were analyzed by indirect immunofluorescence, radioimmunoprecipitation,...
journal_title：Virology
pub_type： 杂志文章
doi：10.1016/0042-6822(91)90915-x
更新日期：1991-04-01 00:00:00
abstract：:Recent studies have shown enhanced antitumor efficacy with adenoviruses that either lack E1B-19K or overexpress E3-11.6K (also known as adenoviral death protein). E1B-19K is a well-characterized anti-apoptotic protein, and viruses with E1B-19K deletions show increased cytopathicity. However, the mechanism of cell kill...
journal_title：Virology
pub_type： 杂志文章
doi：10.1016/j.virol.2004.06.007
更新日期：2004-09-01 00:00:00
abstract：:Human parainfluenza virus type 2 phosphoprotein (P) is an essential component of viral polymerase. The P gene encodes both P and accessory V proteins by a specific gene editing mechanism. Therefore, the N-terminal 164 amino acids of P protein are common to V protein. Interestingly, while P protein is located in the cy...
journal_title：Virology
pub_type： 杂志文章
doi：10.1016/j.virol.2018.12.005
更新日期：2019-02-01 00:00:00
abstract：:Human infection with H7N9 virus has provoked global public health concern due to the substantial morbidity and mortality. Neuraminidase inhibitors (NAIs) are used as first-line drugs to treat the infection. However, virus quasispecies can evolve rapidly under drug pressure, which may alter various biological character...
journal_title：Virology
pub_type： 杂志文章
doi：10.1016/j.virol.2019.08.004
更新日期：2019-10-01 00:00:00
abstract：:Dengue virus (DENV) is an arbovirus that belongs to the Flaviviridae family. Studies reveal that peptides secreted by amphibians have many functions, such as antiviral and antimicrobial activities. As there is no antiviral drug effective against the DENV, the antiviral activity of a synthetic peptide called HS-1, deri...
journal_title：Virology
pub_type： 杂志文章
doi：10.1016/j.virol.2017.11.009
更新日期：2018-01-15 00:00:00
abstract：:The major and minor nucleoprotein components in turnip yellow mosaic virus (TYMV) preparations have been fractionated by four cycles of CsCl gradient equilibrium centrifugations. RNAs isolated from the various fractions were translated in a rabbit reticulocyte cell-free system. Three classes of RNAs were studied: geno...
journal_title：Virology
pub_type： 杂志文章
doi：10.1016/0042-6822(79)90170-3
更新日期：1979-07-15 00:00:00
abstract：:The envelope glycoprotein E2 of hepatitis C virus (HCV) is a major component of the viral envelope. Knowledge of its topologic features and antigenic determinants in virions is crucial in understanding the viral binding sites to cellular receptor(s) and the induction of neutralizing antibodies. The lack of a robust ce...
journal_title：Virology
pub_type： 杂志文章
doi：10.1006/viro.2002.1463
更新日期：2002-06-20 00:00:00
abstract：:Human nectin-1 (HveC, Prr1), a member of the immunoglobulin superfamily and a receptor for the entry of herpes simplex viruses 1 and 2 (HSV-1, HSV-2), pseudorabies virus (PRV), and bovine herpesvirus 1 (BHV-1), binds to viral gD. For HSV-1, HSV-2, and PRV, the gD-binding region of nectin-1 has been localized to the N-...
journal_title：Virology
pub_type： 杂志文章
doi：10.1006/viro.2001.0989
更新日期：2001-07-05 00:00:00
abstract：:The filoviruses Ebolavirus (EBOV) and Marburgvirus (MARV) cause severe hemorrhagic fever in humans and are potential agents of biological warfare. The envelope glycoprotein (GP) of filoviruses mediates viral entry into cells and is an attractive target for therapeutic intervention and vaccine design. Here, we asked if...
journal_title：Virology
pub_type： 杂志文章
doi：10.1016/j.virol.2006.04.038
更新日期：2006-09-01 00:00:00
abstract：:A previous study suggested that the genomes of the arenaviruses native to North America are a product of genetic recombination between New World arenaviruses with significantly different phylogenetic histories. The purpose of this study was to extend our knowledge of the principal host relationships and evolutionary h...
journal_title：Virology
pub_type： 杂志文章
doi：10.1016/j.virol.2007.05.031
更新日期：2007-10-25 00:00:00
abstract：:The measles virus (MV) phosphoprotein (P) and V proteins block the interferon (IFN) response by impeding phosphorylation of the signal transducer and activator of transcription 1 (STAT1) by the Janus kinase 1 (JAK1). We characterized how STAT1 mutants interact with P and JAK1 phosphorylation. Certain mutants of the li...
journal_title：Virology
pub_type： 杂志文章
doi：10.1016/j.virol.2013.06.019
更新日期：2013-09-01 00:00:00
