Abstract:
:Among human papillomavirus (HPV) types, clinical association with benign vs malignant lesions correlates with the ability of the corresponding oncogenes to transform cells in vitro. However, even though HPV-11 is considered a low-risk type, we have reported previously that the E5a oncogene of HPV-11gt is capable of transforming NIH 3T3 cells in culture. In this study, we found that HPV-11gt E5a and E6 oncogenes have the ability to transform NIH 3T3 and the rat embryo fibroblast line REF 52. Cells were transfected independently with expression plasmids containing the HPV-11gt E5a or E6 oncogenes or both plasmids simultaneously to examine potential interactions. Cells containing these plasmids were phenotypically transformed and had an accelerated doubling time, loss of contact inhibition of growth, and loss of anchorage dependence for cell division. Independent cell lines containing the HPV-11gt E6 gene exhibited variable levels of phenotypic transformation that correlated with the HPV-11gt E6 gene content. The degree of phenotypic transformation could be increased by elevating the level of transcription of the E6 gene, indicating that there is a dose response effect for transformation in this system. These results suggest that increased expression of E6 may be an important factor in malignant progression of naturally occurring tumors.
journal_name
Virologyjournal_title
Virologyauthors
Trujillo JM,Mounts Pdoi
10.1006/viro.1996.0279subject
Has Abstractpub_date
1996-06-01 00:00:00pages
1-9issue
1eissn
0042-6822issn
1096-0341pii
S0042-6822(96)90279-2journal_volume
220pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::The HIV-2 viral accessory protein Vpx is related to, but distinct from the Vpr protein of HIV-1. Vpx is packaged into virions and as a component of the viral preintegration complex (PIC) is required for efficient virus replication in non-dividing cells. We have previously reported that the minimal transferable region ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.10.036
更新日期:2006-03-01 00:00:00
abstract::We used a mouse nasal model of herpes simplex virus 2 (HSV-2) infection to examine the biological properties of HSV-2 wild-type (wt), TK-negative, and replication-defective strains in vivo. Nasal septa tissue is the major site of wt viral replication post intranasal (i.n.) inoculation. The HSV-2 strain 186 syn(+)-1 wt...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2000.0628
更新日期:2000-12-05 00:00:00
abstract::The envelope (E) protein of dengue virus (DENV) is composed of three domains (EDI, EDII, EDIII) and is the main target of neutralizing antibodies. Many monoclonal antibodies that bind EDIII strongly neutralize DENV. However in vitro studies indicate that anti-EDIII antibodies contribute little to the neutralizing pote...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2012.03.003
更新日期:2012-07-20 00:00:00
abstract::The HPV16 E7 oncoprotein and 17β-estradiol are important factors for the induction of premalignant lesions and cervical cancer. The study of these factors is crucial for a better understanding of cervical tumorigenesis. Here, we assessed the global gene expression profiles induced by the HPV16 E7 oncoprotein and/or 17...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2013.08.036
更新日期:2013-12-01 00:00:00
abstract::We have investigated a novel strategy for coexpressing two genes from a retroviral vector. The 5' nontranslated leader region of at least some picornavirus RNAs contains a sequence that can act as an internal ribosome entry site allowing initiation of translation at a downstream AUG codon in a 5' cap-independent manne...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90033-l
更新日期:1992-02-01 00:00:00
abstract::Hepatitis C virus (HCV) NS5A is a phosphoprotein that possesses a cryptic trans-activation activity. To investigate its potential role in viral replication, we searched for the cellular proteins interacting with NS5A protein by yeast two-hybrid screening of a human hepatocyte cDNA library. We identified a newly discov...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1999.9893
更新日期:1999-10-10 00:00:00
abstract::The E7 oncoprotein of high risk human papillomavirus type 16 (HPV16) binds and inactivates the retinoblastoma (RB) family of proteins. Our previous studies suggested that HPV16 E7 enters the nucleus via a novel Ran-dependent pathway independent of the nuclear import receptors (Angeline, M., Merle, E., and Moroianu, J....
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.09.037
更新日期:2009-01-05 00:00:00
abstract::The bacteriophage T4 e gene encodes lysozyme (e-lysozyme), which releases progeny phage after normal infection of Escherichia coli cells. A mutation in the spackle gene suppresses the defect in e-lysozyme (Emrich, 1968). The spackle gene was mapped between genes 41 and 61, but its precise location has not previously b...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1999.9829
更新日期:1999-08-01 00:00:00
abstract::Inhibition of cellular respiration, oxidation of glutathione and induction of apoptosis have been reported in epithelial cells infected in vitro with influenza A virus (IAV). Here, the same biomarkers were investigated in vivo by assessing the lungs of BALB/c mice infected with IAV. Cellular respiration declined on da...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2013.07.034
更新日期:2013-11-01 00:00:00
abstract::Callitrichid hepatitis (CH) is a highly fatal, rodent-borne zoonosis of New World primates (family Callitrichidae) caused by lymphocytic choriomeningitis virus (LCMV). It is unclear whether virulence in Callitrichidae is associated with specific genetic or phylogenetic markers of the virus as only a partial S RNA sequ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2001.0909
更新日期:2001-06-05 00:00:00
abstract::Following primary ocular infection, HSV-1 establishes a latent infection in the trigeminal ganglia with the complete absence of detectable infectious virus. Recently, we showed that vaccination of BALB/cJ with HSV-1 glycoprotein K (gK), but not other HSV-1 glycoproteins, significantly exacerbated the severity of HSV-1...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0537
更新日期:1996-10-01 00:00:00
abstract::The full-length E2 protein of human papillomavirus type 16 is believed to act as a trans-repressor of the viral p97 promoter. Previous reports have provided evidence that transcripts with the potential to encode the E2 protein contain the 880/2708 splice junction. We have further analyzed the structure of the E2-encod...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90271-p
更新日期:1992-12-01 00:00:00
abstract::A class of disulfide constrained peptides containing a core motif FPWG was identified from a screen of phage displayed library using the HCV RNA-dependent RNA polymerase (NS5B) as a bait. Surface plasmon resonance studies showed that three highly purified synthetic constrained peptides bound to immobilized NS5B with e...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(03)00313-1
更新日期:2003-08-15 00:00:00
abstract::Previous reports from our lab had shown that sera obtained from SIV(mac)-infected animals neutralized SIV(mac) infectivity in CD4(+) T cells but failed to protect monkey primary macrophages from infection with the virus. However, the antibodies could inhibit completion of the viral life cycle in the macrophages at the...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2001.1053
更新日期:2001-09-01 00:00:00
abstract::Proinflammatory cytokines and chemokines are often detected in brain tissue of patients with neurological diseases such as multiple sclerosis (MS), HIV-associated dementia (HAD) and Alzheimer's disease (AD). We have utilized a mouse model of retrovirus-induced neurological disease to examine how these proinflammatory ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.06.016
更新日期:2006-10-10 00:00:00
abstract::The recent development of arterivirus full-length cDNA clones makes possible the construction of chimeric arteriviruses for fundamental and applied studies. Using an equine arteritis virus (EAV) infectious cDNA clone, we have engineered chimeras in which the ectodomains of the two major envelope proteins, the glycopro...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2001.1074
更新日期:2001-09-30 00:00:00
abstract::The International Human Papillomavirus (HPV) Reference Center supports quality and order in HPV research and diagnostics. Notably, the center assigns HPV type numbers to novel HPV types, maintains a reference clone repository, and issues international proficiency panels for HPV genotyping. The established HPV types, c...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2018.04.003
更新日期:2018-06-01 00:00:00
abstract::Primary duck hepatocytes maintained in serum-free culture medium containing dimethyl sulfoxide support efficient replication of duck hepatitis B virus following infection in vitro. Cells remain susceptible to infection for at least 2 weeks after plating, allowing spread of virus via repeat cycles of infection. Up to 1...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90199-2
更新日期:1989-10-01 00:00:00
abstract::During replication, the lengthy genome of dsDNA viruses is translocated with remarkable velocity into the limited space within the preformed procapsid. We previously found that a viral-encoded RNA (pRNA) played a key role in bacterial virus phi29 DNA translocation. Design of mutant pRNA sets containing two and three i...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2000.0771
更新日期:2001-03-15 00:00:00
abstract::Although many workers have investigated the maturation and processing of the flavivirus nonstructural glycoprotein NS1 in infected cells, these studies have provided little insight into a possible function for NS1. In this study we investigated the subcellular localization of NS1 both by immunofluorescence and cryo-im...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0307
更新日期:1996-06-01 00:00:00
abstract::A novel human papillomavirus type (HPV) was cloned from an invasive cervical carcinoma. The viral clone showed no homology with other known prototypes of HPV (HPV-1 through HPV-57), except HPV-33 by Southern blot analysis under stringent conditions. It showed less than 20% homology to HPV-33 by reassociation kinetic a...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90560-e
更新日期:1990-08-01 00:00:00
abstract::In this study we report the development and optimization of two minigenome rescue systems for Nipah virus, a member of the Paramyxoviridae family. One is mediated by the T7 RNA polymerase supplied either by a constitutively expressing cell line or by transfection of expression plasmids and is thus independent from inf...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2007.08.008
更新日期:2008-01-05 00:00:00
abstract::The bovine herpesvirus 1 (BHV-1) U(S) ORF8 protein with homology to the Us9 protein of other alphaherpesviruses induces apoptosis in rabbit kidney (RK13) cells without the presence of other BHV-1-encoded proteins. In this article, we have characterized the cytotoxicity and growth behavior of a BHV-1 recombinant, BHV-1...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2002.1656
更新日期:2002-12-05 00:00:00
abstract::To gain new insights regarding the role of Ku, the DNA-PK DNA-binding component, during lentiviral DNA integration, we have investigated the HIV-1 replication in Ku80-depleted human cells. CEM4fx cells underexpressing the Ku80 factor were selected after transduction by a retroviral vector expressing the Ku80 full-leng...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2002.1515
更新日期:2002-08-15 00:00:00
abstract::HPV-16 is the major causes of cervical cancer. Persistence of infection is a necessary event for progression of the infection to cancer. Among other factors, virus persistence is due the viral proteins fighting the immune response. HPV-16 E5 down-regulates MHC/HLA class I, which is much reduced on the cell surface and...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.07.044
更新日期:2010-11-10 00:00:00
abstract::We describe here the transduction of murine hematopoietic progenitor cells with the dominant selectable neomycin drug-resistance (Neo) gene using a recombinant adeno-associated virus (AAV) vector. Successful transformation of progenitor cells to drug resistance was determined to be approximately 1.5% by colony formati...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90491-6
更新日期:1988-02-01 00:00:00
abstract::We have previously shown that the Edmonston strain of measles virus enters and is released preferentially at the apical surfaces of polarized epithelial cells. Small amounts of virus were found to be released at the basal surface. In the present study, we passaged the virus in polarized cells and characterized the pas...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8520
更新日期:1997-05-12 00:00:00
abstract::Information about the basic biological properties of human rhinovirus-C (HRV-C) viruses is lacking due to difficulties with culturing these viruses. Our objective was to develop a cell culture system to grow HRV-C. Epithelial cells from human sinuses (HSEC) were differentiated at air-liquid interface (ALI). Differenti...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2012.11.002
更新日期:2013-02-05 00:00:00
abstract::Glycoprotein C (gC) of pseudorabies virus, a swine herpesvirus, initiates virus attachment by binding to heparan sulfate (HS) linked to proteoglycans (HSPGs) on the cell surface. This interaction facilitates a required step in virus entry, the binding to a non-HS coreceptor, likely by another viral glycoprotein, gD. W...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(02)00024-7
更新日期:2003-03-01 00:00:00
abstract:BACKGROUND:SARS-CoV-2 is a novel coronavirus and the cause of COVID-19. More than 80% of COVID-19 patients exhibit mild or moderate symptoms. In this study, we investigated the dynamics of viral load and antibodies against SARS-CoV-2 in a longitudinal cohort of COVID-19 patients with severe and mild/moderate diseases. ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2020.09.008
更新日期:2020-12-01 00:00:00