Establishment and characterization of plasmid-driven minigenome rescue systems for Nipah virus: RNA polymerase I- and T7-catalyzed generation of functional paramyxoviral RNA.

abstract：:Infection with many viruses results in the selective shutoff of host protein synthesis. A common target for virus interference with host protein synthesis is the cap-binding protein complex, eIF4F. The large subunit of the complex, eIF4G, is cleaved upon picornavirus (except cardiovirus) infection. Infection with aden...

journal_title：Virology

authors： Gingras AC,Sonenberg N

更新日期：1997-10-13 00:00:00

abstract：:Published ultraviolet (uv) inactivation data and in vitro transcription studies have suggested that vesicular stomatitis virus (VSV) leader RNA was solely responsible for the inhibition of host cell RNA synthesis by this virus. Since no protein product is encoded in leader RNA, this conclusion implied that no protein ...

journal_title：Virology

authors： Poirot MK,Schnitzlein WM,Reichmann ME

更新日期：1985-01-15 00:00:00

abstract：:After intranasal inoculation, mouse hepatitis virus (MHV) gains entry into the central nervous system (CNS) via the olfactory and trigeminal nerves. Under the appropriate conditions, some mice develop clinically apparent demyelinating encephalomyelitis several weeks later, with virus always present in the spinal cord....

journal_title：Virology

authors： Perlman S,Jacobsen G,Olson AL,Afifi A

更新日期：1990-04-01 00:00:00

abstract：:Open reading frame 89 (ORF89) is one of the three genes that are believed to be involved in the latent infection of white spot syndrome virus (WSSV). Here, we report the structure and functional characterization of ORF89. cDNA sequencing, 5' RLM-RACE, and 3' RLM-RACE showed that ORF89 gene is transcribed into an unspl...

journal_title：Virology

authors： Hossain MS,Khadijah S,Kwang J

更新日期：2004-07-20 00:00:00

abstract：:Three proteins, reacting specifically with sera raised against synthetic peptides identical to C-terminal amino acid sequences in alfalfa mosaic virus (AIMV) proteins P1, P2, and P3 translated in vitro from the AIMV RNAs 1, 2, and 3, respectively, were for the first time observed in tobacco and cowpea protoplasts. Par...

journal_title：Virology

authors： van Pelt-Heerschap H,Verbeek H,Huisman MJ,Sue Loesch-Fries L,Van Vloten-Doting L

更新日期：1987-11-01 00:00:00

abstract：:Six deletion mutations and an insertion were generated in the env gene of cloned copies of Moloney murine leukemia virus DNA. All seven mutants were replication-defective as tested by transformation of NIH/3T3 cells. The mutant DNAs were introduced into NIH/3T3 cells to generate stable producer lines; all released vir...

journal_title：Virology

authors： Granowitz C,Colicelli J,Goff SP

更新日期：1991-08-01 00:00:00

abstract：:An insertion mutation in the pol gene of Moloney murine leukemia virus (M-MuLV) was found to render the virus temperature-sensitive for replication. A provirus containing a 12-bp insertion at the boundary between the reverse transcriptase (RT) and integrase (IN) domains induced the formation of mutant virions containi...

journal_title：Virology

authors： Tanese N,Roth M,Epstein H,Goff SP

更新日期：1989-06-01 00:00:00

abstract：:The bipartite genomic RNAs of red clover necrotic mosaic virus (RCNMV) lack a 5' cap and a 3' poly(A) tail. RNA1 encodes viral replication proteins, and RNA2 encodes a movement protein (MP). These proteins are translated in a cap-independent manner. We previously identified two cis-acting RNA elements that cooperative...

journal_title：Virology

authors： Tajima Y,Iwakawa HO,Hyodo K,Kaido M,Mise K,Okuno T

更新日期：2017-09-01 00:00:00

abstract：:HIV-1 Vpu antagonizes the block to particle release mediated by CD317 (BST-2/HM1.24/Tetherin) via incompletely understood mechanisms. Vpu and CD317 partially reside in cholesterol-rich lipid rafts where HIV-1 budding preferentially occurs. Here we find that lipid raft association of ectopically expressed or endogenous...

journal_title：Virology

authors： Fritz JV,Tibroni N,Keppler OT,Fackler OT

更新日期：2012-03-01 00:00:00

abstract：:We report the isolation and characterization of HEp-2c cell clones obtained after two successive persistent poliovirus (PV) infections. Once cured, some of the cell clones displayed selective permissivity toward the wild-type Mahoney strain and partial resistance to particular mutants of this strain, including the Sab...

journal_title：Virology

authors： Calvez V,Pelletier I,Couderc T,Pavio-Guédo N,Blondel B,Colbère-Garapin F

更新日期：1995-10-01 00:00:00

abstract：:Herpes simplex viruses encode several glycoproteins dispensable for infection and replication in cell culture. Evidence is presented that there exist at least two pathways for viral attachment to cells, i.e., one mediated by the dispensable glycoprotein C (gC) and one independent of that glycoprotein. Thus, whereas th...

journal_title：Virology

authors： Campadelli-Fiume G,Stirpe D,Boscaro A,Avitabile E,Foá-Tomasi L,Barker D,Roizman B

更新日期：1990-09-01 00:00:00

abstract：:HK97 is a temperate dsDNA bacteriophage of Escherichia coli that is structurally similar to phage lambda, with an icosahedral head of triangulation (7) number 7. Although the capsids of several large dsDNA phages have been studied extensively using a variety of biophysical approaches, no high-resolution structure is a...

journal_title：Virology

authors： Wikoff WR,Duda RL,Hendrix RW,Johnson JE

更新日期：1998-03-30 00:00:00

abstract：:Measles virus (MV) causes various responses including the induction of immune responses, transient immunosuppression and establishment of long-lasting immunity. To obtain a comprehensive view of the effects of MV infection on target cells, DNA microarray analyses of two different cell-types were performed. An epitheli...

journal_title：Virology

authors： Sato H,Honma R,Yoneda M,Miura R,Tsukiyama-Kohara K,Ikeda F,Seki T,Watanabe S,Kai C

更新日期：2008-06-05 00:00:00

abstract：:The glycoprotein E (gE) of varicella zoster virus (VZV), encoded by ORF68, is the most abundant viral glycoprotein. In the current experiments, we demonstrated that ORF68 deletion was incompatible with recovery of infectious virus from VZV cosmids. Replacing ORF68 at a nonnative AvrII site in the genome restored infec...

journal_title：Virology

authors： Mo C,Lee J,Sommer M,Grose C,Arvin AM

更新日期：2002-12-20 00:00:00

abstract：:Viral vectors were constructed from infectious cDNA clones of each of the three tobraviruses, tobacco rattle virus (TRV), pea early-browning virus (PEBV), and pepper ringspot virus (PepRSV). RNA2 of each of the three viruses was modified to carry an additional coat protein subgenomic promoter and was used to express g...

journal_title：Virology

authors： MacFarlane SA,Popovich AH

更新日期：2000-02-01 00:00:00

abstract：:Nucleos(t)ide analogues that inhibit hepatitis B virus (HBV) DNA replication are typically used as monotherapy for chronically infected patients. Treatment with a nucleos(t)ide analogue eliminates most HBV DNA replication intermediates and produces a gradual decline in levels of covalently closed circular DNA (cccDNA)...

journal_title：Virology

authors： Reaiche-Miller GY,Thorpe M,Low HC,Qiao Q,Scougall CA,Mason WS,Litwin S,Jilbert AR

更新日期：2013-11-01 00:00:00

abstract：:The guinea pig cytomegalovirus (GPCMV) co-linear gene and potential functional homolog of HCMV UL84 (GP84) was investigated. The GP84 gene had delayed early transcription kinetics and transient expression studies of GP84 protein (pGP84) demonstrated that it targeted the nucleus and co-localized with the viral DNA poly...

journal_title：Virology

authors： McGregor A,Choi KY,Schleiss MR

更新日期：2011-02-05 00:00:00

abstract：:Infections of nonpermissive ( sup0 ) Escherichia coli by T1 phage with amber mutations in either gene 3.5 or gene 4 exhibit a variety of defective phenotypes, including premature arrest of T1 DNA synthesis, failure to make concatemeric DNA, formation of an abnormal DNA replication intermediate, failure to package phag...

journal_title：Virology

authors： Pugh JC,Ritchie DA

更新日期：1984-05-01 00:00:00

abstract：:Natural antibodies to gp41 inhibit HIV-1 replication through the recognition of two different regions, corresponding to the leucine zipper motif in the HR1 alpha-helix and to another motif within HR2 region, hosting 2F5 and 4E10 epitope. This study aimed at reproducing such protective responses through VLP vaccination...

journal_title：Virology

authors： Pastori C,Tudor D,Diomede L,Drillet AS,Jegerlehner A,Röhn TA,Bomsel M,Lopalco L

更新日期：2012-09-15 00:00:00

abstract：:Urine and breast milk represent the main routes of human cytomegalovirus (HCMV) transmission but the contribution of renal and mammary epithelial cells to viral excretion remains unclear. We observed that kidney and mammary epithelial cells were permissive to HCMV infection and expressed immediate early, early and lat...

journal_title：Virology

authors： Twite N,Andrei G,Kummert C,Donner C,Perez-Morga D,De Vos R,Snoeck R,Marchant A

更新日期：2014-07-01 00:00:00

abstract：:Enveloped virus entry occurs when viral and cellular membranes fuse releasing particle contents into the target cell. Human immunodeficiency virus (HIV) entry occurs by cell-free virus or virus transferred between infected and uninfected cells through structures called virological synapses. We developed a high-through...

journal_title：Virology

authors： Esposito AM,Cheung P,Swartz TH,Li H,Tsibane T,Durham ND,Basler CF,Felsenfeld DP,Chen BK

更新日期：2016-03-01 00:00:00

abstract：:RNA interference (RNAi) is the process by which double-stranded RNA (dsRNA) directs sequence-specific degradation of messenger RNA in animal and plant cells. In mammalian cells, RNAi can be triggered by 21-23 nucleotide duplexes of small interfering RNA (siRNA). Strategies to inhibit RNA virus multiplication based on ...

journal_title：Virology

authors： Liu M,Chen W,Ni Z,Yan W,Fei L,Jiao Y,Zhang J,Du Q,Wei X,Chen J,Liu Y,Zheng Z

更新日期：2005-05-25 00:00:00

abstract：:We demonstrate that the AL2 gene of Cabbage leaf curl virus (CaLCuV) activates the CP promoter in mesophyll and acts to derepress the promoter in vascular tissue, similar to that observed for Tomato golden mosaic virus (TGMV). Binding studies indicate that sequences mediating repression and activation of the TGMV and ...

journal_title：Virology

authors： Lacatus G,Sunter G

更新日期：2008-06-20 00:00:00

abstract：:A cytopathic infectious agent was isolated from the kidneys of an apparently healthy tree shrew (Tupaia belangeri) that had been captured in the area around Bangkok. The infectivity was propagated in Tupaia fibroblast and kidney cell cultures. Paramyxovirus-like pleomorphic enveloped particles and helical nucleocapsid...

journal_title：Virology

authors： Tidona CA,Kurz HW,Gelderblom HR,Darai G

更新日期：1999-06-05 00:00:00

abstract：:The spike glycoprotein (G protein) of rabies virus (CVS strain) expressed in HeLa cells from cloned cDNA mediated membrane fusion after exposure to pHs of 6.1 or below. Chemical crosslinking showed that the rabies G protein, like the vesicular stomatitis virus (VSV) G protein, could be crosslinked to dimers and trimer...

journal_title：Virology

authors： Whitt MA,Buonocore L,Prehaud C,Rose JK

更新日期：1991-12-01 00:00:00

abstract：:Extensive cell killing and cytopathology were observed within 24 hr after exposure of a clonal cell line of human T-4 lymphocytes (RH9) to culture supernatants containing human immunodeficiency virus (HIV). Ultraviolet-irradiated HIV-containing culture fluids were also capable of killing RH9 cells and of inducing spec...

journal_title：Virology

authors： Rasheed S,Gottlieb AA,Garry RF

更新日期：1986-10-30 00:00:00

abstract：:Avian myelocytomatosis virus MC29 inoculated into the wing web of 1-day-old Brown Leghorn chickens causes a high incidence of tumors, predominantly endotheliomas that are apparently induced directly by the action of the viral myc gene. Mutants of MC29, in which portions of the v-myc gene have been deleted and which ha...

journal_title：Virology

authors： Enrietto PJ,Hayman MJ,Ramsay GM,Wyke JA,Payne LN

更新日期：1983-01-15 00:00:00

abstract：:We examined the molecular epidemiology and evolution of Ross River (RR) virus in Australia and the Pacific Islands. Nucleotide sequences of the E2 and E3 genes of five RR virus strains revealed remarkable conservation between 1959 and 1989 with a maximum divergence of only 3.3%. Sequence data from a 505-base pair frag...

journal_title：Virology

authors： Sammels LM,Coelen RJ,Lindsay MD,Mackenzie JS

更新日期：1995-09-10 00:00:00

abstract：:Sixteen monoclonal antibodies directed to the hemagglutinin-neuraminidase (HN) protein of a 1957 isolate of parainfluenza type 3 virus (PIV3) were produced and used to examine antigenic variation in clinical strains. Analysis of hemagglutination-inhibition reactivity patterns of antigenic variants selected in vitro in...

journal_title：Virology

authors： van Wyke Coelingh KL,Winter C,Murphy BR

更新日期：1985-06-01 00:00:00

abstract：:H4N8 subtype avian influenza viruses were isolated from shorebirds in eastern Hokkaido. All the isolates shared >99.7% nucleotide homology, and all the viral genes except for PB1 were highly related to those of A/red-necked stint/Australia/1/04. Thus, the isolates were regarded as PB1 reassortants. The most similar PB...

journal_title：Virology

authors： Bui VN,Ogawa H,Xininigen,Karibe K,Matsuo K,Awad SS,Minoungou GL,Yoden S,Haneda H,Ngo LH,Tamaki S,Yamamoto Y,Nakamura K,Saito K,Watanabe Y,Runstadler J,Huettmann F,Happ GM,Imai K

更新日期：2012-02-05 00:00:00