Abstract:
:Information about the basic biological properties of human rhinovirus-C (HRV-C) viruses is lacking due to difficulties with culturing these viruses. Our objective was to develop a cell culture system to grow HRV-C. Epithelial cells from human sinuses (HSEC) were differentiated at air-liquid interface (ALI). Differentiated cultures supported 1-2 logs growth of HRV-C15 as detected by quantitative RT-PCR. Two distinguishing features of HRVs are acid lability and optimal growth at 33-34 °C. We used this system to show that HRV-C15 is neutralized by low pH (4.5). In contrast to most HRV types, replication of HRV-C15 and HRV-C41 was similar at 34 and 37°C. The HSEC ALI provides a useful tool for quantitative studies of HRV-C replication. The ability of HRV-C to grow equally well at 34°C and 37°C may contribute to the propensity for HRV-C to cause lower airway illnesses in infants and children with asthma.
journal_name
Virologyjournal_title
Virologyauthors
Ashraf S,Brockman-Schneider R,Bochkov YA,Pasic TR,Gern JEdoi
10.1016/j.virol.2012.11.002subject
Has Abstractpub_date
2013-02-05 00:00:00pages
143-9issue
1eissn
0042-6822issn
1096-0341pii
S0042-6822(12)00549-1journal_volume
436pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::Cauliflower mosaic virus (CaMV) strains CM1841 and W260 produced markedly different symptoms when inoculated onto turnips (Brassica campestris L. 'Just Right'). The CM1841 strain induced a mild degree of stunting of infected plants while strain W260 caused moderate to severe stunting. Although CM1841 was significantly...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90898-l
更新日期:1991-04-01 00:00:00
abstract::Human schlafen11 is a novel restriction factor for HIV-1 based on bias regarding relative synonymous codon usage (RSCU). Here, we report the cloning of equine schlafen11 (eSLFN11) and the characteristics of its role in restricting the production of equine infectious anemia virus (EIAV), a retrovirus similar to HIV-1. ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2016.04.024
更新日期:2016-08-01 00:00:00
abstract::We measured the effects of non-nucleoside reverse transcriptase (RT) inhibitor-resistant mutations K101E+G190S, on replication fitness and EFV-resistance of HIV(NL4-3). K101E+G190S reduced fitness in the absence of EFV and increased EFV resistance, compared to either single mutant. Unexpectedly, K101E+G190S also repli...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.03.018
更新日期:2010-07-05 00:00:00
abstract::Bluetongue virus (BTV) VP1 protein, a component of the viral RNA-directed RNA polymerase, but not the VP4 or VP6 proteins, was specifically incorporated into baculovirus expressed BTV core-like particles (composed of VP3 and VP7) and BTV virus-like particles (composed of VP2, VP3, VP5, and VP7). The VP1 protein has be...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90094-r
更新日期:1991-02-01 00:00:00
abstract::To clarify the causal relationship between human papillomavirus (HPV) and cervical intraepithelial neoplasia (CIN), we analyzed 386 unfixed biopsy specimens by blot hybridization at Tm -40 degrees C, targeting 38 skin and 42 genital HPVs. By the recognition of PstI, BanI, and MspI cleavage patterns, single genital, bu...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2001.0865
更新日期:2001-04-25 00:00:00
abstract::The pathogenesis of Venezuelan equine encephalitis virus (VEE) was examined in the mouse model using V3000, a virus derived from a molecular clone of the Trinidad donkey strain of VEE. These results were compared in parallel experiments with avirulent mutants of VEE derived by site-directed mutagenesis of the clone. A...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.1022
更新日期:1995-02-01 00:00:00
abstract::Long-term persistent infections were established with the flavivirus, West Nile virus (WNV), strain E101, in embryofibroblast cultures derived from susceptible C3H/HE and congenic-resistant C3H/RV mice. Cultures were initially maintained by weekly subculture at 37 degrees, but at passage 6 sister cultures were shifted...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90454-4
更新日期:1985-01-15 00:00:00
abstract::Previous studies indicated that hepatitis C virus core protein influences cellular apoptosis. However, the precise mechanisms of the effects are not fully understood. Therefore, in this study, we examined the mechanisms of the effects on cell apoptosis by core protein, using transiently transfected and magnetically co...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2002.1371
更新日期:2002-04-25 00:00:00
abstract::Measles virus (MV) inhibits lymphocyte function in patients, as well as in cells infected in vitro. The proliferation of phytohemagglutinin-stimulated T lymphocytes is suppressed by in vitro MV infection, as shown by the diminished incorporation of [3H]thymidine into DNA and the reduced frequency of cells in the S pha...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90316-h
更新日期:1992-03-01 00:00:00
abstract::Coronavirus (CoV) envelope (E) protein ion channel activity was determined in channels formed in planar lipid bilayers by peptides representing either the transmembrane domain of severe acute respiratory syndrome CoV (SARS-CoV) E protein, or the full-length E protein. Both of them formed a voltage independent ion cond...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2012.07.005
更新日期:2012-10-25 00:00:00
abstract::Seven structural component proteins of a paramyxovirus simian virus 41 (SV41) were identified with the aid of monoclonal antibodies prepared against SV41 and human parainfluenza type 2 virus (PIV2). The nucleoprotein is antigenically very close to that of PIV2, while it is comparatively far from that of simian virus 5...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90141-d
更新日期:1990-12-01 00:00:00
abstract::BS-1, a stroma cell line derived from normal human bone marrow, can support the growth of murine erythroid (BFU-E), granulocyte-macrophage (CFU-GM), and megakaryocyte (CFU-Meg) progenitor cells in a short-term in vitro coculture system. Exposure of BS-1 cell to the human immunodeficiency virus (HIV) prior to coculture...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1159
更新日期:1993-03-01 00:00:00
abstract::We reported earlier that variable expression of the Epstein-Barr virus (EBV) encoded membrane protein LMP 1 in nasopharyngeal carcinoma and the host-cell-phenotype-dependent activity of the BCR2 promoter (one of the possible initiator sites for transcripts of Epstein-Barr nuclear antigens) in Burkitt's lymphoma (BL) l...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1229
更新日期:1994-05-01 00:00:00
abstract::Single intradermal or intramuscular inoculations of GM-CSF DNA with the DNA prime for a simian-human immunodeficiency virus (SHIV)-89.6 vaccine, which consists of DNA priming followed by modified vaccinia Ankara (MVA) boosting, increased protection of both the blood and intestines against the acute phase of an intrare...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2007.07.017
更新日期:2007-12-05 00:00:00
abstract::Influenza viruses grown in embryonated chicken eggs frequently possess antigenically distinguishable hemagglutinin (HA) compared to virus from the same source grown in mammalian cell culture. To further investigate the extent of variation among viruses from an individual, viruses were isolated from throat washes colle...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90588-0
更新日期:1988-08-01 00:00:00
abstract::Thirteen viable insertion mutants of vaccinia virus have been constructed. These mutants, containing coding sequences of the herpes simplex virus thymidine kinase (HSV-TK) gene, were generated by marker transfer via in vivo recombination. The mutants were identified using a replica filter plating technique by in situ ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90132-7
更新日期:1986-07-30 00:00:00
abstract::Extra MMTV proviruses in T-cell leukemias of GR and C57/BL10 mice contain alterations in their long terminal repeat (LTR) sequence. The four different leukemias studied contain different deletions, but common hallmarks were observed around the recombination sites. At the 5' end of the deletions we observed a common no...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90431-9
更新日期:1986-10-15 00:00:00
abstract::Piglets infected in utero with porcine reproductive and respiratory syndrome virus (PRRSV) are born severely immunocompromised. In this article we more closely examine the effects of in utero PRRSV infection on circulating and thymic T cell populations. Numbers of CD4+, CD8+, and dual-positive lymphocytes were quantit...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2002.1650
更新日期:2002-10-25 00:00:00
abstract::Virions of southern bean mosaic virus (SBMV) show two distinct sensitivity patterns upon heating. Infectivity loss and capsid denaturation occur concurrently if virions are exposed at 50-55 degrees in 0.1 M sodium phosphate buffer, pH 7.5, or 0.1 M glycine-phosphate buffer, pH 9.0. Contrastingly, virions are inactivat...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(81)90437-2
更新日期:1981-01-30 00:00:00
abstract::Antigenic variation among influenza B viruses is different from that of influenza A in several ways. Antigenic shift has not been observed, distinct antigenic variants of influenza B cocirculate, and antigenically similar viruses have been isolated many years apart. To study the mechanism of antigenic drift in influen...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90384-2
更新日期:1984-12-01 00:00:00
abstract::Spontaneous proteolysis of influenza virus M1 protein during crystallisation has defined an N-terminal domain of amino acids 1--164. Full-length M1, the N-terminal domain, and the C-terminal part of M1 (residues 165--252) were produced in Escherichia coli. In vitro tests showed that only full-length M1 and its N-termi...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2000.0804
更新日期:2001-03-01 00:00:00
abstract::The circular single-stranded viral DNA (ssDNA) of filamentous phage is oriented in the virus with a hairpin forming sequence called mos at the leading end of the virion-the end that emerges from the cell first. In the experiments that originally defined mos it was also found to enhance virion production 100-fold, thou...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90066-9
更新日期:1988-11-01 00:00:00
abstract::Recombinant baculoviruses containing the coding sequences of the viral structural proteins, i.e., the capsid (C) protein, the precursor to premembrane (preM) protein, and the envelope (E) protein, as well as a nonstructural protein, NS1, of Japanese encephalitis virus (JEV) were constructed. Infection of Spodoptera fr...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90580-1
更新日期:1989-12-01 00:00:00
abstract::Retroperitoneal fibromatosis herpesvirus (RFHV), the macaque homolog of the human rhadinovirus, Kaposi's sarcoma-associated herpesvirus (KSHV), was first identified in retroperitoneal fibromatosis (RF) tumor lesions of macaques with simian AIDS. We cloned and sequenced the ORF73 latency-associated nuclear antigen (LAN...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.06.022
更新日期:2006-10-10 00:00:00
abstract::We have developed a sensitive assay for DNA replication in mammalian cells that enables us to detect replicated DNA fragments of human cytomegalovirus. A 1/100 portion of DNA extracted from 1000 microinjected cells was subjected to polymerase chain reaction after digestion with appropriate restriction enzymes to diffe...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1038
更新日期:1993-01-01 00:00:00
abstract::Previously we have reported that the essential 3a movement gene of icosahedral cowpea chlorotic mottle virus (CCMV) can be functionally replaced by the 30-kDa movement gene of rod-shaped sunn-hemp mosaic virus (SHMV). Because plant RNA viruses differ in requiring or not requiring coat protein for systemic infection, w...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8544
更新日期:1997-05-26 00:00:00
abstract::Two temperature-sensitive mutants of herpes simplex virus type 1 in complementation group 1-1 were analyzed to determine if the major DNA-binding protein they produced was thermolabile. Cells infected with these mutants were analyzed for deoxyribonucleoprotein complexes containing the DNA-binding protein. These comple...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90220-4
更新日期:1984-09-01 00:00:00
abstract::Porcine epidemic diarrhea virus (PEDV) is a causative agent of porcine epidemic diarrhea (PED). PED, characterized by acute diarrhea, vomiting, dehydration, has caused serious economic losses in pig industry worldwide. Here, we demonstrate that activation of a disintergrin and metalloprotease 17 (ADAM17) induced the d...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2018.02.001
更新日期:2018-04-01 00:00:00
abstract::The Rice stripe virus (RSV) pc4 has been determined as the viral movement protein (MP). In this study, the pc4 gene was cloned into a movement-deficient Tobacco mosaic virus (TMV). The resulting hybrid TMV-pc4, in addition to spreading cell to cell in Nicotiana tabacum, moved systemically and induced foliar necrosis i...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2012.01.007
更新日期:2012-04-10 00:00:00
abstract::The shuttle vector plasmid pZ189 was used as a target for mutagenesis in COS-1 cells. Complex mutations were analyzed in 5 plasmids that were recovered from noninfected cells and 15 plasmids that were recovered from cells that were infected with herpes simplex virus type-1 (HSV-1). Complex mutations in noninfected cel...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90895-i
更新日期:1991-04-01 00:00:00