Abstract:
:Spontaneous proteolysis of influenza virus M1 protein during crystallisation has defined an N-terminal domain of amino acids 1--164. Full-length M1, the N-terminal domain, and the C-terminal part of M1 (residues 165--252) were produced in Escherichia coli. In vitro tests showed that only full-length M1 and its N-terminal domain bind to negatively charged liposomes and that only full-length M1 and its C-terminal part bind to RNP. However, only full-length M1 had transcription inhibition activity. Several independent experimental approaches indicate that in vitro transcription inhibition occurs through polymerisation/aggregation of M1 onto RNP, or of M1 onto M1 already bound to RNP, rather than by binding to a specific active site on the nucleoprotein or the polymerase. The structure/function of influenza virus M1 will be compared with that of the Ebola virus matrix protein, VP40.
journal_name
Virologyjournal_title
Virologyauthors
Baudin F,Petit I,Weissenhorn W,Ruigrok RWdoi
10.1006/viro.2000.0804subject
Has Abstractpub_date
2001-03-01 00:00:00pages
102-8issue
1eissn
0042-6822issn
1096-0341pii
S0042-6822(00)90804-3journal_volume
281pub_type
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