Abstract:
:We show that the product of the polarity suppression (psu) gene from bacteriophage P4 associates with P4 capsids. This association can occur when Psu is (i) provided in vivo from the P4 genome or from a plasmid or (ii) provided in vitro by mixing viable phage particles with Psu protein. Psu is unable to associate with the larger capsid of P4's helper phage P2. Discrimination of the P4 and P2 capsids by Psu appears to be independent of the presence of the P4 genome in the capsid, since P2 size capsids filled with P4 DNA cannot accommodate Psu association. P4 psu particles devoid of Psu are less stable than P4 particles carrying Psu. These results indicate that, in addition to its antitermination activity at Rho-dependent terminators, Psu is also a decoration protein that stabilizes the P4 capsids.
journal_name
Virologyjournal_title
Virologyauthors
Isaksen ML,Rishovd ST,Calendar R,Lindqvist BHdoi
10.1016/0042-6822(92)90538-zsubject
Has Abstractpub_date
1992-06-01 00:00:00pages
831-9issue
2eissn
0042-6822issn
1096-0341pii
0042-6822(92)90538-Zjournal_volume
188pub_type
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