当前位置： SCI文献检索 > VIROLOGY期刊下所有文献 > Molecular cloning of Lassa virus RNA: nucleotide sequence and expression of the nucleocapsid protein gene.

Molecular cloning of Lassa virus RNA: nucleotide sequence and expression of the nucleocapsid protein gene.

Abstract：

:Lassa virus RNA isolated from purified virus particles was polyadenylated and reverse transcripts were cloned into the PstI site of plasmid pUC9. Clones containing sequences of the smaller (S) segment of the Lassa virus genome were identified by hybridization with purified S RNA. They were characterized by their ability to hybridize with fragments of 3'-labeled Lassa virus S RNA and with each other, and by restriction mapping. The largest insert was 1830 bp long and began with the 3'-terminal 19-base sequence characteristic of all arenavirus S RNAs so far analyzed. The virus complementary strand contained a single large open reading frame, beginning at the ATG nearest its 5' end (nucleotides 103-5) and terminating with a TGA triplet at position 1813-15, that encodes a protein of 570 amino acids. A recombinant was constructed which expressed the gene as a fusion protein in Escherichia coli. The product was a 60-kDa polypeptide which reacted with monoclonal antibodies specific for the nucleocapsid protein. Comparison of the predicted amino acid sequence with the corresponding sequences deduced from the nucleotide sequences of the other arenavirus S RNAs, lymphocytic choriomeningitis (LCM) and Pichinde, reveals a considerable degree of similarity between Old and New World arenaviruses.

journal_name

Virology

journal_title

Virology

authors

Clegg JC,Oram JD

doi

10.1016/0042-6822(85)90278-8

subject

Has Abstract

pub_date

1985-07-30 00:00:00

pages

363-72

issue

2

eissn

0042-6822

issn

1096-0341

journal_volume

144

pub_type

杂志文章

相关文献

VIROLOGY文献大全
  • Anti-gD monoclonal antibodies inhibit cell fusion induced by herpes simplex virus type 1.

    abstract：:Monoclonal antibodies directed against glycoprotein D of herpes simplex virus completely inhibited fusion of Vero cells infected with type 1 virus. In contrast, several monoclonal antibodies directed against other viral glycoproteins, including B, were ineffective or were only minimally inhibitory at the highest conce...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(83)90409-9

    authors： Noble AG,Lee GT,Sprague R,Parish ML,Spear PG

    更新日期：1983-08-01 00:00:00

  • Functional domains in the feline immunodeficiency virus nucleocapsid protein.

    abstract：:Retroviral nucleocapsid (NC) proteins are small Gag-derived products containing one or two zinc finger motifs that mediate genomic RNA packaging into virions. In this study, we addressed the role of the feline immunodeficiency virus (FIV) NC protein in the late stages of virus replication by analyzing the assembly phe...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2004.06.019

    authors： Manrique ML,Rauddi ML,González SA,Affranchino JL

    更新日期：2004-09-15 00:00:00

  • Autographa californica multiple nucleopolyhedrovirus core gene ac92 (p33) is required for efficient budded virus production.

    abstract：:Autographa californica multiple nucleopolyhedrovirus (AcMNPV) ac92 is a core gene encoding a protein associated with occlusion derived virus (ODV), binds human P53 and also has flavin adenine dinucleotide linked sulfhydryl oxidase activity but its role in the virus life cycle is not known. To determine ac92 function a...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2010.09.023

    authors： Nie Y,Fang M,Theilmann DA

    更新日期：2011-01-05 00:00:00

  • BST-2 mediated restriction of simian-human immunodeficiency virus.

    abstract：:Pathogenic simian-human immunodeficiency viruses (SHIV) contain HIV-1 Vpu and SIV Nef, both shown to counteract BST-2 (HM1.24; CD317; tetherin) inhibition of virus release in a species-specific manner. We show that human and pig-tailed BST-2 (ptBST-2) restrict SHIV. We found that sequential "humanization" of the trans...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2010.07.021

    authors： Ruiz A,Lau D,Mitchell RS,Hill MS,Schmitt K,Guatelli JC,Stephens EB

    更新日期：2010-10-25 00:00:00

  • Small amino acid sequence changes within the V2 domain can affect the function of a T-cell line-tropic human immunodeficiency virus type 1 envelope gp120.

    abstract：:Prior studies with recombinant viruses constructed in vitro showed that the V2 domain of envelope gp120, in addition to the required V3 domain, enhances the efficiency of infection of primary macrophages by HIV-1. Present structural studies on the gp120s of these recombinant viruses using three human monoclonal antibo...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1995.1010

    authors： Koito A,Stamatatos L,Cheng-Mayer C

    更新日期：1995-02-01 00:00:00

  • Viral fitness implications of variation within an immunodominant CD8+ T-cell epitope of HIV-1.

    abstract：:Cytotoxic T-lymphocyte (CTL) epitopes within the HIV genome are subject to negative and positive selective pressures, the balance of which influences CTL escape at a given epitope. We investigated whether viral fitness requirements dictate conservation of the HLA-A2 restricted immunodominant epitope SLYNTVATL (SL9). V...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2009.03.003

    authors： Christie NM,Willer DO,Lobritz MA,Chan JK,Arts EJ,Ostrowski MA,Cochrane A,Luscher MA,MacDonald KS

    更新日期：2009-05-25 00:00:00

  • Altered pathogenicity of avian myelocytomatosis (MC29) viruses with mutations in the v-myc gene.

    abstract：:Avian myelocytomatosis virus MC29 inoculated into the wing web of 1-day-old Brown Leghorn chickens causes a high incidence of tumors, predominantly endotheliomas that are apparently induced directly by the action of the viral myc gene. Mutants of MC29, in which portions of the v-myc gene have been deleted and which ha...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(83)90300-8

    authors： Enrietto PJ,Hayman MJ,Ramsay GM,Wyke JA,Payne LN

    更新日期：1983-01-15 00:00:00

  • Characterization of infectious and defective cloned avian hepadnavirus genomes.

    abstract：:The infectivity in vivo, replication competence in vitro, and expression of viral genes of several molecularly cloned duck hepatitis B virus (DHBV) genomes were investigated. In addition, replication competence, core protein expression, and secretion of viral proteins were investigated for a grey heron hepatitis B vir...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(91)90782-7

    authors： Wildner G,Fernholz D,Sprengel R,Schneider R,Will H

    更新日期：1991-11-01 00:00:00

  • Packaging of AeDNV-GFP transducing virus by expression of densovirus structural proteins from a sindbis virus expression system.

    abstract：:Genetic recombination resulting in the production of wild-type infectious virus is an obstacle in the current system for producing densovirus transducing particles. In order to eliminate this problem, a double subgenomic Sindbis virus (TE/3'2J/VP) was engineered that expresses the structural proteins (VPs) of Aedes de...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1999.9622

    authors： Allen-Miura TM,Afanasiev BN,Olson KE,Beaty BJ,Carlson JO

    更新日期：1999-04-25 00:00:00

  • Microevolution of tick-borne encephalitis virus in course of host alternation.

    abstract：:Two tick-borne encephalitis (TBE) virus variants were studied: mouse brain-adapted strain EK-328 and its derivate adapted to Hyalomma marginatum ticks. The tick-adapted virus exhibited small-plaque phenotype and slower replication in PEK cells, higher yield in ticks, decreased neuroinvasiveness in mice, increased bind...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2006.12.013

    authors： Romanova LIu,Gmyl AP,Dzhivanian TI,Bakhmutov DV,Lukashev AN,Gmyl LV,Rumyantsev AA,Burenkova LA,Lashkevich VA,Karganova GG

    更新日期：2007-05-25 00:00:00

  • Vaccinia virus gene D12L encodes the small subunit of the viral mRNA capping enzyme.

    abstract：:Vaccinia virus gene D12L, which lies between nucleotides 14,350 and 13,487 in the HindIII D fragment, is transcribed at early times in infection and is capable of encoding a protein 287 amino acids in length with a predicted molecular mass of 33,331. A polyclonal antiserum was raised in rabbits to a fusion protein con...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(89)90194-3

    authors： Niles EG,Lee-Chen GJ,Shuman S,Moss B,Broyles SS

    更新日期：1989-10-01 00:00:00

  • The insertion domain of the duck hepatitis B virus core protein plays a role in nucleocapsid assembly.

    abstract：:Synthesis of hepadnaviral DNA is dependent upon both the viral DNA polymerase and the viral core protein, the subunit of the nucleocapsids in which viral DNA synthesis takes place. In a study of natural isolates of duck hepatitis B virus (DHBV), we cloned full-length viral genomes from a puna teal. One of the clones f...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2006.06.004

    authors： Guo H,Aldrich CE,Saputelli J,Xu C,Mason WS

    更新日期：2006-09-30 00:00:00

  • Complete nucleotide sequences of 5'- and 3'-noncoding regions of both genome segments of different strains of infectious bursal disease virus.

    abstract：:The terminal sequences of both genomic dsRNA segments of different strains of infectious bursal disease virus were determined. Analysis of the linear sequences revealed in the 5'-noncoding region of both segments the presence of a 32-nucleotide sequence with motifs conserved between segments and serotypes. The 3'-nonc...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1995.1226

    authors： Mundt E,Müller H

    更新日期：1995-05-10 00:00:00

  • Expression of the vesicular stomatitis virus nucleocapsid protein gene in Escherichia coli: analysis of its biological activity in vitro.

    abstract：:The nucleocapsid (N) protein genes of vesicular stomatitis virus, serotypes New Jersey and Indiana, were expressed in Escherichia coli BL21(DE3) transformed by the recombinant expression vector pET-3a containing N genes under the control of the T7 RNA polymerase promoter. Following induction with isopropylthiogalactop...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1993.1130

    authors： Das T,Banerjee AK

    更新日期：1993-03-01 00:00:00

  • Epitopes of the G1 glycoprotein of La Crosse virus form overlapping clusters within a single antigenic site.

    abstract：:Antigenic sites on the G1 glycoprotein of La Crosse bunyavirus were defined by constructing a panel of neutralizing and nonneutralizing monoclonal antibodies (F. Gonzalez-Scarano, R. E. Shope, C. H. Calisher, and N. Nathanson (1982), Virology 120, 42-53). To analyze the relationship between the individual epitopes del...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(85)90283-1

    authors： Najjar JA,Gentsch JR,Nathanson N,Gonzalez-Scarano F

    更新日期：1985-07-30 00:00:00

  • The Epstein-Barr virus BRRF2 gene product is involved in viral progeny production.

    abstract：:The Epstein-Barr virus (EBV) predominantly establishes a latent infection in B lymphocytes, and occasionally switches from the latent state to the lytic cycle. In this report, we identified and examined the role of a lytic gene, BRRF2. We first prepared an antibody against BRRF2 and identified the gene product as a vi...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2015.05.010

    authors： Watanabe T,Tsuruoka M,Narita Y,Katsuya R,Goshima F,Kimura H,Murata T

    更新日期：2015-10-01 00:00:00

  • Establishment and characterization of plasmid-driven minigenome rescue systems for Nipah virus: RNA polymerase I- and T7-catalyzed generation of functional paramyxoviral RNA.

    abstract：:In this study we report the development and optimization of two minigenome rescue systems for Nipah virus, a member of the Paramyxoviridae family. One is mediated by the T7 RNA polymerase supplied either by a constitutively expressing cell line or by transfection of expression plasmids and is thus independent from inf...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2007.08.008

    authors： Freiberg A,Dolores LK,Enterlein S,Flick R

    更新日期：2008-01-05 00:00:00

  • Rice tungro bacilliform virus open reading frame 3 encodes a single 37-kDa coat protein.

    abstract：:Rice tungro bacilliform virus (RTBV) is a plant pararetrovirus and a member of the Caulimoviridae family and closely related to viruses in the Badnavirus genus. The coat protein of RTBV is part of the large polyprotein encoded by open reading frame 3 (ORF3). ORF3 of an RTBV isolate from Malaysia was sequenced (accessi...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1998.9519

    authors： Marmey P,Bothner B,Jacquot E,de Kochko A,Ong CA,Yot P,Siuzdak G,Beachy RN,Fauquet CM

    更新日期：1999-01-20 00:00:00

  • Construction of chimeric arteriviruses reveals that the ectodomain of the major glycoprotein is not the main determinant of equine arteritis virus tropism in cell culture.

    abstract：:The recent development of arterivirus full-length cDNA clones makes possible the construction of chimeric arteriviruses for fundamental and applied studies. Using an equine arteritis virus (EAV) infectious cDNA clone, we have engineered chimeras in which the ectodomains of the two major envelope proteins, the glycopro...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.2001.1074

    authors： Dobbe JC,van der Meer Y,Spaan WJ,Snijder EJ

    更新日期：2001-09-30 00:00:00

  • Interferon-alpha-induced gene expression: evidence for a selective effect of ouabain on activation of the ISGF3 transcription complex.

    abstract：:Binding of interferons (IFNs) to their cell surface receptors stimulates rapid translocation of cytoplasmic proteins to the nucleus and the expression of a variety of cellular genes within minutes. Translocated proteins subsequently bind to the interferon-stimulated response element (ISRE) located in the promoters of ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(92)91207-b

    authors： Nakagawa Y,Petricoin EF 3rd,Akai H,Grimley PM,Rupp B,Larner AC

    更新日期：1992-09-01 00:00:00

  • Human papillomavirus type 16 E7 oncoprotein engages but does not abrogate the mitotic spindle assembly checkpoint.

    abstract：:The mitotic spindle assembly checkpoint (SAC) ensures faithful chromosome segregation during mitosis by censoring kinetochore-microtubule interactions. It is frequently rendered dysfunctional during carcinogenesis causing chromosome missegregation and genomic instability. There are conflicting reports whether the HPV1...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2012.06.006

    authors： Yu Y,Munger K

    更新日期：2012-10-10 00:00:00

  • In vivo infection of IgG-containing cells by Jembrana disease virus during acute infection.

    abstract：:Jembrana disease virus (JDV) is an unusual bovine lentivirus which causes a non-follicular proliferation of lymphocytes, a transient immunosuppression and a delayed humoral response in infected Bali cattle in Indonesia. A double-immunofluorescent labeling method was developed to identify the subset of mononuclear cell...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2009.07.027

    authors： Desport M,Tenaya IW,McLachlan A,McNab TJ,Rachmat J,Hartaningsih N,Wilcox GE

    更新日期：2009-10-25 00:00:00

  • Herpes simplex virus immediate early gene expression in the absence of transinduction by Vmw65 varies during the cell cycle.

    abstract：:The requirement for the herpes simplex virus type 1 (HSV-1) protein Vmw65 (VP16) for activation of immediate early (IE) gene expression was examined in synchronized HeLa cells. Analyses of IE RNA levels were conducted during infection with a viral Vmw65 mutant, in1814. The results revealed an increased requirement for...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(92)90695-l

    authors： Daksis JI,Preston CM

    更新日期：1992-07-01 00:00:00

  • A fused gene of nucleoprotein (NP) and herpes simplex virus genes (VP22) induces highly protective immunity against different subtypes of influenza virus.

    abstract：:We evaluated the immunogenicity and protective activity of plasmid DNA vaccines encoding the influenza virus NP gene (pNP) alone or in combination with the herpes simplex virus type 1 protein 22 gene (pVP22). Optimal immune responses were observed in BALB/c mice immunized with the combination of pVP22 plus pNP, as ass...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2006.04.015

    authors： Saha S,Yoshida S,Ohba K,Matsui K,Matsuda T,Takeshita F,Umeda K,Tamura Y,Okuda K,Klinman D,Xin KQ,Okuda K

    更新日期：2006-10-10 00:00:00

  • Induction of herpes simplex virus gB-specific cytotoxic T lymphocytes in TAP1-deficient mice by genetic immunization but not HSV infection.

    abstract：:Loading of most endogenous peptides on major histocompatibility complex class I molecules is conditional on their transport into the endoplasmic reticulum (ER) by the peptide transporter TAP. We describe an HSV-2/1 cross-reactive cytotoxic T-cell (CTL) epitope that is processed in a TAP1-independent manner in vivo fol...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.2000.0829

    authors： Paliard X,Doe B,Selby MJ,Hartog K,Lee AY,Burke RL,Walker CM

    更新日期：2001-03-30 00:00:00

  • The roles of the red clover necrotic mosaic virus capsid and cell-to-cell movement proteins in systemic infection.

    abstract：:The red clover necrotic mosaic dianthovirus (RCNMV) genome is split between two single-stranded RNA species termed RNA-1 and RNA-2. RNA-2 is required for infection of whole plants but is dispensable for infection and virion formation in protoplasts. We have used full-length cDNA clones of RNA-1 and -2 from which infec...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1993.1004

    authors： Xiong Z,Kim KH,Giesman-Cookmeyer D,Lommel SA

    更新日期：1993-01-01 00:00:00

  • Identification, expression, and processing of an 87-kDa polypeptide encoded by ORF 1a of the coronavirus infectious bronchitis virus.

    abstract：:Nucleotide sequence analysis has shown previously that the genomic-length mRNA (mRNA1) of the coronavirus infectious bronchitis virus (IBV) contains two large open reading frames (ORFs), 1a and 1b, with the potential to encode polyproteins of approximately 441 and 300 kDa, respectively. We have characterized the speci...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1995.1128

    authors： Liu DX,Tibbles KW,Cavanagh D,Brown TD,Brierley I

    更新日期：1995-04-01 00:00:00

  • A major DNA binding protein encoded by BALF2 open reading frame of Epstein-Barr virus (EBV) forms a complex with other EBV DNA-binding proteins: DNAase, EA-D, and DNA polymerase.

    abstract：:A major 135-kDa DNA binding protein (mDBP) encoded by the BALF2 open reading frame of Epstein-Barr Virus (EBV) is known to be an essential protein for the induction of the lytic cycle. The present investigation was carried out to know whether this protein forms a complex in vivo with other viral DNA binding proteins (...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1997.8891

    authors： Zeng Y,Middeldorp J,Madjar JJ,Ooka T

    更新日期：1997-12-22 00:00:00

  • Segmental configuration and putative origin of the reassortant orbivirus, epizootic hemorrhagic disease virus serotype 6, strain Indiana.

    abstract：:In 2006, an exotic reassortant orbivirus, epizootic hemorrhagic disease virus serotype 6 (EHDV-6) [strain (Indiana)], was first detected in the United States. To characterize the reassortment configuration of this virus and to conclusively determine the parental virus of each RNA segment, the complete genome of EHDV-6...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2011.12.004

    authors： Allison AB,Holmes EC,Potgieter AC,Wright IM,Sailleau C,Breard E,Ruder MG,Stallknecht DE

    更新日期：2012-03-01 00:00:00

  • Nucleotide sequence of the gene encoding infectious laryngotracheitis virus glycoprotein B.

    abstract：:The nucleotide sequence of the infectious laryngotracheitis virus (ILTV) gene encoding the 205K complex glycoprotein (gp205) was determined. The gene is contained within a 3-kb EcoRI restriction fragment mapping at approximately map coordinates 0.23 to 0.25 in the UL region of the ILTV genome and is transcribed from r...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(91)90859-a

    authors： Kongsuwan K,Prideaux CT,Johnson MA,Sheppard M,Fahey KJ

    更新日期：1991-09-01 00:00:00