Abstract:
:Pathogenic simian-human immunodeficiency viruses (SHIV) contain HIV-1 Vpu and SIV Nef, both shown to counteract BST-2 (HM1.24; CD317; tetherin) inhibition of virus release in a species-specific manner. We show that human and pig-tailed BST-2 (ptBST-2) restrict SHIV. We found that sequential "humanization" of the transmembrane domain (TMD) of the pig-tailed BST-2 (ptBST-2) protein resulted in a fluctuation in sensitivity to HIV-1 Vpu. Our results also show that the length of the TMD in human and ptBST-2 proteins is important for BST-2 restriction and susceptibility to Vpu. Taken together, our results emphasize the importance of tertiary structure in BST-2 antagonism and suggests that the HIV-1 Vpu transmembrane domain may have additional functions in vivo unrelated to BST-2 antagonism.
journal_name
Virologyjournal_title
Virologyauthors
Ruiz A,Lau D,Mitchell RS,Hill MS,Schmitt K,Guatelli JC,Stephens EBdoi
10.1016/j.virol.2010.07.021subject
Has Abstractpub_date
2010-10-25 00:00:00pages
312-21issue
2eissn
0042-6822issn
1096-0341pii
S0042-6822(10)00474-5journal_volume
406pub_type
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