Abstract:
:Maize streak virus (MSV) is a leafhopper-transmitted geminivirus containing one molecule of circular single-stranded DNA of about 2.7 kb. We have tested the infectivity of MSV mutants. A deletion of 29 bases in the small intergenic region (SIR) did not affect the infectivity of MSV. Mutants containing insertions of oligonucleotides of up to 32 bases at the AsnI site in SIR were also infectious. However, the infection efficiency of the insertion mutants decreased as the size of the oligonucleotides increased. This reduced virulence may be due to a decreased efficiency of 3'-end formation of the complementary sense mRNA(s). The stability of MSV mutants in infected maize plants was analyzed by polymerase chain reaction (PCR). Some oligonucleotide-insertion mutants were completely stable while others obtained deletions. Infectivity of the mutants, however, did not require deletion formation. Deletion rearrangement was shown to be dependent on the sequence of the inserted oligonucleotide. The AsnI site is the only site known to permit insertion of nonviral DNA without abolishing MSV infectivity. The system described may allow the use of MSV as a delivery vector to introduce at least small segments of DNA into maize.
journal_name
Virologyjournal_title
Virologyauthors
Shen WH,Hohn Bdoi
10.1016/0042-6822(91)91001-wsubject
Has Abstractpub_date
1991-08-01 00:00:00pages
721-30issue
2eissn
0042-6822issn
1096-0341pii
0042-6822(91)91001-Wjournal_volume
183pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::Proviruses were cloned directly from a cat that developed neurological disorders approximately 28 months after inoculation with a molecularly cloned, minimally pathogenic subgroup A feline leukemia virus (FeLV-A). In addition to FeLV-A proviruses that were nearly identical to the inoculated virus, we detected a subgro...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1597
更新日期:1994-11-01 00:00:00
abstract::Cell lines persistently infected with foot-and-mouth disease virus (FMDV) have been established by growth of BHK-21 (c-13) or IBRS-2 (c-26) that survived standard cytolytic infections with FMDV. They maintain cytoplasmic FMDV RNA sequences, as shown by dot blot hybridization tests, using cloned FMDV cDNA as probes. Ce...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90198-9
更新日期:1985-08-01 00:00:00
abstract::Adenovirus 12 (Ad12), but not adenovirus 2 or 5, induces metaphase chromosome fragility at four specific loci in humans: RNU1, RNU2, PSU1, and RN5S. As each of these sites corresponds to a tandemly repeated multigene family encoding a small, abundant structural RNA, we proposed that Ad12 hinders metaphase chromatin co...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9512
更新日期:1999-02-01 00:00:00
abstract::The phosphorylation and transcriptional competence of the free cytoplasmic form and the virion form of NS protein of vesicular stomatitis virus (VSV-Indiana/Mudd-Summers) were compared. NS protein is known to exist in two distinct phosphorylated states, NS1 and NS2, that are resolvable by gel electrophoresis. In vitro...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90452-6
更新日期:1986-10-30 00:00:00
abstract::Inbred mice bearing the FV-1n marker when inoculated at birth with an ecotropic murine leukemia virus (WM 1504-E) obtained from wild mice develop a progressive central nervous system (CNS) disease manifested by hindlimb paralysis and incoordination that begins by 6 to 7 weeks of age. Studies of infected SWR/J mice at ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(83)90326-4
更新日期:1983-07-15 00:00:00
abstract::Infected cell protein (ICP) 27 is an essential herpes simplex virus type 1 (HSV-1) phosphoprotein required for optimal viral DNA and early or late gene synthesis. Three slow-migrating immunoreactive species were detected using multiple anti-ICP27 antibodies following HSV-1 infection of HEp-2 and Vero cells in the pres...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2004.04.011
更新日期:2004-07-01 00:00:00
abstract::Lipid binding properties which are dependent on exposure to acid pH are an important mechanism for the cellular entry pathway for some enveloped viruses and for other macromolecules. Cell-to-cell fusion can be used to demonstrate this function. La Crosse virus, a member of the family Bunyaviridae, fused BHK-21 cells f...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90107-7
更新日期:1984-01-15 00:00:00
abstract::Aside from a common gene organization shared with other picornaviruses, hepatitis A virus (HAV) is characterized by its slow-growth phenotype, the inability to shut off host macromolecular synthesis, and, in general, lack of cytopathic (cp) effects in permissive cell cultures. Nevertheless, several cp HAV strains have...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1999.0070
更新日期:2000-01-05 00:00:00
abstract::The 1.8-kbp M1 double-stranded (ds) RNA from type 1 killer strains of Saccharomyces cerevisiae contains an internal 200-bp adenine- and uracil-rich region. We have previously demonstrated that this region consists primarily of adenine residues on the plus strand of M1 dsRNA and on the full-length, in vitro synthesized...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90380-6
更新日期:1986-07-15 00:00:00
abstract::Hantaan virus, the prototype virus of hemorrhagic fever with renal syndrome, was examined for nucleic acid characteristics which would support its previously proposed inclusion in the virus family Bunyaviridae. Nucleocapsid RNA from Hantaan virions and a control bunyavirus were examined for ribonuclease A (RNase A) se...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(83)90514-7
更新日期:1983-12-01 00:00:00
abstract::The causal agent of rhizomania disease, Beet necrotic yellow vein virus (BNYVV), typically produces asymptomatic root-limited infections in sugar beets (Beta vulgaris) carrying the Rz1-allele. Unfortunately, this dominant resistance has been recently overcome. Multiple cDNA clones of the viral pathogenic determinant p...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.03.008
更新日期:2008-06-20 00:00:00
abstract::The retroviral integrase (IN) is a virus-encoded enzyme that is essential for insertion of viral DNA into the host chromosome. In order to map and define the properties of a minimal functional domain for this unique viral enzyme, a series of N- and C-terminal deletions of both Rous sarcoma virus (RSV) and human immuno...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(95)80060-3
更新日期:1995-01-10 00:00:00
abstract::The orthopoxvirus serpin SPI-3 is N-glycosylated and suppresses fusion between infected cells. Although SPI-3 contains motifs conserved in inhibitory serpins, no proteinase inhibition by SPI-3 has been demonstrated, and mutations within the serpin reactive center loop (RCL) do not affect the ability to regulate cell f...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2000.0377
更新日期:2000-07-05 00:00:00
abstract::To begin to map functional domains of the Sendai P-L RNA polymerase complex we wanted to characterize the P binding site on the Sendai L protein. Analysis of in vitro and in vivo P-L polymerase complex formation with carboxyl-truncations of the L protein showed that the N-terminal half of the protein was required. Sit...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.0008
更新日期:1995-11-10 00:00:00
abstract::The interaction of reovirus with the cytoskeleton was investigated. The soluble components of infected cells were extracted with the nonionic detergent NP-40 in a physiological buffer, and a cytoskeletal extract was prepared from the detergent-insoluble fraction. We observed a selective association of viral-specified ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(87)90464-8
更新日期:1987-08-01 00:00:00
abstract::The transcriptive complex, the nucleocapsid with the viral RNA-synthesizing activity, of Newcastle disease virus (NDV) contains three protein components, the major structural subunit (NP) and two associated proteins (P and L) involved in the RNA synthesis. We studied the pathway of these proteins from synthesis to ass...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90132-1
更新日期:1985-12-01 00:00:00
abstract::Human papillomaviruses (HPVs) infect epithelial cells and are associated with genital carcinoma. Most epithelial cell lines express cell-surface glycosaminoglycans (GAGs) usually found attached to the protein core of proteoglycans. Our aim was to study how GAGs influenced HPV entry. Using a human keratinocyte cell lin...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(03)00114-4
更新日期:2003-05-25 00:00:00
abstract::Respiratory syncytial virus is a major cause of bronchiolitis in infants and pneumonia virus of mice (PVM) causes similar disease in mice. The impact of PVM infection in BALB/c and C57BL/6 neonates, and upon re-infection as elderly mice, was compared. As previously shown for adult mice, PVM caused more disease in BALB...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2015.07.019
更新日期:2015-11-01 00:00:00
abstract::A novel virus isolated from the feather follicles of cockatoos diagnosed as having psittacine beak and feather disease was characterized by electron microscopy, nucleic acid content, and polypeptide composition. Purified virions displayed an icosahedral symmetry, were nonenveloped, and had a mean diameter of 14 to 16 ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90513-8
更新日期:1989-07-01 00:00:00
abstract::Bombyx mori nucleopolyhedrovirus (BmNPV) orf5 (Bm5) is a core gene of lepidopteran baculoviruses and encodes the protein with the conserved amino acid residues (DUF3627) in its C-terminus. Here, we found that Bm5 disruption resulted in lower titers of budded viruses and fewer numbers of occlusion bodies (OBs) in B. mo...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2016.08.032
更新日期:2016-11-01 00:00:00
abstract::We evaluated the immunogenicity and protective activity of plasmid DNA vaccines encoding the influenza virus NP gene (pNP) alone or in combination with the herpes simplex virus type 1 protein 22 gene (pVP22). Optimal immune responses were observed in BALB/c mice immunized with the combination of pVP22 plus pNP, as ass...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.04.015
更新日期:2006-10-10 00:00:00
abstract::The physical structure of the genome of feline herpesvirus-1, a major upper respiratory tract pathogen of cats, was studied. Purified FHV-1 DNA was analyzed by restriction endonuclease and gamma 5' exonuclease digestion, blot hybridization, and electron microscopy. To facilitate further studies, nine bacteriophage clo...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90439-3
更新日期:1986-10-15 00:00:00
abstract::The most recent introduction of an avian influenza A virus without reassortment into mammals occurred in 1979 when H1N1 strains could be isolated from diseased pigs in northern Europe. This newly introduced avian virus formed a stable lineage in pigs and, in the meantime, spread all over Europe. In 1991 highly pathoge...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1344
更新日期:1994-07-01 00:00:00
abstract::The adenovirus (Ad) single-stranded DNA binding protein (DBP) is a multifunctional protein. It is thought to consist of two domains, the amino-terminal domain involved in host-range determination and the carboxyl-terminal domain functioning in DNA replication and DNA binding. We have determined the nucleotide sequence...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90227-9
更新日期:1988-03-01 00:00:00
abstract::Human papillomavirus (HPV) DNA is preferentially found in spontaneous abortions, specifically residing in trophoblasts, and transfected HPV-16 DNA replicates and produces progeny in 3A trophoblasts in culture. In this study 3A trophoblasts were shown to display both HPV receptors and infection by HPV-31b and HPV-6 vir...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2003.08.020
更新日期:2003-11-25 00:00:00
abstract::Hemorrhagic fever viruses are associated with rapidly progressing severe disease with high case fatality, making them of public health and biothreat importance. Effective antivirals are not available for most of the members of this diverse group of viruses. A broad spectrum strategy for antiviral development would be ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.06.032
更新日期:2009-09-15 00:00:00
abstract::Crosslinking reagents were used to interrupt the process of filamentous phage morphogenesis and investigate the orientation in which nascent virions are extruded through the host cell membrane. Infected bacteria with emerging phage particles were crosslinked with glutaraldehyde. Immunoferritin-labeling studies on thes...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(83)90382-3
更新日期:1983-05-01 00:00:00
abstract::Marek' disease virus serotype-1, also know as Gallid herpesvirus 2 (GaHV-2), elicits T-cell lymphomas in chickens. The GaHV-2 genome encodes an oncoprotein, Meq, with similarity to the Jun/Fos family of proteins. We have previously shown that Meq homodimers are not sufficient to induce lymphomas in chickens. In this s...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.01.006
更新日期:2010-04-10 00:00:00
abstract::Maturation of the arenavirus GP precursor (GPC) involves proteolytic processing by cellular signal peptidase and the proprotein convertase subtilisin kexin isozyme 1 (SKI-1)/site 1 protease (S1P), yielding a tripartite complex comprised of a stable signal peptide (SSP), the receptor-binding GP1, and the fusion-active ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2012.10.038
更新日期:2013-02-05 00:00:00
abstract::Three full-length genomic clones (pB19-M20, pB19-FL and pB19-HG1) of parvovirus B19 were produced in different laboratories. pB19-M20 was shown to produce infectious virus. To determine the differences in infectivity, all three plasmids were tested by transfection and infection assays. All three clones were similar in...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.01.002
更新日期:2008-05-10 00:00:00