Infection, replication, and cytopathology of human papillomavirus type 31 in trophoblasts.


:Human papillomavirus (HPV) DNA is preferentially found in spontaneous abortions, specifically residing in trophoblasts, and transfected HPV-16 DNA replicates and produces progeny in 3A trophoblasts in culture. In this study 3A trophoblasts were shown to display both HPV receptors and infection by HPV-31b and HPV-6 virus resulted in de novo (increasing) HPV DNA replication in these cells (inhibited by neutralizing anti-HPV31b antibodies). Reverse transcription-polymerase chain reaction analysis revealed that E1;E4, E6, and L1 were significantly expressed at days 5 (early) and 10 (late), respectively, and in situ immunocytochemistry verified L1 protein expression. Perhaps most important, HPV 31b virus infection caused both a decrease in 3A trophoblast cell numbers in a dose-dependent manner and a low trophoblast-endometrial cell adhesion (both inhibited by neutralizing anti-HPV-31 antibodies). These data further support the hypothesis that HPVs are fully active in trophoblasts and may cause some spontaneous abortions.






You H,Liu Y,Agrawal N,Prasad CK,Chiriva-Internati M,Lowery CL,Kay HH,Hermonat PL




Has Abstract


2003-11-25 00:00:00
















  • Biology of the temperate Streptococcus thermophilus bacteriophage TP-J34 and physical characterization of the phage genome.

    abstract::The temperate Streptococcus thermophilus bacteriophage TP-J34 was identified in the lysogenic host strain J34. The majority of phage particles produced upon induction was defective and noninfectious, consisting of DNA-filled heads lacking tails. A physical map (45.6 kb) was established. Analysis of minor restriction b...


    pub_type: 杂志文章


    authors: Neve H,Freudenberg W,Diestel-Feddersen F,Ehlert R,Heller KJ

    更新日期:2003-10-10 00:00:00

  • Natural controlled HIV infection: preserved HIV-specific immunity despite undetectable replication competent virus.

    abstract::Long-term non-progressive HIV infection, characterized by low but detectable viral load and stable CD4 counts in the absence of antiviral therapy, is observed in about 5% of HIV-infected patients. Here we identified four therapy naïve individuals who are strongly seropositive for HIV-1 but who lack evidence of detecta...


    pub_type: 杂志文章


    authors: Kloosterboer N,Groeneveld PH,Jansen CA,van der Vorst TJ,Koning F,Winkel CN,Duits AJ,Miedema F,van Baarle D,van Rij RP,Brinkman K,Schuitemaker H

    更新日期:2005-08-15 00:00:00

  • The E5 gene of HPV-16 enhances keratinocyte immortalization by full-length DNA.

    abstract::Human papillomavirus type 16 (HPV-16) is the most common papillomavirus genome found in human cervical cancers and its DNA is capable of immortalizing primary keratinocytes in vitro. When expressed by their native promoter, two separate HPV-16 oncogenes, E6 and E7, cooperate to immortalize primary human keratinocytes....


    pub_type: 杂志文章


    authors: Stöppler MC,Straight SW,Tsao G,Schlegel R,McCance DJ

    更新日期:1996-09-01 00:00:00

  • Hepatitis E virus (HEV): molecular cloning and sequencing of the full-length viral genome.

    abstract::We have recently described the cloning of a portion of the hepatitis E virus (HEV) and confirmed its etiologic association with enterically transmitted (waterborne, epidemic) non-A, non-B hepatitis. The virus consists of a single-stranded, positive-sense RNA genome of approximately 7.5 kb, with a polyadenylated 3' end...


    pub_type: 杂志文章


    authors: Tam AW,Smith MM,Guerra ME,Huang CC,Bradley DW,Fry KE,Reyes GR

    更新日期:1991-11-01 00:00:00

  • Detection of visna virus antigens and RNA in glial cells in foci of demyelination.

    abstract::Visna is a slow virus infection of sheep in which the characteristic pathological change is demyelination in foci of inflammation. The latter is thought to be the result of an immunopathological process directed against cellular and antigenic targets that have been difficult to define because of restricted viral gene ...


    pub_type: 杂志文章


    authors: Stowring L,Haase AT,Petursson G,Georgsson G,Palsson P,Lutley R,Roos R,Szuchet S

    更新日期:1985-03-01 00:00:00

  • Hepatitis delta antigen expressed by recombinant baculoviruses: comparison of biochemical properties and post-translational modifications between the large and small forms.

    abstract::Hepatitis delta virus (HDV) encodes only one protein, the hepatitis delta antigen (HDAg). Two forms of HDAg, a large (27 kDa) and a small (24 kDa) one, participate in the various steps of HDV replication. To further understand the properties of HDAg, we have constructed recombinant baculoviruses and expressed both for...


    pub_type: 杂志文章


    authors: Hwang SB,Lee CZ,Lai MM

    更新日期:1992-09-01 00:00:00

  • Role of matrix protein in the type D retrovirus replication cycle: importance of the arginine residue at position 55.

    abstract::We previously reported that a mutant of Mason-Pfizer monkey virus (M-PMV), which has an amino acid substitution in the matrix (MA) protein at position 55, MA-R55W, showed altered viral morphogenesis, reduced glycoprotein incorporation, and loss of infectivity. In this report, we show that two additional amino acid sub...


    pub_type: 杂志文章


    authors: Yasuda J,Hunter E

    更新日期:2000-03-15 00:00:00

  • Characterization of a novel alphaherpesvirus associated with fatal infections of domestic rabbits.

    abstract::A virus was found to be associated with a severe disease affecting rabbits on a farm near Anchorage, Alaska. Extracts from the skin of infected rabbits produced syncytia and cell lysis in cultured rabbit skin, rabbit kidney, and Vero cells. Examination of the infectious agent by electron microscopy revealed an icosahe...


    pub_type: 杂志文章


    authors: Jin L,Löhr CV,Vanarsdall AL,Baker RJ,Moerdyk-Schauwecker M,Levine C,Gerlach RF,Cohen SA,Alvarado DE,Rohrmann GF

    更新日期:2008-08-15 00:00:00

  • Cross-reactive monoclonal antibodies to multiple HIV-1 subtype and SIVcpz envelope glycoproteins.

    abstract::The extraordinarily high level of genetic variation of HIV-1 env genes poses a challenge to obtain antibodies that cross-react with multiple subtype Env glycoproteins. To determine if cross-reactive monoclonal antibodies (mAbs) to highly conserved epitopes in HIV-1 envelope glycoproteins can be induced, we immunized m...


    pub_type: 杂志文章


    authors: Gao F,Scearce RM,Alam SM,Hora B,Xia S,Hohm JE,Parks RJ,Ogburn DF,Tomaras GD,Park E,Lomas WE,Maino VC,Fiscus SA,Cohen MS,Moody MA,Hahn BH,Korber BT,Liao HX,Haynes BF

    更新日期:2009-11-10 00:00:00

  • Residues in the PB2 and PA genes contribute to the pathogenicity of avian H7N3 influenza A virus in DBA/2 mice.

    abstract::Replication and transmission of avian influenza virus in humans poses a pandemic threat. The molecular determinants that facilitate this process are not well understood. We used DBA/2 mice to identify viral factors that mediate the difference in pathogenesis between a virulent (H7N3) and a non-virulent (H7N9) avian in...


    pub_type: 杂志文章


    authors: DesRochers BL,Chen RE,Gounder AP,Pinto AK,Bricker T,Linton CN,Rogers CD,Williams GD,Webby RJ,Boon AC

    更新日期:2016-07-01 00:00:00

  • Production and characterization of monoclonal antibodies to porcine group C rotaviruses cross-reactive with group A rotaviruses.

    abstract::Five monoclonal antibodies (MAbs) to porcine group (gp) C rotaviruses (Cowden and Ah strains) reactive with both gp A and C rotaviruses in cell culture immunofluorescence (CCIF) tests were produced and characterized. These MAbs reacted with three strains of gp A and two strains of gp C rotaviruses in a CCIF test and w...


    pub_type: 杂志文章


    authors: Tsunemitsu H,Ojeh CK,Jiang B,Simkins RA,Weilnau PA,Saif LJ

    更新日期:1992-11-01 00:00:00

  • Monoclonal antibodies raised to purified woodchuck hepatitis virus core antigen particles demonstrate X antigen reactivity.

    abstract::Woodchuck hepatitis core antigen (WHcAg) particles purified from the liver of chronically infected animals were used for monoclonal antibody production. Most of the putative clones demonstrated anti-WHc specificity. However, the supernatants from several putative clones bound X antigen sequences from woodchuck hepatit...


    pub_type: 杂志文章


    authors: Feitelson MA,Clayton MM,Phimister B

    更新日期:1990-07-01 00:00:00

  • Inefficient entry of vicriviroc-resistant HIV-1 via the inhibitor-CCR5 complex at low cell surface CCR5 densities.

    abstract::HIV-1 variants resistant to small molecule CCR5 inhibitors such as vicriviroc (VVC) have modified Env complexes that can use both the inhibitor-bound and -free forms of the CCR5 co-receptor to enter target cells. However, entry via the inhibitor-CCR5 complex is inefficient in some, but not all, cell types, particularl...


    pub_type: 杂志文章


    authors: Pugach P,Ray N,Klasse PJ,Ketas TJ,Michael E,Doms RW,Lee B,Moore JP

    更新日期:2009-05-10 00:00:00

  • Immunodetection in vivo of beet necrotic yellow vein virus-encoded proteins.

    abstract::Open reading frames identified on the four genomic RNAs of beet necrotic yellow vein virus were cloned into bacterial expression vectors and resulting cl-fusion proteins expressed in Escherichia coli were used to raise polyclonal antibodies. This set of antisera was used to show the presence of 7 of 9 predicted viral ...


    pub_type: 杂志文章


    authors: Niesbach-Klösgen U,Guilley H,Jonard G,Richards K

    更新日期:1990-09-01 00:00:00

  • Apoptosis correlates with immune activation in intestinal lymphoid tissue from macaques acutely infected by a highly enteropathic simian immunodeficiency virus, SIVsmmPBj14.

    abstract::The PBj14 isolate of simian immunodeficiency virus, SIVsmmPBj14, induces an acutely lethal disease in experimentally inoculated pigtailed macaques, that is characterized by severe enteropathy and extensive immune activation, particularly within gut-associated lymphoid tissue (GALT). Experiments were conducted to deter...


    pub_type: 杂志文章


    authors: Gummuluru S,Novembre FJ,Lewis M,Gelbard HA,Dewhurst S

    更新日期:1996-11-01 00:00:00

  • A subtype of human papillomavirus 5 (HPV-5b) and its subgenomic segment amplified in a carcinoma: nucleotide sequences and genomic organizations.

    abstract::A subtype of human papillomavirus 5 (HPV-5b) is closely associated with carcinomas in the disease epidermodysplasia verruciformis (EV). The complete genome was cloned from virus particles in benign lesions of a patient with EV and sequenced: it was 7779 nucleotides long and consisted of six open reading frames (ORFs) ...


    pub_type: 杂志文章


    authors: Yabe Y,Sakai A,Hitsumoto T,Kato H,Ogura H

    更新日期:1991-08-01 00:00:00

  • Map location of lactate dehydrogenase-elevating virus (LDV) capsid protein (Vp1) gene.

    abstract::Lactate dehydrogenase-elevating virus (LDV) is currently classified within the Togaviridae family. In an effort to obtain further information on the characteristics of this virus, we have begun to sequence the viral RNA genome and to map the virion structural protein genes. A sequence of 1064 nucleotides, which repres...


    pub_type: 杂志文章


    authors: Godeny EK,Speicher DW,Brinton MA

    更新日期:1990-08-01 00:00:00

  • Construction and characterization of an infectious clone generated from Chikungunya virus SL11131 strain.

    abstract::Chikungunya virus (CHIKV) is a mosquito-borne RNA virus that causes Chikungunya fever in humans. In this study, we generated two DNA-based CHIKV infectious clones derived from an Indian Ocean Lineage SL11131 strain and a prototype Ross strain. When the replication capabilities of the infectious CHIKV in various cell l...


    pub_type: 杂志文章


    authors: Suzuki Y,Tanaka A,Maeda Y,Emi A,Fujioka Y,Sakaguchi S,Vasudevan SG,Kobayashi T,Lim CK,Takasaki T,Wu H,Nakano T

    更新日期:2021-01-02 00:00:00

  • Characterization of tail sheath protein of giant bacteriophage phiKZ Pseudomonas aeruginosa.

    abstract::The tail sheath protein of giant bacteriophage phiKZ Pseudomonas aeruginosa encoded by gene 29 was identified and its expression system was developed. Localization of the protein on the virion was confirmed by immunoelectron microscopy. Properties of gene product (gp) 29 were studied by electron microscopy, immunoblot...


    pub_type: 杂志文章


    authors: Kurochkina LP,Aksyuk AA,Sachkova MY,Sykilinda NN,Mesyanzhinov VV

    更新日期:2009-12-20 00:00:00

  • Antigen specificity of CD4 T cell response in the central nervous system of mice infected with mouse hepatitis virus.

    abstract::Previously, we showed that the transmembrane (M) and surface (S) glycoproteins were recognized by splenic CD4 T lymphocytes harvested from mice infected intraperitoneally with mouse hepatitis virus, strain JHM (MHV-JHM), whereas only the S protein was recognized by splenocytes derived from mice with MHV-induced chroni...


    pub_type: 杂志文章


    authors: Xue S,Perlman S

    更新日期:1997-11-10 00:00:00

  • Innate and acquired immunity to herpes simplex virus type 1.

    abstract::Immunization with heat-inactivated herpes simplex virus type 1 (HSV-1) 2-5 days before ocular infection reduced the frequency of establishment of latent HSV-1 infection in the trigeminal ganglion (TG); this induction of resistance coincided with reduced expression of IFN-gamma mRNA in the TG. Immunization with unrelat...


    pub_type: 杂志文章


    authors: Halford WP,Veress LA,Gebhardt BM,Carr DJ

    更新日期:1997-09-29 00:00:00

  • Increase in the relative fitness of a plant virus RNA associated with its recombinant nature.

    abstract::A recombination event at the 3' end of RNA 3 in a pseudorecombinant virus (C1C2T3) having RNAs 1 and 2 from TrK7-cucumber mosaic virus (CMV) and RNA 3 from P-tomato aspermy virus (TAV) was detected by ribonuclease protection assay (RPA). Sequence analysis of the 3' end of RNA 3 of C1C2T3 and of its parental CMV and TA...


    pub_type: 杂志文章


    authors: Fernández-Cuartero B,Burgyán J,Aranda MA,Salánki K,Moriones E,García-Arenal F

    更新日期:1994-09-01 00:00:00

  • The role of PI3K/Akt in human herpesvirus infection: From the bench to the bedside.

    abstract::The phosphatidylinositol-3-kinase (PI3K)-Akt signaling pathway regulates several key cellular functions including protein synthesis, cell growth, glucose metabolism, and inflammation. Many viruses have evolved mechanisms to manipulate this signaling pathway to ensure successful virus replication. The human herpesvirus...


    pub_type: 杂志文章,评审


    authors: Liu X,Cohen JI

    更新日期:2015-05-01 00:00:00

  • Cellular Hsp27 interacts with classical swine fever virus NS5A protein and negatively regulates viral replication by the NF-κB signaling pathway.

    abstract::Classical swine fever virus (CSFV) nonstructural protein NS5A is a multifunctional protein functioning in regulation of viral genome replication, protein translation and assembly by interaction with viral or host proteins. Here, heat shock protein 27 (Hsp27) has been identified as a novel binding partner of NS5A by us...


    pub_type: 杂志文章


    authors: Ling S,Luo M,Jiang S,Liu J,Ding C,Zhang Q,Guo H,Gong W,Tu C,Sun J

    更新日期:2018-05-01 00:00:00

  • Cell-mediated immune responses to COPV early proteins.

    abstract::Cell-mediated immunity plays a key role in the regression of papillomavirus-induced warts and intra-epithelial lesions but the target antigens that induce this response are not clear. Canine oral papillomavirus (COPV) infection of the oral cavity in dogs is a well-characterized model of mucosal papillomavirus infectio...


    pub_type: 杂志文章


    authors: Jain S,Moore RA,Anderson DM,Gough GW,Stanley MA

    更新日期:2006-12-05 00:00:00

  • Characterization of the leader papain-like proteinase of MHV-A59: identification of a new in vitro cleavage site.

    abstract::Sequence analysis of the mouse hepatitis virus, strain A59 (MHV-A59) genome predicts the presence of two papain-like proteinases encoded within the first open reading frame (ORF 1a) of the replicase gene. The more 5' of these domains, the leader papain-like proteinase, is responsible for the cleavage of the amino term...


    pub_type: 杂志文章


    authors: Bonilla PJ,Hughes SA,Piñón JD,Weiss SR

    更新日期:1995-06-01 00:00:00

  • The secretory cells of mouse salivary glands are nonpermissive for polyomavirus replication.

    abstract::Parotid tumor agent was an early name for polyomavirus due to its ability to induce tumors (myoepitheliomas originating from the myoepithelial glandular tissues) in mice inoculated with polyomavirus (Py) as neonates. It has long been thought that these tumors directly follow productive infection by Py in epithelial ce...


    pub_type: 杂志文章


    authors: Atencio IA,Meraz A,Villarreal LP

    更新日期:1994-05-01 00:00:00

  • Immunoprotection against influenza H5N1 virus by oral administration of enteric-coated recombinant Lactococcus lactis mini-capsules.

    abstract::Edible vaccines that can be made widely available and easily administered could bring great benefit to the worldwide battle against pandemic viral infections. They can be used not only for the vaccination of humans and domesticated animals, but also for wild herds and live stock which are otherwise difficult to vaccin...


    pub_type: 杂志文章


    authors: Lei H,Xu Y,Chen J,Wei X,Lam DM

    更新日期:2010-11-25 00:00:00

  • The HSV-1 2-kb latency-associated transcript is found in the cytoplasm comigrating with ribosomal subunits during productive infection.

    abstract::We have examined the nuclear and cytoplasmic distribution of the latency-associated transcripts (LATs) of HSV-1. During latency these transcripts accumulate in the nuclei of neurons in the peripheral and central nervous system of infected animals. However, our Northern blot analyses demonstrate that the 2-kb LAT is fo...


    pub_type: 杂志文章


    authors: Nicosia M,Zabolotny JM,Lirette RP,Fraser NW

    更新日期:1994-11-01 00:00:00

  • Mapping of linear antigenic sites on the S glycoprotein of a neurotropic murine coronavirus with synthetic peptides: a combination of nine prediction algorithms fails to identify relevant epitopes and peptide immunogenicity is drastically influenced by th

    abstract::The elucidation of the antigenic structure of the S glycoprotein of murine coronaviruses will provide further understanding of the complex pathogenicity of these viruses. In order to identify linear antigenic determinants, the primary structure of the S glycoprotein of murine hepatitis virus strain A59 was analyzed wi...


    pub_type: 杂志文章


    authors: Daniel C,Lacroix M,Talbot PJ

    更新日期:1994-08-01 00:00:00