Infection, replication, and cytopathology of human papillomavirus type 31 in trophoblasts.

Abstract:

:Human papillomavirus (HPV) DNA is preferentially found in spontaneous abortions, specifically residing in trophoblasts, and transfected HPV-16 DNA replicates and produces progeny in 3A trophoblasts in culture. In this study 3A trophoblasts were shown to display both HPV receptors and infection by HPV-31b and HPV-6 virus resulted in de novo (increasing) HPV DNA replication in these cells (inhibited by neutralizing anti-HPV31b antibodies). Reverse transcription-polymerase chain reaction analysis revealed that E1;E4, E6, and L1 were significantly expressed at days 5 (early) and 10 (late), respectively, and in situ immunocytochemistry verified L1 protein expression. Perhaps most important, HPV 31b virus infection caused both a decrease in 3A trophoblast cell numbers in a dose-dependent manner and a low trophoblast-endometrial cell adhesion (both inhibited by neutralizing anti-HPV-31 antibodies). These data further support the hypothesis that HPVs are fully active in trophoblasts and may cause some spontaneous abortions.

journal_name

Virology

journal_title

Virology

authors

You H,Liu Y,Agrawal N,Prasad CK,Chiriva-Internati M,Lowery CL,Kay HH,Hermonat PL

doi

10.1016/j.virol.2003.08.020

subject

Has Abstract

pub_date

2003-11-25 00:00:00

pages

281-9

issue

2

eissn

0042-6822

issn

1096-0341

pii

S0042682203006081

journal_volume

316

pub_type

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