Abstract:
:Urine and breast milk represent the main routes of human cytomegalovirus (HCMV) transmission but the contribution of renal and mammary epithelial cells to viral excretion remains unclear. We observed that kidney and mammary epithelial cells were permissive to HCMV infection and expressed immediate early, early and late antigens within 72 h of infection. During the first 24 h after infection, high titers of infectious virus were measured associated to the cells and in culture supernatants, independently of de novo synthesis of virus progeny. This phenomenon was not observed in HCMV-infected fibroblasts and suggested the sequestration and the release of HCMV by epithelial cells. This hypothesis was supported by confocal and electron microscopy analyses. The sequestration and progressive release of HCMV by kidney and mammary epithelial cells may play an important role in the excretion of the virus in urine and breast milk and may thereby contribute to HCMV transmission.
journal_name
Virologyjournal_title
Virologyauthors
Twite N,Andrei G,Kummert C,Donner C,Perez-Morga D,De Vos R,Snoeck R,Marchant Adoi
10.1016/j.virol.2014.04.032subject
Has Abstractpub_date
2014-07-01 00:00:00pages
55-65eissn
0042-6822issn
1096-0341pii
S0042-6822(14)00155-Xjournal_volume
460-461pub_type
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