Insertion and deletion mutants of vaccinia virus.

abstract：:A nonaphid transmissible (NAT) variant of tobacco vein mottling virus (TVMV) was used to test the hypothesis that the viral coat protein (CP) plays a role in determining aphid transmissibility. Comparison of the nucleotide sequences in the coat protein cistron of an aphid transmissible isolate (TVMV-AT) with that of T...

journal_title：Virology

authors： Atreya CD,Raccah B,Pirone TP

更新日期：1990-09-01 00:00:00

abstract：:Studies of the cellular autophagy pathway have exploded over the past twenty years. Now appreciated as a constitutive degradative mechanism that promotes cellular homeostasis, autophagy is also required for a variety of developmental processes, cellular stress responses, and immune pathways. Autophagy certainly acts a...

journal_title：Virology

authors： Jackson WT

更新日期：2015-05-01 00:00:00

abstract：:We analyzed various mutants of tomato leaf curl virus-India to investigate the role of ORFs AV3, AV2, and coat protein (CP) in viral replication, movement, and symptom development. The results of these studies indicate that ORF AV3 does not encode a protein. Plants inoculated with infectious DNA which contained deleti...

journal_title：Virology

authors： Padidam M,Beachy RN,Fauquet CM

更新日期：1996-10-15 00:00:00

abstract：:During the productive phase of chronic hepadnaviral infections, virion DNA synthesis occurs in the cytoplasm of the infected hepatocyte, but viral RNA is synthesized in the nucleus, apparently from a covalently closed, circular (CCC) viral DNA. J. Tuttleman, C. Pourcel, and J. Summers (1986a, Cell 47, 451-460) have sh...

journal_title：Virology

authors： Wu TT,Coates L,Aldrich CE,Summers J,Mason WS

更新日期：1990-03-01 00:00:00

abstract：:The differential symptom determinants of the Holmes' masked (M) and U1 strains of tobacco mosaic virus previously were mapped to the 5'-coterminal open reading frame (ORF) encoding the 126-kDa protein and the N-terminal two-thirds of the 183-kDa protein. Both proteins influence viral RNA accumulation, but the function...

journal_title：Virology

authors： Shintaku MH,Carter SA,Bao Y,Nelson RS

更新日期：1996-07-01 00:00:00

abstract：:Cauliflower mosaic virus gene VI product (P6) is an essential protein that forms cytoplasmic, inclusion bodies (IBs). P6 contains four regions involved in self-association, termed D1-D4. D3 binds to D1, along with D4 and contains a spacer region (termed D3b) between two RNA-binding domains. Here we show D3b binds full...

journal_title：Virology

authors： Lutz L,Okenka G,Schoelz J,Leisner S

更新日期：2015-02-01 00:00:00

abstract：:The RNA dependent RNA polymerase of Sendai virus consists of a complex of the large (L) and phosphoprotein (P) subunits where L is thought to be responsible for all the catalytic activities necessary for viral RNA synthesis. We previously showed that the L protein forms an oligomer [Smallwood, S., Cevik, B., Moyer, S....

journal_title：Virology

authors： Cevik B,Smallwood S,Moyer SA

更新日期：2007-06-20 00:00:00

abstract：:The human papillomavirus type 16 E1--E4 protein is expressed abundantly in cells supporting viral DNA amplification, but its expression is lost during malignant progression. In cell culture, 16E1--E4 causes G2 cell cycle arrest by associating with and preventing the nuclear entry of Cdk1/cyclin B1 complexes. Here, we ...

journal_title：Virology

authors： Davy CE,Ayub M,Jackson DJ,Das P,McIntosh P,Doorbar J

更新日期：2006-05-25 00:00:00

abstract：:A number of studies examining interactions of dendritic cell (DC)-specific ICAM-3 grabbing nonintegrin (DC-SIGN) with viral pathogens have relied on monocytic transfectants as models for primary DCs. Here we show that the presumed "THP-1" monocytic cells used in these studies are instead Raji B cells. Moreover, we dem...

journal_title：Virology

authors： Wu L,Martin TD,Carrington M,KewalRamani VN

更新日期：2004-01-05 00:00:00

abstract：:Replication of hepatitis B virus (HBV) proceeds by reverse transcription of an RNA intermediate inside the viral nucleocapsid formed by the core protein. This protein contains four Cys residues which occur at equivalent positions in the core proteins of all known mammalian hepadnaviruses, suggesting that they might be...

journal_title：Virology

authors： Nassal M

更新日期：1992-09-01 00:00:00

abstract：:To gain new insights regarding the role of Ku, the DNA-PK DNA-binding component, during lentiviral DNA integration, we have investigated the HIV-1 replication in Ku80-depleted human cells. CEM4fx cells underexpressing the Ku80 factor were selected after transduction by a retroviral vector expressing the Ku80 full-leng...

journal_title：Virology

authors： Jeanson L,Subra F,Vaganay S,Hervy M,Marangoni E,Bourhis J,Mouscadet JF

更新日期：2002-08-15 00:00:00

abstract：:Previous in vitro work demonstrated the incorporation of the major inducible 70k heat shock protein (i.e., 72k HSP) into the biologically active light nucleocapsid (L-NC) variant of canine distemper virus (CDV). Here, in vitro induction of the cellular stress response, characterized by elevated cytoplasmic and intranu...

journal_title：Virology

authors： Oglesbee MJ,Kenney H,Kenney T,Krakowka S

更新日期：1993-02-01 00:00:00

abstract：:Dendrolimus punctatus tetravirus (DpTV) belongs to the genus omegatetravirus of the Alphatetraviridae family. Sequence analysis predicts that DpTV replicase contains a putative helicase domain (Hel). However, the helicase activity in alphatetraviruses has never been formally determined. In this study, we determined th...

journal_title：Virology

authors： Wang Q,Han Y,Qiu Y,Zhang S,Tang F,Wang Y,Zhang J,Hu Y,Zhou X

更新日期：2012-11-25 00:00:00

abstract：:The nucleotide sequence of gene 6 encoding the rotavirus major capsid protein VP6 of EDIM strain (EW) was determined and compared to that of 20 previously reported strains with known subgroup specificities. Multiple alignments of amino acid sequences exhibited a high level of sequence conservation (87 to 99.2%). Site-...

journal_title：Virology

authors： Tang B,Gilbert JM,Matsui SM,Greenberg HB

更新日期：1997-10-13 00:00:00

abstract：:An open reading frame which encodes at least 90% of the adenovirus type 2 DNA polymerase gene was cloned behind the SP6 promoter and transcribed in vitro using the SP6 RNA polymerase. The resultant RNA was translated in a rabbit reticulocyte cell free system. In addition to the translation of a 120-kDa protein corresp...

journal_title：Virology

authors： Hassin D,Korn R,Horwitz MS

更新日期：1986-11-01 00:00:00

abstract：:The molecular cloning of the DNA provirus of Mason-Pfizer monkey virus (M-PMV) is described. Fourteen independent clones of integrated M-PMV proviruses were isolated from a human embryo kidney cell line that had been previously derived from a single cell clone infected with M-PMV. Characterization of these clones for ...

journal_title：Virology

authors： Barker CS,Pickel J,Tainsky M,Hunter E

更新日期：1986-09-01 00:00:00

abstract：:The SARS-coronavirus (SARS-CoV) is the etiological agent of severe acute respiratory syndrome (SARS). The SARS-CoV spike (S) glycoprotein mediates membrane fusion events during virus entry and virus-induced cell-to-cell fusion. To delineate functional domains of the SARS-CoV S glycoprotein, single point mutations, clu...

journal_title：Virology

authors： Petit CM,Melancon JM,Chouljenko VN,Colgrove R,Farzan M,Knipe DM,Kousoulas KG

更新日期：2005-10-25 00:00:00

abstract：:Tomato golden mosaic virus (TGMV) is a bipartite member of the subgroup III Geminiviridae. Like all geminiviruses, TGMV replicates in the nucleus of susceptible cells by rolling circle replication (RCR). Double-stranded replicative form DNA generated during RCR serves as template for the transcription of viral genes b...

journal_title：Virology

authors： Sunter G,Bisaro DM

更新日期：1997-06-09 00:00:00

abstract：:nsP3 is one of four viral nonstructural proteins required for RNA replication of Sindbis virus. In this report, post-translational modifications of nsP3 which occur in both vertebrate and mosquito cell cultures have been examined. In pulse-chase experiments, analyzed by immunoprecipitation and sodium dodecyl sulfate-p...

journal_title：Virology

authors： Li GP,La Starza MW,Hardy WR,Strauss JH,Rice CM

更新日期：1990-11-01 00:00:00

abstract：:We report here the nonenzymatic self-ligation of transcripts corresponding to the peach latent mosaic viroid (PLMVd). This is the first description of this process with viroid sequences, although it has been reported to occur with human hepatitis delta virus RNA. Self-ligation occurs when the 5'-hydroxyl and the 2',3'...

journal_title：Virology

authors： Lafontaine D,Beaudry D,Marquis P,Perreault JP

更新日期：1995-10-01 00:00:00

abstract：:The virion-associated Vpr protein of human immunodeficiency virus type 1 (HIV-1) alters cell cycle progression from the G2 phase, influences the virus in vivo mutation rate, and participates in the nuclear translocation of viral DNA. While many Vpr-interacting proteins have been identified, the functional relevance of...

journal_title：Virology

authors： Mansky LM,Preveral S,Le Rouzic E,Bernard LC,Selig L,Depienne C,Benarous R,Benichou S

更新日期：2001-03-30 00:00:00

abstract：:The infectivity in vivo, replication competence in vitro, and expression of viral genes of several molecularly cloned duck hepatitis B virus (DHBV) genomes were investigated. In addition, replication competence, core protein expression, and secretion of viral proteins were investigated for a grey heron hepatitis B vir...

journal_title：Virology

authors： Wildner G,Fernholz D,Sprengel R,Schneider R,Will H

更新日期：1991-11-01 00:00:00

abstract：:Although it is known that Ad5-specific neutralizing antibodies (NAbs) against three major viral capsid components (hexon, penton and fiber) are generated, differences in the frequency and nature of these pre-existing NAbs remain unclear. The results emphasized the contribution of anti-fiber antibodies to Ad5 neutraliz...

journal_title：Virology

authors： Yu B,Dong J,Wang C,Zhan Y,Zhang H,Wu J,Kong W,Yu X

更新日期：2013-03-15 00:00:00

abstract：:The role of oxygen tension level is a well-known phenomenon that has been studied in oncology and radiotherapy since about 60 years. Oxygen tension may inhibit or stimulate propagation of viruses in vitro as well as in vivo. In turn modulating oxygen metabolism may constitute a novel approach to treat viral infections...

journal_title：Virology

authors： Morinet F,Casetti L,François JH,Capron C,Pillet S

更新日期：2013-09-01 00:00:00

abstract：:The HCMV IE2 protein promiscuously activates transcription of many viral and cellular genes. IE2 also negatively autoregulates its own expression by binding to a strategically positioned IE2 binding site, called CRS, located immediately downstream of the TATA box of the HCMV major IE promoter. Here we show that IE2 is...

journal_title：Virology

authors： Tsai HL,Kou GH,Tang FM,Wu CW,Lin YS

更新日期：1997-11-24 00:00:00

abstract：:The complete nucleotide sequence of the RSV cpts-248/404 live attenuated vaccine candidate was determined from cloned cDNA and was compared to that of the RSV A2/HEK7 wild-type, cold-passaged cp-RSV, and cpts-248 virus, which constitute the series of progenitor viruses. RSV cpts-248/404 is more attenuated and more tem...

journal_title：Virology

authors： Firestone CY,Whitehead SS,Collins PL,Murphy BR,Crowe JE Jr

更新日期：1996-11-15 00:00:00

abstract：:Tumor-inducing viruses like simian virus 40 or the human adenovirus produce oncoproteins which interfere with the cellular retinoblastoma (Rb) tumor-suppressor protein to create an appropriate molecular environment in the nucleus for viral transcription and replication. Such a strategy has been considered to be restri...

journal_title：Virology

authors： Collin S,Fernández-Lobato M,Gooding PS,Mullineaux PM,Fenoll C

更新日期：1996-05-01 00:00:00

abstract：:The complete DNA sequence of a new lytic T7-like bacteriophage phiKMV is presented. It is the first genome sequence of a member of the Podoviridae that infects Pseudomonas aeruginosa. The linear G + C-rich (62.3%) double-stranded DNA genome of 42,519 bp has direct terminal repeats of 414 bp and contains 48 open readin...

journal_title：Virology

authors： Lavigne R,Burkal'tseva MV,Robben J,Sykilinda NN,Kurochkina LP,Grymonprez B,Jonckx B,Krylov VN,Mesyanzhinov VV,Volckaert G

更新日期：2003-07-20 00:00:00

abstract：:We previously described a single-cycle dengue vaccine (RepliVAX D2) engineered from a capsid (C) gene-deleted West Nile virus (WNV) expressing dengue virus serotype 2 (DENV2) prM/E genes in place of the corresponding WNV genes. That work demonstrated that adaptation of RepliVAX D2 to grow in WNV C-expressing cells res...

journal_title：Virology

authors： Winkelmann ER,Widman DG,Suzuki R,Mason PW

更新日期：2011-12-20 00:00:00

abstract：:The full-length E2 protein of human papillomavirus type 16 is believed to act as a trans-repressor of the viral p97 promoter. Previous reports have provided evidence that transcripts with the potential to encode the E2 protein contain the 880/2708 splice junction. We have further analyzed the structure of the E2-encod...

journal_title：Virology

authors： Sherman L,Alloul N

更新日期：1992-12-01 00:00:00