Abstract:
:The nucleotide sequence of gene 6 encoding the rotavirus major capsid protein VP6 of EDIM strain (EW) was determined and compared to that of 20 previously reported strains with known subgroup specificities. Multiple alignments of amino acid sequences exhibited a high level of sequence conservation (87 to 99.2%). Site-specific mutagenesis experiments were undertaken to localize regions involved in subgroup specificity. Amino acid positions 305, 315, and a region 296-299 (or 301 for equine strain H-2) were identified as contributing to subgroup epitopes. A single amino acid mutation at position 305 or 315 was sufficient to change the subgroup specificity of EW VP6 protein from non I/II to subgroup I- or subgroup II-like, respectively. Mutation at these sites may be another important mechanism for subgroup variation, along with gene reassortment.
journal_name
Virologyjournal_title
Virologyauthors
Tang B,Gilbert JM,Matsui SM,Greenberg HBdoi
10.1006/viro.1997.8762subject
Has Abstractpub_date
1997-10-13 00:00:00pages
89-96issue
1eissn
0042-6822issn
1096-0341pii
S0042-6822(97)98762-6journal_volume
237pub_type
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