Abstract:
:Human papillomavirus type 16 (HPV-16) is the most common papillomavirus genome found in human cervical cancers and its DNA is capable of immortalizing primary keratinocytes in vitro. When expressed by their native promoter, two separate HPV-16 oncogenes, E6 and E7, cooperate to immortalize primary human keratinocytes. Early HPV cervical lesions express abundant amounts of E5-specific RNA, and using a quantitative keratinocyte immortalization assay, we demonstrate here that E5 can act in cis to increase (4-10 fold) the efficiency of cellular immortalization by E6/E7. These results suggest that the expression of the HPV-16 E5 gene may play a role in the pathogenesis of early HPV infections by potentiating the effects of E6/E7.
journal_name
Virologyjournal_title
Virologyauthors
Stöppler MC,Straight SW,Tsao G,Schlegel R,McCance DJdoi
10.1006/viro.1996.0475subject
Has Abstractpub_date
1996-09-01 00:00:00pages
251-4issue
1eissn
0042-6822issn
1096-0341pii
S0042-6822(96)90475-4journal_volume
223pub_type
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