Abstract:
:The nucleotide sequence of a highly pathogenic porcine reproductive and respiratory syndrome virus (PRRSV) was determined. Transfection of MARC-145 cells with capped in vitro transcripts derived from a full-length cDNA clone of the viral genome resulted in infectious PRRSV with growth characteristics similar to that of the parental virus. Primer extension analysis revealed that during replication, the viral polymerase corrected the two nonviral guanosine residues present at the 5' terminus of the transfected transcripts. Animal studies showed that the cloned virus induced hyperthermia, persistent viremia, and antibody response, similar to that observed with the parental virus. Contact transmission occurred rapidly within 3 days of introduction of naïve pigs into the group of clone virus-inoculated pigs. These results suggest that the cloned virus retains the in vivo virulence and contagion properties of the parental virus, thus, providing the background for reverse genetics manipulation in systematic examination of attenuation and virulence phenotypes.
journal_name
Virologyjournal_title
Virologyauthors
Truong HM,Lu Z,Kutish GF,Galeota J,Osorio FA,Pattnaik AKdoi
10.1016/j.virol.2004.04.046subject
Has Abstractpub_date
2004-08-01 00:00:00pages
308-19issue
2eissn
0042-6822issn
1096-0341pii
S0042682204003514journal_volume
325pub_type
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