Abstract:
:Given dengue virus (DENV) genome austerity, it uses cellular molecules and structures for virion entry, translation and replication of the genome. NS1 is a multifunctional protein key to viral replication and pathogenesis. Identification of cellular proteins that interact with NS1 may help in further understanding the functions of NS1. In this paper we isolated a total of 64 proteins from DENV infected human hepatic cells (Huh-7) that interact with NS1 by affinity chromatography and immunoprecipitation assays. The subcellular location and expression levels during infection of the ribosomal proteins RPS3a, RPL7, RPL18, RPL18a plus GAPDH were determined. None of these proteins changed their expression levels during infection; however, RPL-18 was redistributed to the perinuclear region after 48hpi. Silencing of the RPL-18 does not affect cell translation efficiency or viability, but it reduces significantly viral translation, replication and viral yield, suggesting that the RPL-18 is required during DENV replicative cycle.
journal_name
Virologyjournal_title
Virologyauthors
Cervantes-Salazar M,Angel-Ambrocio AH,Soto-Acosta R,Bautista-Carbajal P,Hurtado-Monzon AM,Alcaraz-Estrada SL,Ludert JE,Del Angel RMdoi
10.1016/j.virol.2015.05.017subject
Has Abstractpub_date
2015-10-01 00:00:00pages
113-126eissn
0042-6822issn
1096-0341pii
S0042-6822(15)00273-1journal_volume
484pub_type
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