Abstract:
:The sid (size determination) gene product of phage P4 is known to be involved in capsid-size determination. Moreover, the capsid-size determination function interferes with the lytic development of its helper P2, presumably because the helper genome is too large to be packaged into P4-size capsids. In order to study P4-specified helper interference, we cloned the sid gene for expression during phage infection. Even though gpSid restores the capsid-size determination function of a sid defective P4 mutant, we find that gpSid alone is not sufficient to establish full interference of helper P2 phage production. Complete helper interference requires some P4 function in addition to gpSid. Complementation tests show that none of the known P4 genes display this property. We propose that P4 encodes a yet-unidentified function that in concert with gpSid establishes full P2 helper interference at the level of capsid-size determination.
journal_name
Virologyjournal_title
Virologyauthors
Nilssen O,Fossdal CG,Johansen BV,Lindqvist BHdoi
10.1006/viro.1996.0270subject
Has Abstractpub_date
1996-05-15 00:00:00pages
443-52issue
2eissn
0042-6822issn
1096-0341pii
S0042-6822(96)90270-6journal_volume
219pub_type
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